3b8h - Revision history

OCA at 12:04, 30 August 2023

2023-08-30T12:04:46Z

←Older revision		Revision as of 12:04, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='3b8h' size='340' side='right'caption='[[3b8h]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3b8h' size='340' side='right'caption='[[3b8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b8h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B8H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b8h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B8H FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAD:NICOTINAMIDE~~-~~ADENINE-DINUCLEOTIDE'>NAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;~~'>~~[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[1zm9\|1zm9]], [[3b78\|3b78]], [[3b82\|3b82]]</div></td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eta ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/~~NAD~~(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8h OCA], [https://pdbe.org/3b8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b8h RCSB], [https://www.ebi.ac.uk/pdbsum/3b8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b8h ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8h OCA], [https://pdbe.org/3b8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b8h RCSB], [https://www.ebi.ac.uk/pdbsum/3b8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b8h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~TOXA_PSEAE TOXA_PSEAE~~]] An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.<ref>PMID:18276581</ref>	+	[https://www.uniprot.org/uniprot/EF2_YEAST EF2_YEAST]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 41:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Jorgensen, R]]	+	[[Category: Jorgensen R]]
-	[[Category: Merrill~~, A R]]~~	+	[[Category: Merrill AR]]
-	~~[[Category: Adp-ribosylation]]~~	+
-	~~[[Category: Biosynthetic protein-transferase complex]]~~	+
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Elongation factor]]~~	+
-	~~[[Category: Glycosyltransferase]]~~	+
-	~~[[Category: Gtp-binding]]~~	+
-	~~[[Category: Nad]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Phosphorylation]]~~	+
-	~~[[Category: Protein biosynthesis]]~~	+
-	~~[[Category: Rna-binding]]~~	+
-	~~[[Category: Rrna-binding]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Toxin-substrate complex]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 18:54, 20 October 2021

2021-10-20T18:54:59Z

←Older revision		Revision as of 18:54, 20 October 2021
Line 1:		Line 1:

	==Structure of the eEF2-ExoA(E546A)-NAD+ complex==		==Structure of the eEF2-ExoA(E546A)-NAD+ complex==
-	<StructureSection load='3b8h' size='340' side='right' caption='[[3b8h]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3b8h' size='340' side='right'caption='[[3b8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b8h]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885] and [~~http~~://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3B8H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b8h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B8H FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[1zm9\|1zm9]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[1zm9\|1zm9]], [[3b78\|3b78]], [[3b82\|3b82]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eta ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eta ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8h OCA], [~~http~~://pdbe.org/3b8h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3b8h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3b8h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3b8h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8h OCA], [https://pdbe.org/3b8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b8h RCSB], [https://www.ebi.ac.uk/pdbsum/3b8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b8h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TOXA_PSEAE TOXA_PSEAE]] An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.<ref>PMID:18276581</ref>	+	[[https://www.uniprot.org/uniprot/TOXA_PSEAE TOXA_PSEAE]] An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.<ref>PMID:18276581</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:

	==See Also==		==See Also==
-	*[[Elongation factor\|Elongation factor]]	+	*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
-	*[[Exotoxin\|Exotoxin]]	+	*[[Exotoxin 3D structures\|Exotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Jorgensen, R]]		[[Category: Jorgensen, R]]

OCA at 08:05, 31 October 2018

2018-10-31T08:05:23Z

←Older revision		Revision as of 08:05, 31 October 2018
Line 1:		Line 1:
		+
	==Structure of the eEF2-ExoA(E546A)-NAD+ complex==		==Structure of the eEF2-ExoA(E546A)-NAD+ complex==
	<StructureSection load='3b8h' size='340' side='right' caption='[[3b8h]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3b8h' size='340' side='right' caption='[[3b8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 8:		Line 9:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eta ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eta ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8h OCA], [http://pdbe.org/3b8h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b8h RCSB], [http://www.ebi.ac.uk/pdbsum/3b8h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8h OCA], [http://pdbe.org/3b8h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b8h RCSB], [http://www.ebi.ac.uk/pdbsum/3b8h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b8h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/3b8h_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/3b8h_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 44:		Line 45:
	[[Category: Adp-ribosylation]]		[[Category: Adp-ribosylation]]
	[[Category: Biosynthetic protein-transferase complex]]		[[Category: Biosynthetic protein-transferase complex]]
		+	[[Category: Cytoplasm]]
	[[Category: Elongation factor]]		[[Category: Elongation factor]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]

OCA at 01:46, 10 February 2016

2016-02-10T01:46:56Z

←Older revision		Revision as of 01:46, 10 February 2016
Line 2:		Line 2:
	<StructureSection load='3b8h' size='340' side='right' caption='[[3b8h]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3b8h' size='340' side='right' caption='[[3b8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b8h]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B8H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b8h]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B8H FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[1zm9\|1zm9]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[1zm9\|1zm9]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eta ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 ~~Pseudomonas aeruginosa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eta ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b8h RCSB], [http://www.ebi.ac.uk/pdbsum/3b8h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8h OCA], [http://pdbe.org/3b8h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b8h RCSB], [http://www.ebi.ac.uk/pdbsum/3b8h PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b8h ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 30:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3b8h" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 38:		Line 39:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Jorgensen, R]]		[[Category: Jorgensen, R]]

OCA at 10:56, 25 December 2014

2014-12-25T10:56:55Z

←Older revision		Revision as of 10:56, 25 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3b8h.~~png~~\|~~left~~\|200px]]	+	==Structure of the eEF2-ExoA(E546A)-NAD+ complex==
		+	<StructureSection load='3b8h' size='340' side='right' caption='[[3b8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3b8h]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B8H FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[1zm9\|1zm9]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eta ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b8h RCSB], [http://www.ebi.ac.uk/pdbsum/3b8h PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TOXA_PSEAE TOXA_PSEAE]] An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.<ref>PMID:18276581</ref>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/3b8h_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Exotoxin A (ExoA) from Pseudomonas aeruginosa is an important virulence factor that belongs to a class of exotoxins that are secreted by pathogenic bacteria which cause human diseases such as cholera, diphtheria, pneumonia and whooping cough. We present the first crystal structures, to our knowledge, of ExoA in complex with elongation factor 2 (eEF2) and intact NAD(+), which indicate a direct role of two active-site loops in ExoA during the catalytic cycle. One loop moves to form a solvent cover for the active site of the enzyme and reaches towards the target residue (diphthamide) in eEF2 forming an important hydrogen bond. The NAD(+) substrate adopts a conformation remarkably different from that of the NAD(+) analogue, betaTAD, observed in previous structures, and fails to trigger any loop movements. Mutational studies of the two loops in the toxin identify several residues important for catalytic activity, in particular Glu 546 and Arg 551, clearly supporting the new complex structures. On the basis of these data, we propose a transition-state model for the toxin-catalysed reaction.

-	~~{{STRUCTURE_3b8h\| PDB=3b8h \| SCENE= }}~~	+	The nature and character of the transition state for the ADP-ribosyltransferase reaction.,Jorgensen R, Wang Y, Visschedyk D, Merrill AR EMBO Rep. 2008 Aug;9(8):802-9. Epub 2008 Jun 27. PMID:18583986<ref>PMID:18583986</ref>

-	~~===Structure~~ of the ~~eEF2-ExoA(E546A)-NAD+ complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18583986}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3b8h]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en~~.~~wikipedia.org~~/~~wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA].~~	+

	==See Also==		==See Also==
	*[[Elongation factor\|Elongation factor]]		*[[Elongation factor\|Elongation factor]]
-		+	*[[Exotoxin\|Exotoxin]]
-	==~~Reference~~==	+	== References ==
-	<~~ref group="xtra">PMID:018583986<~~/~~ref~~><~~references group="xtra"~~/>	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Jorgensen, R.]]	+	[[Category: Jorgensen, R]]
-	[[Category: Merrill, A R.]]	+	[[Category: Merrill, A R]]
	[[Category: Adp-ribosylation]]		[[Category: Adp-ribosylation]]
	[[Category: Biosynthetic protein-transferase complex]]		[[Category: Biosynthetic protein-transferase complex]]

OCA: Protected "3b8h" [edit=sysop:move=sysop]

2012-10-20T15:53:27Z

Protected "3b8h" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:53, 20 October 2012

OCA at 15:53, 20 October 2012

2012-10-20T15:53:24Z

←Older revision		Revision as of 15:53, 20 October 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3b8h.png\|left\|200px]]		[[Image:3b8h.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3b8h", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3b8h\| PDB=3b8h \| SCENE= }}		{{STRUCTURE_3b8h\| PDB=3b8h \| SCENE= }}

	===Structure of the eEF2-ExoA(E546A)-NAD+ complex===		===Structure of the eEF2-ExoA(E546A)-NAD+ complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18583986}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18583986 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18583986}}		{{ABSTRACT_PUBMED_18583986}}

	==About this Structure==		==About this Structure==
-	~~3B8H~~ is a 6 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA].	+	[[3b8h]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA].
		+
		+	==See Also==
		+	*[[Elongation factor\|Elongation factor]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18583986~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018583986</ref><references group="xtra"/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
Line 29:		Line 20:
	[[Category: Merrill, A R.]]		[[Category: Merrill, A R.]]
	[[Category: Adp-ribosylation]]		[[Category: Adp-ribosylation]]
-	[[Category: Biosynthetic protein/transferase complex]]	+	[[Category: Biosynthetic protein-transferase complex]]
-	~~[[Category: Cytoplasm~~]]	+
	[[Category: Elongation factor]]		[[Category: Elongation factor]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
Line 43:		Line 33:
	[[Category: Toxin-substrate complex]]		[[Category: Toxin-substrate complex]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 09:20:27 2009''

OCA at 06:20, 6 May 2009

2009-05-06T06:20:28Z

←Older revision		Revision as of 06:20, 6 May 2009
Line 12:		Line 12:
	===Structure of the eEF2-ExoA(E546A)-NAD+ complex===		===Structure of the eEF2-ExoA(E546A)-NAD+ complex===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18583986}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18583986 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18583986}}

	==About this Structure==		==About this Structure==
	3B8H is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA].		3B8H is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:18583986</ref><references group="xtra"/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
Line 35:		Line 44:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 14~~:25:51 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 09:20:27 2009''

OCA at 12:25, 17 February 2009

2009-02-17T12:25:52Z

←Older revision		Revision as of 12:25, 17 February 2009
Line 1:		Line 1:
-	[[Image:3b8h.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3b8h.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_3b8h\| PDB=3b8h \| SCENE= }}		{{STRUCTURE_3b8h\| PDB=3b8h \| SCENE= }}

-	~~'''~~Structure of the eEF2-ExoA(E546A)-NAD+ complex~~'''~~	+	===Structure of the eEF2-ExoA(E546A)-NAD+ complex===


	==About this Structure==		==About this Structure==
-	3B8H is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA].	+	3B8H is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA].
-	~~[[Category: Protein complex]]~~	+
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
Line 34:		Line 34:
	[[Category: Toxin-substrate complex]]		[[Category: Toxin-substrate complex]]
	[[Category: Transferase]]		[[Category: Transferase]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jun 18 12~~:08:~~13 2008~~''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:25:51 2009''

OCA at 09:08, 18 June 2008

2008-06-18T09:08:14Z

←Older revision		Revision as of 09:08, 18 June 2008
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3b8h.jpg\|left\|200px]]

-	The ~~entry 3b8h~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3b8h", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3b8h\| PDB=3b8h \| SCENE= }}

-	~~Authors: Jorgensen, R., Merrill, A.R.~~	+	'''Structure of the eEF2-ExoA(E546A)-NAD+ complex'''

-	Description: Structure of the eEF2-ExoA(E546A)-NAD+ complex

-		+	==About this Structure==
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun ~~11 09~~:24:36 2008''	+	3B8H is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8H OCA].
		+	[[Category: Protein complex]]
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Jorgensen, R.]]
		+	[[Category: Merrill, A R.]]
		+	[[Category: Adp-ribosylation]]
		+	[[Category: Biosynthetic protein/transferase complex]]
		+	[[Category: Cytoplasm]]
		+	[[Category: Elongation factor]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Gtp-binding]]
		+	[[Category: Nad]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Phosphorylation]]
		+	[[Category: Protein biosynthesis]]
		+	[[Category: Rna-binding]]
		+	[[Category: Rrna-binding]]
		+	[[Category: Toxin]]
		+	[[Category: Toxin-substrate complex]]
		+	[[Category: Transferase]]
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:08:13 2008''