2ycn - Revision history

OCA at 08:12, 23 August 2023

2023-08-23T08:12:25Z

←Older revision		Revision as of 08:12, 23 August 2023
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	<StructureSection load='2ycn' size='340' side='right'caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='2ycn' size='340' side='right'caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacterium_freundii"_braak_1928 "bacterium~~ freundii~~" braak 1928~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YCN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YCN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=P61:(2E)-3-(3-FLUORO-4-HYDROXYPHENYL)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC+ACID'>P61</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.04Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ez1\|2ez1]], [[2vlh\|2vlh]], [[2tpl\|2tpl]], [[2vlf\|2vlf]], [[2ez2\|2ez2]], [[1tpl\|1tpl]], [[2yct\|2yct]], [[2ycp\|2ycp]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=P61:(2E)-3-(3-FLUORO-4-HYDROXYPHENYL)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC+ACID'>P61</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tyrosine_phenol-lyase Tyrosine phenol-lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.2 4.1.99.2] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ycn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycn OCA], [https://pdbe.org/2ycn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ycn RCSB], [https://www.ebi.ac.uk/pdbsum/2ycn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ycn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ycn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycn OCA], [https://pdbe.org/2ycn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ycn RCSB], [https://www.ebi.ac.uk/pdbsum/2ycn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ycn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TPL_CITFR TPL_CITFR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacterium~~ freundii ~~braak 1928~~]]	+	[[Category: Citrobacter freundii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Tyrosine phenol-lyase]]~~	+	[[Category: Antson AA]]
-	[[Category: Antson~~, A A~~]]	+	[[Category: Demidkina TV]]
-	[[Category: Demidkina~~, T V~~]]	+	[[Category: Faleev NG]]
-	[[Category: Faleev~~, N G~~]]	+	[[Category: Matkovic-Calogovic D]]
-	[[Category: Matkovic-Calogovic, D]]	+	[[Category: Milic D]]
-	[[Category: Milic, D]]	+	[[Category: Phillips RS]]
-	[[Category: Phillips~~, R S]]~~	+
-	~~[[Category: Beta-elimination]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Pyridoxal 5'-phosphate dependent enzyme~~]]	+

OCA at 15:03, 17 November 2021

2021-11-17T15:03:36Z

←Older revision		Revision as of 15:03, 17 November 2021
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	<StructureSection load='2ycn' size='340' side='right'caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='2ycn' size='340' side='right'caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2YCN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YCN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=P61:(2E)-3-(3-FLUORO-4-HYDROXYPHENYL)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC+ACID'>P61</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=P61:(2E)-3-(3-FLUORO-4-HYDROXYPHENYL)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC+ACID'>P61</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ez1\|2ez1]], [[2vlh\|2vlh]], [[2tpl\|2tpl]], [[2vlf\|2vlf]], [[2ez2\|2ez2]], [[1tpl\|1tpl]], [[2yct\|2yct]], [[2ycp\|2ycp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ez1\|2ez1]], [[2vlh\|2vlh]], [[2tpl\|2tpl]], [[2vlf\|2vlf]], [[2ez2\|2ez2]], [[1tpl\|1tpl]], [[2yct\|2yct]], [[2ycp\|2ycp]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Tyrosine_phenol-lyase Tyrosine phenol-lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.2 4.1.99.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tyrosine_phenol-lyase Tyrosine phenol-lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.2 4.1.99.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ycn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycn OCA], [~~http~~://pdbe.org/2ycn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ycn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ycn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ycn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ycn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycn OCA], [https://pdbe.org/2ycn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ycn RCSB], [https://www.ebi.ac.uk/pdbsum/2ycn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ycn ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Tyrosinase\|Tyrosinase]]	+	*[[Tyrosinase 3D structures\|Tyrosinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:27, 10 May 2019

2019-05-10T12:27:46Z

←Older revision		Revision as of 12:27, 10 May 2019
Line 1:		Line 1:

	==Y71F mutant of tyrosine phenol-lyase from Citrobacter freundii in complex with quinonoid intermediate formed with 3-fluoro-L-tyrosine==		==Y71F mutant of tyrosine phenol-lyase from Citrobacter freundii in complex with quinonoid intermediate formed with 3-fluoro-L-tyrosine==
-	<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>	+	<StructureSection load='2ycn' size='340' side='right'caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YCN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YCN FirstGlance]. <br>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Bacterium freundii braak 1928]]		[[Category: Bacterium freundii braak 1928]]
		+	[[Category: Large Structures]]
	[[Category: Tyrosine phenol-lyase]]		[[Category: Tyrosine phenol-lyase]]
	[[Category: Antson, A A]]		[[Category: Antson, A A]]

OCA at 07:59, 6 March 2019

2019-03-06T07:59:44Z

←Older revision		Revision as of 07:59, 6 March 2019
Line 1:		Line 1:

	==Y71F mutant of tyrosine phenol-lyase from Citrobacter freundii in complex with quinonoid intermediate formed with 3-fluoro-L-tyrosine==		==Y71F mutant of tyrosine phenol-lyase from Citrobacter freundii in complex with quinonoid intermediate formed with 3-fluoro-L-tyrosine==
-	<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>	+	<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YCN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YCN FirstGlance]. <br>

OCA at 09:13, 10 October 2018

2018-10-10T09:13:13Z

←Older revision		Revision as of 09:13, 10 October 2018
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-	==Y71F ~~MUTANT OF TYROSINE PHENOL~~-~~LYASE FROM CITROBACTER FREUNDII IN COMPLEX WITH QUINONOID INTERMEDIATE FORMED WITH~~ 3-~~FLUORO~~-L-~~TYROSINE~~==	+	==Y71F mutant of tyrosine phenol-lyase from Citrobacter freundii in complex with quinonoid intermediate formed with 3-fluoro-L-tyrosine==
	<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 19:28, 4 August 2016

2016-08-04T19:28:59Z

←Older revision		Revision as of 19:28, 4 August 2016
Line 1:		Line 1:
		+
	==Y71F MUTANT OF TYROSINE PHENOL-LYASE FROM CITROBACTER FREUNDII IN COMPLEX WITH QUINONOID INTERMEDIATE FORMED WITH 3-FLUORO-L-TYROSINE==		==Y71F MUTANT OF TYROSINE PHENOL-LYASE FROM CITROBACTER FREUNDII IN COMPLEX WITH QUINONOID INTERMEDIATE FORMED WITH 3-FLUORO-L-TYROSINE==
	<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Citrobacter_freundii Citrobacter~~ freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YCN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YCN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=P61:(2E)-3-(3-FLUORO-4-HYDROXYPHENYL)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC+ACID'>P61</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=P61:(2E)-3-(3-FLUORO-4-HYDROXYPHENYL)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC+ACID'>P61</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ez1\|2ez1]], [[2vlh\|2vlh]], [[2tpl\|2tpl]], [[2vlf\|2vlf]], [[2ez2\|2ez2]], [[1tpl\|1tpl]], [[2yct\|2yct]], [[2ycp\|2ycp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ez1\|2ez1]], [[2vlh\|2vlh]], [[2tpl\|2tpl]], [[2vlf\|2vlf]], [[2ez2\|2ez2]], [[1tpl\|1tpl]], [[2yct\|2yct]], [[2ycp\|2ycp]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosine_phenol-lyase Tyrosine phenol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.2 4.1.99.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosine_phenol-lyase Tyrosine phenol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.2 4.1.99.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ycn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ycn RCSB], [http://www.ebi.ac.uk/pdbsum/2ycn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ycn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycn OCA], [http://pdbe.org/2ycn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ycn RCSB], [http://www.ebi.ac.uk/pdbsum/2ycn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ycn ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ycn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tyrosinase\|Tyrosinase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Citrobacter~~ freundii]]	+	[[Category: Bacterium freundii braak 1928]]
	[[Category: Tyrosine phenol-lyase]]		[[Category: Tyrosine phenol-lyase]]
	[[Category: Antson, A A]]		[[Category: Antson, A A]]

OCA at 21:37, 3 January 2015

2015-01-03T21:37:27Z

←Older revision		Revision as of 21:37, 3 January 2015
Line 2:		Line 2:
	<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. <br>	+	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YCN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=P61:(2E)-3-(3-FLUORO-4-HYDROXYPHENYL)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC+ACID'>P61</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=P61:(2E)-3-(3-FLUORO-4-HYDROXYPHENYL)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC+ACID'>P61</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ez1\|2ez1]], [[2vlh\|2vlh]], [[2tpl\|2tpl]], [[2vlf\|2vlf]], [[2ez2\|2ez2]], [[1tpl\|1tpl]], [[2yct\|2yct]], [[2ycp\|2ycp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ez1\|2ez1]], [[2vlh\|2vlh]], [[2tpl\|2tpl]], [[2vlf\|2vlf]], [[2ez2\|2ez2]], [[1tpl\|1tpl]], [[2yct\|2yct]], [[2ycp\|2ycp]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.7~~.1.2 ~~2.7~~.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosine_phenol-lyase Tyrosine phenol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.2 4.1.99.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ycn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ycn RCSB], [http://www.ebi.ac.uk/pdbsum/2ycn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ycn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ycn RCSB], [http://www.ebi.ac.uk/pdbsum/2ycn PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Crystallographic Snapshots of Tyrosine Phenol-lyase Show That Substrate Strain Plays a Role in C-C Bond Cleavage.,Milic D, Demidkina TV, Faleev NG, Phillips RS, Matkovic-Calogovic D, Antson AA J Am Chem Soc. 2011 Oct 19;133(41):16468-76. Epub 2011 Sep 27. PMID:21899319<ref>PMID:21899319</ref>		Crystallographic Snapshots of Tyrosine Phenol-lyase Show That Substrate Strain Plays a Role in C-C Bond Cleavage.,Milic D, Demidkina TV, Faleev NG, Phillips RS, Matkovic-Calogovic D, Antson AA J Am Chem Soc. 2011 Oct 19;133(41):16468-76. Epub 2011 Sep 27. PMID:21899319<ref>PMID:21899319</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 22:
	[[Category: Citrobacter freundii]]		[[Category: Citrobacter freundii]]
	[[Category: Tyrosine phenol-lyase]]		[[Category: Tyrosine phenol-lyase]]
-	[[Category: Antson, A A.]]	+	[[Category: Antson, A A]]
-	[[Category: Demidkina, T V.]]	+	[[Category: Demidkina, T V]]
-	[[Category: Faleev, N G.]]	+	[[Category: Faleev, N G]]
-	[[Category: Matkovic-Calogovic, D.]]	+	[[Category: Matkovic-Calogovic, D]]
-	[[Category: Milic, D.]]	+	[[Category: Milic, D]]
-	[[Category: Phillips, R S.]]	+	[[Category: Phillips, R S]]
	[[Category: Beta-elimination]]		[[Category: Beta-elimination]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Pyridoxal 5'-phosphate dependent enzyme]]		[[Category: Pyridoxal 5'-phosphate dependent enzyme]]

OCA at 07:52, 14 May 2014

2014-05-14T07:52:11Z

←Older revision		Revision as of 07:52, 14 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2ycn.~~png~~\|~~left~~\|~~200px~~]]	+	==Y71F MUTANT OF TYROSINE PHENOL-LYASE FROM CITROBACTER FREUNDII IN COMPLEX WITH QUINONOID INTERMEDIATE FORMED WITH 3-FLUORO-L-TYROSINE==
		+	<StructureSection load='2ycn' size='340' side='right' caption='[[2ycn]], [[Resolution\|resolution]] 2.04Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=P61:(2E)-3-(3-FLUORO-4-HYDROXYPHENYL)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC+ACID'>P61</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ez1\|2ez1]], [[2vlh\|2vlh]], [[2tpl\|2tpl]], [[2vlf\|2vlf]], [[2ez2\|2ez2]], [[1tpl\|1tpl]], [[2yct\|2yct]], [[2ycp\|2ycp]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ycn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ycn RCSB], [http://www.ebi.ac.uk/pdbsum/2ycn PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The key step in the enzymatic reaction catalyzed by tyrosine phenol-lyase (TPL) is reversible cleavage of the Cbeta-Cgamma bond of l-tyrosine. Here, we present X-ray structures for two enzymatic states that form just before and after the cleavage of the carbon-carbon bond. As for most other pyridoxal 5'-phosphate-dependent enzymes, the first state, a quinonoid intermediate, is central for the catalysis. We captured this relatively unstable intermediate in the crystalline state by introducing substitutions Y71F or F448H in Citrobacter freundii TPL and briefly soaking crystals of the mutant enzymes with a substrate 3-fluoro-l-tyrosine followed by flash-cooling. The X-ray structures, determined at approximately 2.0 A resolution, reveal two quinonoid geometries: "relaxed" in the open and "tense" in the closed state of the active site. The "tense" state is characterized by changes in enzyme contacts made with the substrate's phenolic moiety, which result in significantly strained conformation at Cbeta and Cgamma positions. We also captured, at 2.25 A resolution, the X-ray structure for the state just after the substrate's Cbeta-Cgamma bond cleavage by preparing the ternary complex between TPL, alanine quinonoid and pyridine N-oxide, which mimics the alpha-aminoacrylate intermediate with bound phenol. In this state, the enzyme-ligand contacts remain almost exactly the same as in the "tense" quinonoid, indicating that the strain induced by the closure of the active site facilitates elimination of phenol. Taken together, structural observations demonstrate that the enzyme serves not only to stabilize the transition state but also to destabilize the ground state.

-	<!--	+	Crystallographic Snapshots of Tyrosine Phenol-lyase Show That Substrate Strain Plays a Role in C-C Bond Cleavage.,Milic D, Demidkina TV, Faleev NG, Phillips RS, Matkovic-Calogovic D, Antson AA J Am Chem Soc. 2011 Oct 19;133(41):16468-76. Epub 2011 Sep 27. PMID:21899319<ref>PMID:21899319</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2ycn"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_2ycn\| PDB=2ycn \| SCENE= }}~~	+

-	~~===Y71F MUTANT OF TYROSINE PHENOL-LYASE FROM CITROBACTER FREUNDII IN COMPLEX WITH QUINONOID INTERMEDIATE FORMED WITH 3-FLUORO-L-TYROSINE===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21899319}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21899319 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21899319}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2ycn]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021899319<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Citrobacter freundii]]		[[Category: Citrobacter freundii]]
	[[Category: Tyrosine phenol-lyase]]		[[Category: Tyrosine phenol-lyase]]

OCA at 08:18, 30 October 2011

2011-10-30T08:18:53Z

←Older revision		Revision as of 08:18, 30 October 2011
Line 1:		Line 1:
-	[[Image:2ycn.~~jpg~~\|left\|200px]]	+	[[Image:2ycn.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Y71F MUTANT OF TYROSINE PHENOL-LYASE FROM CITROBACTER FREUNDII IN COMPLEX WITH QUINONOID INTERMEDIATE FORMED WITH 3-FLUORO-L-TYROSINE===		===Y71F MUTANT OF TYROSINE PHENOL-LYASE FROM CITROBACTER FREUNDII IN COMPLEX WITH QUINONOID INTERMEDIATE FORMED WITH 3-FLUORO-L-TYROSINE===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21899319}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21899319 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21899319}}

	==About this Structure==		==About this Structure==
	[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA].		[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021899319</ref><references group="xtra"/>
	[[Category: Citrobacter freundii]]		[[Category: Citrobacter freundii]]
	[[Category: Tyrosine phenol-lyase]]		[[Category: Tyrosine phenol-lyase]]

OCA at 06:31, 14 September 2011

2011-09-14T06:31:17Z

←Older revision		Revision as of 06:31, 14 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2ycn.jpg\|left\|200px]]

-	The ~~entry 2ycn~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2ycn", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2ycn\| PDB=2ycn \| SCENE= }}

-	~~Authors: Milic, D., Demidkina, T.V., Faleev, N.G., Phillips, R.S., Matkovic~~-~~Calogovic, D., Antson, A.A.~~	+	===Y71F MUTANT OF TYROSINE PHENOL-LYASE FROM CITROBACTER FREUNDII IN COMPLEX WITH QUINONOID INTERMEDIATE FORMED WITH 3-FLUORO-L-TYROSINE===

-	~~Description~~: ~~Y71F mutant of tyrosine phenol-lyase~~ from Citrobacter freundii ~~in complex with quinonoid intermediate formed with 3~~-~~fluoro~~-L-~~tyrosine~~	+
		+	==About this Structure==
		+	[[2ycn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCN OCA].
		+	[[Category: Citrobacter freundii]]
		+	[[Category: Tyrosine phenol-lyase]]
		+	[[Category: Antson, A A.]]
		+	[[Category: Demidkina, T V.]]
		+	[[Category: Faleev, N G.]]
		+	[[Category: Matkovic-Calogovic, D.]]
		+	[[Category: Milic, D.]]
		+	[[Category: Phillips, R S.]]
		+	[[Category: Beta-elimination]]
		+	[[Category: Lyase]]
		+	[[Category: Pyridoxal 5'-phosphate dependent enzyme]]