2xuu - Revision history

OCA at 10:39, 20 December 2023

2023-12-20T10:39:32Z

←Older revision		Revision as of 10:39, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2xuu' size='340' side='right'caption='[[2xuu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2xuu' size='340' side='right'caption='[[2xuu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xuu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XUU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xuu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XUU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE~~-~~5-DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [https://pdbe.org/2xuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [https://www.ebi.ac.uk/pdbsum/2xuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xuu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [https://pdbe.org/2xuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [https://www.ebi.ac.uk/pdbsum/2xuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xuu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DAPK1_HUMAN DAPK1_HUMAN]] Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref> Isoform 2 cannot induce apoptosis but can induce membrane blebbing.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref>	+	[https://www.uniprot.org/uniprot/DAPK1_HUMAN DAPK1_HUMAN] Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref> Isoform 2 cannot induce apoptosis but can induce membrane blebbing.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Non-specific serine/threonine protein kinase]]~~	+	[[Category: Kuper J]]
-	~~[[Category: Diego, I de]]~~	+	[[Category: Lehmann F]]
-	[[Category: Kuper, J]]	+	[[Category: Wilmanns M]]
-	[[Category: Lehmann, F]]	+	[[Category: De Diego I]]
-	[[Category: Wilmanns, M]]	+
-	[[Category: ~~Apoptosis]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:17, 27 April 2022

2022-04-27T12:17:36Z

←Older revision		Revision as of 12:17, 27 April 2022
Line 3:		Line 3:
	<StructureSection load='2xuu' size='340' side='right'caption='[[2xuu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2xuu' size='340' side='right'caption='[[2xuu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xuu]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XUU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xuu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XUU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [~~http~~://pdbe.org/2xuu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xuu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xuu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [https://pdbe.org/2xuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [https://www.ebi.ac.uk/pdbsum/2xuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xuu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DAPK1_HUMAN DAPK1_HUMAN]] Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref> Isoform 2 cannot induce apoptosis but can induce membrane blebbing.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref>	+	[[https://www.uniprot.org/uniprot/DAPK1_HUMAN DAPK1_HUMAN]] Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref> Isoform 2 cannot induce apoptosis but can induce membrane blebbing.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:21, 22 July 2020

2020-07-22T12:21:45Z

←Older revision		Revision as of 12:21, 22 July 2020
Line 1:		Line 1:

	==Crystal structure of a DAP-kinase 1 mutant==		==Crystal structure of a DAP-kinase 1 mutant==
-	<StructureSection load='2xuu' size='340' side='right' caption='[[2xuu]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='2xuu' size='340' side='right'caption='[[2xuu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XUU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XUU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [http://pdbe.org/2xuu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [http://www.ebi.ac.uk/pdbsum/2xuu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xuu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [http://pdbe.org/2xuu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [http://www.ebi.ac.uk/pdbsum/2xuu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xuu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Death-associated protein kinase\|Death-associated protein kinase]]	+	*[[Death-associated protein kinase 3D structures\|Death-associated protein kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Diego, I de]]		[[Category: Diego, I de]]

OCA at 13:13, 4 August 2016

2016-08-04T13:13:14Z

←Older revision		Revision as of 13:13, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of a DAP-kinase 1 mutant==		==Crystal structure of a DAP-kinase 1 mutant==
	<StructureSection load='2xuu' size='340' side='right' caption='[[2xuu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2xuu' size='340' side='right' caption='[[2xuu]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [http://www.ebi.ac.uk/pdbsum/2xuu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [http://pdbe.org/2xuu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [http://www.ebi.ac.uk/pdbsum/2xuu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xuu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xuu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 09:37, 25 December 2014

2014-12-25T09:37:19Z

←Older revision		Revision as of 09:37, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XUU FirstGlance]. <br>		<table><tr><td colspan='2'>[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XUU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [http://www.ebi.ac.uk/pdbsum/2xuu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [http://www.ebi.ac.uk/pdbsum/2xuu PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DAPK1_HUMAN DAPK1_HUMAN]] Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref> Isoform 2 cannot induce apoptosis but can induce membrane blebbing.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	A PEF/Y Substrate Recognition and Signature Motif Plays a Critical Role in DAPK-Related Kinase Activity.,Temmerman K, de Diego I, Pogenberg V, Simon B, Jonko W, Li X, Wilmanns M Chem Biol. 2014 Jan 14. pii: S1074-5521(13)00457-2. doi:, 10.1016/j.chembiol.2013.12.008. PMID:24440081<ref>PMID:24440081</ref>		A PEF/Y Substrate Recognition and Signature Motif Plays a Critical Role in DAPK-Related Kinase Activity.,Temmerman K, de Diego I, Pogenberg V, Simon B, Jonko W, Li X, Wilmanns M Chem Biol. 2014 Jan 14. pii: S1074-5521(13)00457-2. doi:, 10.1016/j.chembiol.2013.12.008. PMID:24440081<ref>PMID:24440081</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Death-associated protein kinase\|Death-associated protein kinase]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 27:
	[[Category: Human]]		[[Category: Human]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
-	[[Category: Diego, I de.]]	+	[[Category: Diego, I de]]
-	[[Category: Kuper, J.]]	+	[[Category: Kuper, J]]
-	[[Category: Lehmann, F.]]	+	[[Category: Lehmann, F]]
-	[[Category: Wilmanns, M.]]	+	[[Category: Wilmanns, M]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 12:55, 18 May 2014

2014-05-18T12:55:49Z

←Older revision		Revision as of 12:55, 18 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2xuu\| PDB~~=2xuu \| ~~SCENE~~= }}	+	==Crystal structure of a DAP-kinase 1 mutant==
-	===~~Crystal~~ structure of a ~~DAP~~-kinase 1 ~~mutant~~===	+	<StructureSection load='2xuu' size='340' side='right' caption='[[2xuu]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24440081}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XUU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jkk\|1jkk]], [[2yak\|2yak]], [[1p4f\|1p4f]], [[2y0a\|2y0a]], [[2w4j\|2w4j]], [[2x0g\|2x0g]], [[2y4v\|2y4v]], [[3zxt\|3zxt]], [[2w4k\|2w4k]], [[1jkt\|1jkt]], [[1ig1\|1ig1]], [[1jks\|1jks]], [[1jkl\|1jkl]], [[2xzs\|2xzs]], [[2y4p\|2y4p]], [[4b4l\|4b4l]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xuu RCSB], [http://www.ebi.ac.uk/pdbsum/2xuu PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Knowledge about protein kinase substrate preferences is biased toward residues immediately adjacent to the site of phosphorylation. By a combined structural, biochemical, and cellular approach, we have discovered an unexpected substrate recognition element with the consensus sequence PEF/Y in the tumor suppressor death-associated protein kinase 1. This motif can be effectively blocked by a specific pseudosubstrate-type interaction with an autoregulatory domain of this kinase. In this arrangement, the central PEF/Y glutamate interacts with a conserved arginine distant to the phosphorylation site in sequence and structure. We also demonstrate that the element is crucial for kinase activity regulation and substrate recognition. The PEF/Y motif distinguishes close death-associated protein kinase relatives from canonical calcium/calmodulin-dependent protein kinases. Insight into this signature and mode of action offers new opportunities to identify specific small molecule inhibitors in PEF/Y-containing protein kinases.

-	~~==Function==~~	+	A PEF/Y Substrate Recognition and Signature Motif Plays a Critical Role in DAPK-Related Kinase Activity.,Temmerman K, de Diego I, Pogenberg V, Simon B, Jonko W, Li X, Wilmanns M Chem Biol. 2014 Jan 14. pii: S1074-5521(13)00457-2. doi:, 10.1016/j.chembiol.2013.12.008. PMID:24440081<ref>PMID:24440081</ref>
-	~~[[http:~~/~~/www~~.~~uniprot.org/uniprot/DAPK1_HUMAN DAPK1_HUMAN]] Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival~~, ~~apoptosis~~, ~~and autophagy. Regulates both type~~ I ~~apoptotic and type II autophagic cell deaths signal~~, ~~depending on the cellular setting. The former is caspase-dependent~~, ~~while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity~~, ~~nuclear localization~~, ~~and cellular function. Phosphorylates TPM1~~, ~~enhancing stress fiber formation in endothelial cells~~. ~~Phosphorylates STX1A and significantly decreases its binding to STXBP1~~. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-~~TSC2 complex and stimulating mTORC1 activity in a growth factor~~-~~dependent pathway~~. ~~Phosphorylates RPS6~~, ~~MYL9 and DAPK3~~. ~~Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke~~. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. ~~Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition~~.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref> Isoform 2 cannot induce apoptosis but can induce membrane blebbing.~~<ref>~~PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:~~21408167~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~[[2xuu]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Human Human]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA].~~	+	</div>
-		+	== References ==
-	==~~See Also==~~	+	<references/>
-	*[[Death-associated protein kinase\|Death-associated protein kinase]]	+	__TOC__
-		+	</StructureSection>
-	~~==Reference~~==	+
-	~~<ref group="xtra">PMID:024440081</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+
	[[Category: Human]]		[[Category: Human]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]

OCA at 09:23, 5 February 2014

2014-02-05T09:23:31Z

←Older revision		Revision as of 09:23, 5 February 2014
Line 1:		Line 1:
-	[[Image:2xuu.png\|left\|200px]]
-
	{{STRUCTURE_2xuu\| PDB=2xuu \| SCENE= }}		{{STRUCTURE_2xuu\| PDB=2xuu \| SCENE= }}
		+	===Crystal structure of a DAP-kinase 1 mutant===
		+	{{ABSTRACT_PUBMED_24440081}}

-	==~~=CRYSTAL STRUCTURE OF A DAP-KINASE 1 MUTANT=~~==	+	==Function==
-		+	[[http://www.uniprot.org/uniprot/DAPK1_HUMAN DAPK1_HUMAN]] Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref> Isoform 2 cannot induce apoptosis but can induce membrane blebbing.<ref>PMID:7828849</ref> <ref>PMID:10629061</ref> <ref>PMID:11579085</ref> <ref>PMID:11980920</ref> <ref>PMID:12730201</ref> <ref>PMID:15367680</ref> <ref>PMID:17703233</ref> <ref>PMID:17895359</ref> <ref>PMID:18422656</ref> <ref>PMID:18195017</ref> <ref>PMID:18995835</ref> <ref>PMID:19180116</ref> <ref>PMID:18974095</ref> <ref>PMID:21497122</ref> <ref>PMID:21408167</ref>

	==About this Structure==		==About this Structure==
-	[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA].	+	[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA].

	==See Also==		==See Also==
	*[[Death-associated protein kinase\|Death-associated protein kinase]]		*[[Death-associated protein kinase\|Death-associated protein kinase]]
-	[[Category: ~~Homo sapiens~~]]	+
		+	==Reference==
		+	<ref group="xtra">PMID:024440081</ref><references group="xtra"/><references/>
		+	[[Category: Human]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
-	[[Category: Diego, I De.]]	+	[[Category: Diego, I de.]]
	[[Category: Kuper, J.]]		[[Category: Kuper, J.]]
	[[Category: Lehmann, F.]]		[[Category: Lehmann, F.]]

OCA at 15:10, 20 October 2012

2012-10-20T15:10:59Z

←Older revision		Revision as of 15:10, 20 October 2012
Line 1:		Line 1:
-	[[Image:2xuu.~~jpg~~\|left\|200px]]	+	[[Image:2xuu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xuu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2xuu\| PDB=2xuu \| SCENE= }}		{{STRUCTURE_2xuu\| PDB=2xuu \| SCENE= }}

Line 14:		Line 8:
	==About this Structure==		==About this Structure==
	[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA].		[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA].
		+
		+	==See Also==
		+	*[[Death-associated protein kinase\|Death-associated protein kinase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]

OCA: Protected "2xuu" [edit=sysop:move=sysop]

2011-11-02T08:27:51Z

Protected "2xuu" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:27, 2 November 2011

OCA at 08:27, 2 November 2011

2011-11-02T08:27:45Z

←Older revision		Revision as of 08:27, 2 November 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:2xuu.jpg\|left\|200px]]

-	The ~~entry 2xuu~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2xuu", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2xuu\| PDB=2xuu \| SCENE= }}

-	~~Authors: de Diego, I., Kuper, J., Lehmann, F., Wilmanns, M.~~	+	===CRYSTAL STRUCTURE OF A DAP-KINASE 1 MUTANT===

-	Description: Crystal structure of a DAP-kinase 1 mutant

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Nov 3 09~~:56:~~52 2010''~~	+	==About this Structure==
		+	[[2xuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XUU OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Non-specific serine/threonine protein kinase]]
		+	[[Category: Diego, I De.]]
		+	[[Category: Kuper, J.]]
		+	[[Category: Lehmann, F.]]
		+	[[Category: Wilmanns, M.]]
		+	[[Category: Apoptosis]]
		+	[[Category: Atp-binding]]
		+	[[Category: Transferase]]