2wkq - Revision history

OCA at 16:00, 13 December 2023

2023-12-13T16:00:20Z

←Older revision		Revision as of 16:00, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2wkq' size='340' side='right'caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2wkq' size='340' side='right'caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Avesa Avesa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Avena_sativa Avena sativa] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkq OCA], [https://pdbe.org/2wkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wkq RCSB], [https://www.ebi.ac.uk/pdbsum/2wkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkq OCA], [https://pdbe.org/2wkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wkq RCSB], [https://www.ebi.ac.uk/pdbsum/2wkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RAC1_HUMAN RAC1_HUMAN] Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref> Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref> [https://www.uniprot.org/uniprot/O49003_AVESA O49003_AVESA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Avesa~~]]	+	[[Category: Avena sativa]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Frey, D]]	+	[[Category: Frey D]]
-	[[Category: Hahn~~, K M~~]]	+	[[Category: Hahn KM]]
-	[[Category: Jaehrig, A]]	+	[[Category: Jaehrig A]]
-	[[Category: Kuhlman, B]]	+	[[Category: Kuhlman B]]
-	[[Category: Lungu~~, O I~~]]	+	[[Category: Lungu OI]]
-	[[Category: Schlichting, I]]	+	[[Category: Schlichting I]]
-	[[Category: Wu~~, Y I]]~~	+	[[Category: Wu YI]]
-	~~[[Category: Adp-ribosylation]]~~	+
-	~~[[Category: Alternative splicing]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Cell membrane]]~~	+
-	~~[[Category: Chimera]]~~	+
-	~~[[Category: Gtp-binding]]~~	+
-	~~[[Category: Gtpase]]~~	+
-	~~[[Category: Light- induced signal transduction]]~~	+
-	~~[[Category: Light-induced signal transduction]]~~	+
-	~~[[Category: Lipoprotein]]~~	+
-	~~[[Category: Lov2]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Nucleotide-binding protein engineering]]~~	+
-	~~[[Category: Phototropin1]]~~	+
-	~~[[Category: Protein design]]~~	+
-	~~[[Category: Protein engineering]]~~	+
-	~~[[Category: Ras superfamily lov2]]~~	+
-	~~[[Category: Rho family]]~~	+
-	~~[[Category: Small g-protein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:45, 29 June 2022

2022-06-29T07:45:17Z

←Older revision		Revision as of 07:45, 29 June 2022
Line 3:		Line 3:
	<StructureSection load='2wkq' size='340' side='right'caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2wkq' size='340' side='right'caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Avesa Avesa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Avesa Avesa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkq OCA], [~~http~~://pdbe.org/2wkq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wkq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wkq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkq OCA], [https://pdbe.org/2wkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wkq RCSB], [https://www.ebi.ac.uk/pdbsum/2wkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:50, 23 September 2020

2020-09-23T11:50:22Z

←Older revision		Revision as of 11:50, 23 September 2020
Line 1:		Line 1:

	==Structure of a photoactivatable Rac1 containing the Lov2 C450A Mutant==		==Structure of a photoactivatable Rac1 containing the Lov2 C450A Mutant==
-	<StructureSection load='2wkq' size='340' side='right' caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='2wkq' size='340' side='right'caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Avesa Avesa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Avesa Avesa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkq OCA], [http://pdbe.org/2wkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wkq RCSB], [http://www.ebi.ac.uk/pdbsum/2wkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkq OCA], [http://pdbe.org/2wkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wkq RCSB], [http://www.ebi.ac.uk/pdbsum/2wkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[Rac\|Rac]]	+	*[[Rac 3D structures\|Rac 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Avesa]]		[[Category: Avesa]]
		+	[[Category: Large Structures]]
	[[Category: Frey, D]]		[[Category: Frey, D]]
	[[Category: Hahn, K M]]		[[Category: Hahn, K M]]

OCA at 09:32, 19 December 2018

2018-12-19T09:32:11Z

←Older revision		Revision as of 09:32, 19 December 2018
Line 3:		Line 3:
	<StructureSection load='2wkq' size='340' side='right' caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2wkq' size='340' side='right' caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Avesa Avesa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Avesa]]
	[[Category: Frey, D]]		[[Category: Frey, D]]
	[[Category: Hahn, K M]]		[[Category: Hahn, K M]]

OCA at 13:19, 15 March 2017

2017-03-15T13:19:27Z

←Older revision		Revision as of 13:19, 15 March 2017
Line 1:		Line 1:

-	==~~STRUCTURE OF A PHOTOACTIVATABLE RAC1 CONTAINING THE LOV2~~ C450A ~~MUTANT~~==	+	==Structure of a photoactivatable Rac1 containing the Lov2 C450A Mutant==
	<StructureSection load='2wkq' size='340' side='right' caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2wkq' size='340' side='right' caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Avesa Avesa]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 2wkq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2wkq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Rac\|Rac]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Avesa]]
	[[Category: Frey, D]]		[[Category: Frey, D]]
	[[Category: Hahn, K M]]		[[Category: Hahn, K M]]
Line 30:		Line 32:
	[[Category: Wu, Y I]]		[[Category: Wu, Y I]]
	[[Category: Adp-ribosylation]]		[[Category: Adp-ribosylation]]
		+	[[Category: Alternative splicing]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]

OCA at 14:23, 11 August 2016

2016-08-11T14:23:15Z

←Older revision		Revision as of 14:23, 11 August 2016
Line 1:		Line 1:
		+
	==STRUCTURE OF A PHOTOACTIVATABLE RAC1 CONTAINING THE LOV2 C450A MUTANT==		==STRUCTURE OF A PHOTOACTIVATABLE RAC1 CONTAINING THE LOV2 C450A MUTANT==
	<StructureSection load='2wkq' size='340' side='right' caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2wkq' size='340' side='right' caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Avena_sativa Avena sativa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Avesa Avesa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wkq RCSB], [http://www.ebi.ac.uk/pdbsum/2wkq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkq OCA], [http://pdbe.org/2wkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wkq RCSB], [http://www.ebi.ac.uk/pdbsum/2wkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wkq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Avena sativa~~]]	+	[[Category: Avesa]]
	[[Category: Frey, D]]		[[Category: Frey, D]]
	[[Category: Hahn, K M]]		[[Category: Hahn, K M]]

OCA at 06:39, 22 January 2015

2015-01-22T06:39:15Z

←Older revision		Revision as of 06:39, 22 January 2015
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Avena sativa]]		[[Category: Avena sativa]]
-	[[Category: Frey, D.]]	+	[[Category: Frey, D]]
-	[[Category: Hahn, K M.]]	+	[[Category: Hahn, K M]]
-	[[Category: Jaehrig, A.]]	+	[[Category: Jaehrig, A]]
-	[[Category: Kuhlman, B.]]	+	[[Category: Kuhlman, B]]
-	[[Category: Lungu, O I.]]	+	[[Category: Lungu, O I]]
-	[[Category: Schlichting, I.]]	+	[[Category: Schlichting, I]]
-	[[Category: Wu, Y I.]]	+	[[Category: Wu, Y I]]
	[[Category: Adp-ribosylation]]		[[Category: Adp-ribosylation]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA at 11:27, 20 October 2014

2014-10-20T11:27:10Z

←Older revision		Revision as of 11:27, 20 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2wkq.~~png~~\|~~left~~\|~~200px~~]]	+	==STRUCTURE OF A PHOTOACTIVATABLE RAC1 CONTAINING THE LOV2 C450A MUTANT==
		+	<StructureSection load='2wkq' size='340' side='right' caption='[[2wkq]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Avena_sativa Avena sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2v0u\|2v0u]], [[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[2v0w\|2v0w]], [[1ryf\|1ryf]], [[2v1a\|2v1a]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[1g4u\|1g4u]], [[2fju\|2fju]], [[1i4t\|1i4t]], [[2v1b\|2v1b]], [[2wkr\|2wkr]], [[2wkp\|2wkp]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wkq RCSB], [http://www.ebi.ac.uk/pdbsum/2wkq PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The precise spatio-temporal dynamics of protein activity are often critical in determining cell behaviour, yet for most proteins they remain poorly understood; it remains difficult to manipulate protein activity at precise times and places within living cells. Protein activity has been controlled by light, through protein derivatization with photocleavable moieties or using photoreactive small-molecule ligands. However, this requires use of toxic ultraviolet wavelengths, activation is irreversible, and/or cell loading is accomplished via disruption of the cell membrane (for example, through microinjection). Here we have developed a new approach to produce genetically encoded photoactivatable derivatives of Rac1, a key GTPase regulating actin cytoskeletal dynamics in metazoan cells. Rac1 mutants were fused to the photoreactive LOV (light oxygen voltage) domain from phototropin, sterically blocking Rac1 interactions until irradiation unwound a helix linking LOV to Rac1. Photoactivatable Rac1 (PA-Rac1) could be reversibly and repeatedly activated using 458- or 473-nm light to generate precisely localized cell protrusions and ruffling. Localized Rac activation or inactivation was sufficient to produce cell motility and control the direction of cell movement. Myosin was involved in Rac control of directionality but not in Rac-induced protrusion, whereas PAK was required for Rac-induced protrusion. PA-Rac1 was used to elucidate Rac regulation of RhoA in cell motility. Rac and Rho coordinate cytoskeletal behaviours with seconds and submicrometre precision. Their mutual regulation remains controversial, with data indicating that Rac inhibits and/or activates Rho. Rac was shown to inhibit RhoA in mouse embryonic fibroblasts, with inhibition modulated at protrusions and ruffles. A PA-Rac crystal structure and modelling revealed LOV-Rac interactions that will facilitate extension of this photoactivation approach to other proteins.

-	~~{{STRUCTURE_2wkq\| PDB=2wkq \| SCENE= }}~~	+	A genetically encoded photoactivatable Rac controls the motility of living cells.,Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM Nature. 2009 Sep 3;461(7260):104-8. Epub 2009 Aug 19. PMID:19693014<ref>PMID:19693014</ref>

-	~~===STRUCTURE OF A PHOTOACTIVATABLE RAC1 CONTAINING THE LOV2 C450A MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19693014}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2wkq]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Avena_sativa Avena sativa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019693014<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Avena sativa]]		[[Category: Avena sativa]]
	[[Category: Frey, D.]]		[[Category: Frey, D.]]

OCA: Protected "2wkq" [edit=sysop:move=sysop]

2013-01-07T17:54:52Z

Protected "2wkq" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:54, 7 January 2013

OCA at 17:54, 7 January 2013

2013-01-07T17:54:49Z

←Older revision		Revision as of 17:54, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2wkq.png\|left\|200px]]		[[Image:2wkq.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2wkq", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2wkq\| PDB=2wkq \| SCENE= }}		{{STRUCTURE_2wkq\| PDB=2wkq \| SCENE= }}

	===STRUCTURE OF A PHOTOACTIVATABLE RAC1 CONTAINING THE LOV2 C450A MUTANT===		===STRUCTURE OF A PHOTOACTIVATABLE RAC1 CONTAINING THE LOV2 C450A MUTANT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19693014}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19693014 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19693014}}		{{ABSTRACT_PUBMED_19693014}}

	==About this Structure==		==About this Structure==
-	~~2WKQ~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Avena_sativa~~,_homo_sapiens~~ Avena sativa~~, homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA].	+	[[2wkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Avena_sativa Avena sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKQ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19693014~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019693014</ref><references group="xtra"/>
-	[[Category: Avena sativa~~, homo sapiens~~]]	+	[[Category: Avena sativa]]
	[[Category: Frey, D.]]		[[Category: Frey, D.]]
	[[Category: Hahn, K M.]]		[[Category: Hahn, K M.]]
Line 33:		Line 21:
	[[Category: Wu, Y I.]]		[[Category: Wu, Y I.]]
	[[Category: Adp-ribosylation]]		[[Category: Adp-ribosylation]]
-	[[Category: Alternative splicing]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
Line 53:		Line 40:
	[[Category: Small g-protein]]		[[Category: Small g-protein]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 15:34:01 2009''