3cfi - Revision history

OCA at 07:53, 27 January 2022

2022-01-27T07:53:59Z

←Older revision		Revision as of 07:53, 27 January 2022
Line 1:		Line 1:

	==Nanobody-aided structure determination of the EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus==		==Nanobody-aided structure determination of the EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus==
-	<StructureSection load='3cfi' size='340' side='right' caption='[[3cfi]], [[Resolution\|resolution]] 2.58Å' scene=''>	+	<StructureSection load='3cfi' size='340' side='right'caption='[[3cfi]], [[Resolution\|resolution]] 2.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cfi]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_27562 Atcc 27562] and [~~http~~://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cfi]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_27562 Atcc 27562] and [https://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CFI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EpsI ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), EpsJ ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), NBEPSIJ_11 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EpsI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), EpsJ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), NBEPSIJ_11 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfi OCA], [~~http~~://pdbe.org/3cfi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3cfi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3cfi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3cfi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfi OCA], [https://pdbe.org/3cfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cfi RCSB], [https://www.ebi.ac.uk/pdbsum/3cfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cfi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 36:		Line 36:
	[[Category: Atcc 27562]]		[[Category: Atcc 27562]]
	[[Category: Camelus glama]]		[[Category: Camelus glama]]
		+	[[Category: Large Structures]]
	[[Category: Hol, W G.J]]		[[Category: Hol, W G.J]]
	[[Category: Korotkov, K V]]		[[Category: Korotkov, K V]]

OCA at 13:13, 7 November 2018

2018-11-07T13:13:01Z

←Older revision		Revision as of 13:13, 7 November 2018
Line 1:		Line 1:
		+
	==Nanobody-aided structure determination of the EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus==		==Nanobody-aided structure determination of the EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus==
	<StructureSection load='3cfi' size='340' side='right' caption='[[3cfi]], [[Resolution\|resolution]] 2.58Å' scene=''>		<StructureSection load='3cfi' size='340' side='right' caption='[[3cfi]], [[Resolution\|resolution]] 2.58Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EpsI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), EpsJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), NBEPSIJ_11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EpsI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), EpsJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), NBEPSIJ_11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfi OCA], [http://pdbe.org/3cfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cfi RCSB], [http://www.ebi.ac.uk/pdbsum/3cfi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfi OCA], [http://pdbe.org/3cfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cfi RCSB], [http://www.ebi.ac.uk/pdbsum/3cfi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cfi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/3cfi_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/3cfi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:55, 9 February 2016

2016-02-09T08:55:25Z

←Older revision		Revision as of 08:55, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3cfi' size='340' side='right' caption='[[3cfi]], [[Resolution\|resolution]] 2.58Å' scene=''>		<StructureSection load='3cfi' size='340' side='right' caption='[[3cfi]], [[Resolution\|resolution]] 2.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cfi]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lama_glama Lama glama~~] and [http://en.wikipedia.org/wiki/~~Vibrio_vulnificus Vibrio vulnificus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cfi]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27562 Atcc 27562] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CFI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EpsI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ~~Vibrio vulnificus~~]), EpsJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ~~Vibrio vulnificus~~]), NBEPSIJ_11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 ~~Lama~~ glama])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EpsI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), EpsJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 ATCC 27562]), NBEPSIJ_11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cfi RCSB], [http://www.ebi.ac.uk/pdbsum/3cfi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfi OCA], [http://pdbe.org/3cfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cfi RCSB], [http://www.ebi.ac.uk/pdbsum/3cfi PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cfi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3cfi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lama glama~~]]	+	[[Category: Atcc 27562]]
-	[[Category: ~~Vibrio vulnificus~~]]	+	[[Category: Camelus glama]]
	[[Category: Hol, W G.J]]		[[Category: Hol, W G.J]]
	[[Category: Korotkov, K V]]		[[Category: Korotkov, K V]]

OCA at 09:57, 20 January 2015

2015-01-20T09:57:40Z

←Older revision		Revision as of 09:57, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3cfi]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CFI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3cfi]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CFI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EpsI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 Vibrio vulnificus]), EpsJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 Vibrio vulnificus]), NBEPSIJ_11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Lama glama])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EpsI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 Vibrio vulnificus]), EpsJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 Vibrio vulnificus]), NBEPSIJ_11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Lama glama])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cfi RCSB], [http://www.ebi.ac.uk/pdbsum/3cfi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cfi RCSB], [http://www.ebi.ac.uk/pdbsum/3cfi PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Lama glama]]		[[Category: Lama glama]]
	[[Category: Vibrio vulnificus]]		[[Category: Vibrio vulnificus]]
-	[[Category: Hol, W G.J.]]	+	[[Category: Hol, W G.J]]
-	[[Category: Korotkov, K V.]]	+	[[Category: Korotkov, K V]]
-	[[Category: Lam, A Y.]]	+	[[Category: Lam, A Y]]
-	[[Category: Pardon, E.]]	+	[[Category: Pardon, E]]
-	[[Category: Steyaert, J.]]	+	[[Category: Steyaert, J]]
	[[Category: General secretory pathway]]		[[Category: General secretory pathway]]
	[[Category: Methylation]]		[[Category: Methylation]]

OCA at 08:20, 29 September 2014

2014-09-29T08:20:43Z

←Older revision		Revision as of 08:20, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3cfi.~~png~~\|~~left~~\|200px]]	+	==Nanobody-aided structure determination of the EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus==
		+	<StructureSection load='3cfi' size='340' side='right' caption='[[3cfi]], [[Resolution\|resolution]] 2.58Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3cfi]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CFI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EpsI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 Vibrio vulnificus]), EpsJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=672 Vibrio vulnificus]), NBEPSIJ_11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Lama glama])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cfi RCSB], [http://www.ebi.ac.uk/pdbsum/3cfi PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/3cfi_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pseudopilins form the central pseudopilus of the sophisticated bacterial type 2 secretion systems. The crystallization of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus was greatly accelerated by the use of nanobodies, which are the smallest antigen-binding fragments derived from heavy-chain only camelid antibodies. Seven anti-EpsI:EpsJ nanobodies were generated and co-crystallization of EpsI:EpsJ nanobody complexes yielded several crystal forms very rapidly. In the structure solved, the nanobodies are arranged in planes throughout the crystal lattice, linking layers of EpsI:EpsJ heterodimers. The EpsI:EpsJ dimer observed confirms a right-handed architecture of the pseudopilus, but, compared to a previous structure of the EpsI:EpsJ heterodimer, EpsI differs 6 degrees in orientation with respect to EpsJ; one loop of EpsJ is shifted by approximately 5A due to interactions with the nanobody; and a second loop of EpsJ underwent a major change of 17A without contacts with the nanobody. Clearly, nanobodies accelerate dramatically the crystallization of recalcitrant protein complexes and can reveal conformational flexibility not observed before.

-	~~{{STRUCTURE_3cfi\| PDB=3cfi \| SCENE= }}~~	+	Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus.,Lam AY, Pardon E, Korotkov KV, Hol WG, Steyaert J J Struct Biol. 2009 Apr;166(1):8-15. Epub 2008 Dec 10. PMID:19118632<ref>PMID:19118632</ref>

-	~~===Nanobody-aided structure determination~~ of the ~~EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19118632}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3cfi]] is a 12 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lama_glama Lama glama] and [http://en~~.~~wikipedia.org~~/~~wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA].~~	+

	==See Also==		==See Also==
	*[[Pseudopilin\|Pseudopilin]]		*[[Pseudopilin\|Pseudopilin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019118632<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Lama glama]]		[[Category: Lama glama]]
	[[Category: Vibrio vulnificus]]		[[Category: Vibrio vulnificus]]

OCA at 13:20, 20 October 2012

2012-10-20T13:20:22Z

←Older revision		Revision as of 13:20, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3cfi]] is a 12 chain structure ~~of [[Pseudopilin]]~~ with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA].	+	[[3cfi]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA].

	==See Also==		==See Also==

OCA: Protected "3cfi" [edit=sysop:move=sysop]

2012-07-26T11:38:50Z

Protected "3cfi" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:38, 26 July 2012

OCA at 11:38, 26 July 2012

2012-07-26T11:38:48Z

←Older revision		Revision as of 11:38, 26 July 2012
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-	{{Seed}}
	[[Image:3cfi.png\|left\|200px]]		[[Image:3cfi.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3cfi", creates the "Structure Box" on the page.
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_3cfi\| PDB=3cfi \| SCENE= }}		{{STRUCTURE_3cfi\| PDB=3cfi \| SCENE= }}

	===Nanobody-aided structure determination of the EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus===		===Nanobody-aided structure determination of the EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19118632}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_19118632}}		{{ABSTRACT_PUBMED_19118632}}

	==About this Structure==		==About this Structure==
-	~~3CFI~~ is a 12 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA].	+	[[3cfi]] is a 12 chain structure of [[Pseudopilin]] with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA].
		+
		+	==See Also==
		+	*[[Pseudopilin\|Pseudopilin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19118632~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019118632</ref><references group="xtra"/>
	[[Category: Lama glama]]		[[Category: Lama glama]]
	[[Category: Vibrio vulnificus]]		[[Category: Vibrio vulnificus]]
Line 33:		Line 24:
	[[Category: General secretory pathway]]		[[Category: General secretory pathway]]
	[[Category: Methylation]]		[[Category: Methylation]]
-	[[Category: Protein transport/immune system complex]]	+	[[Category: Protein transport-immune system complex]]
	[[Category: Pseudopilin]]		[[Category: Pseudopilin]]
	[[Category: Single chain antibody]]		[[Category: Single chain antibody]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 2 16:21:43 2009''

OCA at 13:21, 2 April 2009

2009-04-02T13:21:43Z

←Older revision		Revision as of 13:21, 2 April 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3CFI is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA].	+	3CFI is a 12 chains structure of sequences from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA].

	==Reference==		==Reference==
-	~~Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus., Lam AY, Pardon E, Korotkov KV, Hol WG, Steyaert J, J Struct Biol. 2008 Dec 10.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/19118632 19118632]~~	+	<ref group="xtra">PMID:19118632</ref><references group="xtra"/>
	[[Category: Lama glama]]		[[Category: Lama glama]]
-	[[Category: Protein complex]]
	[[Category: Vibrio vulnificus]]		[[Category: Vibrio vulnificus]]
-	[[Category: ~~Pdbx_ordinal=~~, ~~<PDBx~~:~~audit_author~~.]]	+	[[Category: Hol, W G.J.]]
		+	[[Category: Korotkov, K V.]]
		+	[[Category: Lam, A Y.]]
		+	[[Category: Pardon, E.]]
		+	[[Category: Steyaert, J.]]
	[[Category: General secretory pathway]]		[[Category: General secretory pathway]]
	[[Category: Methylation]]		[[Category: Methylation]]
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	[[Category: Single chain antibody]]		[[Category: Single chain antibody]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Feb 25 20~~:13:41 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 2 16:21:43 2009''

OCA at 18:14, 25 February 2009

2009-02-25T18:14:08Z

←Older revision		Revision as of 18:14, 25 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3CFI is a ~~12 chains~~ structure of sequences from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA].	+	3CFI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] and [http://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFI OCA].

	==Reference==		==Reference==
-	~~<ref group="xtra">~~PMID:~~19118632<~~/~~ref><references group="xtra"~~/>	+	Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus., Lam AY, Pardon E, Korotkov KV, Hol WG, Steyaert J, J Struct Biol. 2008 Dec 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19118632 19118632]
	[[Category: Lama glama]]		[[Category: Lama glama]]
		+	[[Category: Protein complex]]
	[[Category: Vibrio vulnificus]]		[[Category: Vibrio vulnificus]]
-	[[Category: ~~Hol~~, ~~W G.J.]]~~	+	[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
-	~~[[Category~~: ~~Korotkov, K V.]]~~	+
-	~~[[Category: Lam, A Y.]]~~	+
-	~~[[Category: Pardon, E.]]~~	+
-	~~[[Category: Steyaert, J~~.]]	+
	[[Category: General secretory pathway]]		[[Category: General secretory pathway]]
	[[Category: Methylation]]		[[Category: Methylation]]
Line 37:		Line 34:
	[[Category: Single chain antibody]]		[[Category: Single chain antibody]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 25 08:16:58 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 25 20:13:41 2009''