3c4m - Revision history

OCA at 12:22, 30 August 2023

2023-08-30T12:22:11Z

←Older revision		Revision as of 12:22, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='3c4m' size='340' side='right'caption='[[3c4m]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3c4m' size='340' side='right'caption='[[3c4m]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3c4m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C4M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3c4m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C4M FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GLC:ALPHA~~-~~D-GLUCOSE'>GLC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">malE, PTHR1, PTHR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4m OCA], [https://pdbe.org/3c4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c4m RCSB], [https://www.ebi.ac.uk/pdbsum/3c4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c4m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4m OCA], [https://pdbe.org/3c4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c4m RCSB], [https://www.ebi.ac.uk/pdbsum/3c4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c4m ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/~~PTHY_HUMAN PTHY_HUMAN~~]~~] Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:[https://omim~~.~~org/~~entry~~/146200 146200]]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia~~. ~~FIH~~ is ~~characterized~~ by ~~hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone~~. ~~Symptoms are seizures, tetany and cramps~~. ~~FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism~~.~~<ref>PMID:2212001</ref> <ref>PMID:10523031</ref> <ref>PMID:18056632</ref>~~	+	[https://www.uniprot.org/uniprot/PTH1R_HUMAN PTH1R_HUMAN] Blomstrand lethal chondrodysplasia;Dental ankylosis;Eiken syndrome;Metaphyseal chondrodysplasia, Jansen type;Enchondromatosis. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease may be caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides. [[https://www.uniprot.org/uniprot/~~PTHY_HUMAN PTHY_HUMAN~~]~~] PTH elevates calcium level by dissolving the salts in bone~~ and ~~preventing their renal excretion. Stimulates [1~~-~~14C]-2-deoxy-D-glucose (2DG) transport~~ and ~~glycogen synthesis in osteoblastic cells~~.<ref>PMID:~~21076856~~</ref>	+	[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/PTH1R_HUMAN PTH1R_HUMAN] This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.<ref>PMID:18611381</ref> <ref>PMID:20172855</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Escherichia coli]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pioszak~~, A A~~]]	+	[[Category: Pioszak AA]]
-	[[Category: Xu~~, H E]]~~	+	[[Category: Xu HE]]
-	~~[[Category: Cleavage on pair of basic residue]]~~	+
-	~~[[Category: Disease mutation]]~~	+
-	~~[[Category: Dwarfism]]~~	+
-	~~[[Category: G-protein-coupled receptor]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Parathyroid hormone]]~~	+
-	~~[[Category: Receptor]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Sugar transport]]~~	+
-	~~[[Category: Transducer]]~~	+
-	~~[[Category: Transmembrane]]~~	+
-	~~[[Category: Transport~~]]	+

OCA at 07:43, 27 January 2022

2022-01-27T07:43:00Z

←Older revision		Revision as of 07:43, 27 January 2022
Line 1:		Line 1:

	==Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)==		==Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)==
-	<StructureSection load='3c4m' size='340' side='right' caption='[[3c4m]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='3c4m' size='340' side='right'caption='[[3c4m]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3c4m]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3C4M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3c4m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C4M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MAL~~:~~MALTOSE~~'>~~MAL~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE, PTHR1, PTHR ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE, PTHR1, PTHR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3c4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4m OCA], [~~http~~://pdbe.org/3c4m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3c4m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3c4m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3c4m ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4m OCA], [https://pdbe.org/3c4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c4m RCSB], [https://www.ebi.ac.uk/pdbsum/3c4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c4m ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:[~~http~~://omim.org/entry/146200 146200]]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.<ref>PMID:2212001</ref> <ref>PMID:10523031</ref> <ref>PMID:18056632</ref>	+	[[https://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:[https://omim.org/entry/146200 146200]]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.<ref>PMID:2212001</ref> <ref>PMID:10523031</ref> <ref>PMID:18056632</ref>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides. [[~~http~~://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.<ref>PMID:21076856</ref>	+	[[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides. [[https://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.<ref>PMID:21076856</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c4/3c4m_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c4/3c4m_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Pioszak, A A]]		[[Category: Pioszak, A A]]
	[[Category: Xu, H E]]		[[Category: Xu, H E]]

OCA at 14:00, 11 August 2016

2016-08-11T14:00:21Z

←Older revision		Revision as of 14:00, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)==		==Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)==
	<StructureSection load='3c4m' size='340' side='right' caption='[[3c4m]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3c4m' size='340' side='right' caption='[[3c4m]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3c4m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C4M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3c4m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C4M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE, PTHR1, PTHR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE, PTHR1, PTHR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c4m RCSB], [http://www.ebi.ac.uk/pdbsum/3c4m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4m OCA], [http://pdbe.org/3c4m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c4m RCSB], [http://www.ebi.ac.uk/pdbsum/3c4m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c4m ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 20:		Line 21:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c4m ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 30:		Line 31:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3c4m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Human]]
	[[Category: Pioszak, A A]]		[[Category: Pioszak, A A]]
	[[Category: Xu, H E]]		[[Category: Xu, H E]]

OCA at 11:48, 20 January 2015

2015-01-20T11:48:08Z

←Older revision		Revision as of 11:48, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3c4m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C4M FirstGlance]. <br>		<table><tr><td colspan='2'>[[3c4m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C4M FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE, PTHR1, PTHR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE, PTHR1, PTHR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c4m RCSB], [http://www.ebi.ac.uk/pdbsum/3c4m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c4m RCSB], [http://www.ebi.ac.uk/pdbsum/3c4m PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:[http://omim.org/entry/146200 146200]]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.<ref>PMID:2212001</ref> <ref>PMID:10523031</ref> <ref>PMID:18056632</ref>		[[http://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:[http://omim.org/entry/146200 146200]]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.<ref>PMID:2212001</ref> <ref>PMID:10523031</ref> <ref>PMID:18056632</ref>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Pioszak, A A.]]	+	[[Category: Pioszak, A A]]
-	[[Category: Xu, H E.]]	+	[[Category: Xu, H E]]
	[[Category: Cleavage on pair of basic residue]]		[[Category: Cleavage on pair of basic residue]]
	[[Category: Disease mutation]]		[[Category: Disease mutation]]

OCA at 21:33, 2 October 2014

2014-10-02T21:33:00Z

←Older revision		Revision as of 21:33, 2 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3c4m\| PDB=3c4m \| SCENE= }}~~	+	==Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)==
-	===Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)===	+	<StructureSection load='3c4m' size='340' side='right' caption='[[3c4m]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~{{ABSTRACT_PUBMED_18375760}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3c4m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C4M FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE, PTHR1, PTHR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c4m RCSB], [http://www.ebi.ac.uk/pdbsum/3c4m PDBsum]</span></td></tr>
		+	<table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:[http://omim.org/entry/146200 146200]]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.<ref>PMID:2212001</ref> <ref>PMID:10523031</ref> <ref>PMID:18056632</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides. [[http://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.<ref>PMID:21076856</ref>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c4/3c4m_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Parathyroid hormone (PTH) is central to calcium homeostasis and bone maintenance in vertebrates, and as such it has been used for treating osteoporosis. It acts primarily by binding to its receptor, PTH1R, a member of the class B G protein-coupled receptor (GPCR) family that also includes receptors for glucagon, calcitonin, and other therapeutically important peptide hormones. Despite considerable interest and much research, determining the structure of the receptor-hormone complex has been hindered by difficulties in purifying the receptor and obtaining diffraction-quality crystals. Here, we present a method for expression and purification of the extracellular domain (ECD) of human PTH1R engineered as a maltose-binding protein (MBP) fusion that readily crystallizes. The 1.95-A structure of PTH bound to the MBP-PTH1R-ECD fusion reveals that PTH docks as an amphipathic helix into a central hydrophobic groove formed by a three-layer alpha-beta-betaalpha fold of the PTH1R ECD, resembling a hot dog in a bun. Conservation in the ECD scaffold and the helical structure of peptide hormones emphasizes this hot dog model as a general mechanism of hormone recognition common to class B GPCRs. Our findings reveal critical insights into PTH actions and provide a rational template for drug design that targets this hormone signaling pathway.

-	~~==Disease==~~	+	Molecular recognition of parathyroid hormone by its G protein-coupled receptor.,Pioszak AA, Xu HE Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5034-9. Epub 2008 Mar 28. PMID:18375760<ref>PMID:18375760</ref>
-	~~[[http://www~~.~~uniprot~~.~~org/uniprot/PTHY_HUMAN PTHY_HUMAN]] Defects in PTH are a cause of familial isolated hypoparathyroidism~~ (~~FIH~~) ~~[MIM~~:~~[http://omim~~.~~org/entry/146200 146200]]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia~~. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.<ref>PMID:~~2212001</ref><ref>PMID:10523031</ref>~~<ref>PMID:~~18056632~~</ref>	+

-	~~==Function==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[http:~~/~~/www~~.~~uniprot~~.~~org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport~~ of and chemotaxis toward maltooligosaccharides. [[http://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.<~~ref~~>~~PMID:21076856~~</~~ref~~>	+	</div>
-		+	== References ==
-	==~~About this Structure==~~	+	<references/>
-	[[3c4m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA].	+	__TOC__
-		+	</StructureSection>
-	~~==Reference~~==	+
-	~~<ref group="xtra">PMID:018375760</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Pioszak, A A.]]		[[Category: Pioszak, A A.]]

OCA at 22:31, 24 March 2013

2013-03-24T22:31:38Z

←Older revision		Revision as of 22:31, 24 March 2013
Line 1:		Line 1:
-	[[Image:3c4m.png\|left\|200px]]
-
	{{STRUCTURE_3c4m\| PDB=3c4m \| SCENE= }}		{{STRUCTURE_3c4m\| PDB=3c4m \| SCENE= }}
-
	===Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)===		===Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)===
		+	{{ABSTRACT_PUBMED_18375760}}

-	~~{{ABSTRACT_PUBMED_18375760}}~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:[http://omim.org/entry/146200 146200]]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.<ref>PMID:2212001</ref><ref>PMID:10523031</ref><ref>PMID:18056632</ref>
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides. [[http://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN]] PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.<ref>PMID:21076856</ref>

	==About this Structure==		==About this Structure==
Line 11:		Line 13:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:018375760</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018375760</ref><references group="xtra"/><references/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Pioszak, A A.]]		[[Category: Pioszak, A A.]]

OCA: Protected "3c4m" [edit=sysop:move=sysop]

2013-01-07T23:14:39Z

Protected "3c4m" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:14, 7 January 2013

OCA at 23:14, 7 January 2013

2013-01-07T23:14:36Z

←Older revision		Revision as of 23:14, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3c4m.png\|left\|200px]]		[[Image:3c4m.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3c4m", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3c4m\| PDB=3c4m \| SCENE= }}		{{STRUCTURE_3c4m\| PDB=3c4m \| SCENE= }}

	===Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)===		===Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18375760}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18375760 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18375760}}		{{ABSTRACT_PUBMED_18375760}}

	==About this Structure==		==About this Structure==
-	~~3C4M~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli~~,_homo_sapiens~~ Escherichia coli~~, homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA].	+	[[3c4m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18375760~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018375760</ref><references group="xtra"/>
-	[[Category: Escherichia coli~~, homo sapiens~~]]	+	[[Category: Escherichia coli]]
	[[Category: Pioszak, A A.]]		[[Category: Pioszak, A A.]]
	[[Category: Xu, H E.]]		[[Category: Xu, H E.]]
Line 35:		Line 23:
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Parathyroid hormone]]		[[Category: Parathyroid hormone]]
-	[[Category: Periplasm]]
	[[Category: Receptor]]		[[Category: Receptor]]
	[[Category: Secreted]]		[[Category: Secreted]]
-	[[Category: Signal]]
	[[Category: Sugar transport]]		[[Category: Sugar transport]]
	[[Category: Transducer]]		[[Category: Transducer]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]
	[[Category: Transport]]		[[Category: Transport]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 03:40:44 2009''

OCA at 01:40, 18 February 2009

2009-02-18T01:40:45Z

←Older revision		Revision as of 01:40, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3C4M is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli,_homo_sapiens Escherichia coli, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA].	+	3C4M is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli,_homo_sapiens Escherichia coli, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4M OCA].

	==Reference==		==Reference==
-	~~Molecular recognition of parathyroid hormone by its G protein-coupled receptor., Pioszak AA, Xu HE, Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5034-9. Epub 2008 Mar 28.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18375760 18375760]~~	+	<ref group="xtra">PMID:18375760</ref><references group="xtra"/>
	[[Category: Escherichia coli, homo sapiens]]		[[Category: Escherichia coli, homo sapiens]]
-	[[Category: Protein complex]]
	[[Category: Pioszak, A A.]]		[[Category: Pioszak, A A.]]
	[[Category: Xu, H E.]]		[[Category: Xu, H E.]]
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	[[Category: Transport]]		[[Category: Transport]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 09~~:55:~~19 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 03:40:44 2009''

OCA at 06:55, 29 July 2008

2008-07-29T06:55:22Z

←Older revision		Revision as of 06:55, 29 July 2008
Line 1:		Line 1:
-	[[Image:3c4m.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3c4m.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_3c4m\| PDB=3c4m \| SCENE= }}		{{STRUCTURE_3c4m\| PDB=3c4m \| SCENE= }}

-	~~'''~~Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)~~'''~~	+	===Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)===


-	~~==Overview==~~	+	<!--
-	~~Parathyroid hormone (PTH) is central to calcium homeostasis and bone maintenance in vertebrates~~, ~~and as such it has been used for treating osteoporosis. It acts primarily by binding to its receptor~~, ~~PTH1R, a member of~~ the ~~class B G protein-coupled receptor~~ (~~GPCR) family that also includes receptors for glucagon, calcitonin, and other therapeutically important peptide hormones~~. Despite considerable interest and much research, determining the structure of the receptor-hormone complex has been hindered by difficulties in purifying the receptor and obtaining diffraction-quality crystals. ~~Here~~, ~~we present a method for expression and purification of~~ the ~~extracellular domain (ECD) of human PTH1R engineered as a maltose-binding protein (MBP) fusion that readily crystallizes~~. ~~The 1.95~~-~~A structure of PTH bound to the MBP~~-PTH1R-ECD fusion reveals that PTH docks as an amphipathic helix into a central hydrophobic groove formed by a three-layer alpha-beta-betaalpha fold of the PTH1R ECD, resembling a hot dog in a bun. Conservation in the ECD scaffold and the helical structure of peptide hormones emphasizes this hot dog model as a general mechanism of hormone recognition common to class B GPCRs. Our findings reveal critical insights into PTH actions and provide a rational template for drug design that targets this hormone signaling pathway.	+	The line below this paragraph, {{ABSTRACT_PUBMED_18375760}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18375760 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18375760}}

	==About this Structure==		==About this Structure==
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	[[Category: Transmembrane]]		[[Category: Transmembrane]]
	[[Category: Transport]]		[[Category: Transport]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:55:19 2008''