3c75 - Revision history

OCA at 10:24, 12 August 2020

2020-08-12T10:24:17Z

←Older revision		Revision as of 10:24, 12 August 2020
Line 1:		Line 1:

	==Paracoccus versutus methylamine dehydrogenase in complex with amicyanin==		==Paracoccus versutus methylamine dehydrogenase in complex with amicyanin==
-	<StructureSection load='3c75' size='340' side='right' caption='[[3c75]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3c75' size='340' side='right'caption='[[3c75]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3c75]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3C75 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3c75]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3C75 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylamine_dehydrogenase_(amicyanin) Methylamine dehydrogenase (amicyanin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.9.1 1.4.9.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylamine_dehydrogenase_(amicyanin) Methylamine dehydrogenase (amicyanin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.9.1 1.4.9.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3c75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c75 OCA], [http://pdbe.org/3c75 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c75 RCSB], [http://www.ebi.ac.uk/pdbsum/3c75 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c75 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3c75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c75 OCA], [http://pdbe.org/3c75 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c75 RCSB], [http://www.ebi.ac.uk/pdbsum/3c75 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c75 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 37:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Paracoccus versutus]]		[[Category: Paracoccus versutus]]
	[[Category: Biermann, N]]		[[Category: Biermann, N]]

OCA at 13:40, 7 November 2018

2018-11-07T13:40:44Z

←Older revision		Revision as of 13:40, 7 November 2018
Line 1:		Line 1:
		+
	==Paracoccus versutus methylamine dehydrogenase in complex with amicyanin==		==Paracoccus versutus methylamine dehydrogenase in complex with amicyanin==
	<StructureSection load='3c75' size='340' side='right' caption='[[3c75]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3c75' size='340' side='right' caption='[[3c75]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylamine_dehydrogenase_(amicyanin) Methylamine dehydrogenase (amicyanin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.9.1 1.4.9.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylamine_dehydrogenase_(amicyanin) Methylamine dehydrogenase (amicyanin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.9.1 1.4.9.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c75 OCA], [http://pdbe.org/3c75 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c75 RCSB], [http://www.ebi.ac.uk/pdbsum/3c75 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c75 OCA], [http://pdbe.org/3c75 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c75 RCSB], [http://www.ebi.ac.uk/pdbsum/3c75 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c75 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/3c75_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/3c75_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 50:		Line 51:
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
		+	[[Category: Periplasm]]
	[[Category: Pyrrolidone carboxylic acid]]		[[Category: Pyrrolidone carboxylic acid]]
	[[Category: Transport]]		[[Category: Transport]]
	[[Category: Ttq]]		[[Category: Ttq]]

OCA at 04:26, 8 February 2016

2016-02-08T04:26:13Z

←Older revision		Revision as of 04:26, 8 February 2016
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Amine_dehydrogenase Amine~~ dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.3 1.4.99.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylamine_dehydrogenase_(amicyanin) Methylamine dehydrogenase (amicyanin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.9.1 1.4.9.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c75 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c75 RCSB], [http://www.ebi.ac.uk/pdbsum/3c75 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c75 OCA], [http://pdbe.org/3c75 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c75 RCSB], [http://www.ebi.ac.uk/pdbsum/3c75 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c75 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3c75" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Amine dehydrogenase]]
	[[Category: Paracoccus versutus]]		[[Category: Paracoccus versutus]]
	[[Category: Biermann, N]]		[[Category: Biermann, N]]

OCA at 20:36, 24 December 2014

2014-12-24T20:36:30Z

←Older revision		Revision as of 20:36, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3c75]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C75 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3c75]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C75 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amine_dehydrogenase Amine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.3 1.4.99.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amine_dehydrogenase Amine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.3 1.4.99.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c75 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c75 RCSB], [http://www.ebi.ac.uk/pdbsum/3c75 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c75 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c75 RCSB], [http://www.ebi.ac.uk/pdbsum/3c75 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DHMH_PARVE DHMH_PARVE]] Methylamine dehydrogenase carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin. [[http://www.uniprot.org/uniprot/AMCY_PARVE AMCY_PARVE]] Primary acceptor of electrons from methylamine dehydrogenase. Passes those electrons on either a soluble cytochrome c or to pseudoazurin. [[http://www.uniprot.org/uniprot/DHML_PARVE DHML_PARVE]] Methylamine dehydrogenase carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Amine dehydrogenase]]		[[Category: Amine dehydrogenase]]
	[[Category: Paracoccus versutus]]		[[Category: Paracoccus versutus]]
-	[[Category: Biermann, N.]]	+	[[Category: Biermann, N]]
-	[[Category: Cavalieri, C.]]	+	[[Category: Cavalieri, C]]
-	[[Category: Einsle, O.]]	+	[[Category: Einsle, O]]
-	[[Category: Ferrari, D.]]	+	[[Category: Ferrari, D]]
-	[[Category: Merli, A.]]	+	[[Category: Merli, A]]
-	[[Category: Rossi, G L.]]	+	[[Category: Rossi, G L]]
-	[[Category: Ubbink, M.]]	+	[[Category: Ubbink, M]]
-	[[Category: Vlasie, M D.]]	+	[[Category: Vlasie, M D]]
	[[Category: Copper protein]]		[[Category: Copper protein]]
	[[Category: Electron transfer complex]]		[[Category: Electron transfer complex]]

OCA at 07:49, 29 September 2014

2014-09-29T07:49:57Z

←Older revision		Revision as of 07:49, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3c75.~~png~~\|~~left~~\|200px]]	+	==Paracoccus versutus methylamine dehydrogenase in complex with amicyanin==
		+	<StructureSection load='3c75' size='340' side='right' caption='[[3c75]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3c75]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C75 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amine_dehydrogenase Amine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.3 1.4.99.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c75 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c75 RCSB], [http://www.ebi.ac.uk/pdbsum/3c75 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/3c75_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Methylamine can be used as the sole carbon source of certain methylotrophic bacteria. Methylamine dehydrogenase catalyzes the conversion of methylamine into formaldehyde and donates electrons to the electron transfer protein amicyanin. The crystal structure of the complex of methylamine dehydrogenase and amicyanin from Paracoccus versutus has been determined, and the rate of electron transfer from the tryptophan tryptophylquinone cofactor of methylamine dehydrogenase to the copper ion of amicyanin in solution has been determined. In the presence of monovalent ions, the rate of electron transfer from the methylamine-reduced TTQ is much higher than in their absence. In general, the kinetics are similar to those observed for the system from Paracoccus denitrificans. The complex in solution has been studied using nuclear magnetic resonance. Signals of perdeuterated, (15)N-enriched amicyanin bound to methylamine dehydrogenase are observed. Chemical shift perturbation analysis indicates that the dissociation rate constant is approximately 250 s (-1) and that amicyanin assumes a well-defined position in the complex in solution. The most affected residues are in the interface observed in the crystal structure, whereas smaller chemical shift changes extend to deep inside the protein. These perturbations can be correlated to small differences in the hydrogen bond network observed in the crystal structures of free and bound amicyanin. This study indicates that chemical shift changes can be used as reliable indicators of subtle structural changes even in a complex larger than 100 kDa.

-	~~{{STRUCTURE_3c75\| PDB=3c75 \| SCENE= }}~~	+	Structural Comparison of Crystal and Solution States of the 138 kDa Complex of Methylamine Dehydrogenase and Amicyanin from Paracoccus versutus.,Cavalieri C, Biermann N, Vlasie MD, Einsle O, Merli A, Ferrari D, Rossi GL, Ubbink M Biochemistry. 2008 May 31. PMID:18512962<ref>PMID:18512962</ref>

-	~~===Paracoccus versutus methylamine dehydrogenase in complex with amicyanin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18512962}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3c75]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Paracoccus_versutus Paracoccus versutus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA].~~	+

	==See Also==		==See Also==
	*[[Methylamine dehydrogenase\|Methylamine dehydrogenase]]		*[[Methylamine dehydrogenase\|Methylamine dehydrogenase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018512962<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Amine dehydrogenase]]		[[Category: Amine dehydrogenase]]
	[[Category: Paracoccus versutus]]		[[Category: Paracoccus versutus]]

OCA at 08:54, 20 October 2012

2012-10-20T08:54:18Z

←Older revision		Revision as of 08:54, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3c75]] is a 6 chain structure ~~of [[Methylamine dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA].	+	[[3c75]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA].

	==See Also==		==See Also==

OCA: Protected "3c75" [edit=sysop:move=sysop]

2012-07-25T21:09:02Z

Protected "3c75" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:09, 25 July 2012

OCA at 21:09, 25 July 2012

2012-07-25T21:09:00Z

←Older revision		Revision as of 21:09, 25 July 2012
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-	{{Seed}}
	[[Image:3c75.png\|left\|200px]]		[[Image:3c75.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3c75", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3c75\| PDB=3c75 \| SCENE= }}		{{STRUCTURE_3c75\| PDB=3c75 \| SCENE= }}

	===Paracoccus versutus methylamine dehydrogenase in complex with amicyanin===		===Paracoccus versutus methylamine dehydrogenase in complex with amicyanin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18512962}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18512962 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18512962}}		{{ABSTRACT_PUBMED_18512962}}

	==About this Structure==		==About this Structure==
-	~~3C75~~ is a 6 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA].	+	[[3c75]] is a 6 chain structure of [[Methylamine dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA].
		+
		+	==See Also==
		+	*[[Methylamine dehydrogenase\|Methylamine dehydrogenase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18512962~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018512962</ref><references group="xtra"/>
	[[Category: Amine dehydrogenase]]		[[Category: Amine dehydrogenase]]
	[[Category: Paracoccus versutus]]		[[Category: Paracoccus versutus]]
Line 39:		Line 30:
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-	[[Category: Periplasm]]
	[[Category: Pyrrolidone carboxylic acid]]		[[Category: Pyrrolidone carboxylic acid]]
	[[Category: Transport]]		[[Category: Transport]]
	[[Category: Ttq]]		[[Category: Ttq]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 14 08:59:20 2010''

OCA at 05:59, 14 April 2010

2010-04-14T05:59:21Z

←Older revision		Revision as of 05:59, 14 April 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3c75.~~jpg~~\|left\|200px]]	+	[[Image:3c75.png\|left\|200px]]

	<!--		<!--
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3C75 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA].	+	3C75 is a 6 chains structure with sequences from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA].

	==Reference==		==Reference==
-	Structural Comparison of Crystal and Solution States of the 138 kDa Complex of Methylamine Dehydrogenase and Amicyanin from Paracoccus versutus., Cavalieri C, Biermann N, Vlasie MD, Einsle O, Merli A, Ferrari D, Rossi GL, Ubbink M, Biochemistry. 2008 May 31. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18512962 18512962]~~	+	<ref group="xtra">PMID:18512962</ref><references group="xtra"/>
	[[Category: Amine dehydrogenase]]		[[Category: Amine dehydrogenase]]
	[[Category: Paracoccus versutus]]		[[Category: Paracoccus versutus]]
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	[[Category: Ttq]]		[[Category: Ttq]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Dec 31 10~~:17:~~06 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 14 08:59:20 2010''

OCA at 08:17, 31 December 2008

2008-12-31T08:17:12Z

←Older revision		Revision as of 08:17, 31 December 2008
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3c75.jpg\|left\|200px]]

-	The ~~entry 3c75~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3c75", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3c75\| PDB=3c75 \| SCENE= }}

-	~~Authors: Cavalieri, C., Biermann, N., Vlasie, M.D., Einsle, O., Merli, A., Ferrari, D., Rossi, G.L., Ubbink, M.~~	+	===Paracoccus versutus methylamine dehydrogenase in complex with amicyanin===

-	Description: Paracoccus versutus methylamine dehydrogenase in complex with amicyanin

		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18512962}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18512962 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18512962}}

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jun 11 09~~:30:10 2008''	+	==About this Structure==
		+	3C75 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C75 OCA].
		+
		+	==Reference==
		+	Structural Comparison of Crystal and Solution States of the 138 kDa Complex of Methylamine Dehydrogenase and Amicyanin from Paracoccus versutus., Cavalieri C, Biermann N, Vlasie MD, Einsle O, Merli A, Ferrari D, Rossi GL, Ubbink M, Biochemistry. 2008 May 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18512962 18512962]
		+	[[Category: Amine dehydrogenase]]
		+	[[Category: Paracoccus versutus]]
		+	[[Category: Biermann, N.]]
		+	[[Category: Cavalieri, C.]]
		+	[[Category: Einsle, O.]]
		+	[[Category: Ferrari, D.]]
		+	[[Category: Merli, A.]]
		+	[[Category: Rossi, G L.]]
		+	[[Category: Ubbink, M.]]
		+	[[Category: Vlasie, M D.]]
		+	[[Category: Copper protein]]
		+	[[Category: Electron transfer complex]]
		+	[[Category: Electron transport]]
		+	[[Category: Metal-binding]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Periplasm]]
		+	[[Category: Pyrrolidone carboxylic acid]]
		+	[[Category: Transport]]
		+	[[Category: Ttq]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 31 10:17:06 2008''