2x2j - Revision history

OCA at 10:22, 20 December 2023

2023-12-20T10:22:37Z

←Older revision		Revision as of 10:22, 20 December 2023
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	<StructureSection load='2x2j' size='340' side='right'caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2x2j' size='340' side='right'caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x2j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cordylecladia_lemanaeformis Cordylecladia lemanaeformis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x2j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gracilariopsis_lemaneiformis Gracilariopsis lemaneiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X2J FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~CSO~~:~~S-HYDROXYCYSTEINE~~'>~~CSO~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;~~'>~~[[2x2i\|2x2i]], [[2x2h\|2x2h]]~~</~~div~~><~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Exo-(~~1->~~4)-alpha-D-glucan_lyase Exo-(1->4)-alpha-D-glucan lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.13 4.2.2.13]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2j OCA], [https://pdbe.org/2x2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x2j RCSB], [https://www.ebi.ac.uk/pdbsum/2x2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2j OCA], [https://pdbe.org/2x2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x2j RCSB], [https://www.ebi.ac.uk/pdbsum/2x2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9STC1_GRALE Q9STC1_GRALE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cordylecladia lemanaeformis~~]]	+	[[Category: Gracilariopsis lemaneiformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dijkstra~~, B W~~]]	+	[[Category: Dijkstra BW]]
-	[[Category: Kalk~~, K H~~]]	+	[[Category: Kalk KH]]
-	[[Category: Madrid, S]]	+	[[Category: Madrid S]]
-	[[Category: Rozeboom~~, H J~~]]	+	[[Category: Rozeboom HJ]]
-	[[Category: Yu, S]]	+	[[Category: Yu S]]
-	~~[[Category: Anhydrofructose pathway]]~~	+
-	~~[[Category: Glycoside hydrolase family 31]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Starch binding domain~~]]	+

OCA at 05:24, 10 August 2022

2022-08-10T05:24:17Z

←Older revision		Revision as of 05:24, 10 August 2022
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	<StructureSection load='2x2j' size='340' side='right'caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2x2j' size='340' side='right'caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x2j]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cordylecladia_lemanaeformis Cordylecladia lemanaeformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2X2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x2j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cordylecladia_lemanaeformis Cordylecladia lemanaeformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X2J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x2i\|2x2i]], [[2x2h\|2x2h]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2x2i\|2x2i]], [[2x2h\|2x2h]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Exo-(1->4)-alpha-D-glucan_lyase Exo-(1->4)-alpha-D-glucan lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.13 4.2.2.13] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Exo-(1->4)-alpha-D-glucan_lyase Exo-(1->4)-alpha-D-glucan lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.13 4.2.2.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2x2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2j OCA], [~~http~~://pdbe.org/2x2j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2x2j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2x2j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2j OCA], [https://pdbe.org/2x2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x2j RCSB], [https://www.ebi.ac.uk/pdbsum/2x2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:00, 4 November 2020

2020-11-04T08:00:51Z

←Older revision		Revision as of 08:00, 4 November 2020
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	==Crystal structure of the Gracilariopsis lemaneiformis alpha- 1,4-glucan lyase with deoxynojirimycin==		==Crystal structure of the Gracilariopsis lemaneiformis alpha- 1,4-glucan lyase with deoxynojirimycin==
-	<StructureSection load='2x2j' size='340' side='right' caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='2x2j' size='340' side='right'caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x2j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cordylecladia_lemanaeformis Cordylecladia lemanaeformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2X2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x2j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cordylecladia_lemanaeformis Cordylecladia lemanaeformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2X2J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x2i\|2x2i]], [[2x2h\|2x2h]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x2i\|2x2i]], [[2x2h\|2x2h]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-(1->4)-alpha-D-glucan_lyase Exo-(1->4)-alpha-D-glucan lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.13 4.2.2.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-(1->4)-alpha-D-glucan_lyase Exo-(1->4)-alpha-D-glucan lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.13 4.2.2.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2x2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2j OCA], [http://pdbe.org/2x2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x2j RCSB], [http://www.ebi.ac.uk/pdbsum/2x2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2x2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2j OCA], [http://pdbe.org/2x2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x2j RCSB], [http://www.ebi.ac.uk/pdbsum/2x2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Cordylecladia lemanaeformis]]		[[Category: Cordylecladia lemanaeformis]]
		+	[[Category: Large Structures]]
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]
	[[Category: Kalk, K H]]		[[Category: Kalk, K H]]

OCA at 07:32, 16 November 2017

2017-11-16T07:32:03Z

←Older revision		Revision as of 07:32, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='2x2j' size='340' side='right' caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2x2j' size='340' side='right' caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x2j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gracilariopsis_lemaneiformis Gracilariopsis lemaneiformis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x2j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cordylecladia_lemanaeformis Cordylecladia lemanaeformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X2J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Gracilariopsis lemaneiformis~~]]	+	[[Category: Cordylecladia lemanaeformis]]
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]
	[[Category: Kalk, K H]]		[[Category: Kalk, K H]]

OCA at 08:05, 5 August 2016

2016-08-05T08:05:02Z

←Older revision		Revision as of 08:05, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the Gracilariopsis lemaneiformis alpha- 1,4-glucan lyase with deoxynojirimycin==		==Crystal structure of the Gracilariopsis lemaneiformis alpha- 1,4-glucan lyase with deoxynojirimycin==
	<StructureSection load='2x2j' size='340' side='right' caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2x2j' size='340' side='right' caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x2i\|2x2i]], [[2x2h\|2x2h]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x2i\|2x2i]], [[2x2h\|2x2h]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-(1->4)-alpha-D-glucan_lyase Exo-(1->4)-alpha-D-glucan lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.13 4.2.2.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-(1->4)-alpha-D-glucan_lyase Exo-(1->4)-alpha-D-glucan lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.13 4.2.2.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x2j RCSB], [http://www.ebi.ac.uk/pdbsum/2x2j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2j OCA], [http://pdbe.org/2x2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x2j RCSB], [http://www.ebi.ac.uk/pdbsum/2x2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2x2j" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:34, 18 December 2014

2014-12-18T11:34:49Z

←Older revision		Revision as of 11:34, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2x2j\| PDB~~=2x2j \| ~~SCENE~~= }}	+	==Crystal structure of the Gracilariopsis lemaneiformis alpha- 1,4-glucan lyase with deoxynojirimycin==
-	===~~Crystal~~ structure ~~of the~~ Gracilariopsis lemaneiformis ~~alpha~~- 1,4-glucan lyase with ~~deoxynojirimycin~~===	+	<StructureSection load='2x2j' size='340' side='right' caption='[[2x2j]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23902768}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2x2j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gracilariopsis_lemaneiformis Gracilariopsis lemaneiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X2J FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x2i\|2x2i]], [[2x2h\|2x2h]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-(1->4)-alpha-D-glucan_lyase Exo-(1->4)-alpha-D-glucan lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.13 4.2.2.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x2j RCSB], [http://www.ebi.ac.uk/pdbsum/2x2j PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	alpha-1,4-Glucan lyase (EC 4.2.2.13) from the red seaweed Gracilariopsis lemaneiformis cleaves alpha-1,4-glucosidic linkages in glycogen, starch and malto-oligosaccharides, yielding the keto-monosaccharide 1,5-anhydro-D-fructose. The enzyme belongs to glycoside hydrolase family 31 (GH31), but degrades starch via an elimination reaction instead of hydrolysis. The crystal structure shows that the enzyme, like GH31 hydrolases, contains a (beta/alpha)8-barrel catalytic domain with B and B' subdomains, an N-terminal domain N, and the C-terminal domains C and D. The N-terminal domain N of the lyase was found to bind a trisaccharide. Complexes of the enzyme with acarbose and 1-dexoynojirimycin, and two different covalent glycosyl-enzyme intermediates obtained with fluorinated sugar analogues show that, like GH31 hydrolases, the aspartic acid residues D553 and D665 are the catalytic nucleophile and acid, respectively. However, as a unique feature, the catalytic nucleophile is in a position to act also as a base that abstracts a proton from the C2 carbon atom of the covalently bound subsite -1 glucosyl residue, thus explaining the enzyme's unique lyase activity. One Glu to Val mutation in the active site of the homologous alpha-glucosidase from Sulfolobus solfataricus resulted in a shift from hydrolytic to lyase activity, demonstrating that a subtle amino acid difference can promote lyase activity in a GH31 hydrolase.

-	~~==About this Structure==~~	+	Crystal structure of alpha-1,4-glucan lyase, a unique glycoside hydrolase family member with a novel catalytic mechanism.,Rozeboom HJ, Yu S, Madrid S, Kalk KH, Zhang R, Dijkstra BW J Biol Chem. 2013 Jul 31. PMID:23902768<ref>PMID:23902768</ref>
-	~~[[2x2j]] is~~ a ~~4 chain structure~~ with ~~sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Gracilariopsis_lemaneiformis Gracilariopsis lemaneiformis]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023902768</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Gracilariopsis lemaneiformis]]		[[Category: Gracilariopsis lemaneiformis]]
-	[[Category: Dijkstra, B W.]]	+	[[Category: Dijkstra, B W]]
-	[[Category: Kalk, K H.]]	+	[[Category: Kalk, K H]]
-	[[Category: Madrid, S.]]	+	[[Category: Madrid, S]]
-	[[Category: Rozeboom, H J.]]	+	[[Category: Rozeboom, H J]]
-	[[Category: Yu, S.]]	+	[[Category: Yu, S]]
	[[Category: Anhydrofructose pathway]]		[[Category: Anhydrofructose pathway]]
	[[Category: Glycoside hydrolase family 31]]		[[Category: Glycoside hydrolase family 31]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Starch binding domain]]		[[Category: Starch binding domain]]

OCA at 06:33, 14 August 2013

2013-08-14T06:33:54Z

←Older revision		Revision as of 06:33, 14 August 2013
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-	[[Image:2x2j.png\|left\|200px]]
-
	{{STRUCTURE_2x2j\| PDB=2x2j \| SCENE= }}		{{STRUCTURE_2x2j\| PDB=2x2j \| SCENE= }}
-		+	===Crystal structure of the Gracilariopsis lemaneiformis alpha- 1,4-glucan lyase with deoxynojirimycin===
-	===~~CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA~~-1,4-~~GLUCAN LYASE WITH DEOXYNOJIRIMYCIN~~===	+	{{ABSTRACT_PUBMED_23902768}}
-		+

	==About this Structure==		==About this Structure==
	[[2x2j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gracilariopsis_lemaneiformis Gracilariopsis lemaneiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA].		[[2x2j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gracilariopsis_lemaneiformis Gracilariopsis lemaneiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023902768</ref><references group="xtra"/><references/>
	[[Category: Gracilariopsis lemaneiformis]]		[[Category: Gracilariopsis lemaneiformis]]
	[[Category: Dijkstra, B W.]]		[[Category: Dijkstra, B W.]]

OCA at 20:39, 7 January 2013

2013-01-07T20:39:30Z

←Older revision		Revision as of 20:39, 7 January 2013
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-	[[Image:2x2j.~~jpg~~\|left\|200px]]	+	[[Image:2x2j.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2x2j", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2x2j\| PDB=2x2j \| SCENE= }}		{{STRUCTURE_2x2j\| PDB=2x2j \| SCENE= }}

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	[[Category: Rozeboom, H J.]]		[[Category: Rozeboom, H J.]]
	[[Category: Yu, S.]]		[[Category: Yu, S.]]
		+	[[Category: Anhydrofructose pathway]]
		+	[[Category: Glycoside hydrolase family 31]]
		+	[[Category: Lyase]]
		+	[[Category: Starch binding domain]]

OCA: Protected "2x2j" [edit=sysop:move=sysop]

2011-01-19T06:09:44Z

Protected "2x2j" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:09, 19 January 2011

OCA at 06:09, 19 January 2011

2011-01-19T06:09:41Z

←Older revision		Revision as of 06:09, 19 January 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:2x2j.jpg\|left\|200px]]

-	The ~~entry 2x2j~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2x2j", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2x2j\| PDB=2x2j \| SCENE= }}

-	~~Authors: Rozeboom~~, ~~H.J., Yu, S., Madrid, S., Kalk, K.H., Dijkstra, B.W.~~	+	===CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA-1,4-GLUCAN LYASE WITH DEOXYNOJIRIMYCIN===

-	Description: Crystal structure of the Gracilariopsis lemaneiformis alpha-1,4-glucan lyase with deoxynojirimycin

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Jan 27 19~~:10:~~19 2010''~~	+	==About this Structure==
		+	[[2x2j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gracilariopsis_lemaneiformis Gracilariopsis lemaneiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2J OCA].
		+	[[Category: Gracilariopsis lemaneiformis]]
		+	[[Category: Dijkstra, B W.]]
		+	[[Category: Kalk, K H.]]
		+	[[Category: Madrid, S.]]
		+	[[Category: Rozeboom, H J.]]
		+	[[Category: Yu, S.]]