2y8y - Revision history

OCA at 11:49, 1 February 2024

2024-02-01T11:49:20Z

←Older revision		Revision as of 11:49, 1 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [https://pdbe.org/2y8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [https://www.ebi.ac.uk/pdbsum/2y8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8y ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.44Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [https://pdbe.org/2y8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [https://www.ebi.ac.uk/pdbsum/2y8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 07:27, 3 November 2022

2022-11-03T07:27:53Z

←Older revision		Revision as of 07:27, 3 November 2022
Line 3:		Line 3:
	<StructureSection load='2y8y' size='340' side='right'caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>		<StructureSection load='2y8y' size='340' side='right'caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wj9\|1wj9]], [[2y8w\|2y8w]], [[2y9h\|2y9h]]</div></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [https://pdbe.org/2y8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [https://www.ebi.ac.uk/pdbsum/2y8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8y ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [https://pdbe.org/2y8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [https://www.ebi.ac.uk/pdbsum/2y8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8y ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CAS6_THET8 CAS6_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This enzyme processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product (PubMed:21572442). Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>	+	[https://www.uniprot.org/uniprot/CAS6_THET8 CAS6_THET8] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This enzyme processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product (PubMed:21572442). Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thet8~~]]	+	[[Category: Thermus thermophilus HB8]]
-	[[Category: Doudna~~, J A~~]]	+	[[Category: Doudna JA]]
-	[[Category: Jinek, M]]	+	[[Category: Jinek M]]
-	[[Category: Sashital~~, D G]]~~	+	[[Category: Sashital DG]]
-	~~[[Category: Ferredoxin-like]]~~	+
-	~~[[Category: Hydrolase-rna complex~~]]	+

OCA at 12:25, 27 April 2022

2022-04-27T12:25:20Z

←Older revision		Revision as of 12:25, 27 April 2022
Line 3:		Line 3:
	<StructureSection load='2y8y' size='340' side='right'caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>		<StructureSection load='2y8y' size='340' side='right'caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wj9\|1wj9]], [[2y8w\|2y8w]], [[2y9h\|2y9h]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wj9\|1wj9]], [[2y8w\|2y8w]], [[2y9h\|2y9h]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [~~http~~://pdbe.org/2y8y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2y8y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [https://pdbe.org/2y8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [https://www.ebi.ac.uk/pdbsum/2y8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CAS6_THET8 CAS6_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This enzyme processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product (PubMed:21572442). Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>	+	[[https://www.uniprot.org/uniprot/CAS6_THET8 CAS6_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This enzyme processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product (PubMed:21572442). Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:39, 22 July 2020

2020-07-22T12:39:48Z

←Older revision		Revision as of 12:39, 22 July 2020
Line 1:		Line 1:

	==Structure B of CRISPR endoribonuclease Cse3 bound to 19 nt RNA==		==Structure B of CRISPR endoribonuclease Cse3 bound to 19 nt RNA==
-	<StructureSection load='2y8y' size='340' side='right' caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>	+	<StructureSection load='2y8y' size='340' side='right'caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wj9\|1wj9]], [[2y8w\|2y8w]], [[2y9h\|2y9h]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wj9\|1wj9]], [[2y8w\|2y8w]], [[2y9h\|2y9h]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [http://pdbe.org/2y8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [http://www.ebi.ac.uk/pdbsum/2y8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [http://pdbe.org/2y8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [http://www.ebi.ac.uk/pdbsum/2y8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 21:
	==See Also==		==See Also==
	*[[CRISPR type I-E (Cascade)\|CRISPR type I-E (Cascade)]]		*[[CRISPR type I-E (Cascade)\|CRISPR type I-E (Cascade)]]
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Thet8]]		[[Category: Thet8]]
	[[Category: Doudna, J A]]		[[Category: Doudna, J A]]

OCA at 09:28, 10 October 2018

2018-10-10T09:28:34Z

←Older revision		Revision as of 09:28, 10 October 2018
Line 1:		Line 1:

-	==~~STRUCTURE~~ B OF CRISPR ~~ENDORIBONUCLEASE CSE3 BOUND TO~~ 19 NT RNA==	+	==Structure B of CRISPR endoribonuclease Cse3 bound to 19 nt RNA==
	<StructureSection load='2y8y' size='340' side='right' caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>		<StructureSection load='2y8y' size='340' side='right' caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 20:		Line 20:

	==See Also==		==See Also==
		+	*[[CRISPR type I-E (Cascade)\|CRISPR type I-E (Cascade)]]
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==

OCA at 14:13, 4 August 2016

2016-08-04T14:13:12Z

←Older revision		Revision as of 14:13, 4 August 2016
Line 1:		Line 1:
		+
	==STRUCTURE B OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 19 NT RNA==		==STRUCTURE B OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 19 NT RNA==
	<StructureSection load='2y8y' size='340' side='right' caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>		<StructureSection load='2y8y' size='340' side='right' caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus Thermus thermophilus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wj9\|1wj9]], [[2y8w\|2y8w]], [[2y9h\|2y9h]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wj9\|1wj9]], [[2y8w\|2y8w]], [[2y9h\|2y9h]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [http://www.ebi.ac.uk/pdbsum/2y8y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [http://pdbe.org/2y8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [http://www.ebi.ac.uk/pdbsum/2y8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q53WG9_THET8 Q53WG9_THET8~~]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). ~~Processes~~ pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product. Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>	+	[[http://www.uniprot.org/uniprot/CAS6_THET8 CAS6_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This enzyme processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product (PubMed:21572442). Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2y8y" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermus thermophilus~~]]	+	[[Category: Thet8]]
	[[Category: Doudna, J A]]		[[Category: Doudna, J A]]
	[[Category: Jinek, M]]		[[Category: Jinek, M]]

OCA at 10:03, 25 December 2014

2014-12-25T10:03:16Z

←Older revision		Revision as of 10:03, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [http://www.ebi.ac.uk/pdbsum/2y8y PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [http://www.ebi.ac.uk/pdbsum/2y8y PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q53WG9_THET8 Q53WG9_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product. Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:10, 18 December 2014

2014-12-18T14:10:52Z

←Older revision		Revision as of 14:10, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2y8y\| PDB=2y8y \| SCENE= }}~~	+	==STRUCTURE B OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 19 NT RNA==
-	===STRUCTURE B OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 19 NT RNA===	+	<StructureSection load='2y8y' size='340' side='right' caption='[[2y8y]], [[Resolution\|resolution]] 1.44Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21572442}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2y8y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y8Y FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wj9\|1wj9]], [[2y8w\|2y8w]], [[2y9h\|2y9h]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y8y RCSB], [http://www.ebi.ac.uk/pdbsum/2y8y PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clustered regularly interspaced short palindromic repeat (CRISPR) chromosomal loci found in prokaryotes provide an adaptive immune system against bacteriophages and plasmids. CRISPR-specific endoRNases produce short RNA molecules (crRNAs) from CRISPR transcripts, which harbor sequences complementary to invasive nucleic acid elements and ensure their selective targeting by CRISPR-associated (Cas) proteins. The extreme sequence divergence of CRISPR-specific endoRNases and their RNA substrates has obscured homology-based comparison of RNA recognition and cleavage mechanisms. Here, we show that Cse3 type CRISPR-specific endoRNases bind a hairpin structure and residues downstream of the cleavage site within the repetitive segment of cognate CRISPR RNA. Cocrystal structures of Cse3-RNA complexes reveal an RNA-induced conformational change in the enzyme active site that aligns the RNA strand for site-specific cleavage. These studies provide insight into a catalytically essential RNA recognition mechanism by a large class of CRISPR-related endoRNases.

-	~~==Function==~~	+	An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3.,Sashital DG, Jinek M, Doudna JA Nat Struct Mol Biol. 2011 Jun;18(6):680-7. Epub 2011 May 15. PMID:21572442<ref>PMID:21572442</ref>
-	~~[[http://www~~.~~uniprot.org/uniprot/Q53WG9_THET8 Q53WG9_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat)~~, ~~is an adaptive immune system that provides protection against mobile genetic elements (viruses~~, ~~transposable elements and conjugative plasmids)~~. ~~CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA~~ (~~crRNA~~)~~. Processes pre~~-~~crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product~~. ~~Generates a 2',3'-cyclic phosphodiester~~.~~<ref>~~PMID:21572442~~</ref>~~ <ref>PMID:~~21572444~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[2y8y]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermus_thermophilus Thermus thermophilus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021572442</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
-	[[Category: Doudna, J A.]]	+	[[Category: Doudna, J A]]
-	[[Category: Jinek, M.]]	+	[[Category: Jinek, M]]
-	[[Category: Sashital, D G.]]	+	[[Category: Sashital, D G]]
	[[Category: Ferredoxin-like]]		[[Category: Ferredoxin-like]]
	[[Category: Hydrolase-rna complex]]		[[Category: Hydrolase-rna complex]]

OCA at 20:59, 17 April 2013

2013-04-17T20:59:33Z

←Older revision		Revision as of 20:59, 17 April 2013
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-	[[Image:2y8y.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_2y8y", creates the "Structure Box" on the page.
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	{{STRUCTURE_2y8y\| PDB=2y8y \| SCENE= }}		{{STRUCTURE_2y8y\| PDB=2y8y \| SCENE= }}
-
	===STRUCTURE B OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 19 NT RNA===		===STRUCTURE B OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 19 NT RNA===
		+	{{ABSTRACT_PUBMED_21572442}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/Q53WG9_THET8 Q53WG9_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product. Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21572442}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~), ~~where 21572442~~ is the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_21572442}}~~	+

	==About this Structure==		==About this Structure==
Line 22:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:021572442</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021572442</ref><references group="xtra"/><references/>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Doudna, J A.]]		[[Category: Doudna, J A.]]

OCA at 05:38, 15 June 2011

2011-06-15T05:38:34Z

←Older revision		Revision as of 05:38, 15 June 2011
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-	[[Image:2y8y.~~jpg~~\|left\|200px]]	+	[[Image:2y8y.png\|left\|200px]]

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	===STRUCTURE B OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 19 NT RNA===		===STRUCTURE B OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 19 NT RNA===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21572442}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21572442 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21572442}}

	==About this Structure==		==About this Structure==
	[[2y8y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA].		[[2y8y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8Y OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021572442</ref><references group="xtra"/>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Doudna, J A.]]		[[Category: Doudna, J A.]]
	[[Category: Jinek, M.]]		[[Category: Jinek, M.]]
	[[Category: Sashital, D G.]]		[[Category: Sashital, D G.]]
		+	[[Category: Ferredoxin-like]]
		+	[[Category: Hydrolase-rna complex]]