3ao0 - Revision history

OCA at 15:50, 4 October 2023

2023-10-04T15:50:10Z

←Older revision		Revision as of 15:50, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='3ao0' size='340' side='right'caption='[[3ao0]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='3ao0' size='340' side='right'caption='[[3ao0]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ao0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AO0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ao0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AO0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=2A1:(2S)~~-~~2-AMINOPROPAN-1-OL'>2A1</scene>~~, ~~<scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3abo\|3abo]], [[3abq\|3abq]], [[3abr\|3abr]], [[3abs\|3abs]], [[3any\|3any]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2A1:(2S)-2-AMINOPROPAN-1-OL'>2A1</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~eutB, b2441, JW2434 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83333 ECOLI]), eutC, b2440, JW2433~~ (~~[https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]~~)~~</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Ethanolamine_ammonia-lyase Ethanolamine ammonia-lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.7 4.3.1.7]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ao0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ao0 OCA], [https://pdbe.org/3ao0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ao0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ao0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ao0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ao0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ao0 OCA], [https://pdbe.org/3ao0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ao0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ao0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ao0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/EUTB_ECOLI EUTB_ECOLI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ethanolamine ammonia~~-~~lyase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Shibata, N]]	+	[[Category: Shibata N]]
-	~~[[Category: Cobalamin]]~~	+
-	~~[[Category: Cobalt]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 14:58, 29 December 2021

2021-12-29T14:58:40Z

←Older revision		Revision as of 14:58, 29 December 2021
Line 1:		Line 1:

	==Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol==		==Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol==
-	<StructureSection load='3ao0' size='340' side='right' caption='[[3ao0]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='3ao0' size='340' side='right'caption='[[3ao0]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ao0]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3AO0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ao0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AO0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2A1:(2S)-2-AMINOPROPAN-1-OL'>2A1</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2A1:(2S)-2-AMINOPROPAN-1-OL'>2A1</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3abo\|3abo]], [[3abq\|3abq]], [[3abr\|3abr]], [[3abs\|3abs]], [[3any\|3any]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3abo\|3abo]], [[3abq\|3abq]], [[3abr\|3abr]], [[3abs\|3abs]], [[3any\|3any]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eutB, b2441, JW2434 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), eutC, b2440, JW2433 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eutB, b2441, JW2434 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), eutC, b2440, JW2433 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ethanolamine_ammonia-lyase Ethanolamine ammonia-lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.7 4.3.1.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ethanolamine_ammonia-lyase Ethanolamine ammonia-lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.7 4.3.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ao0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ao0 OCA], [~~http~~://pdbe.org/3ao0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ao0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ao0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ao0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ao0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ao0 OCA], [https://pdbe.org/3ao0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ao0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ao0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ao0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	[[Category: Ecoli]]		[[Category: Ecoli]]
	[[Category: Ethanolamine ammonia-lyase]]		[[Category: Ethanolamine ammonia-lyase]]
		+	[[Category: Large Structures]]
	[[Category: Shibata, N]]		[[Category: Shibata, N]]
	[[Category: Cobalamin]]		[[Category: Cobalamin]]

OCA at 09:12, 11 August 2016

2016-08-11T09:12:36Z

←Older revision		Revision as of 09:12, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol==		==Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol==
	<StructureSection load='3ao0' size='340' side='right' caption='[[3ao0]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='3ao0' size='340' side='right' caption='[[3ao0]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ao0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AO0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ao0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AO0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2A1:(2S)-2-AMINOPROPAN-1-OL'>2A1</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2A1:(2S)-2-AMINOPROPAN-1-OL'>2A1</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3abo\|3abo]], [[3abq\|3abq]], [[3abr\|3abr]], [[3abs\|3abs]], [[3any\|3any]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3abo\|3abo]], [[3abq\|3abq]], [[3abr\|3abr]], [[3abs\|3abs]], [[3any\|3any]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eutB, b2441, JW2434 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~]), eutC, b2440, JW2433 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eutB, b2441, JW2434 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), eutC, b2440, JW2433 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ethanolamine_ammonia-lyase Ethanolamine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.7 4.3.1.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ethanolamine_ammonia-lyase Ethanolamine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.7 4.3.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ao0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ao0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ao0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ao0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ao0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ao0 OCA], [http://pdbe.org/3ao0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ao0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ao0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ao0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ao0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Ethanolamine ammonia-lyase]]		[[Category: Ethanolamine ammonia-lyase]]
	[[Category: Shibata, N]]		[[Category: Shibata, N]]

OCA at 10:20, 25 January 2015

2015-01-25T10:20:29Z

←Older revision		Revision as of 10:20, 25 January 2015
Line 23:		Line 23:
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Ethanolamine ammonia-lyase]]		[[Category: Ethanolamine ammonia-lyase]]
-	[[Category: Shibata, N.]]	+	[[Category: Shibata, N]]
	[[Category: Cobalamin]]		[[Category: Cobalamin]]
	[[Category: Cobalt]]		[[Category: Cobalt]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 09:27, 5 November 2014

2014-11-05T09:27:56Z

←Older revision		Revision as of 09:27, 5 November 2014
Line 1:		Line 1:
-	[[~~Image:~~3ao0.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol==
		+	<StructureSection load='3ao0' size='340' side='right' caption='[[3ao0]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ao0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AO0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2A1:(2S)-2-AMINOPROPAN-1-OL'>2A1</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3abo\|3abo]], [[3abq\|3abq]], [[3abr\|3abr]], [[3abs\|3abs]], [[3any\|3any]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eutB, b2441, JW2434 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12]), eutC, b2440, JW2433 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ethanolamine_ammonia-lyase Ethanolamine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.7 4.3.1.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ao0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ao0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ao0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ao0 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Coenzyme B(12)-dependent ethanolamine ammonia-lyase acts on both enantiomers of the substrate 2-amino-1-propanol [Diziol, P., et al. (1980) Eur. J. Biochem. 106, 211-224]. To rationalize this apparent lack of stereospecificity and the enantiomer-specific stereochemical courses of the deamination, we analyzed the X-ray structures of enantiomer-bound forms of the enzyme-cyanocobalamin complex. The lower affinity for the (R)-enantiomer may be due to the conformational change of the Valalpha326 side chain of the enzyme. In a manner consistent with the reported experimental results, we can predict that the pro-S hydrogen atom on C1 is abstracted by the adenosyl radical from both enantiomeric substrates, because it is the nearest one in both enantiomer-bound forms. We also predicted that the NH(2) group migrates from C2 to C1 by a suprafacial shift, with inversion of configuration at C1 for both enantiomeric substrates, although the absolute configuration of the 1-amino-1-propanol intermediate is not yet known. Reported labeling experiments demonstrate that (R)-2-amino-1-propanol is deaminated by the enzyme with inversion of configuration at C2, whereas the (S)-enantiomer is deaminated with retention. By taking these results into consideration, we can predict the rotameric radical intermediate from the (S)-enantiomer undergoes flipping to the rotamer from the (R)-enantiomer before the hydrogen back-abstraction. This suggests the preference of the enzyme active site for the rotamer from the (R)-enantiomer in equilibration. This preference might be explained in terms of the steric repulsion of the (S)-enantiomer-derived product radical at C3 with the Phealpha329 and Leualpha402 residues.

-	~~{{STRUCTURE_3ao0\| PDB=3ao0 \| SCENE= }}~~	+	How coenzyme B12-dependent ethanolamine ammonia-lyase deals with both enantiomers of 2-amino-1-propanol as substrates: structure-based rationalization.,Shibata N, Higuchi Y, Toraya T Biochemistry. 2011 Feb 1;50(4):591-8. Epub 2010 Dec 30. PMID:21142024<ref>PMID:21142024</ref>

-	~~===Crystal structure~~ of ~~ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (~~S~~)-2-amino-1-propanol===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21142024}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3ao0]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021142024<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Ethanolamine ammonia-lyase]]		[[Category: Ethanolamine ammonia-lyase]]

OCA at 06:23, 24 October 2012

2012-10-24T06:23:00Z

←Older revision		Revision as of 06:23, 24 October 2012
Line 1:		Line 1:
-	[[Image:3ao0.~~jpg~~\|left\|200px]]	+	[[Image:3ao0.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ao0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ao0\| PDB=3ao0 \| SCENE= }}		{{STRUCTURE_3ao0\| PDB=3ao0 \| SCENE= }}

	===Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol===		===Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21142024}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21142024 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21142024}}		{{ABSTRACT_PUBMED_21142024}}

	==About this Structure==		==About this Structure==
-	[[3ao0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA].	+	[[3ao0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:021142024</ref><references group="xtra"/>		<ref group="xtra">PMID:021142024</ref><references group="xtra"/>
-	[[Category: Escherichia coli]]	+	[[Category: Escherichia coli k-12]]
	[[Category: Ethanolamine ammonia-lyase]]		[[Category: Ethanolamine ammonia-lyase]]
	[[Category: Shibata, N.]]		[[Category: Shibata, N.]]

OCA: Protected "3ao0" [edit=sysop:move=sysop]

2011-08-03T08:44:48Z

Protected "3ao0" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:44, 3 August 2011

OCA at 08:44, 3 August 2011

2011-08-03T08:44:45Z

←Older revision		Revision as of 08:44, 3 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ao0.jpg\|left\|200px]]

-	The ~~entry 3ao0~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ao0", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ao0\| PDB=3ao0 \| SCENE= }}

-	~~Authors: Shibata, N.~~	+	===Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol===

-	Description: Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Oct 20 06~~:15:~~55 2010''~~	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21142024}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21142024 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21142024}}
		+
		+	==About this Structure==
		+	[[3ao0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AO0 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021142024</ref><references group="xtra"/>
		+	[[Category: Escherichia coli]]
		+	[[Category: Ethanolamine ammonia-lyase]]
		+	[[Category: Shibata, N.]]
		+	[[Category: Cobalamin]]
		+	[[Category: Cobalt]]
		+	[[Category: Lyase]]
		+	[[Category: Tim barrel]]

OCA at 03:05, 20 October 2010

2010-10-20T03:05:50Z

←Older revision		Revision as of 03:05, 20 October 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ao0 is ON HOLD	+	The entry 3ao0 is ON HOLD until Paper Publication

	Authors: Shibata, N.		Authors: Shibata, N.
Line 7:		Line 7:
	Description: Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol		Description: Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct ~~6 05~~:43:04 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 20 06:15:55 2010''

OCA at 02:33, 6 October 2010

2010-10-06T02:33:33Z

←Older revision		Revision as of 02:33, 6 October 2010
Line 5:		Line 5:
	Authors: Shibata, N.		Authors: Shibata, N.

-	Description: ~~CRYSTAL STRUCTURE OF ETHANOLAMINE AMMONIA~~-~~LYASE FROM ESCHERICHIA COLI COMPLEXED WITH~~ CN-CBL ~~AND~~ (S)-2-~~AMINO~~-1-~~PROPANOL~~	+	Description: Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 22 14~~:27:50 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 05:43:04 2010''