2wqq - Revision history

OCA at 10:14, 20 December 2023

2023-12-20T10:14:11Z

←Older revision		Revision as of 10:14, 20 December 2023
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	<StructureSection load='2wqq' size='340' side='right'caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2wqq' size='340' side='right'caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp.~~ jejuni~~" smibert 1974~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WQQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WQQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CSF:CYTIDINE~~-~~5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>~~, ~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1ro8\|1ro8]]~~, ~~[[1ro7\|1ro7]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [https://pdbe.org/2wqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [https://www.ebi.ac.uk/pdbsum/2wqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wqq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [https://pdbe.org/2wqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [https://www.ebi.ac.uk/pdbsum/2wqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wqq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9LAK3_CAMJU Q9LAK3_CAMJU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Campylobacter ~~fetus subsp.~~ jejuni ~~smibert 1974~~]]	+	[[Category: Campylobacter jejuni]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chan~~, P H.W~~]]	+	[[Category: Chan PHW]]
-	[[Category: Lairson~~, L L~~]]	+	[[Category: Lairson LL]]
-	[[Category: Lee~~, H J~~]]	+	[[Category: Lee HJ]]
-	[[Category: McIntosh~~, L P~~]]	+	[[Category: McIntosh LP]]
-	[[Category: Strynadka~~, N C.J~~]]	+	[[Category: Strynadka NCJ]]
-	[[Category: Wakarchuk~~, W W~~]]	+	[[Category: Wakarchuk WW]]
-	[[Category: Withers~~, S G]]~~	+	[[Category: Withers SG]]
-	~~[[Category: Cstii]]~~	+
-	~~[[Category: Glycosyltransferase]]~~	+
-	~~[[Category: Gta]]~~	+
-	~~[[Category: Sialyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:13, 6 April 2022

2022-04-06T11:13:47Z

←Older revision		Revision as of 11:13, 6 April 2022
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	<StructureSection load='2wqq' size='340' side='right'caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2wqq' size='340' side='right'caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WQQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WQQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ro8\|1ro8]], [[1ro7\|1ro7]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [~~http~~://pdbe.org/2wqq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wqq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wqq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [https://pdbe.org/2wqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [https://www.ebi.ac.uk/pdbsum/2wqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wqq ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 09:04, 1 July 2020

2020-07-01T09:04:47Z

←Older revision		Revision as of 09:04, 1 July 2020
Line 1:		Line 1:

	==Crystallographic analysis of monomeric CstII==		==Crystallographic analysis of monomeric CstII==
-	<StructureSection load='2wqq' size='340' side='right' caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='2wqq' size='340' side='right'caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WQQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WQQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [http://pdbe.org/2wqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [http://www.ebi.ac.uk/pdbsum/2wqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wqq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [http://pdbe.org/2wqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [http://www.ebi.ac.uk/pdbsum/2wqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wqq ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Sialyltransferase\|Sialyltransferase]]	+	*[[Sialyltransferase 3D structures\|Sialyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]		[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
		+	[[Category: Large Structures]]
	[[Category: Chan, P H.W]]		[[Category: Chan, P H.W]]
	[[Category: Lairson, L L]]		[[Category: Lairson, L L]]

OCA at 09:04, 26 September 2018

2018-09-26T09:04:16Z

←Older revision		Revision as of 09:04, 26 September 2018
Line 1:		Line 1:
		+
	==Crystallographic analysis of monomeric CstII==		==Crystallographic analysis of monomeric CstII==
	<StructureSection load='2wqq' size='340' side='right' caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2wqq' size='340' side='right' caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [http://pdbe.org/2wqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [http://www.ebi.ac.uk/pdbsum/2wqq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [http://pdbe.org/2wqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [http://www.ebi.ac.uk/pdbsum/2wqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wqq ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wq/2wqq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wq/2wqq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 2wqq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2wqq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Sialyltransferase\|Sialyltransferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:40, 8 February 2016

2016-02-08T05:40:52Z

←Older revision		Revision as of 05:40, 8 February 2016
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [http://www.ebi.ac.uk/pdbsum/2wqq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [http://pdbe.org/2wqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [http://www.ebi.ac.uk/pdbsum/2wqq PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wqq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wqq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:27, 1 April 2015

2015-04-01T07:27:42Z

←Older revision		Revision as of 07:27, 1 April 2015
Line 1:		Line 1:
-	==~~CRYSTALLOGRAPHIC ANALYSIS OF MONOMERIC CSTII~~==	+	==Crystallographic analysis of monomeric CstII==
	<StructureSection load='2wqq' size='340' side='right' caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2wqq' size='340' side='right' caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Campylobacter_jejuni Campylobacter~~ jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WQQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WQQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>
Line 29:		Line 29:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Campylobacter jejuni]]	+	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
	[[Category: Chan, P H.W]]		[[Category: Chan, P H.W]]
	[[Category: Lairson, L L]]		[[Category: Lairson, L L]]
	[[Category: Lee, H J]]		[[Category: Lee, H J]]
-	[[Category: ~~Mcintosh~~, L P]]	+	[[Category: McIntosh, L P]]
	[[Category: Strynadka, N C.J]]		[[Category: Strynadka, N C.J]]
	[[Category: Wakarchuk, W W]]		[[Category: Wakarchuk, W W]]

OCA at 10:09, 20 January 2015

2015-01-20T10:09:26Z

←Older revision		Revision as of 10:09, 20 January 2015
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Campylobacter jejuni]]		[[Category: Campylobacter jejuni]]
-	[[Category: Chan, P H.W.]]	+	[[Category: Chan, P H.W]]
-	[[Category: Lairson, L L.]]	+	[[Category: Lairson, L L]]
-	[[Category: Lee, H J.]]	+	[[Category: Lee, H J]]
-	[[Category: Mcintosh, L P.]]	+	[[Category: Mcintosh, L P]]
-	[[Category: Strynadka, N C.J.]]	+	[[Category: Strynadka, N C.J]]
-	[[Category: Wakarchuk, W W.]]	+	[[Category: Wakarchuk, W W]]
-	[[Category: Withers, S G.]]	+	[[Category: Withers, S G]]
	[[Category: Cstii]]		[[Category: Cstii]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]

OCA at 05:55, 10 October 2014

2014-10-10T05:55:28Z

←Older revision		Revision as of 05:55, 10 October 2014
Line 2:		Line 2:
	<StructureSection load='2wqq' size='340' side='right' caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2wqq' size='340' side='right' caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. <br>	+	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WQQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>
-	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [http://www.ebi.ac.uk/pdbsum/2wqq PDBsum]</span></td></tr>
-	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [http://www.ebi.ac.uk/pdbsum/2wqq PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 23:
	NMR Spectroscopic Characterization of the Sialyltransferase CstII from Camplyobacter jejuni: Histidine 188 is the General Base.,Chan PH, Lairson LL, Lee HJ, Wakarchuk WW, Strynadka NC, Withers SG, McIntosh LP Biochemistry. 2009 Oct 13. PMID:19824695<ref>PMID:19824695</ref>		NMR Spectroscopic Characterization of the Sialyltransferase CstII from Camplyobacter jejuni: Histidine 188 is the General Base.,Chan PH, Lairson LL, Lee HJ, Wakarchuk WW, Strynadka NC, Withers SG, McIntosh LP Biochemistry. 2009 Oct 13. PMID:19824695<ref>PMID:19824695</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 08:37, 7 May 2014

2014-05-07T08:37:45Z

←Older revision		Revision as of 08:37, 7 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2wqq.~~png~~\|~~left~~\|200px]]	+	==CRYSTALLOGRAPHIC ANALYSIS OF MONOMERIC CSTII==
		+	<StructureSection load='2wqq' size='340' side='right' caption='[[2wqq]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wqq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ro8\|1ro8]], [[1ro7\|1ro7]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wqq RCSB], [http://www.ebi.ac.uk/pdbsum/2wqq PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wq/2wqq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cell surface glycans are often terminated by sialic acid, which is incorporated onto sugar acceptors by sialyltransferases. The crystal structure of the GT family 42 Campylobacter jejuni alpha-2,3/2,8-sialyltransferase (CstII) provides key insights into the sialyl-transfer mechanism, including tentative identification of His188 as the catalytic base. In support of this hypothesis, the CstII-H188A mutant is able to catalyze sialyl transfer from CMP-Neu5Ac to added anions such as azide and formate, but not to its natural sugar acceptor lactose. Complementing this work, NMR spectroscopy was used to investigate the structure and dynamics of CstII and to measure the intrinsic pKa value of His188 for comparison with the pKa determined from the pH-dependent kcat/KM of enzyme. By systematically introducing point mutations at the subunit interfaces, two active monomeric variants, CstII-F121D and CstII-Y125Q, were obtained and characterized. In contrast to the wild-type tetramer, the monomeric CstII variants yielded good quality 1H/15N-HSQC and 1H/13C methyl-TROSY NMR spectra. However, the absence of signals from approximately one half of the amides in the 1H/15N-HSQC spectra of both monomeric forms suggests that the enzyme undergoes substantial conformational motions on a msec-musec timescale. The histidine pKa values of CstII-F121D in its apo form were measured by monitoring the pH-dependent chemical shifts of [13Cepsilon1]-histidine, biosynthetically incorporated into the otherwise uniformly deuterated protein. Consistent with its proposed catalytic role, the site-specific pKa value ~ 6.6 of His188 matches the apparent pKa value ~ 6.5 governing the pH-dependence of kcat/KM for CstII towards CMP-Neu5Ac in the presence of saturating acceptor substrate.

-	~~<!--~~	+	NMR Spectroscopic Characterization of the Sialyltransferase CstII from Camplyobacter jejuni: Histidine 188 is the General Base.,Chan PH, Lairson LL, Lee HJ, Wakarchuk WW, Strynadka NC, Withers SG, McIntosh LP Biochemistry. 2009 Oct 13. PMID:19824695<ref>PMID:19824695</ref>
-	~~The line below this paragraph, containing "STRUCTURE_2wqq", creates~~ the ~~"Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_2wqq\| PDB=2wqq \| SCENE= }}~~	+

-	~~===CRYSTALLOGRAPHIC ANALYSIS OF MONOMERIC CSTII===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_19824695}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 19824695 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_19824695}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2wqq]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQQ OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019824695<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Campylobacter jejuni]]		[[Category: Campylobacter jejuni]]
	[[Category: Chan, P H.W.]]		[[Category: Chan, P H.W.]]

OCA: Protected "2wqq" [edit=sysop:move=sysop]

2011-11-02T07:43:54Z

Protected "2wqq" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:43, 2 November 2011