3b6a - Revision history

OCA at 12:02, 30 August 2023

2023-08-30T12:02:20Z

←Older revision		Revision as of 12:02, 30 August 2023
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	<StructureSection load='3b6a' size='340' side='right'caption='[[3b6a]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='3b6a' size='340' side='right'caption='[[3b6a]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces~~ coelicolor~~" (muller 1908) lieske 1921~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B6A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B6A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.05Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2opt\|2opt]], [[3b6c\|3b6c]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [https://pdbe.org/3b6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [https://www.ebi.ac.uk/pdbsum/3b6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [https://pdbe.org/3b6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [https://www.ebi.ac.uk/pdbsum/3b6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6a ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q53901_STRCH Q53901_STRCH]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Junop, M S~~]]	+	[[Category: Streptomyces coelicolor]]
-	[[Category: ~~Willems, A R~~]]	+	[[Category: Junop MS]]
-	[[Category: ~~Actinorhodin]]~~	+	[[Category: Willems AR]]
-	~~[[Category: Dna-binding protein]]~~	+
-	~~[[Category: Ligand]]~~	+
-	~~[[Category: Tetr family]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Transcriptional repressor~~]]	+

OCA at 11:24, 5 January 2022

2022-01-05T11:24:55Z

←Older revision		Revision as of 11:24, 5 January 2022
Line 1:		Line 1:

	==Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin==		==Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin==
-	<StructureSection load='3b6a' size='340' side='right' caption='[[3b6a]], [[Resolution\|resolution]] 3.05Å' scene=''>	+	<StructureSection load='3b6a' size='340' side='right'caption='[[3b6a]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3B6A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B6A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2opt\|2opt]], [[3b6c\|3b6c]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2opt\|2opt]], [[3b6c\|3b6c]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [~~http~~://pdbe.org/3b6a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3b6a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [https://pdbe.org/3b6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [https://www.ebi.ac.uk/pdbsum/3b6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b6a_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b6a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3b6a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3b6a" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Junop, M S]]		[[Category: Junop, M S]]
	[[Category: Willems, A R]]		[[Category: Willems, A R]]

OCA at 07:56, 25 October 2017

2017-10-25T07:56:07Z

←Older revision		Revision as of 07:56, 25 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin==		==Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin==
	<StructureSection load='3b6a' size='340' side='right' caption='[[3b6a]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='3b6a' size='340' side='right' caption='[[3b6a]], [[Resolution\|resolution]] 3.05Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2opt\|2opt]], [[3b6c\|3b6c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2opt\|2opt]], [[3b6c\|3b6c]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [http://pdbe.org/3b6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [http://www.ebi.ac.uk/pdbsum/3b6a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [http://pdbe.org/3b6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [http://www.ebi.ac.uk/pdbsum/3b6a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 15:24, 9 February 2016

2016-02-09T15:24:56Z

←Older revision		Revision as of 15:24, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3b6a' size='340' side='right' caption='[[3b6a]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='3b6a' size='340' side='right' caption='[[3b6a]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_coelicolor Streptomyces~~ coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B6A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B6A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2opt\|2opt]], [[3b6c\|3b6c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2opt\|2opt]], [[3b6c\|3b6c]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 ~~Streptomyces~~ coelicolor])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [http://www.ebi.ac.uk/pdbsum/3b6a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [http://pdbe.org/3b6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [http://www.ebi.ac.uk/pdbsum/3b6a PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b6a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3b6a" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces coelicolor]]
	[[Category: Junop, M S]]		[[Category: Junop, M S]]
	[[Category: Willems, A R]]		[[Category: Willems, A R]]

OCA at 09:33, 20 January 2015

2015-01-20T09:33:48Z

←Older revision		Revision as of 09:33, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B6A FirstGlance]. <br>		<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B6A FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2opt\|2opt]], [[3b6c\|3b6c]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2opt\|2opt]], [[3b6c\|3b6c]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [http://www.ebi.ac.uk/pdbsum/3b6a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [http://www.ebi.ac.uk/pdbsum/3b6a PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
-	[[Category: Junop, M S.]]	+	[[Category: Junop, M S]]
-	[[Category: Willems, A R.]]	+	[[Category: Willems, A R]]
	[[Category: Actinorhodin]]		[[Category: Actinorhodin]]
	[[Category: Dna-binding protein]]		[[Category: Dna-binding protein]]

OCA at 05:32, 2 October 2014

2014-10-02T05:32:56Z

←Older revision		Revision as of 05:32, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~3b6a.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin==
		+	<StructureSection load='3b6a' size='340' side='right' caption='[[3b6a]], [[Resolution\|resolution]] 3.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B6A FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2opt\|2opt]], [[3b6c\|3b6c]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">actR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [http://www.ebi.ac.uk/pdbsum/3b6a PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b6a_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Actinorhodin, an antibiotic produced by Streptomyces coelicolor, is exported from the cell by the ActA efflux pump. actA is divergently transcribed from actR, which encodes a TetR-like transcriptional repressor. We showed previously that ActR represses transcription by binding to an operator from the actA/actR intergenic region. Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can cause ActR to dissociate from its operator, leading to derepression. This suggests that ActR may mediate timely self-resistance to an endogenously produced antibiotic by responding to one of its biosynthetic precursors. Here, we report the structural basis for this precursor-mediated derepression with crystal structures of homodimeric ActR by itself and in complex with either actinorhodin or the actinorhodin biosynthetic intermediate (S)-DNPA [4-dihydro-9-hydroxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-aceti c acid]. The ligand-binding tunnel in each ActR monomer has a striking hydrophilic/hydrophobic/hydrophilic arrangement of surface residues that accommodate either one hexacyclic actinorhodin molecule or two back-to-back tricyclic (S)-DNPA molecules. Moreover, our work also reveals the strongest structural evidence to date that TetR-mediated antibiotic resistance may have been acquired from an antibiotic-producer organism.

-	~~{{STRUCTURE_3b6a\| PDB=3b6a \| SCENE= }}~~	+	Crystal structures of the Streptomyces coelicolor TetR-like protein ActR alone and in complex with actinorhodin or the actinorhodin biosynthetic precursor (S)-DNPA.,Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR J Mol Biol. 2008 Mar 7;376(5):1377-87. Epub 2008 Jan 4. PMID:18207163<ref>PMID:18207163</ref>

-	~~===Crystal structure~~ of the ~~Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18207163}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3b6a]] is a 8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018207163<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Junop, M S.]]		[[Category: Junop, M S.]]

OCA: Protected "3b6a" [edit=sysop:move=sysop]

2013-01-07T18:53:01Z

Protected "3b6a" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:53, 7 January 2013

OCA at 18:52, 7 January 2013

2013-01-07T18:52:57Z

←Older revision		Revision as of 18:52, 7 January 2013
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-	{{Seed}}
	[[Image:3b6a.png\|left\|200px]]		[[Image:3b6a.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3b6a", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3b6a\| PDB=3b6a \| SCENE= }}		{{STRUCTURE_3b6a\| PDB=3b6a \| SCENE= }}

	===Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin===		===Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18207163}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18207163 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18207163}}		{{ABSTRACT_PUBMED_18207163}}

	==About this Structure==		==About this Structure==
-	~~3B6A~~ is a 8 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA].	+	[[3b6a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18207163~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018207163</ref><references group="xtra"/>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Junop, M S.]]		[[Category: Junop, M S.]]
Line 31:		Line 19:
	[[Category: Ligand]]		[[Category: Ligand]]
	[[Category: Tetr family]]		[[Category: Tetr family]]
		+	[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
	[[Category: Transcriptional repressor]]		[[Category: Transcriptional repressor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:48:32 2009''

OCA at 08:48, 17 February 2009

2009-02-17T08:48:33Z

←Older revision		Revision as of 08:48, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3B6A is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA].	+	3B6A is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA].

	==Reference==		==Reference==
-	Crystal structures of the Streptomyces coelicolor TetR-like protein ActR alone and in complex with actinorhodin or the actinorhodin biosynthetic precursor (S)-DNPA., Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR, J Mol Biol. 2008 Mar 7;376(5):1377-87. Epub 2008 Jan 4. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18207163 18207163]~~	+	<ref group="xtra">PMID:18207163</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Junop, M S.]]		[[Category: Junop, M S.]]
Line 35:		Line 34:
	[[Category: Transcriptional repressor]]		[[Category: Transcriptional repressor]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 14~~:46:32 ~~2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:48:32 2009''

OCA at 11:46, 28 July 2008

2008-07-28T11:46:35Z

←Older revision		Revision as of 11:46, 28 July 2008
Line 1:		Line 1:
-	[[Image:3b6a.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3b6a.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_3b6a\| PDB=3b6a \| SCENE= }}		{{STRUCTURE_3b6a\| PDB=3b6a \| SCENE= }}

-	~~'''~~Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin~~'''~~	+	===Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin===


-	~~==Overview==~~	+	<!--
-	~~Actinorhodin~~, ~~an antibiotic produced by Streptomyces coelicolor~~, ~~is exported from~~ the ~~cell by the ActA efflux pump. actA is divergently transcribed from actR, which encodes a TetR-like transcriptional repressor. We showed previously that ActR represses transcription by binding~~ to ~~an operator from~~ the ~~actA~~/~~actR intergenic region~~. ~~Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can cause ActR to dissociate from its operator, leading to derepression~~. ~~This suggests that ActR may mediate timely self-resistance to an endogenously produced antibiotic by responding to one of its biosynthetic precursors. Here~~, ~~we report~~ the structural basis for this precursor-mediated derepression with crystal structures of homodimeric ActR by itself and in complex with either actinorhodin or the actinorhodin biosynthetic intermediate (S)-DNPA [4-dihydro-9-hydroxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-aceti c acid]. ~~The ligand~~-~~binding tunnel in each ActR monomer has a striking hydrophilic/hydrophobic/hydrophilic arrangement of surface residues that accommodate either one hexacyclic actinorhodin molecule or two back~~-to-back tricyclic (S)-DNPA molecules. Moreover, our work also reveals the strongest structural evidence to date that TetR-mediated antibiotic resistance may have been acquired from an antibiotic-producer organism.	+	The line below this paragraph, {{ABSTRACT_PUBMED_18207163}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18207163 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18207163}}

	==About this Structure==		==About this Structure==
Line 30:		Line 34:
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
	[[Category: Transcriptional repressor]]		[[Category: Transcriptional repressor]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 20~~:25:58 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:46:32 2008''