3azu - Revision history

OCA at 06:14, 5 August 2020

2020-08-05T06:14:32Z

←Older revision		Revision as of 06:14, 5 August 2020
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	==X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA==		==X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA==
-	<StructureSection load='3azu' size='340' side='right' caption='[[3azu]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3azu' size='340' side='right'caption='[[3azu]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3AZU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3AZU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://pdbe.org/3azu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3azu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://pdbe.org/3azu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3azu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Azurin\|Azurin]]	+	*[[Azurin 3D structures\|Azurin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Huber, R]]		[[Category: Huber, R]]
	[[Category: Messerschmidt, A]]		[[Category: Messerschmidt, A]]
	[[Category: Nar, H]]		[[Category: Nar, H]]

OCA at 07:44, 31 October 2018

2018-10-31T07:44:42Z

←Older revision		Revision as of 07:44, 31 October 2018
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		+
	==X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA==		==X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA==
	<StructureSection load='3azu' size='340' side='right' caption='[[3azu]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3azu' size='340' side='right' caption='[[3azu]], [[Resolution\|resolution]] 2.10Å' scene=''>
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	<table><tr><td colspan='2'>[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AZU FirstGlance]. <br>		<table><tr><td colspan='2'>[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AZU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://pdbe.org/3azu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://pdbe.org/3azu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3azu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/az/3azu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/az/3azu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:32, 9 February 2016

2016-02-09T23:32:58Z

←Older revision		Revision as of 23:32, 9 February 2016
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AZU FirstGlance]. <br>		<table><tr><td colspan='2'>[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AZU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://pdbe.org/3azu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3azu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3azu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 10:16, 25 December 2014

2014-12-25T10:16:07Z

←Older revision		Revision as of 10:16, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Huber, R.]]	+	[[Category: Huber, R]]
-	[[Category: Messerschmidt, A.]]	+	[[Category: Messerschmidt, A]]
-	[[Category: Nar, H.]]	+	[[Category: Nar, H]]

OCA at 05:57, 10 October 2014

2014-10-10T05:57:23Z

←Older revision		Revision as of 05:57, 10 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AZU FirstGlance]. <br>		<table><tr><td colspan='2'>[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AZU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:06, 16 July 2014

2014-07-16T09:06:11Z

←Older revision		Revision as of 09:06, 16 July 2014
Line 1:		Line 1:
-	[[~~Image~~:3azu.~~png\|left~~\|200px]]	+	==X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA==
		+	<StructureSection load='3azu' size='340' side='right' caption='[[3azu]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AZU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3azu RCSB], [http://www.ebi.ac.uk/pdbsum/3azu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/az/3azu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three-dimensional structures of two site-specific mutants of the blue copper protein azurin from Pseudomonas aeruginosa have been solved by a combination of isomorphous replacement and Patterson search techniques, and refined by energy-restrained least-squares methods. The mutations introduced by recombinant DNA techniques involve residue His35, which was exchanged for glutamine and leucine, to probe for its suggested role in electron transfer. The two mutants, His35Gln (H35Q) and His35Leu (H35L), crystallize non-isomorphously in the orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions of a = 109.74 A, b = 99.15 A, c = 47.82 A for H35Q, a = 57.82 A, b = 81.06 A, c = 110.03 A for H35L. In each crystal form, there are four molecules in the asymmetric unit. They are arranged as a dimer of dimers in the H35Q case and are distorted from ideal C2 symmetry in H35L. The final crystallographic R-value is 16.3% for 20.747 reflections to a resolution of 2.1 A for H35Q and 17.0% for 32,548 reflections to 1.9 A for H35L. The crystal structures reported here represent the first crystallographically refined structures for azurin from P. aeruginosa. The structure is very similar to that of azurin from Alcaligenes denitrificans. The copper atom is located about 7 A below a hydrophobic surface region and is ligated by five donor groups in a distorted trigonal bipyramidal fashion. The implications for electron transfer properties of the protein are discussed in terms of the mutation site and the packing of the molecules within the tetramer.

-	~~{{STRUCTURE_3azu\| PDB=3azu \| SCENE= }}~~	+	X-ray crystal structure of the two site-specific mutants His35Gln and His35Leu of azurin from Pseudomonas aeruginosa.,Nar H, Messerschmidt A, Huber R, van de Kamp M, Canters GW J Mol Biol. 1991 Mar 20;218(2):427-47. PMID:1901363<ref>PMID:1901363</ref>

-	~~===X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_1901363}}~~	+	==See Also==
-		+	*[[Azurin\|Azurin]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3azu~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:001901363<~~/~~ref~~><~~references group="xtra"~~/>	+
-	~~[[Category: Pseudomonas aeruginosa]]~~	+
	[[Category: Huber, R.]]		[[Category: Huber, R.]]
	[[Category: Messerschmidt, A.]]		[[Category: Messerschmidt, A.]]
	[[Category: Nar, H.]]		[[Category: Nar, H.]]

OCA: Protected "3azu" [edit=sysop:move=sysop]

2013-01-07T22:02:04Z

Protected "3azu" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:02, 7 January 2013

OCA at 22:02, 7 January 2013

2013-01-07T22:02:01Z

←Older revision		Revision as of 22:02, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3azu.png\|left\|200px]]		[[Image:3azu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3azu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3azu\| PDB=3azu \| SCENE= }}		{{STRUCTURE_3azu\| PDB=3azu \| SCENE= }}

	===X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA===		===X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_1901363}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 1901363 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_1901363}}		{{ABSTRACT_PUBMED_1901363}}

	==About this Structure==		==About this Structure==
-	~~3AZU~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA].	+	[[3azu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~1901363~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:001901363</ref><references group="xtra"/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Huber, R.]]		[[Category: Huber, R.]]
	[[Category: Messerschmidt, A.]]		[[Category: Messerschmidt, A.]]
	[[Category: Nar, H.]]		[[Category: Nar, H.]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:37:48 2009''

OCA at 11:37, 17 February 2009

2009-02-17T11:37:48Z

←Older revision		Revision as of 11:37, 17 February 2009
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	==About this Structure==		==About this Structure==
-	3AZU is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA].	+	3AZU is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZU OCA].

	==Reference==		==Reference==
-	X-ray crystal structure of the two site-specific mutants His35Gln and His35Leu of azurin from Pseudomonas aeruginosa., Nar H, Messerschmidt A, Huber R, van de Kamp M, Canters GW, J Mol Biol. 1991 Mar 20;218(2):427-47. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/1901363 1901363]~~	+	<ref group="xtra">PMID:1901363</ref><references group="xtra"/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Single protein]]
	[[Category: Huber, R.]]		[[Category: Huber, R.]]
	[[Category: Messerschmidt, A.]]		[[Category: Messerschmidt, A.]]
	[[Category: Nar, H.]]		[[Category: Nar, H.]]

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OCA at 15:50, 28 July 2008

2008-07-28T15:50:42Z

←Older revision		Revision as of 15:50, 28 July 2008
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-	[[Image:3azu.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3azu.png\|left\|200px]]

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	{{STRUCTURE_3azu\| PDB=3azu \| SCENE= }}		{{STRUCTURE_3azu\| PDB=3azu \| SCENE= }}

-	~~'''~~X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA~~'''~~	+	===X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA===


-	~~==Overview==~~	+	<!--
-	~~The three~~-~~dimensional structures of two site~~-specific mutants of the blue copper protein azurin from Pseudomonas aeruginosa have been solved by a combination of isomorphous replacement and Patterson search techniques, and refined by energy-restrained least-squares methods. The ~~mutations introduced by recombinant DNA techniques involve residue His35~~, ~~which was exchanged for glutamine and leucine~~, to ~~probe for its suggested role in electron transfer. The two mutants, His35Gln (H35Q) and His35Leu (H35L), crystallize non-isomorphously in~~ the ~~orthorhombic space group P2~~(1)2(1)2(1) with unit cell dimensions of a = 109.74 A, b = 99.15 A, c = 47.82 A for H35Q, a = 57.82 A, b = 81.06 A, c = 110.03 A for H35L. In each crystal form, there are four molecules in the asymmetric unit. They are arranged as ~~a dimer of dimers in the H35Q case and are distorted from ideal C2 symmetry in H35L~~. ~~The final crystallographic R-value is 16~~.~~3% for 20.747 reflections to a resolution of 2.1 A for H35Q and 17.0% for 32~~,~~548 reflections to 1.9 A for H35L. The crystal structures reported here represent the first crystallographically refined structures for azurin from P. aeruginosa. The structure~~ is very similar to that of azurin from Alcaligenes denitrificans. The copper atom is located about 7 A below a hydrophobic surface region and is ligated by five donor groups in a distorted trigonal bipyramidal fashion. The implications for electron transfer properties of the ~~protein are discussed in terms of the mutation site and the packing of the molecules within the tetramer~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_1901363}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 1901363 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_1901363}}

	==About this Structure==		==About this Structure==
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	[[Category: Messerschmidt, A.]]		[[Category: Messerschmidt, A.]]
	[[Category: Nar, H.]]		[[Category: Nar, H.]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 20~~:19:55 2008''	+
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