2zfd - Revision history

OCA at 13:57, 13 March 2024

2024-03-13T13:57:17Z

←Older revision		Revision as of 13:57, 13 March 2024
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	<StructureSection load='2zfd' size='340' side='right'caption='[[2zfd]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='2zfd' size='340' side='right'caption='[[2zfd]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zfd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZFD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zfd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZFD FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>~~, ~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1uhn\|1uhn]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><td class="sblockDat">CBL2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), CIPK14 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [https://pdbe.org/2zfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [https://www.ebi.ac.uk/pdbsum/2zfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zfd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [https://pdbe.org/2zfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [https://www.ebi.ac.uk/pdbsum/2zfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zfd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CNBL2_ARATH CNBL2_ARATH]] Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Binds four calcium ions per subunit. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Mediates the inactivation of the proton pump AHA2 by CIPK11. Probably involved in regulating signaling responses to abscisic acid.<ref>PMID:17483306</ref> <ref>PMID:17898163</ref> <ref>PMID:22547024</ref> [[https://www.uniprot.org/uniprot/CIPKE_ARATH CIPKE_ARATH]] CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity).	+	[https://www.uniprot.org/uniprot/CNBL2_ARATH CNBL2_ARATH] Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Binds four calcium ions per subunit. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Mediates the inactivation of the proton pump AHA2 by CIPK11. Probably involved in regulating signaling responses to abscisic acid.<ref>PMID:17483306</ref> <ref>PMID:17898163</ref> <ref>PMID:22547024</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zfd ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zfd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Calcium signals mediate a multitude of plant responses to external stimuli. Calcineurin B-like (CBL) proteins and their target kinases, CBL-interacting protein kinases (CIPKs), represent important relays in plant calcium signaling. CBL interacts with CIPK through a conserved motif (NAF/FISL motif) within the C-terminal regulatory domain. To better understand the functional role of the CBL-CIPK system, we determined the crystal structure of AtCBL2 in complex with the regulatory domain of AtCIPK14 at 1.2 A resolution. The NAF/FISL motif is inserted into a hydrophobic crevice within AtCBL2, accompanied by a large displacement of the helices and loop on the opposite side of the NAF/FISL motif from the C-terminal region, which shields the hydrophobic crevice in free form. Ca(2+) are coordinated within four EF hands in AtCBL2 in bound form. This calcium coordination pattern differs from that in the structure of the SOS3-SOS2 complex previously reported. Structural comparison of the two structures shows that the recognition of CBL by CIPK is performed in a similar manner, but inherent interactions confer binding affinity and specificity.
-
-	The crystal structure of plant-specific calcium-binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14.,Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, Shimizu T J Mol Biol. 2008 Mar 14;377(1):246-57. Epub 2008 Jan 11. PMID:18237745<ref>PMID:18237745</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2zfd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Akaboshi, M]]	+	[[Category: Akaboshi M]]
-	[[Category: Hashimoto, H]]	+	[[Category: Hashimoto H]]
-	[[Category: Ishida, H]]	+	[[Category: Ishida H]]
-	[[Category: Koizumi, N]]	+	[[Category: Koizumi N]]
-	[[Category: Sato, M]]	+	[[Category: Sato M]]
-	[[Category: Shimizu, T]]	+	[[Category: Shimizu T]]
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Calcium binding protein]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Serine/threonine-protein kinase]]~~	+
-	~~[[Category: Signaling protein-transferase complex]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:51, 8 December 2021

2021-12-08T10:51:45Z

←Older revision		Revision as of 10:51, 8 December 2021
Line 1:		Line 1:

	==The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14==		==The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14==
-	<StructureSection load='2zfd' size='340' side='right' caption='[[2zfd]], [[Resolution\|resolution]] 1.20Å' scene=''>	+	<StructureSection load='2zfd' size='340' side='right'caption='[[2zfd]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zfd]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ZFD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zfd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZFD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uhn\|1uhn]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1uhn\|1uhn]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBL2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), CIPK14 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBL2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), CIPK14 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [~~http~~://pdbe.org/2zfd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2zfd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2zfd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [https://pdbe.org/2zfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [https://www.ebi.ac.uk/pdbsum/2zfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zfd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CNBL2_ARATH CNBL2_ARATH]] Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Binds four calcium ions per subunit. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Mediates the inactivation of the proton pump AHA2 by CIPK11. Probably involved in regulating signaling responses to abscisic acid.<ref>PMID:17483306</ref> <ref>PMID:17898163</ref> <ref>PMID:22547024</ref> [[~~http~~://www.uniprot.org/uniprot/CIPKE_ARATH CIPKE_ARATH]] CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity).	+	[[https://www.uniprot.org/uniprot/CNBL2_ARATH CNBL2_ARATH]] Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Binds four calcium ions per subunit. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Mediates the inactivation of the proton pump AHA2 by CIPK11. Probably involved in regulating signaling responses to abscisic acid.<ref>PMID:17483306</ref> <ref>PMID:17898163</ref> <ref>PMID:22547024</ref> [[https://www.uniprot.org/uniprot/CIPKE_ARATH CIPKE_ARATH]] CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Arath]]		[[Category: Arath]]
		+	[[Category: Large Structures]]
	[[Category: Akaboshi, M]]		[[Category: Akaboshi, M]]
	[[Category: Hashimoto, H]]		[[Category: Hashimoto, H]]

OCA at 06:57, 18 October 2018

2018-10-18T06:57:46Z

←Older revision		Revision as of 06:57, 18 October 2018
Line 1:		Line 1:
		+
	==The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14==		==The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14==
	<StructureSection load='2zfd' size='340' side='right' caption='[[2zfd]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='2zfd' size='340' side='right' caption='[[2zfd]], [[Resolution\|resolution]] 1.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uhn\|1uhn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uhn\|1uhn]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), CIPK14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), CIPK14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [http://pdbe.org/2zfd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [http://www.ebi.ac.uk/pdbsum/2zfd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [http://pdbe.org/2zfd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [http://www.ebi.ac.uk/pdbsum/2zfd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zfd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zf/2zfd_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zf/2zfd_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 18:03, 8 February 2016

2016-02-08T18:03:51Z

←Older revision		Revision as of 18:03, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='2zfd' size='340' side='right' caption='[[2zfd]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='2zfd' size='340' side='right' caption='[[2zfd]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zfd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZFD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zfd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZFD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uhn\|1uhn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uhn\|1uhn]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~]), CIPK14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), CIPK14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [http://www.ebi.ac.uk/pdbsum/2zfd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [http://pdbe.org/2zfd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [http://www.ebi.ac.uk/pdbsum/2zfd PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/CNBL2_ARATH CNBL2_ARATH]] Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Binds four calcium ions per subunit. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Mediates the inactivation of the proton pump AHA2 by CIPK11. Probably involved in regulating signaling responses to abscisic acid.<ref>PMID:17483306</ref> <ref>PMID:17898163</ref> <ref>PMID:22547024</ref>	+	[[http://www.uniprot.org/uniprot/CNBL2_ARATH CNBL2_ARATH]] Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Binds four calcium ions per subunit. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Mediates the inactivation of the proton pump AHA2 by CIPK11. Probably involved in regulating signaling responses to abscisic acid.<ref>PMID:17483306</ref> <ref>PMID:17898163</ref> <ref>PMID:22547024</ref> [[http://www.uniprot.org/uniprot/CIPKE_ARATH CIPKE_ARATH]] CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zfd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2zfd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabidopsis thaliana~~]]	+	[[Category: Arath]]
	[[Category: Akaboshi, M]]		[[Category: Akaboshi, M]]
	[[Category: Hashimoto, H]]		[[Category: Hashimoto, H]]

OCA at 01:11, 25 December 2014

2014-12-25T01:11:30Z

←Older revision		Revision as of 01:11, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [http://www.ebi.ac.uk/pdbsum/2zfd PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [http://www.ebi.ac.uk/pdbsum/2zfd PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CNBL2_ARATH CNBL2_ARATH]] Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Binds four calcium ions per subunit. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Mediates the inactivation of the proton pump AHA2 by CIPK11. Probably involved in regulating signaling responses to abscisic acid.<ref>PMID:17483306</ref> <ref>PMID:17898163</ref> <ref>PMID:22547024</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
-	[[Category: Akaboshi, M.]]	+	[[Category: Akaboshi, M]]
-	[[Category: Hashimoto, H.]]	+	[[Category: Hashimoto, H]]
-	[[Category: Ishida, H.]]	+	[[Category: Ishida, H]]
-	[[Category: Koizumi, N.]]	+	[[Category: Koizumi, N]]
-	[[Category: Sato, M.]]	+	[[Category: Sato, M]]
-	[[Category: Shimizu, T.]]	+	[[Category: Shimizu, T]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Calcium binding protein]]		[[Category: Calcium binding protein]]

OCA at 06:27, 10 October 2014

2014-10-10T06:27:51Z

←Older revision		Revision as of 06:27, 10 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2zfd.~~png~~\|~~left~~\|200px]]	+	==The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14==
		+	<StructureSection load='2zfd' size='340' side='right' caption='[[2zfd]], [[Resolution\|resolution]] 1.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2zfd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZFD FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uhn\|1uhn]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), CIPK14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [http://www.ebi.ac.uk/pdbsum/2zfd PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zf/2zfd_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Calcium signals mediate a multitude of plant responses to external stimuli. Calcineurin B-like (CBL) proteins and their target kinases, CBL-interacting protein kinases (CIPKs), represent important relays in plant calcium signaling. CBL interacts with CIPK through a conserved motif (NAF/FISL motif) within the C-terminal regulatory domain. To better understand the functional role of the CBL-CIPK system, we determined the crystal structure of AtCBL2 in complex with the regulatory domain of AtCIPK14 at 1.2 A resolution. The NAF/FISL motif is inserted into a hydrophobic crevice within AtCBL2, accompanied by a large displacement of the helices and loop on the opposite side of the NAF/FISL motif from the C-terminal region, which shields the hydrophobic crevice in free form. Ca(2+) are coordinated within four EF hands in AtCBL2 in bound form. This calcium coordination pattern differs from that in the structure of the SOS3-SOS2 complex previously reported. Structural comparison of the two structures shows that the recognition of CBL by CIPK is performed in a similar manner, but inherent interactions confer binding affinity and specificity.

-	~~{{STRUCTURE_2zfd\| PDB=2zfd \| SCENE= }}~~	+	The crystal structure of plant-specific calcium-binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14.,Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, Shimizu T J Mol Biol. 2008 Mar 14;377(1):246-57. Epub 2008 Jan 11. PMID:18237745<ref>PMID:18237745</ref>

-	~~===The crystal structure~~ of ~~plant specific calcium binding protein AtCBL2 in complex with~~ the ~~regulatory domain of AtCIPK14===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18237745}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2zfd]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018237745<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Akaboshi, M.]]		[[Category: Akaboshi, M.]]

OCA: Protected "2zfd" [edit=sysop:move=sysop]

2013-01-07T21:19:48Z

Protected "2zfd" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:19, 7 January 2013

OCA at 21:19, 7 January 2013

2013-01-07T21:19:45Z

←Older revision		Revision as of 21:19, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2zfd.png\|left\|200px]]		[[Image:2zfd.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2zfd", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2zfd\| PDB=2zfd \| SCENE= }}		{{STRUCTURE_2zfd\| PDB=2zfd \| SCENE= }}

	===The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14===		===The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18237745}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18237745 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18237745}}		{{ABSTRACT_PUBMED_18237745}}

	==About this Structure==		==About this Structure==
-	~~2ZFD~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA].	+	[[2zfd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18237745~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018237745</ref><references group="xtra"/>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Akaboshi, M.]]		[[Category: Akaboshi, M.]]
Line 37:		Line 25:
	[[Category: Protein-protein complex]]		[[Category: Protein-protein complex]]
	[[Category: Serine/threonine-protein kinase]]		[[Category: Serine/threonine-protein kinase]]
-	[[Category: Signaling protein/transferase complex]]	+	[[Category: Signaling protein-transferase complex]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:59:25 2009''

OCA at 22:59, 16 February 2009

2009-02-16T22:59:26Z

←Older revision		Revision as of 22:59, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2ZFD is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA].	+	2ZFD is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA].

	==Reference==		==Reference==
-	The Crystal Structure of Plant-Specific Calcium-Binding Protein AtCBL2 in Complex with the Regulatory Domain of AtCIPK14., Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, Shimizu T, J Mol Biol. 2008 Jan 11;. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18237745 18237745]~~	+	<ref group="xtra">PMID:18237745</ref><references group="xtra"/>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
-	[[Category: Protein complex]]
	[[Category: Akaboshi, M.]]		[[Category: Akaboshi, M.]]
	[[Category: Hashimoto, H.]]		[[Category: Hashimoto, H.]]
Line 41:		Line 40:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 05~~:31:~~51 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:59:25 2009''

OCA at 02:31, 28 July 2008

2008-07-28T02:31:53Z

←Older revision		Revision as of 02:31, 28 July 2008
Line 1:		Line 1:
-	[[Image:2zfd.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2zfd.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2zfd\| PDB=2zfd \| SCENE= }}		{{STRUCTURE_2zfd\| PDB=2zfd \| SCENE= }}

-	~~'''~~The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14~~'''~~	+	===The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14===


-	~~==Overview==~~	+	<!--
-	~~Calcium signals mediate a multitude of plant responses to external stimuli. Calcineurin B~~-~~like (CBL) proteins and their target kinases, CBL~~-~~interacting protein kinases (CIPKs)~~, represent important relays in plant calcium signaling. CBL interacts with CIPK through a conserved motif (NAF/FISL motif) within the C-terminal regulatory domain. To better understand the functional role of the CBL-CIPK system, ~~we determined~~ the ~~crystal structure of AtCBL2 in complex with~~ the ~~regulatory domain of AtCIPK14~~ at 1.~~2 A resolution~~. ~~The NAF/FISL motif~~ is ~~inserted into a hydrophobic crevice within AtCBL2, accompanied by a large displacement of~~ the ~~helices and loop on the opposite side of the NAF/FISL motif from the C-terminal region, which shields the hydrophobic crevice in free form~~. ~~Ca(2+) are coordinated within four EF hands in AtCBL2 in bound form. This calcium coordination pattern differs from that in the structure of the SOS3~~-SOS2 complex previously reported. Structural comparison of the two structures shows that the recognition of CBL by CIPK is performed in a similar manner, but inherent interactions confer binding affinity and specificity.	+	The line below this paragraph, {{ABSTRACT_PUBMED_18237745}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18237745 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18237745}}

	==About this Structure==		==About this Structure==
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	[[Category: Signaling protein/transferase complex]]		[[Category: Signaling protein/transferase complex]]
	[[Category: Transferase]]		[[Category: Transferase]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Apr 24 09~~:32:00 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:31:51 2008''