3chf - Revision history

OCA at 12:28, 30 August 2023

2023-08-30T12:28:38Z

←Older revision		Revision as of 12:28, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='3chf' size='340' side='right'caption='[[3chf]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3chf' size='340' side='right'caption='[[3chf]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3chf]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3CHF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3chf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CHF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene~~></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [https://pdbe.org/3chf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [https://www.ebi.ac.uk/pdbsum/3chf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3chf ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [~~http~~://pdbe.org/3chf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3chf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3chf ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CHIB1_ASPFM CHIB1_ASPFM]] Major secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Plays a role in the morphogenesis and autolysis (By similarity).	+	[https://www.uniprot.org/uniprot/CHIB1_ASPFM CHIB1_ASPFM] Major secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Plays a role in the morphogenesis and autolysis (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chitinase~~]]	+	[[Category: Aspergillus fumigatus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Aalten, D M.F van]]~~	+	[[Category: Andersen OA]]
-	[[Category: Andersen~~, O A~~]]	+	[[Category: Van Aalten DMF]]
-	[[Category: ~~Glycosidase]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Peptide inhibitor~~]]	+

OCA at 10:27, 12 August 2020

2020-08-12T10:27:27Z

←Older revision		Revision as of 10:27, 12 August 2020
Line 1:		Line 1:

	==Crystal structure of Aspergillus fumigatus chitinase B1 in complex with tetrapeptide==		==Crystal structure of Aspergillus fumigatus chitinase B1 in complex with tetrapeptide==
-	<StructureSection load='3chf' size='340' side='right' caption='[[3chf]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='3chf' size='340' side='right'caption='[[3chf]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3chf]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CHF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3chf]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3CHF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [http://pdbe.org/3chf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [http://www.ebi.ac.uk/pdbsum/3chf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3chf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [http://pdbe.org/3chf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [http://www.ebi.ac.uk/pdbsum/3chf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3chf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Chitinase\|Chitinase]]	+	*[[Chitinase 3D structures\|Chitinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Chitinase]]		[[Category: Chitinase]]
		+	[[Category: Large Structures]]
	[[Category: Aalten, D M.F van]]		[[Category: Aalten, D M.F van]]
	[[Category: Andersen, O A]]		[[Category: Andersen, O A]]

OCA at 14:03, 7 November 2018

2018-11-07T14:03:27Z

←Older revision		Revision as of 14:03, 7 November 2018
Line 1:		Line 1:
		+
	==Crystal structure of Aspergillus fumigatus chitinase B1 in complex with tetrapeptide==		==Crystal structure of Aspergillus fumigatus chitinase B1 in complex with tetrapeptide==
	<StructureSection load='3chf' size='340' side='right' caption='[[3chf]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3chf' size='340' side='right' caption='[[3chf]], [[Resolution\|resolution]] 1.95Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [http://pdbe.org/3chf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [http://www.ebi.ac.uk/pdbsum/3chf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [http://pdbe.org/3chf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [http://www.ebi.ac.uk/pdbsum/3chf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3chf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/3chf_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/3chf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:48, 7 February 2016

2016-02-07T23:48:56Z

←Older revision		Revision as of 23:48, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3chf' size='340' side='right' caption='[[3chf]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3chf' size='340' side='right' caption='[[3chf]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3chf]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CHF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3chf]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CHF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chiB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 Aspergillus fumigatus])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [http://www.ebi.ac.uk/pdbsum/3chf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [http://pdbe.org/3chf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [http://www.ebi.ac.uk/pdbsum/3chf PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHIB1_ASPFM CHIB1_ASPFM]] Major secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Plays a role in the morphogenesis and autolysis (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3chf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3chf" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aspergillus fumigatus]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
	[[Category: Aalten, D M.F van]]		[[Category: Aalten, D M.F van]]

OCA at 10:54, 20 January 2015

2015-01-20T10:54:52Z

←Older revision		Revision as of 10:54, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3chf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CHF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3chf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CHF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chiB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 Aspergillus fumigatus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chiB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 Aspergillus fumigatus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [http://www.ebi.ac.uk/pdbsum/3chf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [http://www.ebi.ac.uk/pdbsum/3chf PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 37:
	[[Category: Aspergillus fumigatus]]		[[Category: Aspergillus fumigatus]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
-	[[Category: Aalten, D M.F van.]]	+	[[Category: Aalten, D M.F van]]
-	[[Category: Andersen, O A~~.]]~~	+	[[Category: Andersen, O A]]
-	~~[[Category: Chitinase~~]]	+
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Peptide inhibitor]]		[[Category: Peptide inhibitor]]

OCA at 09:19, 29 September 2014

2014-09-29T09:19:57Z

←Older revision		Revision as of 09:19, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3chf.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Aspergillus fumigatus chitinase B1 in complex with tetrapeptide==
		+	<StructureSection load='3chf' size='340' side='right' caption='[[3chf]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3chf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CHF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1w9v\|1w9v]], [[1w9p\|1w9p]], [[3ch9\|3ch9]], [[3chc\|3chc]], [[3chd\|3chd]], [[3che\|3che]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chiB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 Aspergillus fumigatus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3chf RCSB], [http://www.ebi.ac.uk/pdbsum/3chf PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/3chf_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chitinase inhibitors have chemotherapeutic potential as fungicides, pesticides, and antiasthmatics. Argifin, a natural product cyclopentapeptide, competitively inhibits family 18 chitinases in the nanomolar to micromolar range and shows extensive substrate mimicry. In an attempt to map the active fragments of this large natural product, the cyclopentapeptide was progressively dissected down to four linear peptides and dimethylguanylurea, synthesized using a combination of solution and solid phase peptide synthesis. The peptide fragments inhibit chitinase B1 from Aspergillus fumigatus (AfChiB1), the human chitotriosidase, and chitinase activity in lung homogenates from a murine model of chronic asthma, with potencies ranging from high nanomolar to high micromolar inhibition. X-ray crystallographic analysis of the chitinase-inhibitor complexes revealed that the conformations of the linear peptides were remarkably similar to that of the natural product. Strikingly, the dimethylguanylurea fragment, representing only a quarter of the natural product mass, was found to harbor all significant interactions with the protein and binds with unusually high efficiency. The data provide useful information that could lead to the generation of drug-like, natural product-based chitinase inhibitors.

-	~~{{STRUCTURE_3chf\| PDB=3chf \| SCENE= }}~~	+	Structure-based dissection of the natural product cyclopentapeptide chitinase inhibitor argifin.,Andersen OA, Nathubhai A, Dixon MJ, Eggleston IM, van Aalten DM Chem Biol. 2008 Mar;15(3):295-301. PMID:18355729<ref>PMID:18355729</ref>

-	~~===Crystal structure~~ of ~~Aspergillus fumigatus chitinase B1 in complex with tetrapeptide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18355729}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3chf]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA].~~	+

	==See Also==		==See Also==
	*[[Chitinase\|Chitinase]]		*[[Chitinase\|Chitinase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018355729<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Aspergillus fumigatus]]		[[Category: Aspergillus fumigatus]]
	[[Category: Chitinase]]		[[Category: Chitinase]]

OCA at 08:12, 20 October 2012

2012-10-20T08:12:22Z

←Older revision		Revision as of 08:12, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3chf]] is a 4 chain structure ~~of [[Chitinase]]~~ with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA].	+	[[3chf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA].

	==See Also==		==See Also==

OCA: Protected "3chf" [edit=sysop:move=sysop]

2012-07-25T18:47:27Z

Protected "3chf" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:47, 25 July 2012

OCA at 18:47, 25 July 2012

2012-07-25T18:47:26Z

←Older revision		Revision as of 18:47, 25 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3chf.png\|left\|200px]]		[[Image:3chf.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3chf", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3chf\| PDB=3chf \| SCENE= }}		{{STRUCTURE_3chf\| PDB=3chf \| SCENE= }}

	===Crystal structure of Aspergillus fumigatus chitinase B1 in complex with tetrapeptide===		===Crystal structure of Aspergillus fumigatus chitinase B1 in complex with tetrapeptide===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18355729}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18355729 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18355729}}		{{ABSTRACT_PUBMED_18355729}}

	==About this Structure==		==About this Structure==
-	~~3CHF~~ is a ~~2 chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA].	+	[[3chf]] is a 4 chain structure of [[Chitinase]] with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA].
		+
		+	==See Also==
		+	*[[Chitinase\|Chitinase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18355729~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018355729</ref><references group="xtra"/>
	[[Category: Aspergillus fumigatus]]		[[Category: Aspergillus fumigatus]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
Line 30:		Line 21:
	[[Category: Chitinase]]		[[Category: Chitinase]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
-	[[Category: Hydrolase]]	+	[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Peptide inhibitor]]		[[Category: Peptide inhibitor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:17:54 2009''

OCA at 15:17, 16 February 2009

2009-02-16T15:17:57Z

←Older revision		Revision as of 15:17, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3CHF is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA].	+	3CHF is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHF OCA].

	==Reference==		==Reference==
-	~~Structure-based dissection of the natural product cyclopentapeptide chitinase inhibitor argifin., Andersen OA, Nathubhai A, Dixon MJ, Eggleston IM, van Aalten DM, Chem Biol. 2008 Mar;15(3):295-301.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18355729 18355729]~~	+	<ref group="xtra">PMID:18355729</ref><references group="xtra"/>
	[[Category: Aspergillus fumigatus]]		[[Category: Aspergillus fumigatus]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
-	[[Category: Single protein]]
	[[Category: Aalten, D M.F van.]]		[[Category: Aalten, D M.F van.]]
	[[Category: Andersen, O A.]]		[[Category: Andersen, O A.]]
Line 34:		Line 33:
	[[Category: Peptide inhibitor]]		[[Category: Peptide inhibitor]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 20~~:39:~~32 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:17:54 2009''