2xlp - Revision history

OCA at 10:34, 20 December 2023

2023-12-20T10:34:00Z

←Older revision		Revision as of 10:34, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2xlp' size='340' side='right'caption='[[2xlp]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2xlp' size='340' side='right'caption='[[2xlp]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xlp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Jcm_14647 Jcm 14647~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xlp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylophaga_aminisulfidivorans Methylophaga aminisulfidivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XLP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vqb\|2vqb]], [[2vq7\|2vq7]], [[2xlr\|2xlr]], [[2xls\|2xls]], [[2xlu\|2xlu]], [[2xlt\|2xlt]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Flavin-containing_monooxygenase Flavin-containing monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.8 1.14.13.8] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlp OCA], [https://pdbe.org/2xlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xlp RCSB], [https://www.ebi.ac.uk/pdbsum/2xlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xlp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlp OCA], [https://pdbe.org/2xlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xlp RCSB], [https://www.ebi.ac.uk/pdbsum/2xlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xlp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q83XK4_9GAMM Q83XK4_9GAMM]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Flavin-containing monooxygenase]]
-	[[Category: Jcm 14647]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Fraaije, M W~~]]	+	[[Category: Methylophaga aminisulfidivorans]]
-	[[Category: ~~Mattevi, A~~]]	+	[[Category: Fraaije MW]]
-	[[Category: ~~Orru, R~~]]	+	[[Category: Mattevi A]]
-	[[Category: ~~Oxidoreductase]]~~	+	[[Category: Orru R]]
-	~~[[Category: Trimethyaminuria~~]]	+

OCA at 14:51, 17 November 2021

2021-11-17T14:51:07Z

←Older revision		Revision as of 14:51, 17 November 2021
Line 1:		Line 1:

	==Joint-functions of protein residues and NADP(H) in oxygen-activation by flavin-containing monooxygenase: Asn78Ser mutant==		==Joint-functions of protein residues and NADP(H) in oxygen-activation by flavin-containing monooxygenase: Asn78Ser mutant==
-	<StructureSection load='2xlp' size='340' side='right' caption='[[2xlp]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='2xlp' size='340' side='right'caption='[[2xlp]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xlp]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Jcm_14647 Jcm 14647]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xlp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Jcm_14647 Jcm 14647]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XLP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vqb\|2vqb]], [[2vq7\|2vq7]], [[2xlr\|2xlr]], [[2xls\|2xls]], [[2xlu\|2xlu]], [[2xlt\|2xlt]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vqb\|2vqb]], [[2vq7\|2vq7]], [[2xlr\|2xlr]], [[2xls\|2xls]], [[2xlu\|2xlu]], [[2xlt\|2xlt]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Flavin-containing_monooxygenase Flavin-containing monooxygenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.8 1.14.13.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Flavin-containing_monooxygenase Flavin-containing monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.8 1.14.13.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlp OCA], [~~http~~://pdbe.org/2xlp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xlp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xlp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xlp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlp OCA], [https://pdbe.org/2xlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xlp RCSB], [https://www.ebi.ac.uk/pdbsum/2xlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xlp ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Monooxygenase\|Monooxygenase]]	+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	[[Category: Flavin-containing monooxygenase]]		[[Category: Flavin-containing monooxygenase]]
	[[Category: Jcm 14647]]		[[Category: Jcm 14647]]
		+	[[Category: Large Structures]]
	[[Category: Fraaije, M W]]		[[Category: Fraaije, M W]]
	[[Category: Mattevi, A]]		[[Category: Mattevi, A]]

OCA at 09:51, 3 October 2018

2018-10-03T09:51:36Z

←Older revision		Revision as of 09:51, 3 October 2018
Line 1:		Line 1:

-	==~~JOINT~~-~~FUNCTIONS OF PROTEIN RESIDUES AND~~ NADP(H) ~~IN OXYGEN~~-~~ACTIVATION BY FLAVIN~~-~~CONTAINING MONOOXYGENASE~~: ~~ASN78SER MUTANT~~==	+	==Joint-functions of protein residues and NADP(H) in oxygen-activation by flavin-containing monooxygenase: Asn78Ser mutant==
	<StructureSection load='2xlp' size='340' side='right' caption='[[2xlp]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2xlp' size='340' side='right' caption='[[2xlp]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xl/2xlp_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xl/2xlp_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 2xlp" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2xlp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase\|Monooxygenase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:47, 4 August 2016

2016-08-04T23:47:38Z

←Older revision		Revision as of 23:47, 4 August 2016
Line 1:		Line 1:
		+
	==JOINT-FUNCTIONS OF PROTEIN RESIDUES AND NADP(H) IN OXYGEN-ACTIVATION BY FLAVIN-CONTAINING MONOOXYGENASE: ASN78SER MUTANT==		==JOINT-FUNCTIONS OF PROTEIN RESIDUES AND NADP(H) IN OXYGEN-ACTIVATION BY FLAVIN-CONTAINING MONOOXYGENASE: ASN78SER MUTANT==
	<StructureSection load='2xlp' size='340' side='right' caption='[[2xlp]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2xlp' size='340' side='right' caption='[[2xlp]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xlp]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Methylophaga_aminisulfidivorans Methylophaga aminisulfidivorans~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xlp]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Jcm_14647 Jcm 14647]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XLP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vqb\|2vqb]], [[2vq7\|2vq7]], [[2xlr\|2xlr]], [[2xls\|2xls]], [[2xlu\|2xlu]], [[2xlt\|2xlt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vqb\|2vqb]], [[2vq7\|2vq7]], [[2xlr\|2xlr]], [[2xls\|2xls]], [[2xlu\|2xlu]], [[2xlt\|2xlt]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Flavin-containing_monooxygenase Flavin-containing monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.8 1.14.13.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Flavin-containing_monooxygenase Flavin-containing monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.8 1.14.13.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xlp RCSB], [http://www.ebi.ac.uk/pdbsum/2xlp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlp OCA], [http://pdbe.org/2xlp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xlp RCSB], [http://www.ebi.ac.uk/pdbsum/2xlp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xlp ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xlp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xlp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Flavin-containing monooxygenase]]		[[Category: Flavin-containing monooxygenase]]
-	[[Category: ~~Methylophaga aminisulfidivorans~~]]	+	[[Category: Jcm 14647]]
	[[Category: Fraaije, M W]]		[[Category: Fraaije, M W]]
	[[Category: Mattevi, A]]		[[Category: Mattevi, A]]

OCA at 20:51, 24 December 2014

2014-12-24T20:51:33Z

←Older revision		Revision as of 20:51, 24 December 2014
Line 32:		Line 32:
	[[Category: Flavin-containing monooxygenase]]		[[Category: Flavin-containing monooxygenase]]
	[[Category: Methylophaga aminisulfidivorans]]		[[Category: Methylophaga aminisulfidivorans]]
-	[[Category: Fraaije, M W.]]	+	[[Category: Fraaije, M W]]
-	[[Category: Mattevi, A.]]	+	[[Category: Mattevi, A]]
-	[[Category: Orru, R.]]	+	[[Category: Orru, R]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Trimethyaminuria]]		[[Category: Trimethyaminuria]]

OCA at 13:22, 22 October 2014

2014-10-22T13:22:56Z

←Older revision		Revision as of 13:22, 22 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2xlp.~~png~~\|~~left~~\|200px]]	+	==JOINT-FUNCTIONS OF PROTEIN RESIDUES AND NADP(H) IN OXYGEN-ACTIVATION BY FLAVIN-CONTAINING MONOOXYGENASE: ASN78SER MUTANT==
		+	<StructureSection load='2xlp' size='340' side='right' caption='[[2xlp]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xlp]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylophaga_aminisulfidivorans Methylophaga aminisulfidivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XLP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vqb\|2vqb]], [[2vq7\|2vq7]], [[2xlr\|2xlr]], [[2xls\|2xls]], [[2xlu\|2xlu]], [[2xlt\|2xlt]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Flavin-containing_monooxygenase Flavin-containing monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.8 1.14.13.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xlp RCSB], [http://www.ebi.ac.uk/pdbsum/2xlp PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xl/2xlp_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The reactivity of flavoenzymes with dioxygen is at the heart of a number of biochemical reactions with far reaching implications for cell physiology and pathology. Flavin-containing monooxygenases are an attractive model system to study flavin-mediated oxygenation. In these enzymes, the NADP(H) cofactor is essential for stabilizing the flavin intermediate which activates dioxygen and makes it ready to react with the substrate undergoing oxygenation. Our studies combine site-directed mutagenesis with the usage of NADP+ analogues to dissect the specific roles of the cofactors and surrounding protein matrix. The highlight of this "double-engineering" approach is that subtle alterations in the hydrogen-bonding and stereochemical environment can drastically alter the efficiency and outcome of the reaction with oxygen. This is illustrated by the seemingly marginal replacement of an Asn to Ser in the oxygen-reacting site which inactivates the enzyme by effectively converting it into an oxidase. These data rationalize the effect of mutations that cause enzyme deficiency in patients affected by the fish odor syndrome. A crucial role of NADP+ in the oxygenation reaction is to shield the reacting flavin N5 atom by H-bond interactions. A Tyr residue functions as backdoor that stabilizes this crucial binding conformation of the nicotinamide cofactor. A general concept emerging from this analysis is that the two alternative pathways of flavoprotein-oxygen reactivity (oxidation vs monooxygenation) are predicted to have very similar activation barriers. The necessity of fine tuning the hydrogen-bonding, electrostatics, and accessibility of the flavin will represent a challenge, for the design and development of oxidases and monoxygenases for biotechnological applications.

-	~~{{STRUCTURE_2xlp\| PDB=2xlp \| SCENE= }}~~	+	Joint-functions of protein residues and NADP(H) in oxygen-activation by flavin-containing monooxygenase.,Orru R, Torres Pazmino DE, Fraaije MW, Mattevi A J Biol Chem. 2010 Aug 31. PMID:20807767<ref>PMID:20807767</ref>

-	~~===JOINT-FUNCTIONS OF PROTEIN RESIDUES AND NADP(H) IN OXYGEN-ACTIVATION BY FLAVIN-CONTAINING MONOOXYGENASE: ASN78SER MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20807767}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2xlp]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Methylophaga_aminisulfidivorans Methylophaga aminisulfidivorans]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020807767<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Flavin-containing monooxygenase]]		[[Category: Flavin-containing monooxygenase]]
	[[Category: Methylophaga aminisulfidivorans]]		[[Category: Methylophaga aminisulfidivorans]]

OCA: Protected "2xlp" [edit=sysop:move=sysop]

2013-01-07T21:47:52Z

Protected "2xlp" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:47, 7 January 2013

OCA at 21:47, 7 January 2013

2013-01-07T21:47:49Z

←Older revision		Revision as of 21:47, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2xlp.png\|left\|200px]]		[[Image:2xlp.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xlp", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2xlp\| PDB=2xlp \| SCENE= }}		{{STRUCTURE_2xlp\| PDB=2xlp \| SCENE= }}

	===JOINT-FUNCTIONS OF PROTEIN RESIDUES AND NADP(H) IN OXYGEN-ACTIVATION BY FLAVIN-CONTAINING MONOOXYGENASE: ASN78SER MUTANT===		===JOINT-FUNCTIONS OF PROTEIN RESIDUES AND NADP(H) IN OXYGEN-ACTIVATION BY FLAVIN-CONTAINING MONOOXYGENASE: ASN78SER MUTANT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20807767}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20807767 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20807767}}		{{ABSTRACT_PUBMED_20807767}}

	==About this Structure==		==About this Structure==
-	~~2XLP~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Methylophaga_aminisulfidivorans Methylophaga aminisulfidivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA].	+	[[2xlp]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylophaga_aminisulfidivorans Methylophaga aminisulfidivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20807767~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020807767</ref><references group="xtra"/>
	[[Category: Flavin-containing monooxygenase]]		[[Category: Flavin-containing monooxygenase]]
	[[Category: Methylophaga aminisulfidivorans]]		[[Category: Methylophaga aminisulfidivorans]]
Line 31:		Line 19:
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Trimethyaminuria]]		[[Category: Trimethyaminuria]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 10 06:54:14 2010''

OCA at 03:43, 10 November 2010

2010-11-10T03:43:19Z

←Older revision		Revision as of 03:43, 10 November 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:2xlp.~~jpg~~\|left\|200px]]	+	[[Image:2xlp.png\|left\|200px]]

	<!--		<!--
Line 32:		Line 32:
	[[Category: Trimethyaminuria]]		[[Category: Trimethyaminuria]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 15~~ 10:46:31 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 10 06:54:14 2010''

OCA at 07:37, 15 September 2010

2010-09-15T07:37:51Z

←Older revision		Revision as of 07:37, 15 September 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:2xlp.jpg\|left\|200px]]

-	The ~~entry 2xlp~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2xlp", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2xlp\| PDB=2xlp \| SCENE= }}

-	~~Authors~~: ~~Orru, R., Fraaije, M.W., Mattevi, A.~~	+	===JOINT-FUNCTIONS OF PROTEIN RESIDUES AND NADP(H) IN OXYGEN-ACTIVATION BY FLAVIN-CONTAINING MONOOXYGENASE: ASN78SER MUTANT===

-	Description: Joint-functions of protein residues and NADP(H) in oxygen-activation by flavin-containing monooxygenase: Asn78Ser mutant

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 28 12~~:19:28 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20807767}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20807767 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20807767}}
		+
		+	==About this Structure==
		+	2XLP is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Methylophaga_aminisulfidivorans Methylophaga aminisulfidivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLP OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20807767</ref><references group="xtra"/>
		+	[[Category: Flavin-containing monooxygenase]]
		+	[[Category: Methylophaga aminisulfidivorans]]
		+	[[Category: Fraaije, M W.]]
		+	[[Category: Mattevi, A.]]
		+	[[Category: Orru, R.]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Trimethyaminuria]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 15 10:46:31 2010''