2wes - Revision history

OCA at 15:53, 13 December 2023

2023-12-13T15:53:59Z

←Older revision		Revision as of 15:53, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2wes' size='340' side='right'caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2wes' size='340' side='right'caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~'streptomyces_rugosporus' 'streptomyces~~ rugosporus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WES FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rugosporus Streptomyces rugosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WES FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [https://pdbe.org/2wes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [https://www.ebi.ac.uk/pdbsum/2wes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wes ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [https://pdbe.org/2wes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [https://www.ebi.ac.uk/pdbsum/2wes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wes ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A4D0H5_STRRG A4D0H5_STRRG]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces rugosporus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Naismith, J H~~]]	+	[[Category: Streptomyces rugosporus]]
-	[[Category: ~~Zhu, X~~]]	+	[[Category: Naismith JH]]
-	[[Category: ~~Antifungal protein]]~~	+	[[Category: Zhu X]]
-	~~[[Category: Regioselectivity]]~~	+
-	~~[[Category: Tryptophan 5-halogenase~~]]	+

OCA at 11:05, 6 April 2022

2022-04-06T11:05:38Z

←Older revision		Revision as of 11:05, 6 April 2022
Line 3:		Line 3:
	<StructureSection load='2wes' size='340' side='right'caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2wes' size='340' side='right'caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/'streptomyces_rugosporus' 'streptomyces rugosporus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WES FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/'streptomyces_rugosporus' 'streptomyces rugosporus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WES FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [~~http~~://pdbe.org/2wes PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wes PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wes ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [https://pdbe.org/2wes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [https://www.ebi.ac.uk/pdbsum/2wes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wes ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:38, 1 July 2020

2020-07-01T08:38:35Z

←Older revision		Revision as of 08:38, 1 July 2020
Line 1:		Line 1:

	==Crystal structures of mutant E46Q of tryptophan 5-halogenase (PyrH)==		==Crystal structures of mutant E46Q of tryptophan 5-halogenase (PyrH)==
-	<StructureSection load='2wes' size='340' side='right' caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='2wes' size='340' side='right'caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'streptomyces_rugosporus' 'streptomyces rugosporus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WES FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'streptomyces_rugosporus' 'streptomyces rugosporus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WES FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [http://pdbe.org/2wes PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [http://www.ebi.ac.uk/pdbsum/2wes PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wes ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [http://pdbe.org/2wes PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [http://www.ebi.ac.uk/pdbsum/2wes PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wes ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces rugosporus]]		[[Category: Streptomyces rugosporus]]
		+	[[Category: Large Structures]]
	[[Category: Naismith, J H]]		[[Category: Naismith, J H]]
	[[Category: Zhu, X]]		[[Category: Zhu, X]]

OCA at 09:27, 26 September 2018

2018-09-26T09:27:23Z

←Older revision		Revision as of 09:27, 26 September 2018
Line 1:		Line 1:

-	==~~CRYSTAL STRUCTURES OF MUTANT~~ E46Q ~~OF TRYPTOPHAN~~ 5-~~HALOGENASE~~ (~~PYRH~~)==	+	==Crystal structures of mutant E46Q of tryptophan 5-halogenase (PyrH)==
	<StructureSection load='2wes' size='340' side='right' caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2wes' size='340' side='right' caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/we/2wes_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/we/2wes_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 21:06, 11 August 2016

2016-08-11T21:06:15Z

←Older revision		Revision as of 21:06, 11 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURES OF MUTANT E46Q OF TRYPTOPHAN 5-HALOGENASE (PYRH)==		==CRYSTAL STRUCTURES OF MUTANT E46Q OF TRYPTOPHAN 5-HALOGENASE (PYRH)==
	<StructureSection load='2wes' size='340' side='right' caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2wes' size='340' side='right' caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_rugosporus Streptomyces~~ rugosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WES FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'streptomyces_rugosporus' 'streptomyces rugosporus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WES FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [http://www.ebi.ac.uk/pdbsum/2wes PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [http://pdbe.org/2wes PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [http://www.ebi.ac.uk/pdbsum/2wes PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wes ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wes ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wes" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:35, 20 January 2015

2015-01-20T11:35:26Z

←Older revision		Revision as of 11:35, 20 January 2015
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces rugosporus]]		[[Category: Streptomyces rugosporus]]
-	[[Category: Naismith, J H.]]	+	[[Category: Naismith, J H]]
-	[[Category: Zhu, X.]]	+	[[Category: Zhu, X]]
	[[Category: Antifungal protein]]		[[Category: Antifungal protein]]
	[[Category: Regioselectivity]]		[[Category: Regioselectivity]]
	[[Category: Tryptophan 5-halogenase]]		[[Category: Tryptophan 5-halogenase]]

OCA at 06:42, 10 October 2014

2014-10-10T06:42:26Z

←Older revision		Revision as of 06:42, 10 October 2014
Line 2:		Line 2:
	<StructureSection load='2wes' size='340' side='right' caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2wes' size='340' side='right' caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_rugosporus Streptomyces rugosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. <br>	+	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_rugosporus Streptomyces rugosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WES FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene~~><br>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [http://www.ebi.ac.uk/pdbsum/2wes PDBsum]</span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [http://www.ebi.ac.uk/pdbsum/2wes PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 23:		Line 22:
	Structural insights into regioselectivity in the enzymatic chlorination of tryptophan.,Zhu X, De Laurentis W, Leang K, Herrmann J, Ihlefeld K, van Pee KH, Naismith JH J Mol Biol. 2009 Aug 7;391(1):74-85. Epub 2009 Jun 6. PMID:19501593<ref>PMID:19501593</ref>		Structural insights into regioselectivity in the enzymatic chlorination of tryptophan.,Zhu X, De Laurentis W, Leang K, Herrmann J, Ihlefeld K, van Pee KH, Naismith JH J Mol Biol. 2009 Aug 7;391(1):74-85. Epub 2009 Jun 6. PMID:19501593<ref>PMID:19501593</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA: Protected "2wes" [edit=sysop:move=sysop]

2014-05-07T08:34:48Z

Protected "2wes" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:34, 7 May 2014

OCA at 08:34, 7 May 2014

2014-05-07T08:34:47Z

←Older revision		Revision as of 08:34, 7 May 2014
Line 1:		Line 1:
-	~~{{Seed}}~~	+	==CRYSTAL STRUCTURES OF MUTANT E46Q OF TRYPTOPHAN 5-HALOGENASE (PYRH)==
-	[[~~Image~~:2wes.~~png\|left~~\|200px]]	+	<StructureSection load='2wes' size='340' side='right' caption='[[2wes]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wes]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_rugosporus Streptomyces rugosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wes OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wes RCSB], [http://www.ebi.ac.uk/pdbsum/2wes PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/we/2wes_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The regioselectively controlled introduction of chlorine into organic molecules is an important biological and chemical process. This importance derives from the observation that many pharmaceutically active natural products contain a chlorine atom. Flavin-dependent halogenases are one of the principal enzyme families responsible for regioselective halogenation of natural products. Structural studies of two flavin-dependent tryptophan 7-halogenases (PrnA and RebH) have generated important insights into the chemical mechanism of halogenation by this enzyme family. These proteins comprise two modules: a flavin adenine dinucleotide (FAD)-binding module and a tryptophan-binding module. Although the 7-halogenase studies advance a hypothesis for regioselectivity, this has never been experimentally demonstrated. PyrH is a tryptophan 5-halogenase that catalyzes halogenation on tryptophan C5 position. We report the crystal structure of a tryptophan 5-halogenase (PyrH) bound to tryptophan and FAD. The FAD-binding module is essentially unchanged relative to PrnA (and RebH), and PyrH would appear to generate the same reactive species from Cl(-), O(2), and 1,5-dihydroflavin adenine dinucleotide. We report additional mutagenesis data that extend our mechanistic understanding of this process, in particular highlighting a strap region that regulates FAD binding, and may allow communication between the two modules. PyrH has a significantly different tryptophan-binding module. The data show that PyrH binds tryptophan and presents the C5 atom to the reactive chlorinating species, shielding other potential reactive sites. We have mutated residues identified by structural analysis as recognizing the tryptophan in order to confirm their role. This work establishes the method by which flavin-dependent tryptophan halogenases regioselectively control chlorine addition to tryptophan. This method would seem to be general across the superfamily.

-	~~<!--~~	+	Structural insights into regioselectivity in the enzymatic chlorination of tryptophan.,Zhu X, De Laurentis W, Leang K, Herrmann J, Ihlefeld K, van Pee KH, Naismith JH J Mol Biol. 2009 Aug 7;391(1):74-85. Epub 2009 Jun 6. PMID:19501593<ref>PMID:19501593</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2wes"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_2wes\| PDB=2wes \| SCENE= }}~~	+

-	~~===CRYSTAL STRUCTURES OF MUTANT E46Q OF TRYPTOPHAN 5-HALOGENASE (PYRH)===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_19501593}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 19501593 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_19501593}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~2WES is a 4 chains structure of sequences from [http:~~/~~/en.wikipedia.org/wiki/Streptomyces_rugosporus Streptomyces rugosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:19501593<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces rugosporus]]		[[Category: Streptomyces rugosporus]]
	[[Category: Naismith, J H.]]		[[Category: Naismith, J H.]]
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	[[Category: Regioselectivity]]		[[Category: Regioselectivity]]
	[[Category: Tryptophan 5-halogenase]]		[[Category: Tryptophan 5-halogenase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 09:12:17 2009''

OCA at 06:12, 25 June 2009

2009-06-25T06:12:19Z

←Older revision		Revision as of 06:12, 25 June 2009
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	===CRYSTAL STRUCTURES OF MUTANT E46Q OF TRYPTOPHAN 5-HALOGENASE (PYRH)===		===CRYSTAL STRUCTURES OF MUTANT E46Q OF TRYPTOPHAN 5-HALOGENASE (PYRH)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19501593}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19501593 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19501593}}

	==About this Structure==		==About this Structure==
	2WES is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_rugosporus Streptomyces rugosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA].		2WES is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_rugosporus Streptomyces rugosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WES OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19501593</ref><references group="xtra"/>
	[[Category: Streptomyces rugosporus]]		[[Category: Streptomyces rugosporus]]
	[[Category: Naismith, J H.]]		[[Category: Naismith, J H.]]
Line 22:		Line 31:
	[[Category: Tryptophan 5-halogenase]]		[[Category: Tryptophan 5-halogenase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jun ~~24 20~~:21:58 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 09:12:17 2009''