3aaw - Revision history

OCA at 14:17, 1 November 2023

2023-11-01T14:17:09Z

←Older revision		Revision as of 14:17, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3aaw' size='340' side='right'caption='[[3aaw]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3aaw' size='340' side='right'caption='[[3aaw]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3aaw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958~~] and [https://en.wikipedia.org/wiki/~~'corynebacterium_crenatum' 'corynebacterium crenatum'~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3aaw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_crenatum Corynebacterium crenatum] and [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AAW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=LYS:LYSINE'>LYS</scene>~~, ~~<scene name='pdbligand=THR:THREONINE'>THR</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3ab4\|3ab4]], [[3ab2\|3ab2]], [[2dtj\|2dtj]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=THR:THREONINE'>THR</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>lysC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1718~~ "~~Micrococcus glutamicus~~" ~~Kinoshita et al. 1958]), lysC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=168810 'Corynebacterium crenatum'])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaw OCA], [https://pdbe.org/3aaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aaw RCSB], [https://www.ebi.ac.uk/pdbsum/3aaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aaw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaw OCA], [https://pdbe.org/3aaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aaw RCSB], [https://www.ebi.ac.uk/pdbsum/3aaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aaw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AK_CORGL AK_CORGL]] Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids lysine, threonine, isoleucine and methionine.<ref>PMID:17350037</ref> <ref>PMID:20573952</ref>	+	[https://www.uniprot.org/uniprot/AK_CORGL AK_CORGL] Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids lysine, threonine, isoleucine and methionine.<ref>PMID:17350037</ref> <ref>PMID:20573952</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Micrococcus glutamicus kinoshita et al. 1958]]
	[[Category: Corynebacterium crenatum]]		[[Category: Corynebacterium crenatum]]
-	[[Category: ~~Aspartate kinase~~]]	+	[[Category: Corynebacterium glutamicum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kuzuyama, T]]	+	[[Category: Kuzuyama T]]
-	[[Category: Nishiyama, M]]	+	[[Category: Nishiyama M]]
-	[[Category: Tomita, T]]	+	[[Category: Tomita T]]
-	[[Category: Yoshida, A]]	+	[[Category: Yoshida A]]
-	~~[[Category: Alternative initiation]]~~	+
-	~~[[Category: Amino-acid biosynthesis]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Concerted inhibition]]~~	+
-	~~[[Category: Diaminopimelate biosynthesis]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Lysine biosynthesis]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 16:21, 22 December 2021

2021-12-22T16:21:26Z

←Older revision		Revision as of 16:21, 22 December 2021
Line 1:		Line 1:

	==Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine==		==Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine==
-	<StructureSection load='3aaw' size='340' side='right' caption='[[3aaw]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3aaw' size='340' side='right'caption='[[3aaw]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3aaw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958] and [~~http~~://en.wikipedia.org/wiki/'corynebacterium_crenatum' 'corynebacterium crenatum']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3AAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3aaw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958] and [https://en.wikipedia.org/wiki/'corynebacterium_crenatum' 'corynebacterium crenatum']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AAW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=THR:THREONINE'>THR</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=THR:THREONINE'>THR</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ab4\|3ab4]], [[3ab2\|3ab2]], [[2dtj\|2dtj]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ab4\|3ab4]], [[3ab2\|3ab2]], [[2dtj\|2dtj]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lysC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 "Micrococcus glutamicus" Kinoshita et al. 1958]), lysC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=168810 'Corynebacterium crenatum'])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lysC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 "Micrococcus glutamicus" Kinoshita et al. 1958]), lysC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=168810 'Corynebacterium crenatum'])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3aaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaw OCA], [~~http~~://pdbe.org/3aaw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3aaw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3aaw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3aaw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaw OCA], [https://pdbe.org/3aaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aaw RCSB], [https://www.ebi.ac.uk/pdbsum/3aaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aaw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AK_CORGL AK_CORGL]] Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids lysine, threonine, isoleucine and methionine.<ref>PMID:17350037</ref> <ref>PMID:20573952</ref>	+	[[https://www.uniprot.org/uniprot/AK_CORGL AK_CORGL]] Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids lysine, threonine, isoleucine and methionine.<ref>PMID:17350037</ref> <ref>PMID:20573952</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 38:
	[[Category: Corynebacterium crenatum]]		[[Category: Corynebacterium crenatum]]
	[[Category: Aspartate kinase]]		[[Category: Aspartate kinase]]
		+	[[Category: Large Structures]]
	[[Category: Kuzuyama, T]]		[[Category: Kuzuyama, T]]
	[[Category: Nishiyama, M]]		[[Category: Nishiyama, M]]

OCA at 08:07, 24 October 2018

2018-10-24T08:07:22Z

←Older revision		Revision as of 08:07, 24 October 2018
Line 16:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/3aaw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/3aaw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 19:14, 5 August 2016

2016-08-05T19:14:36Z

←Older revision		Revision as of 19:14, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine==		==Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine==
	<StructureSection load='3aaw' size='340' side='right' caption='[[3aaw]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3aaw' size='340' side='right' caption='[[3aaw]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3aaw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Corynebacterium_crenatum Corynebacterium crenatum~~] and [http://en.wikipedia.org/wiki/~~Corynebacterium_glutamicum Corynebacterium glutamicum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3aaw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958] and [http://en.wikipedia.org/wiki/'corynebacterium_crenatum' 'corynebacterium crenatum']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AAW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=THR:THREONINE'>THR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=THR:THREONINE'>THR</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ab4\|3ab4]], [[3ab2\|3ab2]], [[2dtj\|2dtj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ab4\|3ab4]], [[3ab2\|3ab2]], [[2dtj\|2dtj]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 ~~Corynebacterium glutamicum~~]), lysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=168810 Corynebacterium crenatum])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 "Micrococcus glutamicus" Kinoshita et al. 1958]), lysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=168810 'Corynebacterium crenatum'])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aaw RCSB], [http://www.ebi.ac.uk/pdbsum/3aaw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaw OCA], [http://pdbe.org/3aaw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3aaw RCSB], [http://www.ebi.ac.uk/pdbsum/3aaw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3aaw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3aaw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3aaw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspartate kinase~~]]	+	[[Category: Micrococcus glutamicus kinoshita et al. 1958]]
	[[Category: Corynebacterium crenatum]]		[[Category: Corynebacterium crenatum]]
-	[[Category: ~~Corynebacterium glutamicum~~]]	+	[[Category: Aspartate kinase]]
	[[Category: Kuzuyama, T]]		[[Category: Kuzuyama, T]]
	[[Category: Nishiyama, M]]		[[Category: Nishiyama, M]]

OCA at 05:07, 25 December 2014

2014-12-25T05:07:45Z

←Older revision		Revision as of 05:07, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aaw RCSB], [http://www.ebi.ac.uk/pdbsum/3aaw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aaw RCSB], [http://www.ebi.ac.uk/pdbsum/3aaw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AK_CORGL AK_CORGL]] Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids lysine, threonine, isoleucine and methionine.<ref>PMID:17350037</ref> <ref>PMID:20573952</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:10, 12 November 2014

2014-11-12T08:10:33Z

←Older revision		Revision as of 08:10, 12 November 2014
Line 1:		Line 1:
-	[[~~Image:~~3aaw.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine==
		+	<StructureSection load='3aaw' size='340' side='right' caption='[[3aaw]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3aaw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_crenatum Corynebacterium crenatum] and [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AAW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=THR:THREONINE'>THR</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ab4\|3ab4]], [[3ab2\|3ab2]], [[2dtj\|2dtj]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 Corynebacterium glutamicum]), lysC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=168810 Corynebacterium crenatum])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aaw RCSB], [http://www.ebi.ac.uk/pdbsum/3aaw PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/3aaw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aspartate kinase (AK) is the first and committed enzyme of the biosynthetic pathway producing aspartate family amino acids, lysine, threonine, and methionine. AK from Corynebacterium glutamicum (CgAK), a bacterium used for industrial fermentation of amino acids, including glutamate and lysine, is inhibited by lysine and threonine in a concerted manner. To elucidate the mechanism of this unique regulation in CgAK, we determined the crystal structures in several forms: an inhibitory form complexed with both lysine and threonine, an active form complexed with only threonine, and a feedback inhibition-resistant mutant (S301F) complexed with both lysine and threonine. CgAK has a characteristic alpha(2)beta(2)-type heterotetrameric structure made up of two alpha subunits and two beta subunits. Comparison of the crystal structures between inhibitory and active forms revealed that binding inhibitors causes a conformational change to a closed inhibitory form, and the interaction between the catalytic domain in the alpha subunit and beta subunit (regulatory subunit) is a key event for stabilizing the inhibitory form. This study shows not only the first crystal structures of alpha(2)beta(2)-type AK but also the mechanism of concerted inhibition in CgAK.

-	~~{{STRUCTURE_3aaw\| PDB=3aaw \| SCENE= }}~~	+	Mechanism of concerted inhibition of alpha2beta2-type hetero-oligomeric aspartate kinase from Corynebacterium glutamicum.,Yoshida A, Tomita T, Kuzuyama T, Nishiyama M J Biol Chem. 2010 Aug 27;285(35):27477-86. Epub 2010 Jun 23. PMID:20573952<ref>PMID:20573952</ref>

-	~~===Crystal structure~~ of ~~aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20573952}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3aaw]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Corynebacterium_crenatum Corynebacterium crenatum] and [http://en~~.~~wikipedia.org~~/~~wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020573952<~~/~~ref~~><~~ref group="xtra">PMID:017350037</ref><references group="xtra"~~/>	+
	[[Category: Aspartate kinase]]		[[Category: Aspartate kinase]]
	[[Category: Corynebacterium crenatum]]		[[Category: Corynebacterium crenatum]]
	[[Category: Corynebacterium glutamicum]]		[[Category: Corynebacterium glutamicum]]
-	[[Category: Kuzuyama, T.]]	+	[[Category: Kuzuyama, T]]
-	[[Category: Nishiyama, M.]]	+	[[Category: Nishiyama, M]]
-	[[Category: Tomita, T.]]	+	[[Category: Tomita, T]]
-	[[Category: Yoshida, A.]]	+	[[Category: Yoshida, A]]
	[[Category: Alternative initiation]]		[[Category: Alternative initiation]]
	[[Category: Amino-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]
-	[[Category: Aspartate kinase]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Concerted inhibition]]		[[Category: Concerted inhibition]]

OCA at 13:35, 6 February 2013

2013-02-06T13:35:25Z

←Older revision		Revision as of 13:35, 6 February 2013
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	[[Image:3aaw.png\|left\|200px]]		[[Image:3aaw.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3aaw\| PDB=3aaw \| SCENE= }}		{{STRUCTURE_3aaw\| PDB=3aaw \| SCENE= }}

	===Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine===		===Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine===

-
-	<!--
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20573952~~</ref><ref group="xtra">PMID:~~17350037~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020573952</ref><ref group="xtra">PMID:017350037</ref><references group="xtra"/>
	[[Category: Aspartate kinase]]		[[Category: Aspartate kinase]]
	[[Category: Corynebacterium crenatum]]		[[Category: Corynebacterium crenatum]]

OCA: Protected "3aaw" [edit=sysop:move=sysop]

2011-03-14T19:09:48Z

Protected "3aaw" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:09, 14 March 2011

OCA at 19:09, 14 March 2011

2011-03-14T19:09:47Z

←Older revision		Revision as of 19:09, 14 March 2011
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-	{{Seed}}
	[[Image:3aaw.png\|left\|200px]]		[[Image:3aaw.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~3AAW~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Corynebacterium_crenatum Corynebacterium crenatum] and [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA].	+	[[3aaw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_crenatum Corynebacterium crenatum] and [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAW OCA].

	==Reference==		==Reference==
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	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 15:55:26 2010''

OCA at 11:49, 14 July 2010

2010-07-14T11:49:18Z

←Older revision		Revision as of 11:49, 14 July 2010
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	{{Seed}}		{{Seed}}
-	[[Image:3aaw.~~jpg~~\|left\|200px]]	+	[[Image:3aaw.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine===		===Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20573952}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20573952 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20573952}}

	==About this Structure==		==About this Structure==
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:17350037</ref><references group="xtra"/>	+	<ref group="xtra">PMID:20573952</ref><ref group="xtra">PMID:17350037</ref><references group="xtra"/>
	[[Category: Aspartate kinase]]		[[Category: Aspartate kinase]]
	[[Category: Corynebacterium crenatum]]		[[Category: Corynebacterium crenatum]]
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	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jun 23 08~~:48:14 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 15:55:26 2010''