2wyi - Revision history

OCA at 10:19, 20 December 2023

2023-12-20T10:19:43Z

←Older revision		Revision as of 10:19, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2wyi' size='340' side='right'caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2wyi' size='340' side='right'caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Streptococcus_pyogenes_(serotype_1_/_m1_gas)~~ Streptococcus pyogenes ~~(serotype 1 / m1 gas)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_M1_GAS Streptococcus pyogenes M1 GAS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WYI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wyh\|2wyh]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [https://pdbe.org/2wyi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [https://www.ebi.ac.uk/pdbsum/2wyi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [https://pdbe.org/2wyi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [https://www.ebi.ac.uk/pdbsum/2wyi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q99YP5_STRP1 Q99YP5_STRP1]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Alpha-mannosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Davies~~, G J~~]]	+	[[Category: Streptococcus pyogenes M1 GAS]]
-	[[Category: Gilbert~~, H J~~]]	+	[[Category: Davies GJ]]
-	[[Category: Suits~~, M D.L~~]]	+	[[Category: Gilbert HJ]]
-	[[Category: Taylor~~, E J~~]]	+	[[Category: Suits MDL]]
-	[[Category: Zechel~~, D L~~]]	+	[[Category: Taylor EJ]]
-	[[Category: Zhu, Y]]	+	[[Category: Zechel DL]]
-	~~[[Category: Glycosidase]]~~	+	[[Category: Zhu Y]]
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 10:42, 13 April 2022

2022-04-13T10:42:38Z

←Older revision		Revision as of 10:42, 13 April 2022
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	<StructureSection load='2wyi' size='340' side='right'caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2wyi' size='340' side='right'caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptococcus_pyogenes_(serotype_1_/_m1_gas) Streptococcus pyogenes (serotype 1 / m1 gas)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_(serotype_1_/_m1_gas) Streptococcus pyogenes (serotype 1 / m1 gas)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WYI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wyh\|2wyh]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [~~http~~://pdbe.org/2wyi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wyi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [https://pdbe.org/2wyi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [https://www.ebi.ac.uk/pdbsum/2wyi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 15:00, 8 July 2020

2020-07-08T15:00:38Z

←Older revision		Revision as of 15:00, 8 July 2020
Line 1:		Line 1:

	==Structure of the Streptococcus pyogenes family GH38 alpha-mannosidase complexed with swainsonine==		==Structure of the Streptococcus pyogenes family GH38 alpha-mannosidase complexed with swainsonine==
-	<StructureSection load='2wyi' size='340' side='right' caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='2wyi' size='340' side='right'caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_(serotype_1_/_m1_gas) Streptococcus pyogenes (serotype 1 / m1 gas)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_(serotype_1_/_m1_gas) Streptococcus pyogenes (serotype 1 / m1 gas)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WYI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [http://pdbe.org/2wyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [http://www.ebi.ac.uk/pdbsum/2wyi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [http://pdbe.org/2wyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [http://www.ebi.ac.uk/pdbsum/2wyi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Mannosidase\|Mannosidase]]	+	*[[Mannosidase 3D structures\|Mannosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Alpha-mannosidase]]		[[Category: Alpha-mannosidase]]
		+	[[Category: Large Structures]]
	[[Category: Davies, G J]]		[[Category: Davies, G J]]
	[[Category: Gilbert, H J]]		[[Category: Gilbert, H J]]

OCA at 09:10, 3 October 2018

2018-10-03T09:10:19Z

←Older revision		Revision as of 09:10, 3 October 2018
Line 1:		Line 1:
-	==~~STRUCTURE OF THE STREPTOCOCCUS PYOGENES FAMILY~~ GH38 ~~ALPHA~~-~~MANNOSIDASE COMPLEXED WITH SWAINSONINE~~==	+
		+	==Structure of the Streptococcus pyogenes family GH38 alpha-mannosidase complexed with swainsonine==
	<StructureSection load='2wyi' size='340' side='right' caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2wyi' size='340' side='right' caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [http://pdbe.org/2wyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [http://www.ebi.ac.uk/pdbsum/2wyi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [http://pdbe.org/2wyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [http://www.ebi.ac.uk/pdbsum/2wyi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wy/2wyi_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wy/2wyi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 00:35, 9 February 2016

2016-02-09T00:35:01Z

←Older revision		Revision as of 00:35, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2wyi' size='340' side='right' caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2wyi' size='340' side='right' caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_pyogenes~~ Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_(serotype_1_/_m1_gas) Streptococcus pyogenes (serotype 1 / m1 gas)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WYI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [http://www.ebi.ac.uk/pdbsum/2wyi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [http://pdbe.org/2wyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [http://www.ebi.ac.uk/pdbsum/2wyi PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wyi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wyi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Alpha-mannosidase]]		[[Category: Alpha-mannosidase]]
-	[[Category: Streptococcus pyogenes]]
	[[Category: Davies, G J]]		[[Category: Davies, G J]]
	[[Category: Gilbert, H J]]		[[Category: Gilbert, H J]]

OCA at 08:57, 20 January 2015

2015-01-20T08:57:44Z

←Older revision		Revision as of 08:57, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WYI FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WYI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [http://www.ebi.ac.uk/pdbsum/2wyi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [http://www.ebi.ac.uk/pdbsum/2wyi PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Alpha-mannosidase]]		[[Category: Alpha-mannosidase]]
	[[Category: Streptococcus pyogenes]]		[[Category: Streptococcus pyogenes]]
-	[[Category: Davies, G J.]]	+	[[Category: Davies, G J]]
-	[[Category: Gilbert, H J.]]	+	[[Category: Gilbert, H J]]
-	[[Category: Suits, M D.L.]]	+	[[Category: Suits, M D.L]]
-	[[Category: Taylor, E J.]]	+	[[Category: Taylor, E J]]
-	[[Category: Zechel, D L.]]	+	[[Category: Zechel, D L]]
-	[[Category: Zhu, Y.]]	+	[[Category: Zhu, Y]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
	[[Category: Glycoside hydrolase]]		[[Category: Glycoside hydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:21, 29 September 2014

2014-09-29T07:21:06Z

←Older revision		Revision as of 07:21, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2wyi.~~png~~\|~~left~~\|200px]]	+	==STRUCTURE OF THE STREPTOCOCCUS PYOGENES FAMILY GH38 ALPHA-MANNOSIDASE COMPLEXED WITH SWAINSONINE==
		+	<StructureSection load='2wyi' size='340' side='right' caption='[[2wyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wyi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WYI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wyh\|2wyh]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wyi RCSB], [http://www.ebi.ac.uk/pdbsum/2wyi PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wy/2wyi_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: The enzymatic hydrolysis of alpha-mannosides is catalyzed by glycoside hydrolases (GH), termed alpha-mannosidases. These enzymes are found in different GH sequence-based families. Considerable research has probed the role of higher eukaryotic "GH38" alpha-mannosides that play a key role in the modification and diversification of hybrid N-glycans; processes with strong cellular links to cancer and autoimmune disease. The most extensively studied of these enzymes is the Drosophila GH38 alpha-mannosidase II, which has been shown to be a retaining alpha-mannosidase that targets both alpha-1,3 and alpha-1,6 mannosyl linkages, an activity that enables the enzyme to process GlcNAc(Man)(5)(GlcNAc)(2) hybrid N-glycans to GlcNAc(Man)(3)(GlcNAc)(2). Far less well understood is the observation that many bacterial species, predominantly but not exclusively pathogens and symbionts, also possess putative GH38 alpha-mannosidases whose activity and specificity is unknown. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that the Streptococcus pyogenes (M1 GAS SF370) GH38 enzyme (Spy1604; hereafter SpGH38) is an alpha-mannosidase with specificity for alpha-1,3 mannosidic linkages. The 3D X-ray structure of SpGH38, obtained in native form at 1.9 A resolution and in complex with the inhibitor swainsonine (K(i) 18 microM) at 2.6 A, reveals a canonical GH38 five-domain structure in which the catalytic "-1" subsite shows high similarity with the Drosophila enzyme, including the catalytic Zn(2+) ion. In contrast, the "leaving group" subsites of SpGH38 display considerable differences to the higher eukaryotic GH38s; features that contribute to their apparent specificity. CONCLUSIONS/SIGNIFICANCE: Although the in vivo function of this streptococcal GH38 alpha-mannosidase remains unknown, it is shown to be an alpha-mannosidase active on N-glycans. SpGH38 lies on an operon that also contains the GH84 hexosaminidase (Spy1600) and an additional putative glycosidase. The activity of SpGH38, together with its genomic context, strongly hints at a function in the degradation of host N- or possibly O-glycans. The absence of any classical signal peptide further suggests that SpGH38 may be intracellular, perhaps functioning in the subsequent degradation of extracellular host glycans following their initial digestion by secreted glycosidases.

-	~~{{STRUCTURE_2wyi\| PDB=2wyi \| SCENE= }}~~	+	Structure and Kinetic Investigation of Streptococcus pyogenes Family GH38 alpha-Mannosidase.,Suits MD, Zhu Y, Taylor EJ, Walton J, Zechel DL, Gilbert HJ, Davies GJ PLoS One. 2010 Feb 3;5(2):e9006. PMID:20140249<ref>PMID:20140249</ref>

-	~~===STRUCTURE OF THE STREPTOCOCCUS PYOGENES FAMILY GH38 ALPHA-MANNOSIDASE COMPLEXED WITH SWAINSONINE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20140249}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2wyi]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA].~~	+

	==See Also==		==See Also==
	*[[Mannosidase\|Mannosidase]]		*[[Mannosidase\|Mannosidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020140249<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Alpha-mannosidase]]		[[Category: Alpha-mannosidase]]
	[[Category: Streptococcus pyogenes]]		[[Category: Streptococcus pyogenes]]

OCA at 12:01, 20 October 2012

2012-10-20T12:01:12Z

←Older revision		Revision as of 12:01, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2wyi]] is a 2 chain structure ~~of [[Mannosidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA].	+	[[2wyi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA].

	==See Also==		==See Also==

OCA: Protected "2wyi" [edit=sysop:move=sysop]

2012-07-26T07:21:26Z

Protected "2wyi" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:21, 26 July 2012

OCA at 07:21, 26 July 2012

2012-07-26T07:21:24Z

←Older revision		Revision as of 07:21, 26 July 2012
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:2wyi.png\|left\|200px]]
-	[[Image:2wyi.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2wyi", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2wyi\| PDB=2wyi \| SCENE= }}		{{STRUCTURE_2wyi\| PDB=2wyi \| SCENE= }}

	===STRUCTURE OF THE STREPTOCOCCUS PYOGENES FAMILY GH38 ALPHA-MANNOSIDASE COMPLEXED WITH SWAINSONINE===		===STRUCTURE OF THE STREPTOCOCCUS PYOGENES FAMILY GH38 ALPHA-MANNOSIDASE COMPLEXED WITH SWAINSONINE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20140249}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20140249 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20140249}}		{{ABSTRACT_PUBMED_20140249}}

	==About this Structure==		==About this Structure==
-	~~2WYI~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA].	+	[[2wyi]] is a 2 chain structure of [[Mannosidase]] with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYI OCA].
		+
		+	==See Also==
		+	*[[Mannosidase\|Mannosidase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20140249~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020140249</ref><references group="xtra"/>
	[[Category: Alpha-mannosidase]]		[[Category: Alpha-mannosidase]]
	[[Category: Streptococcus pyogenes]]		[[Category: Streptococcus pyogenes]]
Line 35:		Line 26:
	[[Category: Glycoside hydrolase]]		[[Category: Glycoside hydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 10:30:57 2010''