2wk1 - Revision history

OCA at 15:59, 13 December 2023

2023-12-13T15:59:44Z

←Older revision		Revision as of 15:59, 13 December 2023
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	<StructureSection load='2wk1' size='340' side='right'caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2wk1' size='340' side='right'caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinoalloteichus_caeruleus Actinoalloteichus caeruleus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [https://pdbe.org/2wk1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [https://www.ebi.ac.uk/pdbsum/2wk1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wk1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [https://pdbe.org/2wk1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [https://www.ebi.ac.uk/pdbsum/2wk1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wk1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NOVP_STRNV NOVP_STRNV] S-adenosyl-L-methionine-dependent O-methyltransferase that methylates at 4-OH of the noviose moiety, the penultimate step in the novobiocin biosynthesis pathway. Novobiocin is an aminocoumarin family antibiotic that targets bacterial DNA gyrases.<ref>PMID:14694473</ref> <ref>PMID:15218104</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actinoalloteichus caeruleus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Garcia, I Gomez~~]]	+	[[Category: Freel Meyers CL]]
-	[[Category: ~~Lawson, D M~~]]	+	[[Category: Gomez Garcia I]]
-	[[Category: ~~Meyers, C L.Freel~~]]	+	[[Category: Lawson DM]]
-	[[Category: Stevenson~~, C E.M~~]]	+	[[Category: Stevenson CEM]]
-	[[Category: Uson, I]]	+	[[Category: Uson I]]
-	[[Category: Walsh~~, C T]]~~	+	[[Category: Walsh CT]]
-	~~[[Category: Novobiocin]]~~	+
-	~~[[Category: Novp]]~~	+
-	~~[[Category: O-methyltransferase]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Tylf superfamily~~]]	+

OCA at 11:09, 6 April 2022

2022-04-06T11:09:28Z

←Older revision		Revision as of 11:09, 6 April 2022
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	<StructureSection load='2wk1' size='340' side='right'caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2wk1' size='340' side='right'caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [~~http~~://pdbe.org/2wk1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wk1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wk1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [https://pdbe.org/2wk1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [https://www.ebi.ac.uk/pdbsum/2wk1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wk1 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:50, 1 July 2020

2020-07-01T08:50:31Z

←Older revision		Revision as of 08:50, 1 July 2020
Line 1:		Line 1:

	==Structure of the O-methyltransferase NovP==		==Structure of the O-methyltransferase NovP==
-	<StructureSection load='2wk1' size='340' side='right' caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='2wk1' size='340' side='right'caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [http://pdbe.org/2wk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wk1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [http://pdbe.org/2wk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wk1 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Garcia, I Gomez]]		[[Category: Garcia, I Gomez]]
	[[Category: Lawson, D M]]		[[Category: Lawson, D M]]

OCA at 09:23, 26 September 2018

2018-09-26T09:23:34Z

←Older revision		Revision as of 09:23, 26 September 2018
Line 1:		Line 1:
-	==~~STRUCTURE OF THE~~ O-~~METHYLTRANSFERASE NOVP~~==	+
		+	==Structure of the O-methyltransferase NovP==
	<StructureSection load='2wk1' size='340' side='right' caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2wk1' size='340' side='right' caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [http://pdbe.org/2wk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wk1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [http://pdbe.org/2wk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wk1 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wk1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wk1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:42, 9 February 2016

2016-02-09T20:42:48Z

←Older revision		Revision as of 20:42, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2wk1' size='340' side='right' caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2wk1' size='340' side='right' caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Streptomyces_caeruleus Streptomyces caeruleus]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wk1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [http://pdbe.org/2wk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wk1 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wk1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wk1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces caeruleus]]
	[[Category: Garcia, I Gomez]]		[[Category: Garcia, I Gomez]]
	[[Category: Lawson, D M]]		[[Category: Lawson, D M]]

OCA at 08:17, 20 January 2015

2015-01-20T08:17:31Z

←Older revision		Revision as of 08:17, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_caeruleus Streptomyces caeruleus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_caeruleus Streptomyces caeruleus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wk1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wk1 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces caeruleus]]		[[Category: Streptomyces caeruleus]]
-	[[Category: Garcia, I Gomez.]]	+	[[Category: Garcia, I Gomez]]
-	[[Category: Lawson, D M.]]	+	[[Category: Lawson, D M]]
-	[[Category: Meyers, C L.Freel.]]	+	[[Category: Meyers, C L.Freel]]
-	[[Category: Stevenson, C E.M.]]	+	[[Category: Stevenson, C E.M]]
-	[[Category: Uson, I.]]	+	[[Category: Uson, I]]
-	[[Category: Walsh, C T.]]	+	[[Category: Walsh, C T]]
	[[Category: Novobiocin]]		[[Category: Novobiocin]]
	[[Category: Novp]]		[[Category: Novp]]

OCA at 04:18, 3 October 2014

2014-10-03T04:18:14Z

←Older revision		Revision as of 04:18, 3 October 2014
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-	~~{{STRUCTURE_2wk1~~\| ~~PDB~~=2wk1 \| ~~SCENE~~= }}	+	==STRUCTURE OF THE O-METHYLTRANSFERASE NOVP==
-	===~~STRUCTURE OF THE O~~-~~METHYLTRANSFERASE NOVP~~===	+	<StructureSection load='2wk1' size='340' side='right' caption='[[2wk1]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19857499}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wk1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_caeruleus Streptomyces caeruleus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WK1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wk1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wk1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wk1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	NovP is an S-adenosyl-l-methionine-dependent O-methyltransferase that catalyzes the penultimate step in the biosynthesis of the aminocoumarin antibiotic novobiocin. Specifically, it methylates at 4-OH of the noviose moiety, and the resultant methoxy group is important for the potency of the mature antibiotic: previous crystallographic studies have shown that this group interacts directly with the target enzyme DNA gyrase, which is a validated drug target. We have determined the high-resolution crystal structure of NovP from Streptomyces spheroides as a binary complex with its desmethylated cosubstrate S-adenosyl-l-homocysteine. The structure displays a typical class I methyltransferase fold, in addition to motifs that are consistent with a divalent-metal-dependent mechanism. This is the first representative structure of a methyltransferase from the TylF superfamily, which includes a number of enzymes implicated in the biosynthesis of antibiotics and other therapeutics. The NovP structure reveals a number of distinctive structural features that, based on sequence conservation, are likely to be characteristic of the superfamily. These include a helical 'lid' region that gates access to the cosubstrate binding pocket and an active center that contains a 3-Asp putative metal binding site. A further conserved Asp likely acts as the general base that initiates the reaction by deprotonating the 4-OH group of the noviose unit. Using in silico docking, we have generated models of the enzyme-substrate complex that are consistent with the proposed mechanism. Furthermore, these models suggest that NovP is unlikely to tolerate significant modifications at the noviose moiety, but could show increasing substrate promiscuity as a function of the distance of the modification from the methylation site. These observations could inform future attempts to utilize NovP for methylating a range of glycosylated compounds.

-	~~==About this Structure==~~	+	The crystal structure of the novobiocin biosynthetic enzyme NovP: the first representative structure for the TylF O-methyltransferase superfamily.,Gomez Garcia I, Stevenson CE, Uson I, Freel Meyers CL, Walsh CT, Lawson DM J Mol Biol. 2010 Jan 15;395(2):390-407. Epub 2009 Oct 24. PMID:19857499<ref>PMID:19857499</ref>
-	~~[[2wk1]] is a 1 chain~~ structure ~~with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Streptomyces_caeruleus Streptomyces caeruleus]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2WK1 OCA].~~	+

-	~~==Reference==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:019857499<~~/~~ref>~~<~~ref group="xtra"~~>~~PMID:017329822~~</~~ref~~>~~<ref group~~=~~"xtra">PMID:017881824</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces caeruleus]]		[[Category: Streptomyces caeruleus]]
	[[Category: Garcia, I Gomez.]]		[[Category: Garcia, I Gomez.]]

OCA at 08:09, 8 May 2013

2013-05-08T08:09:08Z

←Older revision		Revision as of 08:09, 8 May 2013
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-	[[Image:2wk1.png\|left\|200px]]
-
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	{{STRUCTURE_2wk1\| PDB=2wk1 \| SCENE= }}		{{STRUCTURE_2wk1\| PDB=2wk1 \| SCENE= }}
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	===STRUCTURE OF THE O-METHYLTRANSFERASE NOVP===		===STRUCTURE OF THE O-METHYLTRANSFERASE NOVP===
-
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-	<!--
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	{{ABSTRACT_PUBMED_19857499}}		{{ABSTRACT_PUBMED_19857499}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:019857499</ref><ref group="xtra">PMID:017329822</ref><ref group="xtra">PMID:017881824</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019857499</ref><ref group="xtra">PMID:017329822</ref><ref group="xtra">PMID:017881824</ref><references group="xtra"/><references/>
	[[Category: Streptomyces caeruleus]]		[[Category: Streptomyces caeruleus]]
	[[Category: Garcia, I Gomez.]]		[[Category: Garcia, I Gomez.]]

OCA at 17:56, 6 July 2011

2011-07-06T17:56:40Z

←Older revision		Revision as of 17:56, 6 July 2011
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19857499~~</ref><ref group="xtra">PMID:~~17329822~~</ref><ref group="xtra">PMID:~~17881824~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019857499</ref><ref group="xtra">PMID:017329822</ref><ref group="xtra">PMID:017881824</ref><references group="xtra"/>
	[[Category: Streptomyces caeruleus]]		[[Category: Streptomyces caeruleus]]
	[[Category: Garcia, I Gomez.]]		[[Category: Garcia, I Gomez.]]

OCA: Protected "2wk1" [edit=sysop:move=sysop]

2011-03-14T18:45:42Z

Protected "2wk1" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:45, 14 March 2011