3cla - Revision history

OCA at 09:36, 21 February 2024

2024-02-21T09:36:23Z

←Older revision		Revision as of 09:36, 21 February 2024
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	<StructureSection load='3cla' size='340' side='right'caption='[[3cla]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3cla' size='340' side='right'caption='[[3cla]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cla]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3CLA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cla]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>~~, ~~<scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Chloramphenicol_O-acetyltransferase Chloramphenicol O-acetyltransferase]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.28 2.3.1.28]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3cla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cla OCA], [~~http~~://pdbe.org/3cla PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3cla RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3cla PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3cla ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cla OCA], [https://pdbe.org/3cla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cla RCSB], [https://www.ebi.ac.uk/pdbsum/3cla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cla ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CAT3_ECOLX CAT3_ECOLX]] This enzyme is an effector of chloramphenicol resistance in bacteria.	+	[https://www.uniprot.org/uniprot/CAT3_ECOLX CAT3_ECOLX] This enzyme is an effector of chloramphenicol resistance in bacteria.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cla ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cla ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	High level bacterial resistance to chloramphenicol is generally due to O-acetylation of the antibiotic in a reaction catalysed by chloramphenicol acetyltransferase (CAT, EC 2.3.1.28) in which acetyl-coenzyme A is the acyl donor. The crystal structure of the type III enzyme from Escherichia coli with chloramphenicol bound has been determined and refined at 1.75 A resolution, using a restrained parameter reciprocal space least squares procedure. The refined model, which includes chloramphenicol, 204 solvent molecules and two cobalt ions has a crystallographic R-factor of 18.3% for 27,300 reflections between 6 and 1.75 A resolution. The root-mean-square deviation in bond lengths from ideal values is 0.02 A. The cobalt ions play a crucial role in stabilizing the packing of the molecule in the crystal lattice. CAT is a trimer of identical subunits (monomer Mr 25,000) and the trimeric structure is stabilized by a number of hydrogen bonds, some of which result in the extension of a beta-sheet across the subunit interface. Chloramphenicol binds in a deep pocket located at the boundary between adjacent subunits of the trimer, such that the majority of residues forming the binding pocket belong to one subunit while the catalytically essential histidine belongs to the adjacent subunit. His195 is appropriately positioned to act as a general base catalyst in the reaction, and the required tautomeric stabilization is provided by an unusual interaction with a main-chain carbonyl oxygen.
-
-	Refined crystal structure of type III chloramphenicol acetyltransferase at 1.75 A resolution.,Leslie AG J Mol Biol. 1990 May 5;213(1):167-86. PMID:2187098<ref>PMID:2187098</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3cla" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Chloramphenicol acetyltransferase\|Chloramphenicol acetyltransferase]]		*[[Chloramphenicol acetyltransferase\|Chloramphenicol acetyltransferase]]
	*[[Chloramphenicol acetyltransferase 3D structures\|Chloramphenicol acetyltransferase 3D structures]]		*[[Chloramphenicol acetyltransferase 3D structures\|Chloramphenicol acetyltransferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Chloramphenicol O-acetyltransferase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Leslie~~, A G.W~~]]	+	[[Category: Leslie AGW]]

OCA at 10:27, 12 August 2020

2020-08-12T10:27:57Z

←Older revision		Revision as of 10:27, 12 August 2020
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	==REFINED CRYSTAL STRUCTURE OF TYPE III CHLORAMPHENICOL ACETYLTRANSFERASE AT 1.75 ANGSTROMS RESOLUTION==		==REFINED CRYSTAL STRUCTURE OF TYPE III CHLORAMPHENICOL ACETYLTRANSFERASE AT 1.75 ANGSTROMS RESOLUTION==
-	<StructureSection load='3cla' size='340' side='right' caption='[[3cla]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='3cla' size='340' side='right'caption='[[3cla]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cla]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CLA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cla]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3CLA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloramphenicol_O-acetyltransferase Chloramphenicol O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.28 2.3.1.28] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloramphenicol_O-acetyltransferase Chloramphenicol O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.28 2.3.1.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3cla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cla OCA], [http://pdbe.org/3cla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cla RCSB], [http://www.ebi.ac.uk/pdbsum/3cla PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cla ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3cla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cla OCA], [http://pdbe.org/3cla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cla RCSB], [http://www.ebi.ac.uk/pdbsum/3cla PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cla ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 32:		Line 32:
	==See Also==		==See Also==
	*[[Chloramphenicol acetyltransferase\|Chloramphenicol acetyltransferase]]		*[[Chloramphenicol acetyltransferase\|Chloramphenicol acetyltransferase]]
		+	*[[Chloramphenicol acetyltransferase 3D structures\|Chloramphenicol acetyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 39:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Chloramphenicol O-acetyltransferase]]		[[Category: Chloramphenicol O-acetyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Leslie, A G.W]]		[[Category: Leslie, A G.W]]

OCA at 13:46, 7 November 2018

2018-11-07T13:46:25Z

←Older revision		Revision as of 13:46, 7 November 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3cla_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3cla_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 19:01, 5 August 2016

2016-08-05T19:01:07Z

←Older revision		Revision as of 19:01, 5 August 2016
Line 1:		Line 1:
		+
	==REFINED CRYSTAL STRUCTURE OF TYPE III CHLORAMPHENICOL ACETYLTRANSFERASE AT 1.75 ANGSTROMS RESOLUTION==		==REFINED CRYSTAL STRUCTURE OF TYPE III CHLORAMPHENICOL ACETYLTRANSFERASE AT 1.75 ANGSTROMS RESOLUTION==
	<StructureSection load='3cla' size='340' side='right' caption='[[3cla]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3cla' size='340' side='right' caption='[[3cla]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cla]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cla]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloramphenicol_O-acetyltransferase Chloramphenicol O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.28 2.3.1.28] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloramphenicol_O-acetyltransferase Chloramphenicol O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.28 2.3.1.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cla OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cla RCSB], [http://www.ebi.ac.uk/pdbsum/3cla PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cla OCA], [http://pdbe.org/3cla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cla RCSB], [http://www.ebi.ac.uk/pdbsum/3cla PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cla ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~CAT3_ECOLI CAT3_ECOLI~~]] This enzyme is an effector of chloramphenicol resistance in bacteria.	+	[[http://www.uniprot.org/uniprot/CAT3_ECOLX CAT3_ECOLX]] This enzyme is an effector of chloramphenicol resistance in bacteria.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cla ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3cla" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Chloramphenicol O-acetyltransferase]]		[[Category: Chloramphenicol O-acetyltransferase]]
-	[[Category: Escherichia coli]]
	[[Category: Leslie, A G.W]]		[[Category: Leslie, A G.W]]

OCA at 21:09, 25 December 2014

2014-12-25T21:09:56Z

←Older revision		Revision as of 21:09, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3cla]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLA FirstGlance]. <br>		<table><tr><td colspan='2'>[[3cla]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloramphenicol_O-acetyltransferase Chloramphenicol O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.28 2.3.1.28] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloramphenicol_O-acetyltransferase Chloramphenicol O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.28 2.3.1.28] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cla OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cla RCSB], [http://www.ebi.ac.uk/pdbsum/3cla PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cla OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cla RCSB], [http://www.ebi.ac.uk/pdbsum/3cla PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAT3_ECOLI CAT3_ECOLI]] This enzyme is an effector of chloramphenicol resistance in bacteria.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Chloramphenicol O-acetyltransferase]]		[[Category: Chloramphenicol O-acetyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Leslie, A G.W.]]	+	[[Category: Leslie, A G.W]]

OCA at 06:56, 29 September 2014

2014-09-29T06:56:12Z

←Older revision		Revision as of 06:56, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:3cla.~~png\|left~~\|200px]]	+	==REFINED CRYSTAL STRUCTURE OF TYPE III CHLORAMPHENICOL ACETYLTRANSFERASE AT 1.75 ANGSTROMS RESOLUTION==
		+	<StructureSection load='3cla' size='340' side='right' caption='[[3cla]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3cla]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloramphenicol_O-acetyltransferase Chloramphenicol O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.28 2.3.1.28] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cla OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cla RCSB], [http://www.ebi.ac.uk/pdbsum/3cla PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3cla_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	High level bacterial resistance to chloramphenicol is generally due to O-acetylation of the antibiotic in a reaction catalysed by chloramphenicol acetyltransferase (CAT, EC 2.3.1.28) in which acetyl-coenzyme A is the acyl donor. The crystal structure of the type III enzyme from Escherichia coli with chloramphenicol bound has been determined and refined at 1.75 A resolution, using a restrained parameter reciprocal space least squares procedure. The refined model, which includes chloramphenicol, 204 solvent molecules and two cobalt ions has a crystallographic R-factor of 18.3% for 27,300 reflections between 6 and 1.75 A resolution. The root-mean-square deviation in bond lengths from ideal values is 0.02 A. The cobalt ions play a crucial role in stabilizing the packing of the molecule in the crystal lattice. CAT is a trimer of identical subunits (monomer Mr 25,000) and the trimeric structure is stabilized by a number of hydrogen bonds, some of which result in the extension of a beta-sheet across the subunit interface. Chloramphenicol binds in a deep pocket located at the boundary between adjacent subunits of the trimer, such that the majority of residues forming the binding pocket belong to one subunit while the catalytically essential histidine belongs to the adjacent subunit. His195 is appropriately positioned to act as a general base catalyst in the reaction, and the required tautomeric stabilization is provided by an unusual interaction with a main-chain carbonyl oxygen.

-	~~{{STRUCTURE_3cla\| PDB=3cla \| SCENE= }}~~	+	Refined crystal structure of type III chloramphenicol acetyltransferase at 1.75 A resolution.,Leslie AG J Mol Biol. 1990 May 5;213(1):167-86. PMID:2187098<ref>PMID:2187098</ref>

-	~~===REFINED CRYSTAL STRUCTURE OF TYPE III CHLORAMPHENICOL ACETYLTRANSFERASE AT 1.75 ANGSTROMS RESOLUTION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_2187098}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3cla]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA].~~	+

	==See Also==		==See Also==
	*[[Chloramphenicol acetyltransferase\|Chloramphenicol acetyltransferase]]		*[[Chloramphenicol acetyltransferase\|Chloramphenicol acetyltransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:002187098<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Chloramphenicol O-acetyltransferase]]		[[Category: Chloramphenicol O-acetyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Leslie, A G.W.]]		[[Category: Leslie, A G.W.]]

OCA at 21:41, 20 October 2012

2012-10-20T21:41:12Z

←Older revision		Revision as of 21:41, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3cla]] is a 1 chain structure ~~of [[Chloramphenicol acetyltransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA].	+	[[3cla]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:002187098~~</ref><ref group="xtra">PMID:011917145~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:002187098</ref><references group="xtra"/>
	[[Category: Chloramphenicol O-acetyltransferase]]		[[Category: Chloramphenicol O-acetyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Leslie, A G.W.]]		[[Category: Leslie, A G.W.]]

OCA: Protected "3cla" [edit=sysop:move=sysop]

2012-07-27T13:58:46Z

Protected "3cla" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:58, 27 July 2012

OCA at 13:58, 27 July 2012

2012-07-27T13:58:45Z

←Older revision		Revision as of 13:58, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3cla.png\|left\|200px]]		[[Image:3cla.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3cla", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3cla\| PDB=3cla \| SCENE= }}		{{STRUCTURE_3cla\| PDB=3cla \| SCENE= }}

	===REFINED CRYSTAL STRUCTURE OF TYPE III CHLORAMPHENICOL ACETYLTRANSFERASE AT 1.75 ANGSTROMS RESOLUTION===		===REFINED CRYSTAL STRUCTURE OF TYPE III CHLORAMPHENICOL ACETYLTRANSFERASE AT 1.75 ANGSTROMS RESOLUTION===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_2187098}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 2187098 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_2187098}}		{{ABSTRACT_PUBMED_2187098}}

	==About this Structure==		==About this Structure==
-	~~3CLA~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA].	+	[[3cla]] is a 1 chain structure of [[Chloramphenicol acetyltransferase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA].
		+
		+	==See Also==
		+	*[[Chloramphenicol acetyltransferase\|Chloramphenicol acetyltransferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~2187098~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:002187098</ref><ref group="xtra">PMID:011917145</ref><references group="xtra"/>
	[[Category: Chloramphenicol O-acetyltransferase]]		[[Category: Chloramphenicol O-acetyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Leslie, A G.W.]]		[[Category: Leslie, A G.W.]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:41:09 2009''

OCA at 05:41, 18 February 2009

2009-02-18T05:41:10Z

←Older revision		Revision as of 05:41, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3CLA is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA].	+	3CLA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLA OCA].

	==Reference==		==Reference==
-	~~Refined crystal structure of type III chloramphenicol acetyltransferase at 1.75 A resolution., Leslie AG, J Mol Biol. 1990 May 5;213(1):167-86.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/2187098 2187098]~~	+	<ref group="xtra">PMID:2187098</ref><references group="xtra"/>
	[[Category: Chloramphenicol O-acetyltransferase]]		[[Category: Chloramphenicol O-acetyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Leslie, A G.W.]]		[[Category: Leslie, A G.W.]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 14:~~07:~~19 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:41:09 2009''