3cux - Revision history

OCA at 09:38, 21 February 2024

2024-02-21T09:38:35Z

←Older revision		Revision as of 09:38, 21 February 2024
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	<StructureSection load='3cux' size='340' side='right'caption='[[3cux]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3cux' size='340' side='right'caption='[[3cux]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cux]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var.~~ anthracis~~" (cohn 1872) smith et al. 1946~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cux]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CUX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1d8c\|1d8c]]~~, [[~~3cuz~~\|~~3cuz]], [[3cv1\|3cv1]], [[3cv2\|3cv2~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>aceB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1392~~ "~~Bacillus cereus var. anthracis~~" ~~(Cohn 1872) Smith et al. 1946])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cux OCA], [https://pdbe.org/3cux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cux RCSB], [https://www.ebi.ac.uk/pdbsum/3cux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cux ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cux OCA], [https://pdbe.org/3cux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cux RCSB], [https://www.ebi.ac.uk/pdbsum/3cux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cux ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6L8PPD0_BACAN A0A6L8PPD0_BACAN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cux ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cux ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Enzymes of the glyoxylate shunt are important for the virulence of pathogenic organisms such as Mycobacterium tuberculosis and Candida albicans. Two isoforms have been identified for malate synthase, the second enzyme in the pathway. Isoform A, found in fungi and plants, comprises approximately 530 residues, whereas isoform G, found only in bacteria, is larger by approximately 200 residues. Crystal structures of malate synthase isoform G from Escherichia coli and Mycobacterium tuberculosis were previously determined at moderate resolution. Here we describe crystal structures of E. coli malate synthase A (MSA) in the apo form (1.04 A resolution) and in complex with acetyl-coenzyme A and a competitive inhibitor, possibly pyruvate or oxalate (1.40 A resolution). In addition, a crystal structure for Bacillus anthracis MSA at 1.70 A resolution is reported. The increase in size between isoforms A and G can be attributed primarily to an inserted alpha/beta domain that may have regulatory function. Upon binding of inhibitor or substrate, several active site loops in MSA undergo large conformational changes. However, in the substrate bound form, the active sites of isoforms A and G from E. coli are nearly identical. Considering that inhibitors bind with very similar affinities to both isoforms, MSA is as an excellent platform for high-resolution structural studies and drug discovery efforts.
-
-	Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery.,Lohman JR, Olson AC, Remington SJ Protein Sci. 2008 Nov;17(11):1935-45. Epub 2008 Aug 19. PMID:18714089<ref>PMID:18714089</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3cux" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Malate synthase 3D structures\|Malate synthase 3D structures]]		*[[Malate synthase 3D structures\|Malate synthase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus anthracis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Malate synthase]]~~	+	[[Category: Lohman JR]]
-	[[Category: Lohman~~, J R~~]]	+	[[Category: Remington SJ]]
-	[[Category: Remington~~, S J]]~~	+
-	~~[[Category: Glyoxylate bypass]]~~	+
-	~~[[Category: Tim barrel]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Tricarboxylic acid cycle~~]]	+

OCA at 07:46, 2 February 2022

2022-02-02T07:46:02Z

←Older revision		Revision as of 07:46, 2 February 2022
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	==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==		==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==
-	<StructureSection load='3cux' size='340' side='right' caption='[[3cux]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3cux' size='340' side='right'caption='[[3cux]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cux]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cux]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CUX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8c\|1d8c]], [[3cuz\|3cuz]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d8c\|1d8c]], [[3cuz\|3cuz]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3cux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cux OCA], [~~http~~://pdbe.org/3cux PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3cux RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3cux PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3cux ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cux OCA], [https://pdbe.org/3cux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cux RCSB], [https://www.ebi.ac.uk/pdbsum/3cux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cux ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
-	*[[Malate synthase\|Malate synthase]]	+	*[[Malate synthase 3D structures\|Malate synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
	[[Category: Lohman, J R]]		[[Category: Lohman, J R]]

OCA at 14:00, 7 November 2018

2018-11-07T14:00:38Z

←Older revision		Revision as of 14:00, 7 November 2018
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		+
	==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==		==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==
	<StructureSection load='3cux' size='340' side='right' caption='[[3cux]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3cux' size='340' side='right' caption='[[3cux]], [[Resolution\|resolution]] 1.70Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cux OCA], [http://pdbe.org/3cux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cux RCSB], [http://www.ebi.ac.uk/pdbsum/3cux PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cux OCA], [http://pdbe.org/3cux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cux RCSB], [http://www.ebi.ac.uk/pdbsum/3cux PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cux ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/3cux_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/3cux_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:55, 8 February 2016

2016-02-08T13:55:02Z

←Older revision		Revision as of 13:55, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3cux' size='340' side='right' caption='[[3cux]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3cux' size='340' side='right' caption='[[3cux]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cux]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_anthracis Bacillus~~ anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cux]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CUX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8c\|1d8c]], [[3cuz\|3cuz]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8c\|1d8c]], [[3cuz\|3cuz]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cux OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cux RCSB], [http://www.ebi.ac.uk/pdbsum/3cux PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cux OCA], [http://pdbe.org/3cux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cux RCSB], [http://www.ebi.ac.uk/pdbsum/3cux PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cux ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3cux" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus anthracis]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
	[[Category: Lohman, J R]]		[[Category: Lohman, J R]]

OCA at 10:52, 20 January 2015

2015-01-20T10:52:13Z

←Older revision		Revision as of 10:52, 20 January 2015
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	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
-	[[Category: Lohman, J R.]]	+	[[Category: Lohman, J R]]
-	[[Category: Remington, S J.]]	+	[[Category: Remington, S J]]
	[[Category: Glyoxylate bypass]]		[[Category: Glyoxylate bypass]]
-	[[Category: Malate synthase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]

OCA at 06:15, 10 October 2014

2014-10-10T06:15:54Z

←Older revision		Revision as of 06:15, 10 October 2014
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-	[[~~Image:~~3cux.~~png~~\|~~left~~\|200px]]	+	==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==
		+	<StructureSection load='3cux' size='340' side='right' caption='[[3cux]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3cux]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CUX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8c\|1d8c]], [[3cuz\|3cuz]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cux OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cux RCSB], [http://www.ebi.ac.uk/pdbsum/3cux PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/3cux_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymes of the glyoxylate shunt are important for the virulence of pathogenic organisms such as Mycobacterium tuberculosis and Candida albicans. Two isoforms have been identified for malate synthase, the second enzyme in the pathway. Isoform A, found in fungi and plants, comprises approximately 530 residues, whereas isoform G, found only in bacteria, is larger by approximately 200 residues. Crystal structures of malate synthase isoform G from Escherichia coli and Mycobacterium tuberculosis were previously determined at moderate resolution. Here we describe crystal structures of E. coli malate synthase A (MSA) in the apo form (1.04 A resolution) and in complex with acetyl-coenzyme A and a competitive inhibitor, possibly pyruvate or oxalate (1.40 A resolution). In addition, a crystal structure for Bacillus anthracis MSA at 1.70 A resolution is reported. The increase in size between isoforms A and G can be attributed primarily to an inserted alpha/beta domain that may have regulatory function. Upon binding of inhibitor or substrate, several active site loops in MSA undergo large conformational changes. However, in the substrate bound form, the active sites of isoforms A and G from E. coli are nearly identical. Considering that inhibitors bind with very similar affinities to both isoforms, MSA is as an excellent platform for high-resolution structural studies and drug discovery efforts.

-	~~{{STRUCTURE_3cux\| PDB=3cux \| SCENE= }}~~	+	Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery.,Lohman JR, Olson AC, Remington SJ Protein Sci. 2008 Nov;17(11):1935-45. Epub 2008 Aug 19. PMID:18714089<ref>PMID:18714089</ref>

-	~~===Atomic Resolution Structures~~ of ~~Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_18714089}}~~	+	==See Also==
-		+	*[[Malate synthase\|Malate synthase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3cux~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:018714089<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]

OCA: Protected "3cux" [edit=sysop:move=sysop]

2013-01-07T19:45:59Z

Protected "3cux" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:45, 7 January 2013

OCA at 19:45, 7 January 2013

2013-01-07T19:45:57Z

←Older revision		Revision as of 19:45, 7 January 2013
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-	{{Seed}}
	[[Image:3cux.png\|left\|200px]]		[[Image:3cux.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3cux", creates the "Structure Box" on the page.
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-	-->
	{{STRUCTURE_3cux\| PDB=3cux \| SCENE= }}		{{STRUCTURE_3cux\| PDB=3cux \| SCENE= }}

	===Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design===		===Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18714089}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18714089 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18714089}}		{{ABSTRACT_PUBMED_18714089}}

	==About this Structure==		==About this Structure==
-	~~3CUX~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA].	+	[[3cux]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18714089~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018714089</ref><references group="xtra"/>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
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	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:11:12 2009''

OCA at 21:11, 16 February 2009

2009-02-16T21:11:16Z

←Older revision		Revision as of 21:11, 16 February 2009
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	{{Seed}}		{{Seed}}
-	[[Image:3cux.~~jpg~~\|left\|200px]]	+	[[Image:3cux.png\|left\|200px]]

	<!--		<!--
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	==Reference==		==Reference==
-	Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery., Lohman JR, Olson AC, Remington SJ, Protein Sci. 2008 Nov;17(11):1935-45. Epub 2008 Aug 19. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18714089 18714089]~~	+	<ref group="xtra">PMID:18714089</ref><references group="xtra"/>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
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	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Nov 12 10~~:46:~~21 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:11:12 2009''

OCA at 08:46, 12 November 2008

2008-11-12T08:46:22Z

←Older revision		Revision as of 08:46, 12 November 2008
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3cux.jpg\|left\|200px]]

-	The ~~entry 3cux~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3cux", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3cux\| PDB=3cux \| SCENE= }}

-	~~Authors~~: ~~Lohman, J.R., Remington, S.J.~~	+	===Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design===

-	Description: Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri Jul 11 13~~:04:15 2008''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18714089}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18714089 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18714089}}
		+
		+	==About this Structure==
		+	3CUX is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUX OCA].
		+
		+	==Reference==
		+	Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery., Lohman JR, Olson AC, Remington SJ, Protein Sci. 2008 Nov;17(11):1935-45. Epub 2008 Aug 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18714089 18714089]
		+	[[Category: Bacillus anthracis]]
		+	[[Category: Malate synthase]]
		+	[[Category: Lohman, J R.]]
		+	[[Category: Remington, S J.]]
		+	[[Category: Glyoxylate bypass]]
		+	[[Category: Malate synthase]]
		+	[[Category: Tim barrel]]
		+	[[Category: Transferase]]
		+	[[Category: Tricarboxylic acid cycle]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 12 10:46:21 2008''