2z1p - Revision history

OCA at 13:55, 13 March 2024

2024-03-13T13:55:49Z

←Older revision		Revision as of 13:55, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='2z1p' size='340' side='right'caption='[[2z1p]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2z1p' size='340' side='right'caption='[[2z1p]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [~~http~~://pdbe.org/2z1p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2z1p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2z1p ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [https://pdbe.org/2z1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [https://www.ebi.ac.uk/pdbsum/2z1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z1p ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q836L2_ENTFA Q836L2_ENTFA]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z1p ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z1p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We have determined the crystal structure of the ligand binding segment of the Enterococcus faecalis collagen binding MSCRAMM ACE (microbial surface components recognizing adhesive matrix molecules adhesin of collagen from enterococci). This segment is composed of two subdomains, N(1) and N(2), each adopting an IgG-like fold and forming a putative collagen binding surface at the interface between the two subdomains. This structure is very similar to that recently reported for CNA, the collagen binding MSCRAMM of Staphylococcus aureus, for which a unique ligand binding mechanism called the Collagen Hug was proposed. We suggest that ACE binds collagen by a similar mechanism and present the first biochemical evidence for this binding model. Replacing residues in the putative collagen binding trench of ACE N(2) with Ala residues affected collagen binding. A closed conformation of ACE stabilized by an engineered disulfide bond is unable to bind collagen. Finally, the importance of the residues in the N(2) extension in stabilizing the MSCRAMM-ligand complex is demonstrated by selected point and truncation mutations.
-
-	The Enterococcus faecalis MSCRAMM ACE binds its ligand by the Collagen Hug model.,Liu Q, Ponnuraj K, Xu Y, Ganesh VK, Sillanpaa J, Murray BE, Narayana SV, Hook M J Biol Chem. 2007 Jul 6;282(27):19629-37. Epub 2007 Mar 28. PMID:17392280<ref>PMID:17392280</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2z1p" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Adhesin 3D structures\|Adhesin 3D structures]]		*[[Adhesin 3D structures\|Adhesin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterococcus ~~proteiformis thiercelin and jouhaud 1903~~]]	+	[[Category: Enterococcus faecalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ponnuraj, K]]	+	[[Category: Ponnuraj K]]
-	~~[[Category: Ace]]~~	+
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Collagen]]~~	+
-	~~[[Category: Enterococcus faecali~~]]	+

OCA at 11:56, 29 July 2020

2020-07-29T11:56:21Z

←Older revision		Revision as of 11:56, 29 July 2020
Line 1:		Line 1:

	==The Enterococcus faecalis MSCRAMM ACE binds its ligands by the collagen Hug Model==		==The Enterococcus faecalis MSCRAMM ACE binds its ligands by the collagen Hug Model==
-	<StructureSection load='2z1p' size='340' side='right' caption='[[2z1p]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='2z1p' size='340' side='right'caption='[[2z1p]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [http://pdbe.org/2z1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [http://www.ebi.ac.uk/pdbsum/2z1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2z1p ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [http://pdbe.org/2z1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [http://www.ebi.ac.uk/pdbsum/2z1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2z1p ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:

	==See Also==		==See Also==
-	*[[Adhesin\|Adhesin]]	+	*[[Adhesin 3D structures\|Adhesin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]		[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
		+	[[Category: Large Structures]]
	[[Category: Ponnuraj, K]]		[[Category: Ponnuraj, K]]
	[[Category: Ace]]		[[Category: Ace]]

OCA at 08:58, 17 October 2018

2018-10-17T08:58:09Z

←Older revision		Revision as of 08:58, 17 October 2018
Line 1:		Line 1:
		+
	==The Enterococcus faecalis MSCRAMM ACE binds its ligands by the collagen Hug Model==		==The Enterococcus faecalis MSCRAMM ACE binds its ligands by the collagen Hug Model==
	<StructureSection load='2z1p' size='340' side='right' caption='[[2z1p]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2z1p' size='340' side='right' caption='[[2z1p]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>		<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [http://pdbe.org/2z1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [http://www.ebi.ac.uk/pdbsum/2z1p PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [http://pdbe.org/2z1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [http://www.ebi.ac.uk/pdbsum/2z1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2z1p ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 9:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z1/2z1p_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z1/2z1p_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 24:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 2z1p" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2z1p" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Adhesin\|Adhesin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:29, 7 February 2016

2016-02-07T10:29:40Z

←Older revision		Revision as of 10:29, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='2z1p' size='340' side='right' caption='[[2z1p]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2z1p' size='340' side='right' caption='[[2z1p]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterococcus_faecalis Enterococcus faecalis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [http://www.ebi.ac.uk/pdbsum/2z1p PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [http://pdbe.org/2z1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [http://www.ebi.ac.uk/pdbsum/2z1p PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z1p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2z1p" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterococcus ~~faecalis~~]]	+	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
	[[Category: Ponnuraj, K]]		[[Category: Ponnuraj, K]]
	[[Category: Ace]]		[[Category: Ace]]

OCA at 07:51, 20 January 2015

2015-01-20T07:51:51Z

←Older revision		Revision as of 07:51, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>		<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [http://www.ebi.ac.uk/pdbsum/2z1p PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [http://www.ebi.ac.uk/pdbsum/2z1p PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Enterococcus faecalis]]		[[Category: Enterococcus faecalis]]
-	[[Category: Ponnuraj, K.]]	+	[[Category: Ponnuraj, K]]
	[[Category: Ace]]		[[Category: Ace]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Collagen]]		[[Category: Collagen]]
	[[Category: Enterococcus faecali]]		[[Category: Enterococcus faecali]]

OCA at 04:51, 2 October 2014

2014-10-02T04:51:12Z

←Older revision		Revision as of 04:51, 2 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2z1p.~~png\|left~~\|200px]]	+	==The Enterococcus faecalis MSCRAMM ACE binds its ligands by the collagen Hug Model==
		+	<StructureSection load='2z1p' size='340' side='right' caption='[[2z1p]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z1P FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2z1p RCSB], [http://www.ebi.ac.uk/pdbsum/2z1p PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z1/2z1p_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have determined the crystal structure of the ligand binding segment of the Enterococcus faecalis collagen binding MSCRAMM ACE (microbial surface components recognizing adhesive matrix molecules adhesin of collagen from enterococci). This segment is composed of two subdomains, N(1) and N(2), each adopting an IgG-like fold and forming a putative collagen binding surface at the interface between the two subdomains. This structure is very similar to that recently reported for CNA, the collagen binding MSCRAMM of Staphylococcus aureus, for which a unique ligand binding mechanism called the Collagen Hug was proposed. We suggest that ACE binds collagen by a similar mechanism and present the first biochemical evidence for this binding model. Replacing residues in the putative collagen binding trench of ACE N(2) with Ala residues affected collagen binding. A closed conformation of ACE stabilized by an engineered disulfide bond is unable to bind collagen. Finally, the importance of the residues in the N(2) extension in stabilizing the MSCRAMM-ligand complex is demonstrated by selected point and truncation mutations.

-	~~{{STRUCTURE_2z1p\| PDB=2z1p \| SCENE= }}~~	+	The Enterococcus faecalis MSCRAMM ACE binds its ligand by the Collagen Hug model.,Liu Q, Ponnuraj K, Xu Y, Ganesh VK, Sillanpaa J, Murray BE, Narayana SV, Hook M J Biol Chem. 2007 Jul 6;282(27):19629-37. Epub 2007 Mar 28. PMID:17392280<ref>PMID:17392280</ref>

-	~~===The Enterococcus faecalis MSCRAMM ACE binds its ligands by~~ the ~~collagen Hug Model===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17392280}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2z1p]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterococcus_faecalis Enterococcus faecalis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017392280<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterococcus faecalis]]		[[Category: Enterococcus faecalis]]
	[[Category: Ponnuraj, K.]]		[[Category: Ponnuraj, K.]]

OCA: Protected "2z1p" [edit=sysop:move=sysop]

2013-01-07T20:42:59Z

Protected "2z1p" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:42, 7 January 2013

OCA at 20:42, 7 January 2013

2013-01-07T20:42:56Z

←Older revision		Revision as of 20:42, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2z1p.png\|left\|200px]]		[[Image:2z1p.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2z1p", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2z1p\| PDB=2z1p \| SCENE= }}		{{STRUCTURE_2z1p\| PDB=2z1p \| SCENE= }}

	===The Enterococcus faecalis MSCRAMM ACE binds its ligands by the collagen Hug Model===		===The Enterococcus faecalis MSCRAMM ACE binds its ligands by the collagen Hug Model===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17392280}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17392280 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17392280}}		{{ABSTRACT_PUBMED_17392280}}

	==About this Structure==		==About this Structure==
-	~~2Z1P~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA].	+	[[2z1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17392280~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017392280</ref><references group="xtra"/>
	[[Category: Enterococcus faecalis]]		[[Category: Enterococcus faecalis]]
	[[Category: Ponnuraj, K.]]		[[Category: Ponnuraj, K.]]
Line 30:		Line 18:
	[[Category: Collagen]]		[[Category: Collagen]]
	[[Category: Enterococcus faecali]]		[[Category: Enterococcus faecali]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:11:54 2009''

OCA at 11:11, 16 February 2009

2009-02-16T11:11:55Z

←Older revision		Revision as of 11:11, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2Z1P is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA].	+	2Z1P is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA].

	==Reference==		==Reference==
-	The Enterococcus faecalis MSCRAMM ACE binds its ligand by the Collagen Hug model., Liu Q, Ponnuraj K, Xu Y, Ganesh VK, Sillanpaa J, Murray BE, Narayana SV, Hook M, J Biol Chem. 2007 Jul 6;282(27):19629-37. Epub 2007 Mar 28. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17392280 17392280]~~	+	<ref group="xtra">PMID:17392280</ref><references group="xtra"/>
	[[Category: Enterococcus faecalis]]		[[Category: Enterococcus faecalis]]
-	[[Category: Single protein]]
	[[Category: Ponnuraj, K.]]		[[Category: Ponnuraj, K.]]
	[[Category: Ace]]		[[Category: Ace]]
Line 32:		Line 31:
	[[Category: Enterococcus faecali]]		[[Category: Enterococcus faecali]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 15~~:34:~~44 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:11:54 2009''

OCA at 12:34, 27 July 2008

2008-07-27T12:34:48Z

←Older revision		Revision as of 12:34, 27 July 2008
Line 1:		Line 1:
		+	{{Seed}}
	[[Image:2z1p.png\|left\|200px]]		[[Image:2z1p.png\|left\|200px]]

Line 9:		Line 10:
	{{STRUCTURE_2z1p\| PDB=2z1p \| SCENE= }}		{{STRUCTURE_2z1p\| PDB=2z1p \| SCENE= }}

-	~~'''~~The Enterococcus faecalis MSCRAMM ACE binds its ligands by the collagen Hug Model~~'''~~	+	===The Enterococcus faecalis MSCRAMM ACE binds its ligands by the collagen Hug Model===


-	{{~~ABSTRACT_17392280~~}}	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_17392280}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17392280 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17392280}}

	==About this Structure==		==About this Structure==
	2Z1P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA].		2Z1P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1P OCA].
		+
		+	==Reference==
		+	The Enterococcus faecalis MSCRAMM ACE binds its ligand by the Collagen Hug model., Liu Q, Ponnuraj K, Xu Y, Ganesh VK, Sillanpaa J, Murray BE, Narayana SV, Hook M, J Biol Chem. 2007 Jul 6;282(27):19629-37. Epub 2007 Mar 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17392280 17392280]
	[[Category: Enterococcus faecalis]]		[[Category: Enterococcus faecalis]]
	[[Category: Single protein]]		[[Category: Single protein]]
Line 24:		Line 32:
	[[Category: Enterococcus faecali]]		[[Category: Enterococcus faecali]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jun 25 18~~:41:59 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:34:44 2008''