2xtr - Revision history

OCA at 10:39, 20 December 2023

2023-12-20T10:39:05Z

←Older revision		Revision as of 10:39, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2xtr' size='340' side='right'caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='2xtr' size='340' side='right'caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xtr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XTR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xtr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XTR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.14Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2xmx\|2xmx]], [[2xtq\|2xtq]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [https://pdbe.org/2xtr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [https://www.ebi.ac.uk/pdbsum/2xtr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [https://pdbe.org/2xtr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [https://www.ebi.ac.uk/pdbsum/2xtr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CEAM_ECOLX CEAM_ECOLX]] Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. This is a calcium-requiring inhibitor for murein biosynthesis; it causes lysis of sensitive cells accompanied by murein degradation. The target site is possibly the cytoplasmic membrane.	+	[https://www.uniprot.org/uniprot/CEAM_ECOLX CEAM_ECOLX] Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. This is a calcium-requiring inhibitor for murein biosynthesis; it causes lysis of sensitive cells accompanied by murein degradation. The target site is possibly the cytoplasmic membrane.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Braun, V]]	+	[[Category: Braun V]]
-	[[Category: Helbig, S]]	+	[[Category: Helbig S]]
-	[[Category: Patzer~~, S I~~]]	+	[[Category: Patzer SI]]
-	[[Category: Zeth, K]]	+	[[Category: Zeth K]]
-	~~[[Category: Antimicrobial protein]]~~	+
-	~~[[Category: Bactericin]]~~	+
-	~~[[Category: Cma~~]]	+

OCA at 12:17, 27 April 2022

2022-04-27T12:17:14Z

←Older revision		Revision as of 12:17, 27 April 2022
Line 3:		Line 3:
	<StructureSection load='2xtr' size='340' side='right'caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='2xtr' size='340' side='right'caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xtr]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XTR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xtr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XTR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xmx\|2xmx]], [[2xtq\|2xtq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xmx\|2xmx]], [[2xtq\|2xtq]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [~~http~~://pdbe.org/2xtr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xtr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [https://pdbe.org/2xtr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [https://www.ebi.ac.uk/pdbsum/2xtr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CEAM_ECOLX CEAM_ECOLX]] Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. This is a calcium-requiring inhibitor for murein biosynthesis; it causes lysis of sensitive cells accompanied by murein degradation. The target site is possibly the cytoplasmic membrane.	+	[[https://www.uniprot.org/uniprot/CEAM_ECOLX CEAM_ECOLX]] Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. This is a calcium-requiring inhibitor for murein biosynthesis; it causes lysis of sensitive cells accompanied by murein degradation. The target site is possibly the cytoplasmic membrane.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:20, 22 July 2020

2020-07-22T12:20:49Z

←Older revision		Revision as of 12:20, 22 July 2020
Line 1:		Line 1:

	==Structure of the P176A Colicin M mutant from E. coli==		==Structure of the P176A Colicin M mutant from E. coli==
-	<StructureSection load='2xtr' size='340' side='right' caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>	+	<StructureSection load='2xtr' size='340' side='right'caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xtr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XTR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xtr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XTR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xmx\|2xmx]], [[2xtq\|2xtq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xmx\|2xmx]], [[2xtq\|2xtq]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [http://pdbe.org/2xtr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [http://www.ebi.ac.uk/pdbsum/2xtr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [http://pdbe.org/2xtr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [http://www.ebi.ac.uk/pdbsum/2xtr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Colicin\|Colicin]]	+	*[[Colicin 3D structures\|Colicin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Braun, V]]		[[Category: Braun, V]]
	[[Category: Helbig, S]]		[[Category: Helbig, S]]

OCA at 08:52, 10 October 2018

2018-10-10T08:52:51Z

←Older revision		Revision as of 08:52, 10 October 2018
Line 1:		Line 1:

-	==~~STRUCTURE OF THE~~ P176A ~~COLICIN~~ M ~~MUTANT FROM~~ E. ~~COLI~~==	+	==Structure of the P176A Colicin M mutant from E. coli==
	<StructureSection load='2xtr' size='340' side='right' caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='2xtr' size='340' side='right' caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 02:39, 5 August 2016

2016-08-05T02:39:37Z

←Older revision		Revision as of 02:39, 5 August 2016
Line 1:		Line 1:
		+
	==STRUCTURE OF THE P176A COLICIN M MUTANT FROM E. COLI==		==STRUCTURE OF THE P176A COLICIN M MUTANT FROM E. COLI==
	<StructureSection load='2xtr' size='340' side='right' caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='2xtr' size='340' side='right' caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xtr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XTR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xtr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XTR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xmx\|2xmx]], [[2xtq\|2xtq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xmx\|2xmx]], [[2xtq\|2xtq]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [http://www.ebi.ac.uk/pdbsum/2xtr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [http://pdbe.org/2xtr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [http://www.ebi.ac.uk/pdbsum/2xtr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xtr" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Braun, V]]		[[Category: Braun, V]]
	[[Category: Helbig, S]]		[[Category: Helbig, S]]

OCA at 15:11, 25 December 2014

2014-12-25T15:11:28Z

←Older revision		Revision as of 15:11, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [http://www.ebi.ac.uk/pdbsum/2xtr PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [http://www.ebi.ac.uk/pdbsum/2xtr PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CEAM_ECOLX CEAM_ECOLX]] Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. This is a calcium-requiring inhibitor for murein biosynthesis; it causes lysis of sensitive cells accompanied by murein degradation. The target site is possibly the cytoplasmic membrane.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Braun, V.]]	+	[[Category: Braun, V]]
-	[[Category: Helbig, S.]]	+	[[Category: Helbig, S]]
-	[[Category: Patzer, S I.]]	+	[[Category: Patzer, S I]]
-	[[Category: Zeth, K.]]	+	[[Category: Zeth, K]]
	[[Category: Antimicrobial protein]]		[[Category: Antimicrobial protein]]
	[[Category: Bactericin]]		[[Category: Bactericin]]
	[[Category: Cma]]		[[Category: Cma]]

OCA at 12:57, 22 October 2014

2014-10-22T12:57:25Z

←Older revision		Revision as of 12:57, 22 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2xtr.~~png~~\|~~left~~\|~~200px~~]]	+	==STRUCTURE OF THE P176A COLICIN M MUTANT FROM E. COLI==
		+	<StructureSection load='2xtr' size='340' side='right' caption='[[2xtr]], [[Resolution\|resolution]] 2.14Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xtr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XTR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xmx\|2xmx]], [[2xtq\|2xtq]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xtr RCSB], [http://www.ebi.ac.uk/pdbsum/2xtr PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Colicin M (Cma) is specifically imported into the periplasm of Escherichia coli and kills the cells. Killing depends on the periplasmic peptidyl prolyl cis-trans isomerase (PPIase)/chaperone FkpA. To identify the Cma prolyl bonds targeted by FkpA, we replaced the 15 proline residues individually with alanine. Nine mutant proteins were fully active; Cma(P129A), Cma(P176A), and Cma(P260A) displayed 1% and Cma(P107A) 10% of the wild-type activity. Cma(P107A), Cma(P129A), and Cma(P260A) but not Cma(P176A) killed cells after entering the periplasm via osmotic shock, indicating that the former mutants were translocation deficient; Cma(P129A) did not bind to the FhuA outer membrane receptor. The crystal structures of Cma and Cma(P176A) were identical, excluding inactivation of the activity domain located far from P176. In a new PPIase assay, FkpA isomerized the Cma prolyl bond in peptide FP(176) at a high rate but KP(107) and LP(260) at only <10% of that rate. The four mutant proteins secreted into the periplasm via a fused signal sequence were toxic but much less than wild-type Cma. Wild-type and mutant Cma proteins secreted or translocated across the outer membrane by energy-coupled import or unspecific osmotic shock were only active in the presence of FkpA. We propose that Cma unfolds during transfer across the outer or cytoplasmic membrane and refolds to the active form in the periplasm assisted by FkpA. Weak refolding of Cma(P176A) would explain its low activity in all assays. Of the four proline residues identified as being important for Cma activity, FP(176) is most likely targeted by FkpA.

-	~~{{STRUCTURE_2xtr\| PDB=2xtr \| SCENE= }}~~	+	Activation of colicin M by the FkpA prolyl cis-trans isomerase/chaperone.,Helbig S, Patzer SI, Schiene-Fischer C, Zeth K, Braun V J Biol Chem. 2010 Dec 13. PMID:21149455<ref>PMID:21149455</ref>

-	~~===STRUCTURE OF THE P176A COLICIN M MUTANT FROM E. COLI===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21149455}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2xtr]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA].~~	+

	==See Also==		==See Also==
	*[[Colicin\|Colicin]]		*[[Colicin\|Colicin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021149455<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Braun, V.]]		[[Category: Braun, V.]]

OCA at 18:10, 20 October 2012

2012-10-20T18:10:48Z

←Older revision		Revision as of 18:10, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[2xtr]] is a 2 chain structure ~~of [[Colicin]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA].	+	[[2xtr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA].

	==See Also==		==See Also==

OCA at 03:15, 27 July 2012

2012-07-27T03:15:56Z

←Older revision		Revision as of 03:15, 27 July 2012
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	[[Image:2xtr.png\|left\|200px]]		[[Image:2xtr.png\|left\|200px]]

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	{{STRUCTURE_2xtr\| PDB=2xtr \| SCENE= }}		{{STRUCTURE_2xtr\| PDB=2xtr \| SCENE= }}

	===STRUCTURE OF THE P176A COLICIN M MUTANT FROM E. COLI===		===STRUCTURE OF THE P176A COLICIN M MUTANT FROM E. COLI===

-
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	{{ABSTRACT_PUBMED_21149455}}		{{ABSTRACT_PUBMED_21149455}}

	==About this Structure==		==About this Structure==
-	[[2xtr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA].	+	[[2xtr]] is a 2 chain structure of [[Colicin]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTR OCA].
		+
		+	==See Also==
		+	*[[Colicin\|Colicin]]

	==Reference==		==Reference==
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	[[Category: Patzer, S I.]]		[[Category: Patzer, S I.]]
	[[Category: Zeth, K.]]		[[Category: Zeth, K.]]
		+	[[Category: Antimicrobial protein]]
		+	[[Category: Bactericin]]
		+	[[Category: Cma]]

OCA: Protected "2xtr" [edit=sysop:move=sysop]

2011-05-12T04:04:46Z

Protected "2xtr" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:04, 12 May 2011