2zcf - Revision history

OCA at 13:31, 1 November 2023

2023-11-01T13:31:46Z

←Older revision		Revision as of 13:31, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='2zcf' size='340' side='right'caption='[[2zcf]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='2zcf' size='340' side='right'caption='[[2zcf]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zcf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium~~ erythropolis~~" gray and thornton 1928~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZCF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zcf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZCF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.43Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ahj\|1ahj]]~~, ~~[[2ahj\|2ahj]], [[2cyz\|2cyz]], [[2cz0\|2cz0]], [[2cz1\|2cz1]], [[2d0q\|2d0q]]~~<~~/div></td></tr>~~	+
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">nthA~~ (~~[https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928]~~)~~, nthB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [https://pdbe.org/2zcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB], [https://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zcf ProSAT], [https://www.topsan.org/Proteins/RSGI/2zcf TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [https://pdbe.org/2zcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB], [https://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zcf ProSAT], [https://www.topsan.org/Proteins/RSGI/2zcf TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NHAA_RHOER NHAA_RHOER]] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class~~. [[https://www.uniprot.org/uniprot/NHAB_RHOER NHAB_RHOER]] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides~~.	+	[https://www.uniprot.org/uniprot/NHAA_RHOER NHAA_RHOER] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 39:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Mycobacterium erythropolis gray and thornton 1928]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Nitrile hydratase~~]]	+	[[Category: Rhodococcus erythropolis]]
-	[[Category: Dohmae, N]]	+	[[Category: Dohmae N]]
-	[[Category: Hashimoto, K]]	+	[[Category: Hashimoto K]]
-	[[Category: Kamiya, N]]	+	[[Category: Kamiya N]]
-	[[Category: Kawano, Y]]	+	[[Category: Kawano Y]]
-	[[Category: Maeda, M]]	+	[[Category: Maeda M]]
-	[[Category: Nakayama, H]]	+	[[Category: Nakayama H]]
-	[[Category: Odaka, M]]	+	[[Category: Odaka M]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Takarada H]]
-	[[Category: Takarada, H]]	+	[[Category: Ueda S]]
-	[[Category: Ueda, S]]	+	[[Category: Yohda M]]
-	[[Category: Yohda, M]]	+
-	~~[[Category: Cysteine-sulfenic acid]]~~	+
-	~~[[Category: Cysteine-sulfinic acid]]~~	+
-	~~[[Category: Hydration]]~~	+
-	~~[[Category: Iron]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: National project on protein structural and functional analyse]]~~	+
-	~~[[Category: Nitrile]]~~	+
-	~~[[Category: Nppsfa]]~~	+
-	~~[[Category: Oxidation]]~~	+
-	~~[[Category: Photo-activation]]~~	+
-	~~[[Category: Photo-reactive]]~~	+
-	~~[[Category: Rsgi~~]]	+

OCA at 07:14, 10 November 2021

2021-11-10T07:14:36Z

OCA at 07:14, 18 October 2018

2018-10-18T07:14:45Z

←Older revision		Revision as of 07:14, 18 October 2018
Line 17:		Line 17:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zc/2zcf_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zc/2zcf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:10, 5 August 2016

2016-08-05T11:10:49Z

←Older revision		Revision as of 11:10, 5 August 2016
Line 1:		Line 1:
		+
	==Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771==		==Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771==
	<StructureSection load='2zcf' size='340' side='right' caption='[[2zcf]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='2zcf' size='340' side='right' caption='[[2zcf]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rhodococcus_erythropolis Rhodococcus~~ erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZCF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium erythropolis" gray and thornton 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZCF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ahj\|1ahj]], [[2ahj\|2ahj]], [[2cyz\|2cyz]], [[2cz0\|2cz0]], [[2cz1\|2cz1]], [[2d0q\|2d0q]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ahj\|1ahj]], [[2ahj\|2ahj]], [[2cyz\|2cyz]], [[2cz0\|2cz0]], [[2cz1\|2cz1]], [[2d0q\|2d0q]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nthA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 ~~Rhodococcus~~ erythropolis]), nthB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 ~~Rhodococcus~~ erythropolis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nthA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928]), nthB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB], [http://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [http://www.topsan.org/Proteins/RSGI/2zcf TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [http://pdbe.org/2zcf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB], [http://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zcf ProSAT], [http://www.topsan.org/Proteins/RSGI/2zcf TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zcf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 30:		Line 31:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2zcf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nitrile hydratase\|Nitrile hydratase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Mycobacterium erythropolis gray and thornton 1928]]
	[[Category: Nitrile hydratase]]		[[Category: Nitrile hydratase]]
-	[[Category: Rhodococcus erythropolis]]
	[[Category: Dohmae, N]]		[[Category: Dohmae, N]]
	[[Category: Hashimoto, K]]		[[Category: Hashimoto, K]]

OCA at 18:19, 25 December 2014

2014-12-25T18:19:24Z

←Older revision		Revision as of 18:19, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2zcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZCF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2zcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZCF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ahj\|1ahj]], [[2ahj\|2ahj]], [[2cyz\|2cyz]], [[2cz0\|2cz0]], [[2cz1\|2cz1]], [[2d0q\|2d0q]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ahj\|1ahj]], [[2ahj\|2ahj]], [[2cyz\|2cyz]], [[2cz0\|2cz0]], [[2cz1\|2cz1]], [[2d0q\|2d0q]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nthA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis]), nthB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nthA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis]), nthB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB], [http://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [http://www.topsan.org/Proteins/RSGI/2zcf TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB], [http://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [http://www.topsan.org/Proteins/RSGI/2zcf TOPSAN]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NHAA_RHOER NHAA_RHOER]] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class. [[http://www.uniprot.org/uniprot/NHAB_RHOER NHAB_RHOER]] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Nitrile hydratase]]		[[Category: Nitrile hydratase]]
	[[Category: Rhodococcus erythropolis]]		[[Category: Rhodococcus erythropolis]]
-	[[Category: Dohmae, N.]]	+	[[Category: Dohmae, N]]
-	[[Category: Hashimoto, K.]]	+	[[Category: Hashimoto, K]]
-	[[Category: Kamiya, N.]]	+	[[Category: Kamiya, N]]
-	[[Category: Kawano, Y.]]	+	[[Category: Kawano, Y]]
-	[[Category: Maeda, M.]]	+	[[Category: Maeda, M]]
-	[[Category: Nakayama, H.]]	+	[[Category: Nakayama, H]]
-	[[Category: Odaka, M.]]	+	[[Category: Odaka, M]]
-	[[Category: ~~RSGI, RIKEN~~ Structural ~~Genomics/Proteomics Initiative.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Takarada, H.]]	+	[[Category: Takarada, H]]
-	[[Category: Ueda, S.]]	+	[[Category: Ueda, S]]
-	[[Category: Yohda, M.]]	+	[[Category: Yohda, M]]
	[[Category: Cysteine-sulfenic acid]]		[[Category: Cysteine-sulfenic acid]]
	[[Category: Cysteine-sulfinic acid]]		[[Category: Cysteine-sulfinic acid]]
Line 57:		Line 59:
	[[Category: Photo-activation]]		[[Category: Photo-activation]]
	[[Category: Photo-reactive]]		[[Category: Photo-reactive]]
-	[[Category: Riken structural genomics/proteomics initiative]]
	[[Category: Rsgi]]		[[Category: Rsgi]]
-	[[Category: Structural genomic]]

OCA at 04:58, 2 October 2014

2014-10-02T04:58:30Z

←Older revision		Revision as of 04:58, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2zcf.~~png~~\|~~left~~\|200px]]	+	==Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771==
		+	<StructureSection load='2zcf' size='340' side='right' caption='[[2zcf]], [[Resolution\|resolution]] 1.43Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2zcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZCF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ahj\|1ahj]], [[2ahj\|2ahj]], [[2cyz\|2cyz]], [[2cz0\|2cz0]], [[2cz1\|2cz1]], [[2d0q\|2d0q]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nthA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis]), nthB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB], [http://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [http://www.topsan.org/Proteins/RSGI/2zcf TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zc/2zcf_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nitrile hydratase (NHase) from Rhodococcus sp. N771 is a non-heme iron enzyme having post-translationally modified cysteine ligands, alphaCys112-SO2H and alphaCys114-SOH. We replaced alphaGln90, which is conserved in all known NHases and involved in the hydrogen-bond network around the catalytic center, with glutamic acid or asparagine. The kcat of alphaQ90E and alphaQ90N mutants decreased to 24% and 5% that of wild type respectively, but the effect of mutations on Km was not very significant. In both mutants, the alphaCys114-SOH modification appeared to be responsible for the catalysis as in native NHase. We crystallized the nitrosylated alphaQ90N mutant and determined its structure at a resolution of 1.43 A. The structure was basically identical to that of native nitrosylated NHase except for the mutated site and its vicinity. The structural difference between native and alphaQ90N mutant NHases suggested the importance of the hydrogen bond networks between alphaGln90 and the iron center for the catalytic activity.

-	~~{{STRUCTURE_2zcf\| PDB=2zcf \| SCENE= }}~~	+	Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771.,Takarada H, Kawano Y, Hashimoto K, Nakayama H, Ueda S, Yohda M, Kamiya N, Dohmae N, Maeda M, Odaka M Biosci Biotechnol Biochem. 2006 Apr;70(4):881-9. PMID:16636455<ref>PMID:16636455</ref>

-	~~===Mutational study on Alpha-Gln90~~ of ~~Fe-type nitrile hydratase from Rhodococcus sp~~. ~~N771===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16636455}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2zcf]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016636455<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Nitrile hydratase]]		[[Category: Nitrile hydratase]]
	[[Category: Rhodococcus erythropolis]]		[[Category: Rhodococcus erythropolis]]

OCA: Protected "2zcf" [edit=sysop:move=sysop]

2013-01-07T22:13:10Z

Protected "2zcf" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:13, 7 January 2013

OCA at 22:13, 7 January 2013

2013-01-07T22:13:00Z

←Older revision		Revision as of 22:13, 7 January 2013
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-	{{Seed}}
	[[Image:2zcf.png\|left\|200px]]		[[Image:2zcf.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2zcf\| PDB=2zcf \| SCENE= }}		{{STRUCTURE_2zcf\| PDB=2zcf \| SCENE= }}

	===Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771===		===Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16636455}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_16636455}}		{{ABSTRACT_PUBMED_16636455}}

	==About this Structure==		==About this Structure==
-	~~2ZCF~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA].	+	[[2zcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16636455~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016636455</ref><references group="xtra"/>
	[[Category: Nitrile hydratase]]		[[Category: Nitrile hydratase]]
	[[Category: Rhodococcus erythropolis]]		[[Category: Rhodococcus erythropolis]]
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	[[Category: Rsgi]]		[[Category: Rsgi]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:58:05 2009''

OCA at 23:58, 17 February 2009

2009-02-17T23:58:06Z

←Older revision		Revision as of 23:58, 17 February 2009
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	==Reference==		==Reference==
-	Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771., Takarada H, Kawano Y, Hashimoto K, Nakayama H, Ueda S, Yohda M, Kamiya N, Dohmae N, Maeda M, Odaka M, Biosci Biotechnol Biochem. 2006 Apr;70(4):881-9. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16636455 16636455]~~	+	<ref group="xtra">PMID:16636455</ref><references group="xtra"/>
	[[Category: Nitrile hydratase]]		[[Category: Nitrile hydratase]]
	[[Category: Rhodococcus erythropolis]]		[[Category: Rhodococcus erythropolis]]
Line 53:		Line 53:
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Nov 16 17~~:20:~~25 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:58:05 2009''

OCA at 15:20, 16 November 2008

2008-11-16T15:20:38Z

←Older revision		Revision as of 15:20, 16 November 2008
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	==About this Structure==		==About this Structure==
-	2ZCF is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA].	+	2ZCF is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA].

	==Reference==		==Reference==
	Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771., Takarada H, Kawano Y, Hashimoto K, Nakayama H, Ueda S, Yohda M, Kamiya N, Dohmae N, Maeda M, Odaka M, Biosci Biotechnol Biochem. 2006 Apr;70(4):881-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16636455 16636455]		Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771., Takarada H, Kawano Y, Hashimoto K, Nakayama H, Ueda S, Yohda M, Kamiya N, Dohmae N, Maeda M, Odaka M, Biosci Biotechnol Biochem. 2006 Apr;70(4):881-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16636455 16636455]
	[[Category: Nitrile hydratase]]		[[Category: Nitrile hydratase]]
-	[[Category: Protein complex]]
	[[Category: Rhodococcus erythropolis]]		[[Category: Rhodococcus erythropolis]]
	[[Category: Dohmae, N.]]		[[Category: Dohmae, N.]]
Line 54:		Line 53:
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 08~~:50:10 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Nov 16 17:20:25 2008''

←Older revision		Revision as of 07:14, 10 November 2021
Line 1:		Line 1:

	==Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771==		==Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771==
-	<StructureSection load='2zcf' size='340' side='right' caption='[[2zcf]], [[Resolution\|resolution]] 1.43Å' scene=''>	+	<StructureSection load='2zcf' size='340' side='right'caption='[[2zcf]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zcf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium erythropolis" gray and thornton 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ZCF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zcf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium erythropolis" gray and thornton 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZCF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ahj\|1ahj]], [[2ahj\|2ahj]], [[2cyz\|2cyz]], [[2cz0\|2cz0]], [[2cz1\|2cz1]], [[2d0q\|2d0q]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ahj\|1ahj]], [[2ahj\|2ahj]], [[2cyz\|2cyz]], [[2cz0\|2cz0]], [[2cz1\|2cz1]], [[2d0q\|2d0q]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nthA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928]), nthB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nthA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928]), nthB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [~~http~~://pdbe.org/2zcf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2zcf ProSAT], [~~http~~://www.topsan.org/Proteins/RSGI/2zcf TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [https://pdbe.org/2zcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB], [https://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zcf ProSAT], [https://www.topsan.org/Proteins/RSGI/2zcf TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NHAA_RHOER NHAA_RHOER]] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class. [[~~http~~://www.uniprot.org/uniprot/NHAB_RHOER NHAB_RHOER]] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides.	+	[[https://www.uniprot.org/uniprot/NHAA_RHOER NHAA_RHOER]] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class. [[https://www.uniprot.org/uniprot/NHAB_RHOER NHAB_RHOER]] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 40:		Line 40:
	</StructureSection>		</StructureSection>
	[[Category: Mycobacterium erythropolis gray and thornton 1928]]		[[Category: Mycobacterium erythropolis gray and thornton 1928]]
		+	[[Category: Large Structures]]
	[[Category: Nitrile hydratase]]		[[Category: Nitrile hydratase]]
	[[Category: Dohmae, N]]		[[Category: Dohmae, N]]