3clc - Revision history

OCA at 09:36, 21 February 2024

2024-02-21T09:36:26Z

←Older revision		Revision as of 09:36, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3clc' size='340' side='right'caption='[[3clc]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3clc' size='340' side='right'caption='[[3clc]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3clc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_sp. Enterobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3clc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_sp._RFL1396 Enterobacter sp. RFL1396]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~esp1396IC ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42895 Enterobacter sp.])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clc OCA], [https://pdbe.org/3clc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3clc RCSB], [https://www.ebi.ac.uk/pdbsum/3clc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3clc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clc OCA], [https://pdbe.org/3clc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3clc RCSB], [https://www.ebi.ac.uk/pdbsum/3clc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3clc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8GGH0_9ENTR Q8GGH0_9ENTR]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3clc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3clc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Controller (C) proteins regulate the timing of the expression of restriction and modification (R-M) genes through a combination of positive and negative feedback circuits. A single dimer bound to the operator switches on transcription of the C-gene and the endonuclease gene; at higher concentrations, a second dimer bound adjacently switches off these genes. Here we report the first structure of a C protein-DNA operator complex, consisting of two C protein dimers bound to the native 35 bp operator sequence of the R-M system Esp1396I. The structure reveals a role for both direct and indirect DNA sequence recognition. The structure of the DNA in the complex is highly distorted, with severe compression of the minor groove resulting in a 50 degrees bend within each operator site, together with a large expansion of the major groove in the centre of the DNA sequence. Cooperative binding between dimers governs the concentration-dependent activation-repression switch and arises, in part, from the interaction of Glu25 and Arg35 side chains at the dimer-dimer interface. Competition between Arg35 and an equivalent residue of the sigma(70) subunit of RNA polymerase for the Glu25 site underpins the switch from activation to repression of the endonuclease gene.
-
-	Structural analysis of the genetic switch that regulates the expression of restriction-modification genes.,McGeehan JE, Streeter SD, Thresh SJ, Ball N, Ravelli RB, Kneale GG Nucleic Acids Res. 2008 Aug;36(14):4778-87. Epub 2008 Jul 21. PMID:18644840<ref>PMID:18644840</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3clc" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterobacter sp]]	+	[[Category: Enterobacter sp. RFL1396]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ball, N]]	+	[[Category: Ball N]]
-	[[Category: Kneale~~, G G~~]]	+	[[Category: Kneale GG]]
-	[[Category: McGeehan~~, J E~~]]	+	[[Category: McGeehan JE]]
-	[[Category: Ravelli~~, R B~~]]	+	[[Category: Ravelli RB]]
-	[[Category: Streeter~~, S D~~]]	+	[[Category: Streeter SD]]
-	[[Category: Thresh~~, S J]]~~	+	[[Category: Thresh SJ]]
-	~~[[Category: Dna-bending]]~~	+
-	~~[[Category: Helix-turn-helix]]~~	+
-	~~[[Category: Protein-dna complex]]~~	+
-	~~[[Category: Transcription regulator-dna complex]]~~	+
-	~~[[Category: Transcriptional regulator~~]]	+

OCA at 08:00, 27 January 2022

2022-01-27T08:00:14Z

←Older revision		Revision as of 08:00, 27 January 2022
Line 1:		Line 1:

	==Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator==		==Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator==
-	<StructureSection load='3clc' size='340' side='right' caption='[[3clc]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='3clc' size='340' side='right'caption='[[3clc]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3clc]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Enterobacter_sp. Enterobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CLC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3clc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_sp. Enterobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">esp1396IC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42895 Enterobacter sp.])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">esp1396IC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42895 Enterobacter sp.])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clc OCA], [~~http~~://pdbe.org/3clc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3clc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3clc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3clc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clc OCA], [https://pdbe.org/3clc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3clc RCSB], [https://www.ebi.ac.uk/pdbsum/3clc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3clc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</StructureSection>		</StructureSection>
	[[Category: Enterobacter sp]]		[[Category: Enterobacter sp]]
		+	[[Category: Large Structures]]
	[[Category: Ball, N]]		[[Category: Ball, N]]
	[[Category: Kneale, G G]]		[[Category: Kneale, G G]]

OCA at 13:17, 7 November 2018

2018-11-07T13:17:43Z

←Older revision		Revision as of 13:17, 7 November 2018
Line 1:		Line 1:
		+
	==Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator==		==Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator==
	<StructureSection load='3clc' size='340' side='right' caption='[[3clc]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3clc' size='340' side='right' caption='[[3clc]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">esp1396IC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42895 Enterobacter sp.])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">esp1396IC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42895 Enterobacter sp.])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clc OCA], [http://pdbe.org/3clc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3clc RCSB], [http://www.ebi.ac.uk/pdbsum/3clc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clc OCA], [http://pdbe.org/3clc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3clc RCSB], [http://www.ebi.ac.uk/pdbsum/3clc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3clc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3clc_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3clc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 14:28, 9 February 2016

2016-02-09T14:28:14Z

←Older revision		Revision as of 14:28, 9 February 2016
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">esp1396IC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42895 Enterobacter sp.])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">esp1396IC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42895 Enterobacter sp.])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clc RCSB], [http://www.ebi.ac.uk/pdbsum/3clc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clc OCA], [http://pdbe.org/3clc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3clc RCSB], [http://www.ebi.ac.uk/pdbsum/3clc PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3clc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3clc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:56, 20 January 2015

2015-01-20T08:56:53Z

←Older revision		Revision as of 08:56, 20 January 2015
Line 29:		Line 29:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterobacter sp.]]	+	[[Category: Enterobacter sp]]
-	[[Category: Ball, N.]]	+	[[Category: Ball, N]]
-	[[Category: Kneale, G G.]]	+	[[Category: Kneale, G G]]
-	[[Category: McGeehan, J E.]]	+	[[Category: McGeehan, J E]]
-	[[Category: Ravelli, R B.]]	+	[[Category: Ravelli, R B]]
-	[[Category: Streeter, S D.]]	+	[[Category: Streeter, S D]]
-	[[Category: Thresh, S J.]]	+	[[Category: Thresh, S J]]
	[[Category: Dna-bending]]		[[Category: Dna-bending]]
	[[Category: Helix-turn-helix]]		[[Category: Helix-turn-helix]]

OCA at 06:24, 10 October 2014

2014-10-10T06:24:51Z

←Older revision		Revision as of 06:24, 10 October 2014
Line 1:		Line 1:
-	[[~~Image:~~3clc.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator==
		+	<StructureSection load='3clc' size='340' side='right' caption='[[3clc]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3clc]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_sp. Enterobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">esp1396IC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42895 Enterobacter sp.])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clc RCSB], [http://www.ebi.ac.uk/pdbsum/3clc PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3clc_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Controller (C) proteins regulate the timing of the expression of restriction and modification (R-M) genes through a combination of positive and negative feedback circuits. A single dimer bound to the operator switches on transcription of the C-gene and the endonuclease gene; at higher concentrations, a second dimer bound adjacently switches off these genes. Here we report the first structure of a C protein-DNA operator complex, consisting of two C protein dimers bound to the native 35 bp operator sequence of the R-M system Esp1396I. The structure reveals a role for both direct and indirect DNA sequence recognition. The structure of the DNA in the complex is highly distorted, with severe compression of the minor groove resulting in a 50 degrees bend within each operator site, together with a large expansion of the major groove in the centre of the DNA sequence. Cooperative binding between dimers governs the concentration-dependent activation-repression switch and arises, in part, from the interaction of Glu25 and Arg35 side chains at the dimer-dimer interface. Competition between Arg35 and an equivalent residue of the sigma(70) subunit of RNA polymerase for the Glu25 site underpins the switch from activation to repression of the endonuclease gene.

-	~~{{STRUCTURE_3clc\| PDB=3clc \| SCENE= }}~~	+	Structural analysis of the genetic switch that regulates the expression of restriction-modification genes.,McGeehan JE, Streeter SD, Thresh SJ, Ball N, Ravelli RB, Kneale GG Nucleic Acids Res. 2008 Aug;36(14):4778-87. Epub 2008 Jul 21. PMID:18644840<ref>PMID:18644840</ref>

-	~~===Crystal Structure~~ of the ~~Restriction-Modification Controller Protein C~~.~~Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18644840}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3clc]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Enterobacter_sp. Enterobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018644840<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterobacter sp.]]		[[Category: Enterobacter sp.]]
	[[Category: Ball, N.]]		[[Category: Ball, N.]]

OCA: Protected "3clc" [edit=sysop:move=sysop]

2013-01-07T18:30:56Z

Protected "3clc" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:30, 7 January 2013

OCA at 18:30, 7 January 2013

2013-01-07T18:30:54Z

←Older revision		Revision as of 18:30, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3clc.png\|left\|200px]]		[[Image:3clc.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3clc", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3clc\| PDB=3clc \| SCENE= }}		{{STRUCTURE_3clc\| PDB=3clc \| SCENE= }}

	===Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator===		===Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18644840}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18644840 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18644840}}		{{ABSTRACT_PUBMED_18644840}}

	==About this Structure==		==About this Structure==
-	~~3CLC~~ is a 6 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Enterobacter_sp.~~_rfl1396~~ Enterobacter sp. ~~rfl1396~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA].	+	[[3clc]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_sp. Enterobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18644840~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018644840</ref><references group="xtra"/>
-	[[Category: Enterobacter sp. ~~rfl1396~~]]	+	[[Category: Enterobacter sp.]]
	[[Category: Ball, N.]]		[[Category: Ball, N.]]
	[[Category: Kneale, G G.]]		[[Category: Kneale, G G.]]
Line 33:		Line 21:
	[[Category: Dna-bending]]		[[Category: Dna-bending]]
	[[Category: Helix-turn-helix]]		[[Category: Helix-turn-helix]]
-	[[Category: Plasmid]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
-	[[Category: Transcription regulator/dna complex]]	+	[[Category: Transcription regulator-dna complex]]
	[[Category: Transcriptional regulator]]		[[Category: Transcriptional regulator]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:29:05 2009''

OCA at 08:29, 17 February 2009

2009-02-17T08:29:06Z

←Older revision		Revision as of 08:29, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3CLC is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Enterobacter_sp._rfl1396 Enterobacter sp. rfl1396]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA].	+	3CLC is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterobacter_sp._rfl1396 Enterobacter sp. rfl1396]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA].

	==Reference==		==Reference==
-	Structural analysis of the genetic switch that regulates the expression of restriction-modification genes., McGeehan JE, Streeter SD, Thresh SJ, Ball N, Ravelli RB, Kneale GG, Nucleic Acids Res. 2008 Aug;36(14):4778-87. Epub 2008 Jul 21. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18644840 18644840]~~	+	<ref group="xtra">PMID:18644840</ref><references group="xtra"/>
	[[Category: Enterobacter sp. rfl1396]]		[[Category: Enterobacter sp. rfl1396]]
-	[[Category: Protein complex]]
	[[Category: Ball, N.]]		[[Category: Ball, N.]]
	[[Category: Kneale, G G.]]		[[Category: Kneale, G G.]]
Line 39:		Line 38:
	[[Category: Transcriptional regulator]]		[[Category: Transcriptional regulator]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Aug 20 11~~:22:~~34 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:29:05 2009''

OCA at 08:22, 20 August 2008

2008-08-20T08:22:35Z

←Older revision		Revision as of 08:22, 20 August 2008
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3clc.png\|left\|200px]]

-	The ~~entry 3clc~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3clc", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3clc\| PDB=3clc \| SCENE= }}

-	~~Authors: McGeehan, J.E., Streeter, S.D., Thresh, S.J., Ball, N.J., Ravelli, R.B.G., Kneale, G.G~~.	+	===Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator===

-	Description: Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator

		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18644840}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18644840 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18644840}}

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jun~~ 11 09:32:33 2008''	+	==About this Structure==
		+	3CLC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacter_sp._rfl1396 Enterobacter sp. rfl1396]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLC OCA].
		+
		+	==Reference==
		+	Structural analysis of the genetic switch that regulates the expression of restriction-modification genes., McGeehan JE, Streeter SD, Thresh SJ, Ball N, Ravelli RB, Kneale GG, Nucleic Acids Res. 2008 Aug;36(14):4778-87. Epub 2008 Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18644840 18644840]
		+	[[Category: Enterobacter sp. rfl1396]]
		+	[[Category: Protein complex]]
		+	[[Category: Ball, N.]]
		+	[[Category: Kneale, G G.]]
		+	[[Category: McGeehan, J E.]]
		+	[[Category: Ravelli, R B.]]
		+	[[Category: Streeter, S D.]]
		+	[[Category: Thresh, S J.]]
		+	[[Category: Dna-bending]]
		+	[[Category: Helix-turn-helix]]
		+	[[Category: Plasmid]]
		+	[[Category: Protein-dna complex]]
		+	[[Category: Transcription regulator/dna complex]]
		+	[[Category: Transcriptional regulator]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 20 11:22:34 2008''