2wuz - Revision history

OCA at 10:17, 20 December 2023

2023-12-20T10:17:05Z

←Older revision		Revision as of 10:17, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2wuz' size='340' side='right'caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2wuz' size='340' side='right'caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Trycr Trycr~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WUZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wx2\|2wx2]], [[2wv2\|2wv2]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [https://pdbe.org/2wuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [https://www.ebi.ac.uk/pdbsum/2wuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wuz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [https://pdbe.org/2wuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [https://www.ebi.ac.uk/pdbsum/2wuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wuz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CP51_TRYCC CP51_TRYCC] Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol (By similarity). Favors C4 dimethylated substrates, the substrate preference order is 24-methylenedihydrolanosterol > 24,25-dihydrolanosterol > lanosterol > obtusifoliol > norlanosterol.<ref>PMID:16321980</ref> [UniProtKB:P0A512]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Sterol 14-demethylase]]~~	+	[[Category: Trypanosoma cruzi]]
-	~~[[Category: Trycr~~]]	+	[[Category: Chen C-K]]
-	[[Category: Chen, C K]]	+	[[Category: Guilbert C]]
-	[[Category: Guilbert, C]]	+	[[Category: Jacobson M]]
-	[[Category: Jacobson, M]]	+	[[Category: Leung SSF]]
-	[[Category: Leung~~, S S.F~~]]	+	[[Category: McKerrow JH]]
-	[[Category: McKerrow~~, J H~~]]	+	[[Category: Podust LM]]
-	[[Category: Podust~~, L M]]~~	+
-	~~[[Category: Cyp51]]~~	+
-	~~[[Category: Ergosterol biosynthesis]]~~	+
-	~~[[Category: Iron]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: P450~~]]	+

OCA at 10:40, 13 April 2022

2022-04-13T10:40:24Z

←Older revision		Revision as of 10:40, 13 April 2022
Line 3:		Line 3:
	<StructureSection load='2wuz' size='340' side='right'caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2wuz' size='340' side='right'caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WUZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wx2\|2wx2]], [[2wv2\|2wv2]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [~~http~~://pdbe.org/2wuz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wuz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wuz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [https://pdbe.org/2wuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [https://www.ebi.ac.uk/pdbsum/2wuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wuz ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 14:57, 8 July 2020

2020-07-08T14:57:43Z

←Older revision		Revision as of 14:57, 8 July 2020
Line 1:		Line 1:

	==X-ray structure of CYP51 from Trypanosoma cruzi in complex with fluconazole in alternative conformation==		==X-ray structure of CYP51 from Trypanosoma cruzi in complex with fluconazole in alternative conformation==
-	<StructureSection load='2wuz' size='340' side='right' caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='2wuz' size='340' side='right'caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WUZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [http://pdbe.org/2wuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [http://www.ebi.ac.uk/pdbsum/2wuz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wuz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [http://pdbe.org/2wuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [http://www.ebi.ac.uk/pdbsum/2wuz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wuz ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Sterol 14-demethylase]]		[[Category: Sterol 14-demethylase]]
	[[Category: Trycr]]		[[Category: Trycr]]

OCA at 09:33, 3 October 2018

2018-10-03T09:33:55Z

←Older revision		Revision as of 09:33, 3 October 2018
Line 1:		Line 1:
		+
	==X-ray structure of CYP51 from Trypanosoma cruzi in complex with fluconazole in alternative conformation==		==X-ray structure of CYP51 from Trypanosoma cruzi in complex with fluconazole in alternative conformation==
	<StructureSection load='2wuz' size='340' side='right' caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2wuz' size='340' side='right' caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [http://pdbe.org/2wuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [http://www.ebi.ac.uk/pdbsum/2wuz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [http://pdbe.org/2wuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [http://www.ebi.ac.uk/pdbsum/2wuz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wuz ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wu/2wuz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wu/2wuz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 37:		Line 38:
	[[Category: Jacobson, M]]		[[Category: Jacobson, M]]
	[[Category: Leung, S S.F]]		[[Category: Leung, S S.F]]
-	[[Category: ~~Mckerrow~~, J H]]	+	[[Category: McKerrow, J H]]
	[[Category: Podust, L M]]		[[Category: Podust, L M]]
	[[Category: Cyp51]]		[[Category: Cyp51]]

OCA at 21:06, 7 February 2016

2016-02-07T21:06:21Z

←Older revision		Revision as of 21:06, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='2wuz' size='340' side='right' caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2wuz' size='340' side='right' caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Trypanosoma_cruzi Trypanosoma cruzi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WUZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [http://www.ebi.ac.uk/pdbsum/2wuz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [http://pdbe.org/2wuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [http://www.ebi.ac.uk/pdbsum/2wuz PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wuz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wuz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Sterol 14-demethylase]]		[[Category: Sterol 14-demethylase]]
-	[[Category: ~~Trypanosoma cruzi~~]]	+	[[Category: Trycr]]
	[[Category: Chen, C K]]		[[Category: Chen, C K]]
	[[Category: Guilbert, C]]		[[Category: Guilbert, C]]

OCA at 09:30, 20 January 2015

2015-01-20T09:30:20Z

←Older revision		Revision as of 09:30, 20 January 2015
Line 32:		Line 32:
	[[Category: Sterol 14-demethylase]]		[[Category: Sterol 14-demethylase]]
	[[Category: Trypanosoma cruzi]]		[[Category: Trypanosoma cruzi]]
-	[[Category: Chen, C K.]]	+	[[Category: Chen, C K]]
-	[[Category: Guilbert, C.]]	+	[[Category: Guilbert, C]]
-	[[Category: Jacobson, M.]]	+	[[Category: Jacobson, M]]
-	[[Category: Leung, S S.F.]]	+	[[Category: Leung, S S.F]]
-	[[Category: Mckerrow, J H.]]	+	[[Category: Mckerrow, J H]]
-	[[Category: Podust, L M.]]	+	[[Category: Podust, L M]]
	[[Category: Cyp51]]		[[Category: Cyp51]]
	[[Category: Ergosterol biosynthesis]]		[[Category: Ergosterol biosynthesis]]

OCA at 06:40, 10 October 2014

2014-10-10T06:40:42Z

←Older revision		Revision as of 06:40, 10 October 2014
Line 2:		Line 2:
	<StructureSection load='2wuz' size='340' side='right' caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2wuz' size='340' side='right' caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. <br>	+	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WUZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.7.~~1.~~2 2~~.7.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [http://www.ebi.ac.uk/pdbsum/2wuz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [http://www.ebi.ac.uk/pdbsum/2wuz PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 24:
	Structural characterization of CYP51 from Trypanosoma cruzi and Trypanosoma brucei bound to the antifungal drugs posaconazole and fluconazole.,Chen CK, Leung SS, Guilbert C, Jacobson MP, McKerrow JH, Podust LM PLoS Negl Trop Dis. 2010 Apr 6;4(4):e651. PMID:20386598<ref>PMID:20386598</ref>		Structural characterization of CYP51 from Trypanosoma cruzi and Trypanosoma brucei bound to the antifungal drugs posaconazole and fluconazole.,Chen CK, Leung SS, Guilbert C, Jacobson MP, McKerrow JH, Podust LM PLoS Negl Trop Dis. 2010 Apr 6;4(4):e651. PMID:20386598<ref>PMID:20386598</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 08:33, 7 May 2014

2014-05-07T08:33:25Z

←Older revision		Revision as of 08:33, 7 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2wuz.~~png~~\|~~left~~\|200px]]	+	==X-ray structure of CYP51 from Trypanosoma cruzi in complex with fluconazole in alternative conformation==
		+	<StructureSection load='2wuz' size='340' side='right' caption='[[2wuz]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wuz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wx2\|2wx2]], [[2wv2\|2wv2]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wuz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wuz RCSB], [http://www.ebi.ac.uk/pdbsum/2wuz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wu/2wuz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Chagas Disease is the leading cause of heart failure in Latin America. Current drug therapy is limited by issues of both efficacy and severe side effects. Trypansoma cruzi, the protozoan agent of Chagas Disease, is closely related to two other major global pathogens, Leishmania spp., responsible for leishmaniasis, and Trypansoma brucei, the causative agent of African Sleeping Sickness. Both T. cruzi and Leishmania parasites have an essential requirement for ergosterol, and are thus vulnerable to inhibitors of sterol 14alpha-demethylase (CYP51), which catalyzes the conversion of lanosterol to ergosterol. Clinically employed anti-fungal azoles inhibit ergosterol biosynthesis in fungi, and specific azoles are also effective against both Trypanosoma and Leishmania parasites. However, modification of azoles to enhance efficacy and circumvent potential drug resistance has been problematic for both parasitic and fungal infections due to the lack of structural insights into drug binding. METHODOLOGY/PRINCIPAL FINDINGS: We have determined the crystal structures for CYP51 from T. cruzi (resolutions of 2.35 A and 2.27 A), and from the related pathogen T. brucei (resolutions of 2.7 A and 2.6 A), co-crystallized with the antifungal drugs fluconazole and posaconazole. Remarkably, both drugs adopt multiple conformations when binding the target. The fluconazole 2,4-difluorophenyl ring flips 180 degrees depending on the H-bonding interactions with the BC-loop. The terminus of the long functional tail group of posaconazole is bound loosely in the mouth of the hydrophobic substrate binding tunnel, suggesting that the major contribution of the tail to drug efficacy is for pharmacokinetics rather than in interactions with the target. CONCLUSIONS/SIGNIFICANCE: The structures provide new insights into binding of azoles to CYP51 and mechanisms of potential drug resistance. Our studies define in structural detail the CYP51 therapeutic target in T. cruzi, and offer a starting point for rationally designed anti-Chagasic drugs with improved efficacy and reduced toxicity.

-	~~<!--~~	+	Structural characterization of CYP51 from Trypanosoma cruzi and Trypanosoma brucei bound to the antifungal drugs posaconazole and fluconazole.,Chen CK, Leung SS, Guilbert C, Jacobson MP, McKerrow JH, Podust LM PLoS Negl Trop Dis. 2010 Apr 6;4(4):e651. PMID:20386598<ref>PMID:20386598</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2wuz"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_2wuz\| PDB=2wuz \| SCENE= }}~~	+

-	~~===X-ray structure~~ of ~~CYP51 from Trypanosoma cruzi in complex with fluconazole in alternative conformation===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_20386598}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 20386598 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_20386598}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2wuz]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUZ OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020386598<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Sterol 14-demethylase]]		[[Category: Sterol 14-demethylase]]
	[[Category: Trypanosoma cruzi]]		[[Category: Trypanosoma cruzi]]

OCA: Protected "2wuz" [edit=sysop:move=sysop]

2012-06-02T09:02:12Z

Protected "2wuz" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:02, 2 June 2012