2zgt - Revision history

OCA at 13:35, 1 November 2023

2023-11-01T13:35:56Z

←Older revision		Revision as of 13:35, 1 November 2023
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	<StructureSection load='2zgt' size='340' side='right'caption='[[2zgt]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2zgt' size='340' side='right'caption='[[2zgt]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zgt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Agrae Agrae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZGT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zgt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyclocybe_aegerita Cyclocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZGT FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</div></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">AAL ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=5400 AGRAE])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [https://pdbe.org/2zgt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [https://www.ebi.ac.uk/pdbsum/2zgt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [https://pdbe.org/2zgt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [https://www.ebi.ac.uk/pdbsum/2zgt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~ATLE_AGRAE ATLE_AGRAE]~~] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells.<ref>PMID:12757412</ref>	+	[https://www.uniprot.org/uniprot/ATLE_CYCAE ATLE_CYCAE] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells. Binds N-acetylneuraminyl lactose (N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucose) (PubMed:16051274).<ref>PMID:12757412</ref> <ref>PMID:16051274</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Agrae~~]]	+	[[Category: Cyclocybe aegerita]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Li~~, D F~~]]	+	[[Category: Li DF]]
-	[[Category: Wang~~, D C~~]]	+	[[Category: Wang DC]]
-	[[Category: Yang, N]]	+	[[Category: Yang N]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Galectin]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Jelly roll]]~~	+
-	~~[[Category: Nuclease~~]]	+

OCA at 07:15, 10 November 2021

2021-11-10T07:15:54Z

←Older revision		Revision as of 07:15, 10 November 2021
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	==Crystal structure of Agrocybe aegerita lectin AAL mutant F93G==		==Crystal structure of Agrocybe aegerita lectin AAL mutant F93G==
-	<StructureSection load='2zgt' size='340' side='right' caption='[[2zgt]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='2zgt' size='340' side='right'caption='[[2zgt]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zgt]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Agrae Agrae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ZGT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zgt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrae Agrae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZGT FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAL ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 AGRAE])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 AGRAE])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [~~http~~://pdbe.org/2zgt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2zgt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [https://pdbe.org/2zgt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [https://www.ebi.ac.uk/pdbsum/2zgt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ATLE_AGRAE ATLE_AGRAE]] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells.<ref>PMID:12757412</ref>	+	[[https://www.uniprot.org/uniprot/ATLE_AGRAE ATLE_AGRAE]] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells.<ref>PMID:12757412</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Agrae]]		[[Category: Agrae]]
		+	[[Category: Large Structures]]
	[[Category: Li, D F]]		[[Category: Li, D F]]
	[[Category: Wang, D C]]		[[Category: Wang, D C]]

OCA at 07:27, 18 October 2018

2018-10-18T07:27:41Z

←Older revision		Revision as of 07:27, 18 October 2018
Line 1:		Line 1:
		+
	==Crystal structure of Agrocybe aegerita lectin AAL mutant F93G==		==Crystal structure of Agrocybe aegerita lectin AAL mutant F93G==
	<StructureSection load='2zgt' size='340' side='right' caption='[[2zgt]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2zgt' size='340' side='right' caption='[[2zgt]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 AGRAE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 AGRAE])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [http://pdbe.org/2zgt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [http://www.ebi.ac.uk/pdbsum/2zgt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [http://pdbe.org/2zgt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [http://www.ebi.ac.uk/pdbsum/2zgt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zg/2zgt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zg/2zgt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:43, 8 February 2016

2016-02-08T20:43:07Z

←Older revision		Revision as of 20:43, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='2zgt' size='340' side='right' caption='[[2zgt]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2zgt' size='340' side='right' caption='[[2zgt]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zgt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Agrocybe_aegerita Agrocybe aegerita~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zgt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrae Agrae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGT FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 ~~Agrocybe aegerita~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 AGRAE])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [http://www.ebi.ac.uk/pdbsum/2zgt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [http://pdbe.org/2zgt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [http://www.ebi.ac.uk/pdbsum/2zgt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zgt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2zgt" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Agrocybe aegerita~~]]	+	[[Category: Agrae]]
	[[Category: Li, D F]]		[[Category: Li, D F]]
	[[Category: Wang, D C]]		[[Category: Wang, D C]]

OCA at 02:01, 25 December 2014

2014-12-25T02:01:29Z

←Older revision		Revision as of 02:01, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2zgt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrocybe_aegerita Agrocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2zgt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrocybe_aegerita Agrocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 Agrocybe aegerita])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 Agrocybe aegerita])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [http://www.ebi.ac.uk/pdbsum/2zgt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [http://www.ebi.ac.uk/pdbsum/2zgt PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ATLE_AGRAE ATLE_AGRAE]] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells.<ref>PMID:12757412</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Agrocybe aegerita]]		[[Category: Agrocybe aegerita]]
-	[[Category: Li, D F.]]	+	[[Category: Li, D F]]
-	[[Category: Wang, D C.]]	+	[[Category: Wang, D C]]
-	[[Category: Yang, N.]]	+	[[Category: Yang, N]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Galectin]]		[[Category: Galectin]]

OCA at 05:08, 2 October 2014

2014-10-02T05:08:49Z

←Older revision		Revision as of 05:08, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2zgt.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Agrocybe aegerita lectin AAL mutant F93G==
		+	<StructureSection load='2zgt' size='340' side='right' caption='[[2zgt]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2zgt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrocybe_aegerita Agrocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zgk\|2zgk]], [[2zgl\|2zgl]], [[2zgm\|2zgm]], [[2zgn\|2zgn]], [[2zgo\|2zgo]], [[2zgp\|2zgp]], [[2zgq\|2zgq]], [[2zgr\|2zgr]], [[2zgs\|2zgs]], [[2zgu\|2zgu]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 Agrocybe aegerita])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zgt RCSB], [http://www.ebi.ac.uk/pdbsum/2zgt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zg/2zgt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lectin AAL (Agrocybe aegerita lectin) from the edible mushroom A. aegerita is an antitumor protein that exerts its tumor-suppressing function via apoptosis-inducing activity in cancer cells. The crystal structures of ligand-free AAL and its complex with lactose have been determined. The AAL structure shows a dimeric organization, and each protomer adopts a prototype galectin fold. To identify the structural determinants for antitumor effects arising from the apoptosis-inducing activity of AAL, 11 mutants were prepared and subjected to comprehensive investigations covering oligomerization detection, carbohydrate binding test, apoptosis-inducing activity assay, and X-ray crystallographic analysis. The results show that dimerization of AAL is a prerequisite for its tumor cell apoptosis-inducing activity, and both galactose and glucose are basic moieties of functional carbohydrate ligands for lectin bioactivity. Furthermore, we have identified a hydrophobic pocket that is essential for the protein's apoptosis-inducing activity but independent of its carbohydrate binding and dimer formation. This hydrophobic pocket comprises a hydrophobic cluster including residues Leu33, Leu35, Phe93, and Ile144, and is involved in AAL's function mechanism as an integrated structural motif. Single mutants such as F93G or I144G do not disrupt carbohydrate binding and homodimerization capabilities, but abolish the bioactivity of the protein. These findings reveal the structural basis for the antitumor property of AAL, which may lead to de novo designs of antitumor drugs based on AAL as a prototype model.

-	~~{{STRUCTURE_2zgt\| PDB=2zgt \| SCENE= }}~~	+	Structural basis for the tumor cell apoptosis-inducing activity of an antitumor lectin from the edible mushroom Agrocybe aegerita.,Yang N, Li DF, Feng L, Xiang Y, Liu W, Sun H, Wang DC J Mol Biol. 2009 Apr 3;387(3):694-705. Epub 2009 Feb 9. PMID:19361423<ref>PMID:19361423</ref>

-	~~===Crystal structure~~ of ~~Agrocybe aegerita lectin AAL mutant F93G===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19361423}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2zgt]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Agrocybe_aegerita Agrocybe aegerita]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019361423<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Agrocybe aegerita]]		[[Category: Agrocybe aegerita]]
	[[Category: Li, D F.]]		[[Category: Li, D F.]]

OCA: Protected "2zgt" [edit=sysop:move=sysop]

2013-01-07T22:30:22Z

Protected "2zgt" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:30, 7 January 2013

OCA at 22:30, 7 January 2013

2013-01-07T22:30:18Z

←Older revision		Revision as of 22:30, 7 January 2013
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-	{{Seed}}
	[[Image:2zgt.png\|left\|200px]]		[[Image:2zgt.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2zgt\| PDB=2zgt \| SCENE= }}		{{STRUCTURE_2zgt\| PDB=2zgt \| SCENE= }}

	===Crystal structure of Agrocybe aegerita lectin AAL mutant F93G===		===Crystal structure of Agrocybe aegerita lectin AAL mutant F93G===

-
-	<!--
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	{{ABSTRACT_PUBMED_19361423}}		{{ABSTRACT_PUBMED_19361423}}

	==About this Structure==		==About this Structure==
-	~~2ZGT~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Agrocybe_aegerita Agrocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA].	+	[[2zgt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrocybe_aegerita Agrocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19361423~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019361423</ref><references group="xtra"/>
	[[Category: Agrocybe aegerita]]		[[Category: Agrocybe aegerita]]
	[[Category: Li, D F.]]		[[Category: Li, D F.]]
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	[[Category: Jelly roll]]		[[Category: Jelly roll]]
	[[Category: Nuclease]]		[[Category: Nuclease]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jan 18 09:13:32 2010''

OCA at 07:13, 18 January 2010

2010-01-18T07:13:33Z

←Older revision		Revision as of 07:13, 18 January 2010
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	===Crystal structure of Agrocybe aegerita lectin AAL mutant F93G===		===Crystal structure of Agrocybe aegerita lectin AAL mutant F93G===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19361423}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19361423 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19361423}}

	==About this Structure==		==About this Structure==
	2ZGT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Agrocybe_aegerita Agrocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA].		2ZGT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Agrocybe_aegerita Agrocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19361423</ref><references group="xtra"/>
	[[Category: Agrocybe aegerita]]		[[Category: Agrocybe aegerita]]
	[[Category: Li, D F.]]		[[Category: Li, D F.]]
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	[[Category: Nuclease]]		[[Category: Nuclease]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jun 10 21~~:59:~~59 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jan 18 09:13:32 2010''

OCA at 18:55, 10 June 2009

2009-06-10T18:55:49Z

←Older revision		Revision as of 18:55, 10 June 2009
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	===Crystal structure of Agrocybe aegerita lectin AAL mutant F93G===		===Crystal structure of Agrocybe aegerita lectin AAL mutant F93G===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19361423}}, adds the Publication Abstract to the page
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-	{{ABSTRACT_PUBMED_19361423}}

	==About this Structure==		==About this Structure==
	2ZGT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Agrocybe_aegerita Agrocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA].		2ZGT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Agrocybe_aegerita Agrocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGT OCA].
-
-	==Reference==
-	<ref group="xtra">PMID:19361423</ref><references group="xtra"/>
	[[Category: Agrocybe aegerita]]		[[Category: Agrocybe aegerita]]
	[[Category: Li, D F.]]		[[Category: Li, D F.]]
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	[[Category: Nuclease]]		[[Category: Nuclease]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 18:09:57 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 21:59:59 2009''