3awm - Revision history

OCA at 08:40, 7 February 2024

2024-02-07T08:40:34Z

←Older revision		Revision as of 08:40, 7 February 2024
Line 3:		Line 3:
	<StructureSection load='3awm' size='340' side='right'caption='[[3awm]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3awm' size='340' side='right'caption='[[3awm]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3awm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AWM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3awm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AWM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3awp\|3awp]], [[3awq\|3awq]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fatty-acid_peroxygenase Fatty-acid peroxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.4 1.11.2.4] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3awm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awm OCA], [https://pdbe.org/3awm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3awm RCSB], [https://www.ebi.ac.uk/pdbsum/3awm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3awm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3awm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awm OCA], [https://pdbe.org/3awm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3awm RCSB], [https://www.ebi.ac.uk/pdbsum/3awm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3awm ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/O24782_SPHPI O24782_SPHPI]
-	Cytochrome P450(SPalpha) (CYP152B1) isolated from Sphingomonas paucimobilis is the first P450 to be classified as a H(2)O(2)-dependent P450. P450(SPalpha) hydroxylates fatty acids with high alpha-regioselectivity. Herein we report the crystal structure of P450(SPalpha) with palmitic acid as a substrate at a resolution of 1.65 A. The structure revealed that the C(alpha) of the bound palmitic acid in one of the alternative conformations is 4.5 A from the heme iron. This conformation explains the highly selective alpha-hydroxylation of fatty acid observed in P450(SPalpha). Mutations at the active site and the F-G loop of P450(SPalpha) did not impair its regioselectivity. The crystal structures of mutants (L78F and F288G) revealed that the location of the bound palmitic acid was essentially the same as that in the WT, although amino acids at the active site were replaced with the corresponding amino acids of cytochrome P450(BSbeta) (CYP152A1), which shows beta-regioselectivity. This implies that the high regioselectivity of P450(SPalpha) is caused by the orientation of the hydrophobic channel, which is more perpendicular to the heme plane than that of P450(BSbeta).	+
-		+
-	~~Crystal structure of H2O2-dependent cytochrome P450SPalpha with its bound fatty acid substrate~~: insight into the regioselective hydroxylation of fatty acids at the alpha position.,Fujishiro T, Shoji O, Nagano S, Sugimoto H, Shiro Y, Watanabe Y J Biol Chem. 2011 Aug 26;286(34):29941-50. Epub 2011 Jun 30. PMID:21719702<ref>PMID:21719702</~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3awm" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]		*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus devorans zimmermann 1890]]
-	[[Category: Fatty-acid peroxygenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fujishiro, T]]	+	[[Category: Sphingomonas paucimobilis]]
-	[[Category: Nagano, S]]	+	[[Category: Fujishiro T]]
-	[[Category: Shiro, Y]]	+	[[Category: Nagano S]]
-	[[Category: Shoji, O]]	+	[[Category: Shiro Y]]
-	[[Category: Sugimoto, H]]	+	[[Category: Shoji O]]
-	[[Category: Watanabe, Y]]	+	[[Category: Sugimoto H]]
-	~~[[Category: Cytochrome p450]]~~	+	[[Category: Watanabe Y]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Peroxygenase~~]]	+

OCA at 13:28, 4 May 2022

2022-05-04T13:28:01Z

←Older revision		Revision as of 13:28, 4 May 2022
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	<StructureSection load='3awm' size='340' side='right'caption='[[3awm]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3awm' size='340' side='right'caption='[[3awm]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3awm]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3AWM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3awm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AWM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3awp\|3awp]], [[3awq\|3awq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3awp\|3awp]], [[3awq\|3awq]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Fatty-acid_peroxygenase Fatty-acid peroxygenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.4 1.11.2.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fatty-acid_peroxygenase Fatty-acid peroxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.4 1.11.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3awm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awm OCA], [~~http~~://pdbe.org/3awm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3awm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3awm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3awm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3awm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awm OCA], [https://pdbe.org/3awm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3awm RCSB], [https://www.ebi.ac.uk/pdbsum/3awm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3awm ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:26, 29 July 2020

2020-07-29T12:26:14Z

←Older revision		Revision as of 12:26, 29 July 2020
Line 1:		Line 1:

	==Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid==		==Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid==
-	<StructureSection load='3awm' size='340' side='right' caption='[[3awm]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='3awm' size='340' side='right'caption='[[3awm]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3AWM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3AWM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3awp\|3awp]], [[3awq\|3awq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3awp\|3awp]], [[3awq\|3awq]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fatty-acid_peroxygenase Fatty-acid peroxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.4 1.11.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fatty-acid_peroxygenase Fatty-acid peroxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.4 1.11.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3awm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awm OCA], [http://pdbe.org/3awm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3awm RCSB], [http://www.ebi.ac.uk/pdbsum/3awm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3awm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3awm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awm OCA], [http://pdbe.org/3awm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3awm RCSB], [http://www.ebi.ac.uk/pdbsum/3awm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3awm ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Cytochrome P450\|Cytochrome P450]]	+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	[[Category: Bacillus devorans zimmermann 1890]]		[[Category: Bacillus devorans zimmermann 1890]]
	[[Category: Fatty-acid peroxygenase]]		[[Category: Fatty-acid peroxygenase]]
		+	[[Category: Large Structures]]
	[[Category: Fujishiro, T]]		[[Category: Fujishiro, T]]
	[[Category: Nagano, S]]		[[Category: Nagano, S]]

OCA at 10:41, 4 August 2016

2016-08-04T10:41:53Z

←Older revision		Revision as of 10:41, 4 August 2016
Line 1:		Line 1:
		+
	==Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid==		==Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid==
	<StructureSection load='3awm' size='340' side='right' caption='[[3awm]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3awm' size='340' side='right' caption='[[3awm]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sphingomonas_paucimobilis Sphingomonas paucimobilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AWM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_devorans"_zimmermann_1890 "bacillus devorans" zimmermann 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AWM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3awp\|3awp]], [[3awq\|3awq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3awp\|3awp]], [[3awq\|3awq]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fatty-acid_peroxygenase Fatty-acid peroxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.4 1.11.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fatty-acid_peroxygenase Fatty-acid peroxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.4 1.11.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3awm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3awm RCSB], [http://www.ebi.ac.uk/pdbsum/3awm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3awm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awm OCA], [http://pdbe.org/3awm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3awm RCSB], [http://www.ebi.ac.uk/pdbsum/3awm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3awm ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3awm" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus devorans zimmermann 1890]]
	[[Category: Fatty-acid peroxygenase]]		[[Category: Fatty-acid peroxygenase]]
-	[[Category: Sphingomonas paucimobilis]]
	[[Category: Fujishiro, T]]		[[Category: Fujishiro, T]]
	[[Category: Nagano, S]]		[[Category: Nagano, S]]

OCA at 14:17, 18 December 2014

2014-12-18T14:17:26Z

←Older revision		Revision as of 14:17, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3awm\| PDB=3awm \| SCENE= }}~~	+	==Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid==
-	===Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid===	+	<StructureSection load='3awm' size='340' side='right' caption='[[3awm]], [[Resolution\|resolution]] 1.65Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21719702}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AWM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3awp\|3awp]], [[3awq\|3awq]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fatty-acid_peroxygenase Fatty-acid peroxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.4 1.11.2.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3awm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3awm RCSB], [http://www.ebi.ac.uk/pdbsum/3awm PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytochrome P450(SPalpha) (CYP152B1) isolated from Sphingomonas paucimobilis is the first P450 to be classified as a H(2)O(2)-dependent P450. P450(SPalpha) hydroxylates fatty acids with high alpha-regioselectivity. Herein we report the crystal structure of P450(SPalpha) with palmitic acid as a substrate at a resolution of 1.65 A. The structure revealed that the C(alpha) of the bound palmitic acid in one of the alternative conformations is 4.5 A from the heme iron. This conformation explains the highly selective alpha-hydroxylation of fatty acid observed in P450(SPalpha). Mutations at the active site and the F-G loop of P450(SPalpha) did not impair its regioselectivity. The crystal structures of mutants (L78F and F288G) revealed that the location of the bound palmitic acid was essentially the same as that in the WT, although amino acids at the active site were replaced with the corresponding amino acids of cytochrome P450(BSbeta) (CYP152A1), which shows beta-regioselectivity. This implies that the high regioselectivity of P450(SPalpha) is caused by the orientation of the hydrophobic channel, which is more perpendicular to the heme plane than that of P450(BSbeta).

-	~~==About this Structure==~~	+	Crystal structure of H2O2-dependent cytochrome P450SPalpha with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the alpha position.,Fujishiro T, Shoji O, Nagano S, Sugimoto H, Shiro Y, Watanabe Y J Biol Chem. 2011 Aug 26;286(34):29941-50. Epub 2011 Jun 30. PMID:21719702<ref>PMID:21719702</ref>
-	~~[[3awm]] is a 1 chain~~ structure with ~~sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3AWM OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:021719702</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[Cytochrome P450\|Cytochrome P450]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fatty-acid peroxygenase]]		[[Category: Fatty-acid peroxygenase]]
	[[Category: Sphingomonas paucimobilis]]		[[Category: Sphingomonas paucimobilis]]
-	[[Category: Fujishiro, T.]]	+	[[Category: Fujishiro, T]]
-	[[Category: Nagano, S.]]	+	[[Category: Nagano, S]]
-	[[Category: Shiro, Y.]]	+	[[Category: Shiro, Y]]
-	[[Category: Shoji, O.]]	+	[[Category: Shoji, O]]
-	[[Category: Sugimoto, H.]]	+	[[Category: Sugimoto, H]]
-	[[Category: Watanabe, Y.]]	+	[[Category: Watanabe, Y]]
	[[Category: Cytochrome p450]]		[[Category: Cytochrome p450]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Peroxygenase]]		[[Category: Peroxygenase]]

OCA at 17:58, 19 June 2013

2013-06-19T17:58:00Z

←Older revision		Revision as of 17:58, 19 June 2013
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-	[[Image:3awm.png\|left\|200px]]
-
	{{STRUCTURE_3awm\| PDB=3awm \| SCENE= }}		{{STRUCTURE_3awm\| PDB=3awm \| SCENE= }}
-
	===Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid===		===Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid===
-		+	{{ABSTRACT_PUBMED_21719702}}

	==About this Structure==		==About this Structure==
	[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA].		[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA].

-	==~~See Also~~==	+	==Reference==
-	*[[Cytochrome P450\|Cytochrome P450]]	+	<ref group="xtra">PMID:021719702</ref><references group="xtra"/><references/>
	[[Category: Fatty-acid peroxygenase]]		[[Category: Fatty-acid peroxygenase]]
	[[Category: Sphingomonas paucimobilis]]		[[Category: Sphingomonas paucimobilis]]

OCA at 14:38, 20 October 2012

2012-10-20T14:38:03Z

←Older revision		Revision as of 14:38, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3awm]] is a 1 chain structure ~~of [[Cytochrome P450]]~~ with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA].	+	[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA].

	==See Also==		==See Also==

OCA at 15:45, 26 July 2012

2012-07-26T15:45:41Z

←Older revision		Revision as of 15:45, 26 July 2012
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-	[[Image:3awm.~~jpg~~\|left\|200px]]	+	[[Image:3awm.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3awm", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3awm\| PDB=3awm \| SCENE= }}		{{STRUCTURE_3awm\| PDB=3awm \| SCENE= }}

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	==About this Structure==		==About this Structure==
-	[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA].	+	[[3awm]] is a 1 chain structure of [[Cytochrome P450]] with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA].
		+
		+	==See Also==
		+	*[[Cytochrome P450\|Cytochrome P450]]
	[[Category: Fatty-acid peroxygenase]]		[[Category: Fatty-acid peroxygenase]]
	[[Category: Sphingomonas paucimobilis]]		[[Category: Sphingomonas paucimobilis]]

OCA at 06:15, 29 June 2011

2011-06-29T06:15:03Z

←Older revision		Revision as of 06:15, 29 June 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3awm.jpg\|left\|200px]]

-	The ~~entry 3awm~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3awm", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3awm\| PDB=3awm \| SCENE= }}

-	~~Authors: Fujishiro, T., Shoji, O., Nagano, S., Sugimoto, H., Shiro, Y., Watanabe, Y.~~	+	===Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid===

-	~~Description~~: ~~Cytochrome P450SP alpha (CYP152B1) wild~~-~~type with palmitic~~ acid	+
		+	==About this Structure==
		+	[[3awm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWM OCA].
		+	[[Category: Fatty-acid peroxygenase]]
		+	[[Category: Sphingomonas paucimobilis]]
		+	[[Category: Fujishiro, T.]]
		+	[[Category: Nagano, S.]]
		+	[[Category: Shiro, Y.]]
		+	[[Category: Shoji, O.]]
		+	[[Category: Sugimoto, H.]]
		+	[[Category: Watanabe, Y.]]
		+	[[Category: Cytochrome p450]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Peroxygenase]]

OCA at 04:48, 13 April 2011

2011-04-13T04:48:55Z

←Older revision		Revision as of 04:48, 13 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3awm is ON HOLD	+	The entry 3awm is ON HOLD until Paper Publication

	Authors: Fujishiro, T., Shoji, O., Nagano, S., Sugimoto, H., Shiro, Y., Watanabe, Y.		Authors: Fujishiro, T., Shoji, O., Nagano, S., Sugimoto, H., Shiro, Y., Watanabe, Y.

	Description: Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid		Description: Cytochrome P450SP alpha (CYP152B1) wild-type with palmitic acid