3a4c - Revision history

OCA at 13:59, 13 March 2024

2024-03-13T13:59:36Z

←Older revision		Revision as of 13:59, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='3a4c' size='340' side='right'caption='[[3a4c]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='3a4c' size='340' side='right'caption='[[3a4c]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3a4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3a4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A4C FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2klo\|2klo]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.889Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">Cdt1, Ris2 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [https://pdbe.org/3a4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [https://www.ebi.ac.uk/pdbsum/3a4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [https://pdbe.org/3a4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [https://www.ebi.ac.uk/pdbsum/3a4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CDT1_MOUSE CDT1_MOUSE]] Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.<ref>PMID:11850834</ref> <ref>PMID:12192004</ref> <ref>PMID:14993212</ref>	+	[https://www.uniprot.org/uniprot/CDT1_MOUSE CDT1_MOUSE] Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.<ref>PMID:11850834</ref> <ref>PMID:12192004</ref> <ref>PMID:14993212</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a4c ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a4c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In eukaryotic replication licensing, Cdt1 plays a key role by recruiting the MCM2-7 complex onto the origin of chromosome. The C-terminal domain of mouse Cdt1 (mCdt1C), the most conserved region in Cdt1, is essential for licensing and directly interacts with the MCM2-7 complex. We have determined the structures of mCdt1CS (mCdt1C_small; residues 452 to 557) and mCdt1CL (mCdt1C_large; residues 420 to 557) using X-ray crystallography and solution NMR spectroscopy, respectively. While the N-terminal 31 residues of mCdt1CL form a flexible loop with a short helix near the middle, the rest of mCdt1C folds into a winged helix structure. Together with the middle domain of mouse Cdt1 (mCdt1M, residues 172-368), this study reveals that Cdt1 is formed with a tandem repeat of the winged helix domain. The winged helix fold is also conserved in other licensing factors including archaeal ORC and Cdc6, which supports an idea that these replication initiators may have evolved from a common ancestor. Based on the structure of mCdt1C, in conjunction with the biochemical analysis, we propose a binding site for the MCM complex within the mCdt1C.
-
-	Structure of the Cdt1 C-terminal domain: Conservation of the winged helix fold in replication licensing factors.,Khayrutdinov BI, Bae WJ, Yun YM, Lee JH, Tsuyama T, Kim JJ, Hwang E, Ryu KS, Cheong HK, Cheong C, Ko JS, Enomoto T, Karplus PA, Guntert P, Tada S, Jeon YH, Cho Y Protein Sci. 2009 Aug 31. PMID:19722278<ref>PMID:19722278</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3a4c" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Cho, Y]]	+	[[Category: Cho Y]]
-	[[Category: Lee~~, J H]]~~	+	[[Category: Lee JH]]
-	~~[[Category: Alpha-beta structure]]~~	+
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Dna replication]]~~	+
-	~~[[Category: Dna-binding]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Proto-oncogene]]~~	+
-	~~[[Category: Replication]]~~	+
-	~~[[Category: Ubl conjugation~~]]	+

OCA at 16:16, 22 December 2021

2021-12-22T16:16:35Z

←Older revision		Revision as of 16:16, 22 December 2021
Line 1:		Line 1:

	==Crystal structure of cdt1 C terminal domain==		==Crystal structure of cdt1 C terminal domain==
-	<StructureSection load='3a4c' size='340' side='right' caption='[[3a4c]], [[Resolution\|resolution]] 1.89Å' scene=''>	+	<StructureSection load='3a4c' size='340' side='right'caption='[[3a4c]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3a4c]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3A4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3a4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A4C FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2klo\|2klo]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2klo\|2klo]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cdt1, Ris2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cdt1, Ris2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [~~http~~://pdbe.org/3a4c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3a4c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [https://pdbe.org/3a4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [https://www.ebi.ac.uk/pdbsum/3a4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CDT1_MOUSE CDT1_MOUSE]] Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.<ref>PMID:11850834</ref> <ref>PMID:12192004</ref> <ref>PMID:14993212</ref>	+	[[https://www.uniprot.org/uniprot/CDT1_MOUSE CDT1_MOUSE]] Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.<ref>PMID:11850834</ref> <ref>PMID:12192004</ref> <ref>PMID:14993212</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Cho, Y]]		[[Category: Cho, Y]]

OCA at 07:32, 24 October 2018

2018-10-24T07:32:28Z

←Older revision		Revision as of 07:32, 24 October 2018
Line 1:		Line 1:
		+
	==Crystal structure of cdt1 C terminal domain==		==Crystal structure of cdt1 C terminal domain==
	<StructureSection load='3a4c' size='340' side='right' caption='[[3a4c]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='3a4c' size='340' side='right' caption='[[3a4c]], [[Resolution\|resolution]] 1.89Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2klo\|2klo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2klo\|2klo]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cdt1, Ris2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cdt1, Ris2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [http://pdbe.org/3a4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [http://www.ebi.ac.uk/pdbsum/3a4c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [http://pdbe.org/3a4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [http://www.ebi.ac.uk/pdbsum/3a4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/3a4c_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/3a4c_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 43:		Line 44:
	[[Category: Proto-oncogene]]		[[Category: Proto-oncogene]]
	[[Category: Replication]]		[[Category: Replication]]
		+	[[Category: Ubl conjugation]]

OCA at 19:37, 8 February 2016

2016-02-08T19:37:34Z

←Older revision		Revision as of 19:37, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3a4c' size='340' side='right' caption='[[3a4c]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='3a4c' size='340' side='right' caption='[[3a4c]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3a4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3a4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A4C FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2klo\|2klo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2klo\|2klo]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cdt1, Ris2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cdt1, Ris2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [http://www.ebi.ac.uk/pdbsum/3a4c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [http://pdbe.org/3a4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [http://www.ebi.ac.uk/pdbsum/3a4c PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a4c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3a4c" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Cho, Y]]		[[Category: Cho, Y]]
	[[Category: Lee, J H]]		[[Category: Lee, J H]]

OCA at 01:41, 25 December 2014

2014-12-25T01:41:44Z

←Older revision		Revision as of 01:41, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3a4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A4C FirstGlance]. <br>		<table><tr><td colspan='2'>[[3a4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A4C FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2klo\|2klo]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2klo\|2klo]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cdt1, Ris2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cdt1, Ris2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [http://www.ebi.ac.uk/pdbsum/3a4c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [http://www.ebi.ac.uk/pdbsum/3a4c PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CDT1_MOUSE CDT1_MOUSE]] Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.<ref>PMID:11850834</ref> <ref>PMID:12192004</ref> <ref>PMID:14993212</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Cho, Y.]]	+	[[Category: Cho, Y]]
-	[[Category: Lee, J H.]]	+	[[Category: Lee, J H]]
	[[Category: Alpha-beta structure]]		[[Category: Alpha-beta structure]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]

OCA at 04:49, 2 October 2014

2014-10-02T04:49:31Z

←Older revision		Revision as of 04:49, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~3a4c.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of cdt1 C terminal domain==
		+	<StructureSection load='3a4c' size='340' side='right' caption='[[3a4c]], [[Resolution\|resolution]] 1.89Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3a4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A4C FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2klo\|2klo]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cdt1, Ris2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a4c RCSB], [http://www.ebi.ac.uk/pdbsum/3a4c PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/3a4c_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In eukaryotic replication licensing, Cdt1 plays a key role by recruiting the MCM2-7 complex onto the origin of chromosome. The C-terminal domain of mouse Cdt1 (mCdt1C), the most conserved region in Cdt1, is essential for licensing and directly interacts with the MCM2-7 complex. We have determined the structures of mCdt1CS (mCdt1C_small; residues 452 to 557) and mCdt1CL (mCdt1C_large; residues 420 to 557) using X-ray crystallography and solution NMR spectroscopy, respectively. While the N-terminal 31 residues of mCdt1CL form a flexible loop with a short helix near the middle, the rest of mCdt1C folds into a winged helix structure. Together with the middle domain of mouse Cdt1 (mCdt1M, residues 172-368), this study reveals that Cdt1 is formed with a tandem repeat of the winged helix domain. The winged helix fold is also conserved in other licensing factors including archaeal ORC and Cdc6, which supports an idea that these replication initiators may have evolved from a common ancestor. Based on the structure of mCdt1C, in conjunction with the biochemical analysis, we propose a binding site for the MCM complex within the mCdt1C.

-	~~{{STRUCTURE_3a4c\| PDB=3a4c \| SCENE= }}~~	+	Structure of the Cdt1 C-terminal domain: Conservation of the winged helix fold in replication licensing factors.,Khayrutdinov BI, Bae WJ, Yun YM, Lee JH, Tsuyama T, Kim JJ, Hwang E, Ryu KS, Cheong HK, Cheong C, Ko JS, Enomoto T, Karplus PA, Guntert P, Tada S, Jeon YH, Cho Y Protein Sci. 2009 Aug 31. PMID:19722278<ref>PMID:19722278</ref>

-	~~===Crystal structure~~ of ~~cdt1 C terminal domain===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19722278}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3a4c]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019722278<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Cho, Y.]]		[[Category: Cho, Y.]]

OCA: Protected "3a4c" [edit=sysop:move=sysop]

2013-01-07T21:57:25Z

Protected "3a4c" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:57, 7 January 2013

OCA at 21:57, 7 January 2013

2013-01-07T21:57:21Z

←Older revision		Revision as of 21:57, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3a4c.png\|left\|200px]]		[[Image:3a4c.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3a4c", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3a4c\| PDB=3a4c \| SCENE= }}		{{STRUCTURE_3a4c\| PDB=3a4c \| SCENE= }}

	===Crystal structure of cdt1 C terminal domain===		===Crystal structure of cdt1 C terminal domain===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19722278}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19722278 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19722278}}		{{ABSTRACT_PUBMED_19722278}}

	==About this Structure==		==About this Structure==
-	~~3A4C~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA].	+	[[3a4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19722278~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019722278</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Cho, Y.]]		[[Category: Cho, Y.]]
Line 35:		Line 23:
	[[Category: Proto-oncogene]]		[[Category: Proto-oncogene]]
	[[Category: Replication]]		[[Category: Replication]]
-	[[Category: Ubl conjugation]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 1 14:49:48 2010''

OCA at 12:49, 1 February 2010

2010-02-01T12:49:48Z

←Older revision		Revision as of 12:49, 1 February 2010
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3A4C is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA].	+	3A4C is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4C OCA].

	==Reference==		==Reference==
Line 37:		Line 37:
	[[Category: Ubl conjugation]]		[[Category: Ubl conjugation]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ ~~4 12~~:21:~~56 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 1 14:49:48 2010''

OCA at 10:21, 4 November 2009

2009-11-04T10:21:57Z

←Older revision		Revision as of 10:21, 4 November 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3a4c.~~jpg~~\|left\|200px]]	+	[[Image:3a4c.png\|left\|200px]]

	<!--		<!--
Line 37:		Line 37:
	[[Category: Ubl conjugation]]		[[Category: Ubl conjugation]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Oct 14 10~~:22:58 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 4 12:21:56 2009''