2wsx - Revision history

OCA at 10:16, 9 May 2024

2024-05-09T10:16:45Z

←Older revision		Revision as of 10:16, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wsx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WSX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wsx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WSX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NM2:3~~-~~CARBOXY-N~~,~~N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2wsw\|2wsw]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [https://pdbe.org/2wsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [https://www.ebi.ac.uk/pdbsum/2wsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [https://pdbe.org/2wsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [https://www.ebi.ac.uk/pdbsum/2wsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CAIT_ECOLI CAIT_ECOLI]] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines.	+	[https://www.uniprot.org/uniprot/CAIT_ECOLI CAIT_ECOLI] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kuehlbrandt, W]]	+	[[Category: Kuehlbrandt W]]
-	~~[[Category: Scheltinga, A C.Terwisscha van~~]]	+	[[Category: Schulze S]]
-	[[Category: Schulze, S]]	+	[[Category: Terwisscha van Scheltinga AC]]
-	[[Category: ~~Membrane protein]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 11:15, 6 April 2022

2022-04-06T11:15:21Z

←Older revision		Revision as of 11:15, 6 April 2022
Line 3:		Line 3:
	<StructureSection load='2wsx' size='340' side='right'caption='[[2wsx]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='2wsx' size='340' side='right'caption='[[2wsx]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wsx]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WSX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wsx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WSX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wsw\|2wsw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wsw\|2wsw]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [~~http~~://pdbe.org/2wsx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wsx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [https://pdbe.org/2wsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [https://www.ebi.ac.uk/pdbsum/2wsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CAIT_ECOLI CAIT_ECOLI]] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines.	+	[[https://www.uniprot.org/uniprot/CAIT_ECOLI CAIT_ECOLI]] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:08, 1 July 2020

2020-07-01T09:08:30Z

←Older revision		Revision as of 09:08, 1 July 2020
Line 1:		Line 1:

	==Crystal Structure of Carnitine Transporter from Escherichia coli==		==Crystal Structure of Carnitine Transporter from Escherichia coli==
-	<StructureSection load='2wsx' size='340' side='right' caption='[[2wsx]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='2wsx' size='340' side='right'caption='[[2wsx]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wsx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WSX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wsx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WSX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wsw\|2wsw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wsw\|2wsw]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [http://pdbe.org/2wsx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [http://www.ebi.ac.uk/pdbsum/2wsx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [http://pdbe.org/2wsx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [http://www.ebi.ac.uk/pdbsum/2wsx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
	[[Category: Kuehlbrandt, W]]		[[Category: Kuehlbrandt, W]]
	[[Category: Scheltinga, A C.Terwisscha van]]		[[Category: Scheltinga, A C.Terwisscha van]]

OCA at 08:56, 26 September 2018

2018-09-26T08:56:49Z

←Older revision		Revision as of 08:56, 26 September 2018
Line 1:		Line 1:

-	==~~CRYSTAL STRUCTURE OF CARNITINE TRANSPORTER FROM ESCHERICHIA COLI~~==	+	==Crystal Structure of Carnitine Transporter from Escherichia coli==
	<StructureSection load='2wsx' size='340' side='right' caption='[[2wsx]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='2wsx' size='340' side='right' caption='[[2wsx]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/2wsx_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/2wsx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Kuehlbrandt, W]]		[[Category: Kuehlbrandt, W]]
-	[[Category: Scheltinga, A C.Terwisscha ~~Van~~]]	+	[[Category: Scheltinga, A C.Terwisscha van]]
	[[Category: Schulze, S]]		[[Category: Schulze, S]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 05:22, 5 August 2016

2016-08-05T05:22:53Z

←Older revision		Revision as of 05:22, 5 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF CARNITINE TRANSPORTER FROM ESCHERICHIA COLI==		==CRYSTAL STRUCTURE OF CARNITINE TRANSPORTER FROM ESCHERICHIA COLI==
	<StructureSection load='2wsx' size='340' side='right' caption='[[2wsx]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='2wsx' size='340' side='right' caption='[[2wsx]], [[Resolution\|resolution]] 3.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wsw\|2wsw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wsw\|2wsw]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [http://www.ebi.ac.uk/pdbsum/2wsx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [http://pdbe.org/2wsx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [http://www.ebi.ac.uk/pdbsum/2wsx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wsx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wsx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:21, 24 December 2014

2014-12-24T23:21:10Z

←Older revision		Revision as of 23:21, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [http://www.ebi.ac.uk/pdbsum/2wsx PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [http://www.ebi.ac.uk/pdbsum/2wsx PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAIT_ECOLI CAIT_ECOLI]] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:51, 17 December 2014

2014-12-17T13:51:06Z

←Older revision		Revision as of 13:51, 17 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2wsx\| PDB=2wsx \| SCENE= }}~~	+	==CRYSTAL STRUCTURE OF CARNITINE TRANSPORTER FROM ESCHERICHIA COLI==
-	===CRYSTAL STRUCTURE OF CARNITINE TRANSPORTER FROM ESCHERICHIA COLI===	+	<StructureSection load='2wsx' size='340' side='right' caption='[[2wsx]], [[Resolution\|resolution]] 3.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20829798}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wsx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WSX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wsw\|2wsw]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wsx RCSB], [http://www.ebi.ac.uk/pdbsum/2wsx PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/2wsx_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transport of solutes across biological membranes is performed by specialized secondary transport proteins in the lipid bilayer, and is essential for life. Here we report the structures of the sodium-independent carnitine/butyrobetaine antiporter CaiT from Proteus mirabilis (PmCaiT) at 2.3-A and from Escherichia coli (EcCaiT) at 3.5-A resolution. CaiT belongs to the family of betaine/carnitine/choline transporters (BCCT), which are mostly Na(+) or H(+) dependent, whereas EcCaiT is Na(+) and H(+) independent. The three-dimensional architecture of CaiT resembles that of the Na(+)-dependent transporters LeuT and BetP, but in CaiT a methionine sulphur takes the place of the Na(+) ion to coordinate the substrate in the central transport site, accounting for Na(+)-independent transport. Both CaiT structures show the fully open, inward-facing conformation, and thus complete the set of functional states that describe the alternating access mechanism. EcCaiT contains two bound butyrobetaine substrate molecules, one in the central transport site, the other in an extracellular binding pocket. In the structure of PmCaiT, a tryptophan side chain occupies the transport site, and access to the extracellular site is blocked. Binding of both substrates to CaiT reconstituted into proteoliposomes is cooperative, with Hill coefficients up to 1.7, indicating that the extracellular site is regulatory. We propose a mechanism whereby the occupied regulatory site increases the binding affinity of the transport site and initiates substrate translocation.

-	~~==About this Structure==~~	+	Structural basis of Na(+)-independent and cooperative substrate/product antiport in CaiT.,Schulze S, Koster S, Geldmacher U, Terwisscha van Scheltinga AC, Kuhlbrandt W Nature. 2010 Sep 9;467(7312):233-6. PMID:20829798<ref>PMID:20829798</ref>
-	~~[[2wsx]] is a 3 chain structure with sequence from [http:~~/~~/en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	<~~ref group="xtra">PMID:020829798<~~/~~ref~~><~~references group="xtra"~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Kuehlbrandt, W.]]	+	[[Category: Kuehlbrandt, W]]
-	[[Category: Scheltinga, A C.Terwisscha Van.]]	+	[[Category: Scheltinga, A C.Terwisscha Van]]
-	[[Category: Schulze, S.]]	+	[[Category: Schulze, S]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 10:44, 13 March 2013

2013-03-13T10:44:47Z

←Older revision		Revision as of 10:44, 13 March 2013
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-	[[Image:2wsx.png\|left\|200px]]
-
-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2wsx\| PDB=2wsx \| SCENE= }}		{{STRUCTURE_2wsx\| PDB=2wsx \| SCENE= }}
-
	===CRYSTAL STRUCTURE OF CARNITINE TRANSPORTER FROM ESCHERICHIA COLI===		===CRYSTAL STRUCTURE OF CARNITINE TRANSPORTER FROM ESCHERICHIA COLI===
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-
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	[[Category: Scheltinga, A C.Terwisscha Van.]]		[[Category: Scheltinga, A C.Terwisscha Van.]]
	[[Category: Schulze, S.]]		[[Category: Schulze, S.]]
		+	[[Category: Membrane protein]]
		+	[[Category: Transport protein]]

OCA: Protected "2wsx" [edit=sysop:move=sysop]

2011-05-12T04:05:52Z

Protected "2wsx" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 12 May 2011

OCA at 04:05, 12 May 2011

2011-05-12T04:05:50Z

←Older revision		Revision as of 04:05, 12 May 2011
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-	~~{{Seed}}~~	+	[[Image:2wsx.png\|left\|200px]]
-	[[Image:2wsx.~~jpg~~\|left\|200px]]	+

	<!--		<!--
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	===CRYSTAL STRUCTURE OF CARNITINE TRANSPORTER FROM ESCHERICHIA COLI===		===CRYSTAL STRUCTURE OF CARNITINE TRANSPORTER FROM ESCHERICHIA COLI===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20829798}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20829798 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20829798}}

	==About this Structure==		==About this Structure==
-	~~2WSX~~ is a 3 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA].	+	[[2wsx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSX OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:020829798</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Kuehlbrandt, W.]]		[[Category: Kuehlbrandt, W.]]
	[[Category: Scheltinga, A C.Terwisscha Van.]]		[[Category: Scheltinga, A C.Terwisscha Van.]]
	[[Category: Schulze, S.]]		[[Category: Schulze, S.]]
-	[[Category: Membrane protein]]
-	[[Category: Transport protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Sep 10 13:53:10 2010''