3bs7 - Revision history

OCA at 09:30, 21 February 2024

2024-02-21T09:30:59Z

←Older revision		Revision as of 09:30, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3bs7' size='340' side='right'caption='[[3bs7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3bs7' size='340' side='right'caption='[[3bs7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bs7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BS7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bs7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BS7 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3bs5\|3bs5]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">ave ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=7227 DROME])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [https://pdbe.org/3bs7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [https://www.ebi.ac.uk/pdbsum/3bs7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [https://pdbe.org/3bs7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [https://www.ebi.ac.uk/pdbsum/3bs7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AVE_DROME AVE_DROME]] Required for normal photoreceptor differentiation between Ras and Raf for EGFR signaling in the eye and for mitogen-activated protein kinase phosphorylation. Probably acts together with Cnk to promote Raf activation, perhaps by recruiting an activating kinase.	+	[https://www.uniprot.org/uniprot/AVE_DROME AVE_DROME] Required for normal photoreceptor differentiation between Ras and Raf for EGFR signaling in the eye and for mitogen-activated protein kinase phosphorylation. Probably acts together with Cnk to promote Raf activation, perhaps by recruiting an activating kinase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bs7 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bs7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	RAF kinase functions in the mitogen-activated protein kinase (MAPK) pathway to transmit growth signals to the downstream kinases MEK and ERK. Activation of RAF catalytic activity is facilitated by a regulatory complex comprising the proteins CNK (Connector enhancer of KSR), HYP (Hyphen), and KSR (Kinase Suppressor of Ras). The sterile alpha-motif (SAM) domain found in both CNK and HYP plays an essential role in complex formation. Here, we have determined the x-ray crystal structure of the SAM domain of CNK in complex with the SAM domain of HYP. The structure reveals a single-junction SAM domain dimer of 1:1 stoichiometry in which the binding mode is a variation of polymeric SAM domain interactions. Through in vitro and in vivo mutational analyses, we show that the specific mode of dimerization revealed by the crystal structure is essential for RAF signaling and facilitates the recruitment of KSR to form the CNK/HYP/KSR regulatory complex. We present two docking-site models to account for how SAM domain dimerization might influence the formation of a higher-order CNK/HYP/KSR complex.
-
-	CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling.,Rajakulendran T, Sahmi M, Kurinov I, Tyers M, Therrien M, Sicheri F Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2836-41. Epub 2008 Feb 19. PMID:18287031<ref>PMID:18287031</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3bs7" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Rajakulendran, T]]	+	[[Category: Rajakulendran T]]
-	[[Category: Sicheri, F]]	+	[[Category: Sicheri F]]
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Sensory transduction]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Vision~~]]	+

OCA at 08:23, 19 January 2022

2022-01-19T08:23:51Z

←Older revision		Revision as of 08:23, 19 January 2022
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	==Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle==		==Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle==
-	<StructureSection load='3bs7' size='340' side='right' caption='[[3bs7]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3bs7' size='340' side='right'caption='[[3bs7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bs7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3BS7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bs7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BS7 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bs5\|3bs5]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bs5\|3bs5]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ave ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ave ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [~~http~~://pdbe.org/3bs7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3bs7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [https://pdbe.org/3bs7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [https://www.ebi.ac.uk/pdbsum/3bs7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AVE_DROME AVE_DROME]] Required for normal photoreceptor differentiation between Ras and Raf for EGFR signaling in the eye and for mitogen-activated protein kinase phosphorylation. Probably acts together with Cnk to promote Raf activation, perhaps by recruiting an activating kinase.	+	[[https://www.uniprot.org/uniprot/AVE_DROME AVE_DROME]] Required for normal photoreceptor differentiation between Ras and Raf for EGFR signaling in the eye and for mitogen-activated protein kinase phosphorylation. Probably acts together with Cnk to promote Raf activation, perhaps by recruiting an activating kinase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Drome]]		[[Category: Drome]]
		+	[[Category: Large Structures]]
	[[Category: Rajakulendran, T]]		[[Category: Rajakulendran, T]]
	[[Category: Sicheri, F]]		[[Category: Sicheri, F]]

OCA at 08:04, 31 October 2018

2018-10-31T08:04:14Z

←Older revision		Revision as of 08:04, 31 October 2018
Line 1:		Line 1:
		+
	==Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle==		==Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle==
	<StructureSection load='3bs7' size='340' side='right' caption='[[3bs7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3bs7' size='340' side='right' caption='[[3bs7]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bs5\|3bs5]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bs5\|3bs5]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ave ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ave ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [http://pdbe.org/3bs7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [http://www.ebi.ac.uk/pdbsum/3bs7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [http://pdbe.org/3bs7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [http://www.ebi.ac.uk/pdbsum/3bs7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bs7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bs7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 36:
	[[Category: Rajakulendran, T]]		[[Category: Rajakulendran, T]]
	[[Category: Sicheri, F]]		[[Category: Sicheri, F]]
		+	[[Category: Cytoplasm]]
	[[Category: Membrane]]		[[Category: Membrane]]
	[[Category: Sensory transduction]]		[[Category: Sensory transduction]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Vision]]		[[Category: Vision]]

OCA at 01:23, 9 February 2016

2016-02-09T01:23:01Z

←Older revision		Revision as of 01:23, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3bs7' size='340' side='right' caption='[[3bs7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3bs7' size='340' side='right' caption='[[3bs7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bs7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Drosophila_melanogaster Drosophila melanogaster~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BS7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bs7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BS7 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bs5\|3bs5]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bs5\|3bs5]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ave ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 ~~Drosophila melanogaster~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ave ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [http://www.ebi.ac.uk/pdbsum/3bs7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [http://pdbe.org/3bs7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [http://www.ebi.ac.uk/pdbsum/3bs7 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bs7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3bs7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drosophila melanogaster~~]]	+	[[Category: Drome]]
	[[Category: Rajakulendran, T]]		[[Category: Rajakulendran, T]]
	[[Category: Sicheri, F]]		[[Category: Sicheri, F]]

OCA at 03:28, 25 December 2014

2014-12-25T03:28:11Z

←Older revision		Revision as of 03:28, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3bs7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BS7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3bs7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BS7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bs5\|3bs5]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bs5\|3bs5]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ave ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ave ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [http://www.ebi.ac.uk/pdbsum/3bs7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [http://www.ebi.ac.uk/pdbsum/3bs7 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AVE_DROME AVE_DROME]] Required for normal photoreceptor differentiation between Ras and Raf for EGFR signaling in the eye and for mitogen-activated protein kinase phosphorylation. Probably acts together with Cnk to promote Raf activation, perhaps by recruiting an activating kinase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
-	[[Category: Rajakulendran, T.]]	+	[[Category: Rajakulendran, T]]
-	[[Category: Sicheri, F.]]	+	[[Category: Sicheri, F]]
	[[Category: Membrane]]		[[Category: Membrane]]
	[[Category: Sensory transduction]]		[[Category: Sensory transduction]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Vision]]		[[Category: Vision]]

OCA at 21:20, 2 October 2014

2014-10-02T21:20:16Z

←Older revision		Revision as of 21:20, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~3bs7.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle==
		+	<StructureSection load='3bs7' size='340' side='right' caption='[[3bs7]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3bs7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BS7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bs5\|3bs5]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ave ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bs7 RCSB], [http://www.ebi.ac.uk/pdbsum/3bs7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bs7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RAF kinase functions in the mitogen-activated protein kinase (MAPK) pathway to transmit growth signals to the downstream kinases MEK and ERK. Activation of RAF catalytic activity is facilitated by a regulatory complex comprising the proteins CNK (Connector enhancer of KSR), HYP (Hyphen), and KSR (Kinase Suppressor of Ras). The sterile alpha-motif (SAM) domain found in both CNK and HYP plays an essential role in complex formation. Here, we have determined the x-ray crystal structure of the SAM domain of CNK in complex with the SAM domain of HYP. The structure reveals a single-junction SAM domain dimer of 1:1 stoichiometry in which the binding mode is a variation of polymeric SAM domain interactions. Through in vitro and in vivo mutational analyses, we show that the specific mode of dimerization revealed by the crystal structure is essential for RAF signaling and facilitates the recruitment of KSR to form the CNK/HYP/KSR regulatory complex. We present two docking-site models to account for how SAM domain dimerization might influence the formation of a higher-order CNK/HYP/KSR complex.

-	~~{{STRUCTURE_3bs7\| PDB=3bs7 \| SCENE= }}~~	+	CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling.,Rajakulendran T, Sahmi M, Kurinov I, Tyers M, Therrien M, Sicheri F Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2836-41. Epub 2008 Feb 19. PMID:18287031<ref>PMID:18287031</ref>

-	~~===Crystal structure~~ of the ~~Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18287031}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3bs7]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Drosophila_melanogaster Drosophila melanogaster]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018287031<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Rajakulendran, T.]]		[[Category: Rajakulendran, T.]]

OCA: Protected "3bs7" [edit=sysop:move=sysop]

2013-01-07T18:52:00Z

Protected "3bs7" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:52, 7 January 2013

OCA at 18:51, 7 January 2013

2013-01-07T18:51:58Z

←Older revision		Revision as of 18:51, 7 January 2013
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-	{{Seed}}
	[[Image:3bs7.png\|left\|200px]]		[[Image:3bs7.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3bs7\| PDB=3bs7 \| SCENE= }}		{{STRUCTURE_3bs7\| PDB=3bs7 \| SCENE= }}

	===Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle===		===Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle===

-
-	<!--
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	{{ABSTRACT_PUBMED_18287031}}		{{ABSTRACT_PUBMED_18287031}}

	==About this Structure==		==About this Structure==
-	~~3BS7~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA].	+	[[3bs7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18287031~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018287031</ref><references group="xtra"/>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Rajakulendran, T.]]		[[Category: Rajakulendran, T.]]
	[[Category: Sicheri, F.]]		[[Category: Sicheri, F.]]
-	[[Category: Cytoplasm]]
	[[Category: Membrane]]		[[Category: Membrane]]
	[[Category: Sensory transduction]]		[[Category: Sensory transduction]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Vision]]		[[Category: Vision]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:19:20 2009''

OCA at 03:19, 17 February 2009

2009-02-17T03:19:22Z

←Older revision		Revision as of 03:19, 17 February 2009
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	==About this Structure==		==About this Structure==
-	3BS7 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA].	+	3BS7 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA].

	==Reference==		==Reference==
-	CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling., Rajakulendran T, Sahmi M, Kurinov I, Tyers M, Therrien M, Sicheri F, Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2836-41. Epub 2008 Feb 19. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18287031 18287031]~~	+	<ref group="xtra">PMID:18287031</ref><references group="xtra"/>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
-	[[Category: Single protein]]
	[[Category: Rajakulendran, T.]]		[[Category: Rajakulendran, T.]]
	[[Category: Sicheri, F.]]		[[Category: Sicheri, F.]]
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	[[Category: Vision]]		[[Category: Vision]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Aug 20 11~~:30:~~49 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:19:20 2009''

OCA at 08:30, 20 August 2008

2008-08-20T08:30:49Z

←Older revision		Revision as of 08:30, 20 August 2008
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-	[[Image:3bs7.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3bs7.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_3bs7\| PDB=3bs7 \| SCENE= }}		{{STRUCTURE_3bs7\| PDB=3bs7 \| SCENE= }}

-	~~'''~~Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle~~'''~~	+	===Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18287031}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18287031 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18287031}}

	==About this Structure==		==About this Structure==
	3BS7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA].		3BS7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS7 OCA].
		+
		+	==Reference==
		+	CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling., Rajakulendran T, Sahmi M, Kurinov I, Tyers M, Therrien M, Sicheri F, Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2836-41. Epub 2008 Feb 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18287031 18287031]
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Single protein]]		[[Category: Single protein]]
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	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Vision]]		[[Category: Vision]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 21~~:03:37 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 20 11:30:49 2008''