2w8j - Revision history

OCA at 10:09, 9 May 2024

2024-05-09T10:09:11Z

←Older revision		Revision as of 10:09, 9 May 2024
Line 3:		Line 3:
	<StructureSection load='2w8j' size='340' side='right'caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2w8j' size='340' side='right'caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W8J FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PLS:~~[~~3-HYDROXY-2-METHYL-~~5~~-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2jgt\|2jgt]], [[2jg2\|2jg2]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [https://pdbe.org/2w8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [https://www.ebi.ac.uk/pdbsum/2w8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [https://pdbe.org/2w8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [https://www.ebi.ac.uk/pdbsum/2w8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPT_SPHPI SPT_SPHPI] Catalyzes the condensation of L-serine with palmitoyl-CoA (hexadecanoyl-CoA) to produce 3-oxosphinganine (PubMed:11279212, PubMed:17557831, PubMed:17559874, PubMed:19376777). Exhibits a broad substrate specificity concerning the chain length and the degree of unsaturation of acyl-CoA (PubMed:11279212, PubMed:19376777).<ref>PMID:11279212</ref> <ref>PMID:17557831</ref> <ref>PMID:17559874</ref> <ref>PMID:19376777</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus devorans zimmermann 1890]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Campopiano, D J~~]]	+	[[Category: Sphingomonas paucimobilis]]
-	[[Category: ~~Carter, L G~~]]	+	[[Category: Campopiano DJ]]
-	[[Category: ~~Johnson, K A~~]]	+	[[Category: Carter LG]]
-	[[Category: ~~Naismith, J H~~]]	+	[[Category: Johnson KA]]
-	[[Category: ~~Raman, M C.C~~]]	+	[[Category: Naismith JH]]
-	[[Category: ~~Transferase~~]]	+	[[Category: Raman MCC]]

OCA at 11:00, 6 April 2022

2022-04-06T11:00:22Z

←Older revision		Revision as of 11:00, 6 April 2022
Line 3:		Line 3:
	<StructureSection load='2w8j' size='340' side='right'caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2w8j' size='340' side='right'caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2W8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W8J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jgt\|2jgt]], [[2jg2\|2jg2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jgt\|2jgt]], [[2jg2\|2jg2]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [~~http~~://pdbe.org/2w8j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2w8j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [https://pdbe.org/2w8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [https://www.ebi.ac.uk/pdbsum/2w8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Serine palmitoyltransferase\|Serine palmitoyltransferase]]	+	*[[Serine palmitoyltransferase 3D structures\|Serine palmitoyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:21, 1 July 2020

2020-07-01T08:21:06Z

←Older revision		Revision as of 08:21, 1 July 2020
Line 1:		Line 1:

	==SPT with PLP-ser==		==SPT with PLP-ser==
-	<StructureSection load='2w8j' size='340' side='right' caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='2w8j' size='340' side='right'caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2W8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2W8J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jgt\|2jgt]], [[2jg2\|2jg2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jgt\|2jgt]], [[2jg2\|2jg2]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [http://pdbe.org/2w8j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [http://www.ebi.ac.uk/pdbsum/2w8j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [http://pdbe.org/2w8j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [http://www.ebi.ac.uk/pdbsum/2w8j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus devorans zimmermann 1890]]		[[Category: Bacillus devorans zimmermann 1890]]
		+	[[Category: Large Structures]]
	[[Category: Campopiano, D J]]		[[Category: Campopiano, D J]]
	[[Category: Carter, L G]]		[[Category: Carter, L G]]

OCA at 08:54, 26 September 2018

2018-09-26T08:54:52Z

←Older revision		Revision as of 08:54, 26 September 2018
Line 1:		Line 1:

-	==SPT ~~WITH~~ PLP-~~SER~~==	+	==SPT with PLP-ser==
	<StructureSection load='2w8j' size='340' side='right' caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2w8j' size='340' side='right' caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w8/2w8j_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w8/2w8j_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 12:29, 11 August 2016

2016-08-11T12:29:36Z

←Older revision		Revision as of 12:29, 11 August 2016
Line 1:		Line 1:
		+
	==SPT WITH PLP-SER==		==SPT WITH PLP-SER==
	<StructureSection load='2w8j' size='340' side='right' caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2w8j' size='340' side='right' caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sphingomonas_paucimobilis Sphingomonas paucimobilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W8J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jgt\|2jgt]], [[2jg2\|2jg2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jgt\|2jgt]], [[2jg2\|2jg2]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [http://www.ebi.ac.uk/pdbsum/2w8j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [http://pdbe.org/2w8j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [http://www.ebi.ac.uk/pdbsum/2w8j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w8j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2w8j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sphingomonas paucimobilis~~]]	+	[[Category: Bacillus devorans zimmermann 1890]]
	[[Category: Campopiano, D J]]		[[Category: Campopiano, D J]]
	[[Category: Carter, L G]]		[[Category: Carter, L G]]

OCA at 16:12, 19 January 2015

2015-01-19T16:12:27Z

←Older revision		Revision as of 16:12, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W8J FirstGlance]. <br>		<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W8J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jgt\|2jgt]], [[2jg2\|2jg2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jgt\|2jgt]], [[2jg2\|2jg2]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [http://www.ebi.ac.uk/pdbsum/2w8j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [http://www.ebi.ac.uk/pdbsum/2w8j PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Sphingomonas paucimobilis]]		[[Category: Sphingomonas paucimobilis]]
-	[[Category: Campopiano, D J.]]	+	[[Category: Campopiano, D J]]
-	[[Category: Carter, L G.]]	+	[[Category: Carter, L G]]
-	[[Category: Johnson, K A.]]	+	[[Category: Johnson, K A]]
-	[[Category: Naismith, J H.]]	+	[[Category: Naismith, J H]]
-	[[Category: Raman, M C.C.]]	+	[[Category: Raman, M C.C]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 08:21, 29 September 2014

2014-09-29T08:21:54Z

←Older revision		Revision as of 08:21, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2w8j.~~png~~\|~~left~~\|200px]]	+	==SPT WITH PLP-SER==
		+	<StructureSection load='2w8j' size='340' side='right' caption='[[2w8j]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2w8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W8J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLS:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE'>PLS</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jgt\|2jgt]], [[2jg2\|2jg2]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w8j RCSB], [http://www.ebi.ac.uk/pdbsum/2w8j PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w8/2w8j_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sphingolipid biosynthesis begins with the condensation of L-serine and palmitoyl-CoA catalyzed by the PLP-dependent enzyme serine palmitoyltransferase (SPT). Mutations in human SPT cause hereditary sensory autonomic neuropathy type 1, a disease characterized by loss of feeling in extremities and severe pain. The human enzyme is a membrane-bound hetereodimer, and the most common mutations are located in the enzymatically incompetent monomer, suggesting a "dominant" or regulatory effect. The molecular basis of how these mutations perturb SPT activity is subtle and is not simply loss of activity. To further explore the structure and mechanism of SPT, we have studied the homodimeric bacterial enzyme from Sphingomonas paucimobilis. We have analyzed two mutants (N100Y and N100W) engineered to mimic the mutations seen in hereditary sensory autonomic neuropathy type 1 as well as a third mutant N100C designed to mimic the wild-type human SPT. The N100C mutant appears fully active, whereas both N100Y and N100W are significantly compromised. The structures of the holoenzymes reveal differences around the active site and in neighboring secondary structure that transmit across the dimeric interface in both N100Y and N100W. Comparison of the l-Ser external aldimine structures of both native and N100Y reveals significant differences that hinder the movement of a catalytically important Arg(378) residue into the active site. Spectroscopic analysis confirms that both N100Y and N100W mutants subtly affect the chemistry of the PLP. Furthermore, the N100Y and R378A mutants appear less able to stabilize a quinonoid intermediate. These data provide the first experimental insight into how the most common disease-associated mutations of human SPT may lead to perturbation of enzyme activity.

-	~~{{STRUCTURE_2w8j\| PDB=2w8j \| SCENE= }}~~	+	The external aldimine form of serine palmitoyltransferase: structural, kinetic, and spectroscopic analysis of the wild-type enzyme and HSAN1 mutant mimics.,Raman MC, Johnson KA, Yard BA, Lowther J, Carter LG, Naismith JH, Campopiano DJ J Biol Chem. 2009 Jun 19;284(25):17328-39. Epub 2009 Apr 17. PMID:19376777<ref>PMID:19376777</ref>

-	~~===SPT WITH PLP-SER===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19376777}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2w8j]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA].~~	+

	==See Also==		==See Also==
	*[[Serine palmitoyltransferase\|Serine palmitoyltransferase]]		*[[Serine palmitoyltransferase\|Serine palmitoyltransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019376777<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Sphingomonas paucimobilis]]		[[Category: Sphingomonas paucimobilis]]
	[[Category: Campopiano, D J.]]		[[Category: Campopiano, D J.]]

OCA at 19:46, 20 October 2012

2012-10-20T19:46:20Z

←Older revision		Revision as of 19:46, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2w8j]] is a 1 chain structure ~~of [[Serine palmitoyltransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA].	+	[[2w8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA].

	==See Also==		==See Also==

OCA: Protected "2w8j" [edit=sysop:move=sysop]

2012-07-27T08:25:19Z

Protected "2w8j" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:25, 27 July 2012

OCA at 08:25, 27 July 2012

2012-07-27T08:25:17Z

←Older revision		Revision as of 08:25, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2w8j.png\|left\|200px]]		[[Image:2w8j.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2w8j", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2w8j\| PDB=2w8j \| SCENE= }}		{{STRUCTURE_2w8j\| PDB=2w8j \| SCENE= }}

	===SPT WITH PLP-SER===		===SPT WITH PLP-SER===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19376777}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19376777 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19376777}}		{{ABSTRACT_PUBMED_19376777}}

	==About this Structure==		==About this Structure==
-	~~2W8J~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA].	+	[[2w8j]] is a 1 chain structure of [[Serine palmitoyltransferase]] with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8J OCA].
		+
		+	==See Also==
		+	*[[Serine palmitoyltransferase\|Serine palmitoyltransferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19376777~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019376777</ref><references group="xtra"/>
	[[Category: Sphingomonas paucimobilis]]		[[Category: Sphingomonas paucimobilis]]
	[[Category: Campopiano, D J.]]		[[Category: Campopiano, D J.]]
Line 31:		Line 22:
	[[Category: Raman, M C.C.]]		[[Category: Raman, M C.C.]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 09:17:01 2009''