2xjc - Revision history

OCA at 10:32, 20 December 2023

2023-12-20T10:32:47Z

←Older revision		Revision as of 10:32, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2xjc' size='340' side='right'caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2xjc' size='340' side='right'caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xje\|2xje]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2xjb\|2xjb]], [[2jcm\|2jcm]], [[2xjf\|2xjf]], [[2xcx\|2xcx]], [[2xcw\|2xcw]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjc OCA], [https://pdbe.org/2xjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xjc RCSB], [https://www.ebi.ac.uk/pdbsum/2xjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjc OCA], [https://pdbe.org/2xjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xjc RCSB], [https://www.ebi.ac.uk/pdbsum/2xjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjc ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>	+	[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.	+	[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~5'-nucleotidase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Nordlund, P]]	+	[[Category: Nordlund P]]
-	[[Category: Wallden, K]]	+	[[Category: Wallden K]]
-	~~[[Category: Allosteric enzyme]]~~	+
-	~~[[Category: Cn-ii]]~~	+
-	~~[[Category: Gmp-imp specific nucleotidase]]~~	+
-	~~[[Category: High km 5-prime nucleotidase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Nt5c2]]~~	+
-	~~[[Category: Nucleotide metabolism]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Phosphoprotein~~]]	+

OCA at 10:54, 13 April 2022

2022-04-13T10:54:05Z

OCA at 15:16, 8 July 2020

2020-07-08T15:16:02Z

←Older revision		Revision as of 15:16, 8 July 2020
Line 1:		Line 1:

	==Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II in complex with guanosine monophosphate and diadenosine tetraphosphate==		==Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II in complex with guanosine monophosphate and diadenosine tetraphosphate==
-	<StructureSection load='2xjc' size='340' side='right' caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2xjc' size='340' side='right'caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XJC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xje\|2xje]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2xjb\|2xjb]], [[2jcm\|2jcm]], [[2xjf\|2xjf]], [[2xcx\|2xcx]], [[2xcw\|2xcw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xje\|2xje]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2xjb\|2xjb]], [[2jcm\|2jcm]], [[2xjf\|2xjf]], [[2xcx\|2xcx]], [[2xcw\|2xcw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjc OCA], [http://pdbe.org/2xjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjc RCSB], [http://www.ebi.ac.uk/pdbsum/2xjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjc OCA], [http://pdbe.org/2xjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjc RCSB], [http://www.ebi.ac.uk/pdbsum/2xjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjc ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 28:		Line 28:
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Nordlund, P]]		[[Category: Nordlund, P]]
	[[Category: Wallden, K]]		[[Category: Wallden, K]]

OCA at 09:48, 3 October 2018

2018-10-03T09:48:31Z

←Older revision		Revision as of 09:48, 3 October 2018
Line 1:		Line 1:

-	==~~CRYSTAL STRUCTURE OF THE~~ D52N ~~VARIANT OF CYTOSOLIC~~ 5'-~~NUCLEOTIDASE~~ II ~~IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE~~==	+	==Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II in complex with guanosine monophosphate and diadenosine tetraphosphate==
	<StructureSection load='2xjc' size='340' side='right' caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2xjc' size='340' side='right' caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 20:32, 9 December 2016

2016-12-09T20:32:17Z

←Older revision		Revision as of 20:32, 9 December 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE==		==CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE==
	<StructureSection load='2xjc' size='340' side='right' caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2xjc' size='340' side='right' caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xje\|2xje]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2xjb\|2xjb]], [[2jcm\|2jcm]], [[2xjf\|2xjf]], [[2xcx\|2xcx]], [[2xcw\|2xcw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xje\|2xje]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2xjb\|2xjb]], [[2jcm\|2jcm]], [[2xjf\|2xjf]], [[2xcx\|2xcx]], [[2xcw\|2xcw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xjc RCSB], [http://www.ebi.ac.uk/pdbsum/2xjc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjc OCA], [http://pdbe.org/2xjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjc RCSB], [http://www.ebi.ac.uk/pdbsum/2xjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjc ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 20:		Line 21:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xjc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Nordlund, P]]		[[Category: Nordlund, P]]
	[[Category: Wallden, K]]		[[Category: Wallden, K]]

OCA at 09:35, 8 December 2014

2014-12-08T09:35:24Z

←Older revision		Revision as of 09:35, 8 December 2014
Line 1:		Line 1:
-	[[~~Image:~~2xjc.~~png~~\|~~left~~\|~~200px~~]]	+	==CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE==
		+	<StructureSection load='2xjc' size='340' side='right' caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xjc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xje\|2xje]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2xjb\|2xjb]], [[2jcm\|2jcm]], [[2xjf\|2xjf]], [[2xcx\|2xcx]], [[2xcw\|2xcw]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xjc RCSB], [http://www.ebi.ac.uk/pdbsum/2xjc PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytosolic 5'-nucleotidase II (cN-II) catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates and participates in the regulation of purine nucleotide pools within the cell. It interferes with the phosphorylation-dependent activation of nucleoside analogues used in the treatment of cancer and viral diseases. It is allosterically activated by a number of phosphate-containing cellular metabolites such as ATP, diadenosine polyphosphates, and 2,3-bisphosphoglycerate, which couple its activity with the metabolic state of the cell. We present seven high-resolution structures of human cN-II, including a ligand-free form and complexes with various substrates and effectors. These structures reveal the structural basis for the allosteric activation of cN-II, uncovering a mechanism where an effector-induced disorder-to-order transition generates rearrangements within the catalytic site and the subsequent coordination of the catalytically essential magnesium. Central to the activation is the large transition of the catalytically essential Asp356. This study also provides the structural basis for the substrate specificity of cN-II, where Arg202, Asp206, and Phe157 seem to be important residues for purine/pyrimidine selectivity. These structures provide a comprehensive structural basis for the design of cN-II inhibitors. They also contribute to the understanding of how the nucleotide salvage pathway is regulated at a molecular level.

-	~~{{STRUCTURE_2xjc\| PDB=2xjc \| SCENE= }}~~	+	Structural Basis for the Allosteric Regulation and Substrate Recognition of Human Cytosolic 5'-Nucleotidase II.,Wallden K, Nordlund P J Mol Biol. 2011 May 13;408(4):684-96. Epub 2011 Mar 17. PMID:21396942<ref>PMID:21396942</ref>

-	~~===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21396942}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2xjc]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021396942<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Nordlund, P.]]	+	[[Category: Nordlund, P]]
-	[[Category: Wallden, K.]]	+	[[Category: Wallden, K]]
	[[Category: Allosteric enzyme]]		[[Category: Allosteric enzyme]]
	[[Category: Cn-ii]]		[[Category: Cn-ii]]

OCA at 14:59, 6 March 2013

2013-03-06T14:59:14Z

←Older revision		Revision as of 14:59, 6 March 2013
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	[[Image:2xjc.png\|left\|200px]]		[[Image:2xjc.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xjc", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2xjc\| PDB=2xjc \| SCENE= }}		{{STRUCTURE_2xjc\| PDB=2xjc \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE===		===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21396942}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21396942 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21396942}}		{{ABSTRACT_PUBMED_21396942}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21396942~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021396942</ref><references group="xtra"/>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Nordlund, P.]]		[[Category: Nordlund, P.]]
	[[Category: Wallden, K.]]		[[Category: Wallden, K.]]
		+	[[Category: Allosteric enzyme]]
		+	[[Category: Cn-ii]]
		+	[[Category: Gmp-imp specific nucleotidase]]
		+	[[Category: High km 5-prime nucleotidase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Metal-binding]]
		+	[[Category: Nt5c2]]
		+	[[Category: Nucleotide metabolism]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Phosphoprotein]]

OCA at 06:30, 27 April 2011

2011-04-27T06:30:19Z

←Older revision		Revision as of 06:30, 27 April 2011
Line 1:		Line 1:
-	[[Image:2xjc.~~jpg~~\|left\|200px]]	+	[[Image:2xjc.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE===		===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21396942}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21396942 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21396942}}

	==About this Structure==		==About this Structure==
	[[2xjc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA].		[[2xjc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21396942</ref><references group="xtra"/>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Nordlund, P.]]		[[Category: Nordlund, P.]]
	[[Category: Wallden, K.]]		[[Category: Wallden, K.]]

OCA at 08:27, 16 March 2011

2011-03-16T08:27:12Z

←Older revision		Revision as of 08:27, 16 March 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2xjc.jpg\|left\|200px]]

-	The ~~entry 2xjc~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2xjc", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2xjc\| PDB=2xjc \| SCENE= }}

-	~~Authors: Wallden, K., Nordlund, P.~~	+	===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II IN COMPLEX WITH GUANOSINE MONOPHOSPHATE AND DIADENOSINE TETRAPHOSPHATE===

-	~~Description: Crystal~~ structure ~~of the D52N variant of cytosolic~~ 5'-nucleotidase ~~II in complex with guanosine monophosphate and diadenosine tetraphosphate~~	+
		+	==About this Structure==
		+	[[2xjc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA].
		+	[[Category: 5'-nucleotidase]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Nordlund, P.]]
		+	[[Category: Wallden, K.]]

OCA: Protected "2xjc" [edit=sysop:move=sysop]

2011-01-12T04:40:11Z

Protected "2xjc" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:40, 12 January 2011

←Older revision		Revision as of 10:54, 13 April 2022
Line 3:		Line 3:
	<StructureSection load='2xjc' size='340' side='right'caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2xjc' size='340' side='right'caption='[[2xjc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xje\|2xje]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2xjb\|2xjb]], [[2jcm\|2jcm]], [[2xjf\|2xjf]], [[2xcx\|2xcx]], [[2xcw\|2xcw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xje\|2xje]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2xjb\|2xjb]], [[2jcm\|2jcm]], [[2xjf\|2xjf]], [[2xcx\|2xcx]], [[2xcw\|2xcw]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjc OCA], [~~http~~://pdbe.org/2xjc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xjc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xjc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjc OCA], [https://pdbe.org/2xjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xjc RCSB], [https://www.ebi.ac.uk/pdbsum/2xjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjc ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>	+	[[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.	+	[[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==