2xge - Revision history

OCA at 10:31, 20 December 2023

2023-12-20T10:31:08Z

←Older revision		Revision as of 10:31, 20 December 2023
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	<StructureSection load='2xge' size='340' side='right'caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='2xge' size='340' side='right'caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xge]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xge]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XGE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.14Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xgc\|2xgc]], [[2x9d\|2x9d]], [[1bjz\|1bjz]], [[2trt\|2trt]], [[2vkv\|2vkv]], [[1ork\|1ork]], [[2x6o\|2x6o]], [[1a6i\|1a6i]], [[2vke\|2vke]], [[1qpi\|1qpi]], [[2xgd\|2xgd]], [[1du7\|1du7]], [[2xb5\|2xb5]], [[1bj0\|1bj0]], [[2tct\|2tct]], [[1bjy\|1bjy]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [https://pdbe.org/2xge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [https://www.ebi.ac.uk/pdbsum/2xge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xge ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [https://pdbe.org/2xge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [https://www.ebi.ac.uk/pdbsum/2xge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xge ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.	+	[https://www.uniprot.org/uniprot/TETR4_ECOLX TETR4_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Muller~~, Y A~~]]	+	[[Category: Muller YA]]
-	[[Category: Richter~~, J P~~]]	+	[[Category: Richter JP]]
-	[[Category: Stiebritz~~, M T~~]]	+	[[Category: Stiebritz MT]]
-	[[Category: Wengrzik, S]]	+	[[Category: Wengrzik S]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Chimera]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation~~]]	+

OCA at 10:52, 13 April 2022

2022-04-13T10:52:15Z

←Older revision		Revision as of 10:52, 13 April 2022
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	<StructureSection load='2xge' size='340' side='right'caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='2xge' size='340' side='right'caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xge]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xge]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XGE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xgc\|2xgc]], [[2x9d\|2x9d]], [[1bjz\|1bjz]], [[2trt\|2trt]], [[2vkv\|2vkv]], [[1ork\|1ork]], [[2x6o\|2x6o]], [[1a6i\|1a6i]], [[2vke\|2vke]], [[1qpi\|1qpi]], [[2xgd\|2xgd]], [[1du7\|1du7]], [[2xb5\|2xb5]], [[1bj0\|1bj0]], [[2tct\|2tct]], [[1bjy\|1bjy]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xgc\|2xgc]], [[2x9d\|2x9d]], [[1bjz\|1bjz]], [[2trt\|2trt]], [[2vkv\|2vkv]], [[1ork\|1ork]], [[2x6o\|2x6o]], [[1a6i\|1a6i]], [[2vke\|2vke]], [[1qpi\|1qpi]], [[2xgd\|2xgd]], [[1du7\|1du7]], [[2xb5\|2xb5]], [[1bj0\|1bj0]], [[2tct\|2tct]], [[1bjy\|1bjy]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [~~http~~://pdbe.org/2xge PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xge PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xge ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [https://pdbe.org/2xge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [https://www.ebi.ac.uk/pdbsum/2xge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xge ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.	+	[[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 15:13, 8 July 2020

2020-07-08T15:13:41Z

←Older revision		Revision as of 15:13, 8 July 2020
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	==Crystal structure of a designed heterodimeric variant T-A(A)B of the tetracycline repressor==		==Crystal structure of a designed heterodimeric variant T-A(A)B of the tetracycline repressor==
-	<StructureSection load='2xge' size='340' side='right' caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>	+	<StructureSection load='2xge' size='340' side='right'caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XGE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xgc\|2xgc]], [[2x9d\|2x9d]], [[1bjz\|1bjz]], [[2trt\|2trt]], [[2vkv\|2vkv]], [[1ork\|1ork]], [[2x6o\|2x6o]], [[1a6i\|1a6i]], [[2vke\|2vke]], [[1qpi\|1qpi]], [[2xgd\|2xgd]], [[1du7\|1du7]], [[2xb5\|2xb5]], [[1bj0\|1bj0]], [[2tct\|2tct]], [[1bjy\|1bjy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xgc\|2xgc]], [[2x9d\|2x9d]], [[1bjz\|1bjz]], [[2trt\|2trt]], [[2vkv\|2vkv]], [[1ork\|1ork]], [[2x6o\|2x6o]], [[1a6i\|1a6i]], [[2vke\|2vke]], [[1qpi\|1qpi]], [[2xgd\|2xgd]], [[1du7\|1du7]], [[2xb5\|2xb5]], [[1bj0\|1bj0]], [[2tct\|2tct]], [[1bjy\|1bjy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [http://pdbe.org/2xge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [http://www.ebi.ac.uk/pdbsum/2xge PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xge ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [http://pdbe.org/2xge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [http://www.ebi.ac.uk/pdbsum/2xge PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xge ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Tetracycline repressor protein\|Tetracycline repressor protein]]	+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Muller, Y A]]		[[Category: Muller, Y A]]
	[[Category: Richter, J P]]		[[Category: Richter, J P]]

OCA at 09:15, 3 October 2018

2018-10-03T09:15:41Z

←Older revision		Revision as of 09:15, 3 October 2018
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-	==~~CRYSTAL STRUCTURE OF A DESIGNED HETERODIMERIC VARIANT~~ T-A(A) B ~~OF THE TETRACYCLINE REPRESSOR~~==	+	==Crystal structure of a designed heterodimeric variant T-A(A)B of the tetracycline repressor==
	<StructureSection load='2xge' size='340' side='right' caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='2xge' size='340' side='right' caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xg/2xge_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xg/2xge_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:09, 4 August 2016

2016-08-04T17:09:34Z

←Older revision		Revision as of 17:09, 4 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF A DESIGNED HETERODIMERIC VARIANT T-A(A) B OF THE TETRACYCLINE REPRESSOR==		==CRYSTAL STRUCTURE OF A DESIGNED HETERODIMERIC VARIANT T-A(A) B OF THE TETRACYCLINE REPRESSOR==
	<StructureSection load='2xge' size='340' side='right' caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='2xge' size='340' side='right' caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XGE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xgc\|2xgc]], [[2x9d\|2x9d]], [[1bjz\|1bjz]], [[2trt\|2trt]], [[2vkv\|2vkv]], [[1ork\|1ork]], [[2x6o\|2x6o]], [[1a6i\|1a6i]], [[2vke\|2vke]], [[1qpi\|1qpi]], [[2xgd\|2xgd]], [[1du7\|1du7]], [[2xb5\|2xb5]], [[1bj0\|1bj0]], [[2tct\|2tct]], [[1bjy\|1bjy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xgc\|2xgc]], [[2x9d\|2x9d]], [[1bjz\|1bjz]], [[2trt\|2trt]], [[2vkv\|2vkv]], [[1ork\|1ork]], [[2x6o\|2x6o]], [[1a6i\|1a6i]], [[2vke\|2vke]], [[1qpi\|1qpi]], [[2xgd\|2xgd]], [[1du7\|1du7]], [[2xb5\|2xb5]], [[1bj0\|1bj0]], [[2tct\|2tct]], [[1bjy\|1bjy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [http://www.ebi.ac.uk/pdbsum/2xge PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [http://pdbe.org/2xge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [http://www.ebi.ac.uk/pdbsum/2xge PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xge ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xge ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xge" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Muller, Y A]]		[[Category: Muller, Y A]]
	[[Category: Richter, J P]]		[[Category: Richter, J P]]

OCA at 17:23, 24 December 2014

2014-12-24T17:23:26Z

←Older revision		Revision as of 17:23, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [http://www.ebi.ac.uk/pdbsum/2xge PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [http://www.ebi.ac.uk/pdbsum/2xge PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Muller, Y A.]]	+	[[Category: Muller, Y A]]
-	[[Category: Richter, J P.]]	+	[[Category: Richter, J P]]
-	[[Category: Stiebritz, M T.]]	+	[[Category: Stiebritz, M T]]
-	[[Category: Wengrzik, S.]]	+	[[Category: Wengrzik, S]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Chimera]]		[[Category: Chimera]]
	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

OCA at 13:10, 22 October 2014

2014-10-22T13:10:44Z

←Older revision		Revision as of 13:10, 22 October 2014
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-	[[~~Image:~~2xge.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF A DESIGNED HETERODIMERIC VARIANT T-A(A) B OF THE TETRACYCLINE REPRESSOR==
		+	<StructureSection load='2xge' size='340' side='right' caption='[[2xge]], [[Resolution\|resolution]] 2.14Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XGE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xgc\|2xgc]], [[2x9d\|2x9d]], [[1bjz\|1bjz]], [[2trt\|2trt]], [[2vkv\|2vkv]], [[1ork\|1ork]], [[2x6o\|2x6o]], [[1a6i\|1a6i]], [[2vke\|2vke]], [[1qpi\|1qpi]], [[2xgd\|2xgd]], [[1du7\|1du7]], [[2xb5\|2xb5]], [[1bj0\|1bj0]], [[2tct\|2tct]], [[1bjy\|1bjy]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xge RCSB], [http://www.ebi.ac.uk/pdbsum/2xge PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xg/2xge_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The specificity and selectivity of protein-protein interactions are of central importance for many biological processes, including signal transduction and transcription control. We used the in-house side-chain packing program MUMBO to computationally design a chain-specific heterodimeric variant of the bacterial transcription regulator tetracycline repressor (TetR), called T-A(A)B. Our goal was to engineer two different TetR chain variants, A and B, that no longer interact as AA or BB homodimers but selectively recombine to form heterodimers. Although 56 residues from each chain contribute to a dimer interface as large as 2200 A(2) in wild-type TetR, the substitution of only three residues in one chain and two residues in a second chain sufficed for generating specificity in a T-A(A)B heterodimer variant. The design was corroborated in vivo by a cell-based transcription assay, and in vitro by CD spectroscopy and X-ray crystallography. Crystal structure analyses showed that while selectivity in the B chain is achieved entirely through van der Waals repulsion, the best selectivity in the A chain is obtained for the variant with the lowest number of atoms in the interface, thus possibly leading to underpacking of the dimer interface. This results in a marked decrease in thermal stability and a drastic reduction in the solubility of the T-A(A)A(A) homodimer in comparison to the designed T-A(A)B heterodimer variant.

-	~~{{STRUCTURE_2xge\| PDB=2xge \| SCENE= }}~~	+	Computational Design of a Chain-Specific Tetracycline Repressor Heterodimer.,Stiebritz MT, Wengrzik S, Klein DL, Richter JP, Srebrzynski A, Weiler S, Muller YA J Mol Biol. 2010 Sep 10. PMID:20816982<ref>PMID:20816982</ref>

-	~~===CRYSTAL STRUCTURE OF A DESIGNED HETERODIMERIC VARIANT T-A(A) B OF THE TETRACYCLINE REPRESSOR===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_20816982}}~~	+	==See Also==
-		+	*[[Tetracycline repressor protein\|Tetracycline repressor protein]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~2xge~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:020816982<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Muller, Y A.]]		[[Category: Muller, Y A.]]

OCA: Protected "2xge" [edit=sysop:move=sysop]

2013-01-07T17:53:41Z

Protected "2xge" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:53, 7 January 2013

OCA at 17:53, 7 January 2013

2013-01-07T17:53:37Z

←Older revision		Revision as of 17:53, 7 January 2013
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-	{{Seed}}
	[[Image:2xge.png\|left\|200px]]		[[Image:2xge.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xge", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2xge\| PDB=2xge \| SCENE= }}		{{STRUCTURE_2xge\| PDB=2xge \| SCENE= }}

	===CRYSTAL STRUCTURE OF A DESIGNED HETERODIMERIC VARIANT T-A(A) B OF THE TETRACYCLINE REPRESSOR===		===CRYSTAL STRUCTURE OF A DESIGNED HETERODIMERIC VARIANT T-A(A) B OF THE TETRACYCLINE REPRESSOR===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20816982}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20816982 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_20816982}}		{{ABSTRACT_PUBMED_20816982}}

	==About this Structure==		==About this Structure==
-	~~2XGE~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA].	+	[[2xge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGE OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20816982~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020816982</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Muller, Y A.]]		[[Category: Muller, Y A.]]
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	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 20 06:05:18 2010''

OCA at 02:55, 20 October 2010

2010-10-20T02:55:13Z

←Older revision		Revision as of 02:55, 20 October 2010
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	{{Seed}}		{{Seed}}
-	[[Image:2xge.~~jpg~~\|left\|200px]]	+	[[Image:2xge.png\|left\|200px]]

	<!--		<!--
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	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 22 14~~:42:56 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 20 06:05:18 2010''