3eao - Revision history

OCA at 13:00, 30 August 2023

2023-08-30T13:00:15Z

←Older revision		Revision as of 13:00, 30 August 2023
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	<StructureSection load='3eao' size='340' side='right'caption='[[3eao]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='3eao' size='340' side='right'caption='[[3eao]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3eao]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EAO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3eao]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EAO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~, ~~<scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1h6v\|1h6v]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Txnrd1, Trxr1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10116 Buffalo rat])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Thioredoxin~~-~~disulfide_reductase Thioredoxin~~-~~disulfide reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [https://pdbe.org/3eao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [https://www.ebi.ac.uk/pdbsum/3eao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eao ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [https://pdbe.org/3eao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [https://www.ebi.ac.uk/pdbsum/3eao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eao ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRXR1_RAT TRXR1_RAT]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buffalo rat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thioredoxin-disulfide reductase~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Arner, E]]	+	[[Category: Arner E]]
-	[[Category: Cheng, Q]]	+	[[Category: Cheng Q]]
-	[[Category: Lindqvist, Y]]	+	[[Category: Lindqvist Y]]
-	[[Category: Sandalova, T]]	+	[[Category: Sandalova T]]
-	~~[[Category: Fad]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Mammalian thioredoxin reductase 1]]~~	+
-	~~[[Category: Nadp]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Redox-active center]]~~	+
-	~~[[Category: Selenenylsulfide]]~~	+
-	~~[[Category: Selenium]]~~	+
-	~~[[Category: Selenocysteine]]~~	+
-	~~[[Category: Selenoprotein~~]]	+

OCA at 11:45, 16 February 2022

2022-02-16T11:45:04Z

←Older revision		Revision as of 11:45, 16 February 2022
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	==Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail==		==Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail==
-	<StructureSection load='3eao' size='340' side='right' caption='[[3eao]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='3eao' size='340' side='right'caption='[[3eao]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3eao]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3EAO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3eao]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EAO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1h6v\|1h6v]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1h6v\|1h6v]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Txnrd1, Trxr1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Txnrd1, Trxr1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [~~http~~://pdbe.org/3eao PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3eao PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3eao ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [https://pdbe.org/3eao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [https://www.ebi.ac.uk/pdbsum/3eao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eao ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Thioredoxin ~~Reductase~~\|Thioredoxin ~~Reductase~~]]	+	*[[Thioredoxin reductase 3D structures\|Thioredoxin reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
		+	[[Category: Large Structures]]
	[[Category: Thioredoxin-disulfide reductase]]		[[Category: Thioredoxin-disulfide reductase]]
	[[Category: Arner, E]]		[[Category: Arner, E]]

OCA at 05:40, 8 March 2018

2018-03-08T05:40:27Z

←Older revision		Revision as of 05:40, 8 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail==		==Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail==
	<StructureSection load='3eao' size='340' side='right' caption='[[3eao]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='3eao' size='340' side='right' caption='[[3eao]], [[Resolution\|resolution]] 3.10Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Txnrd1, Trxr1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Txnrd1, Trxr1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [http://pdbe.org/3eao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [http://www.ebi.ac.uk/pdbsum/3eao PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [http://pdbe.org/3eao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [http://www.ebi.ac.uk/pdbsum/3eao PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eao ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/3eao_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/3eao_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 01:11, 10 February 2016

2016-02-10T01:11:30Z

←Older revision		Revision as of 01:11, 10 February 2016
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Txnrd1, Trxr1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Txnrd1, Trxr1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [http://www.ebi.ac.uk/pdbsum/3eao PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [http://pdbe.org/3eao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [http://www.ebi.ac.uk/pdbsum/3eao PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eao ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3eao" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 14:22, 5 January 2015

2015-01-05T14:22:33Z

←Older revision		Revision as of 14:22, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eao]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAO FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eao]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAO FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1h6v\|1h6v]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1h6v\|1h6v]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Txnrd1, Trxr1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Txnrd1, Trxr1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [http://www.ebi.ac.uk/pdbsum/3eao PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [http://www.ebi.ac.uk/pdbsum/3eao PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
	[[Category: Thioredoxin-disulfide reductase]]		[[Category: Thioredoxin-disulfide reductase]]
-	[[Category: Arner, E.]]	+	[[Category: Arner, E]]
-	[[Category: Cheng, Q.]]	+	[[Category: Cheng, Q]]
-	[[Category: Lindqvist, Y.]]	+	[[Category: Lindqvist, Y]]
-	[[Category: Sandalova, T.]]	+	[[Category: Sandalova, T]]
	[[Category: Fad]]		[[Category: Fad]]
	[[Category: Flavoprotein]]		[[Category: Flavoprotein]]

OCA at 02:32, 7 August 2014

2014-08-07T02:32:56Z

←Older revision		Revision as of 02:32, 7 August 2014
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-	~~{{STRUCTURE_3eao\| PDB=3eao \| SCENE= }}~~	+	==Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail==
-	===Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail===	+	<StructureSection load='3eao' size='340' side='right' caption='[[3eao]], [[Resolution\|resolution]] 3.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19054767}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3eao]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAO FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1h6v\|1h6v]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Txnrd1, Trxr1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eao OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eao RCSB], [http://www.ebi.ac.uk/pdbsum/3eao PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/3eao_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Selenoproteins contain a highly reactive 21st amino acid selenocysteine (Sec) encoded by recoding of a predefined UGA codon. Because of a lack of selenoprotein supply, high chemical reactivity of Sec, and intricate translation machineries, selenoprotein crystal structures are difficult to obtain. Structural prerequisites for Sec involvement in enzyme catalysis are therefore sparsely known. Here we present the crystal structure of catalytically active rat thioredoxin reductase 1 (TrxR1), revealing surprises at the C-terminal Sec-containing active site in view of previous literature. The oxidized enzyme presents a selenenylsulfide motif in trans-configuration, with the selenium atom of Sec-498 positioned beneath the side chain of Tyr-116, thereby located far from the redox active moieties proposed to be involved in electron transport to the Sec-containing active site. Upon reduction to a selenolthiol motif, the Sec residue moved toward solvent exposure, consistent with its presumed role in reduction of TrxR1 substrates or as target of electrophilic agents inhibiting the enzyme. A Y116I mutation lowered catalytic efficiency in reduction of thioredoxin, but surprisingly increased turnover using 5-hydroxy-1,4-naphthoquinone (juglone) as substrate. The same mutation also decreased sensitivity to inhibition by cisplatin. The results suggest that Tyr-116 plays an important role for catalysis of TrxR1 by interacting with the selenenylsulfide of oxidized TrxR1, thereby facilitating its reduction in the reductive half-reaction of the enzyme. The interaction of a selenenylsulfide with the phenyl ring of a tyrosine, affecting turnover, switch of substrate specificity, and modulation of sensitivity to electrophilic agents, gives important clues into the mechanism of TrxR1, which is a selenoprotein that plays a major role for mammalian cell fate and function. The results also demonstrate that a recombinant selenoprotein TrxR can be produced in high amount and sufficient purity to enable crystal structure determination, which suggests that additional structural studies of these types of proteins are feasible.

-	~~==About this Structure==~~	+	Crystal structure and catalysis of the selenoprotein thioredoxin reductase 1.,Cheng Q, Sandalova T, Lindqvist Y, Arner ES J Biol Chem. 2009 Feb 6;284(6):3998-4008. Epub 2008 Dec 3. PMID:19054767<ref>PMID:19054767</ref>
-	~~[[3eao]] is a~~ 6 ~~chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3EAO OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Thioredoxin Reductase\|Thioredoxin Reductase]]		*[[Thioredoxin Reductase\|Thioredoxin Reductase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:019054767</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
	[[Category: Thioredoxin-disulfide reductase]]		[[Category: Thioredoxin-disulfide reductase]]

OCA at 14:43, 19 February 2014

2014-02-19T14:43:58Z

←Older revision		Revision as of 14:43, 19 February 2014
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-	[[Image:3eao.png\|left\|200px]]
-
	{{STRUCTURE_3eao\| PDB=3eao \| SCENE= }}		{{STRUCTURE_3eao\| PDB=3eao \| SCENE= }}
-
	===Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail===		===Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail===
-
	{{ABSTRACT_PUBMED_19054767}}		{{ABSTRACT_PUBMED_19054767}}

	==About this Structure==		==About this Structure==
-	[[3eao]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA].	+	[[3eao]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA].

	==See Also==		==See Also==
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:019054767</ref><~~ref~~ group="xtra"~~>PMID:010512699<~~/~~ref~~><references ~~group="xtra"~~/>	+	<ref group="xtra">PMID:019054767</ref><references group="xtra"/><references/>
-	[[Category: ~~Rattus norvegicus~~]]	+	[[Category: Buffalo rat]]
	[[Category: Thioredoxin-disulfide reductase]]		[[Category: Thioredoxin-disulfide reductase]]
	[[Category: Arner, E.]]		[[Category: Arner, E.]]

OCA at 15:47, 20 October 2012

2012-10-20T15:47:35Z

←Older revision		Revision as of 15:47, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3eao]] is a 6 chain structure ~~of [[Thioredoxin Reductase]]~~ with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA].	+	[[3eao]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAO OCA].

	==See Also==		==See Also==

OCA at 19:31, 26 July 2012

2012-07-26T19:31:06Z

←Older revision		Revision as of 19:31, 26 July 2012
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	[[Image:3eao.png\|left\|200px]]		[[Image:3eao.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3eao", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3eao\| PDB=3eao \| SCENE= }}		{{STRUCTURE_3eao\| PDB=3eao \| SCENE= }}

	===Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail===		===Crystal structure of recombinant rat selenoprotein thioredoxin reductase 1 with oxidized C-terminal tail===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19054767}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19054767 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_19054767}}		{{ABSTRACT_PUBMED_19054767}}

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	==See Also==		==See Also==
-	*[[Thioredoxin Reductase]]	+	*[[Thioredoxin Reductase\|Thioredoxin Reductase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19054767~~</ref><ref group="xtra">PMID:~~10512699~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019054767</ref><ref group="xtra">PMID:010512699</ref><references group="xtra"/>
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
	[[Category: Thioredoxin-disulfide reductase]]		[[Category: Thioredoxin-disulfide reductase]]
Line 32:		Line 21:
	[[Category: Lindqvist, Y.]]		[[Category: Lindqvist, Y.]]
	[[Category: Sandalova, T.]]		[[Category: Sandalova, T.]]
-	[[Category: Cytoplasm]]
	[[Category: Fad]]		[[Category: Fad]]
	[[Category: Flavoprotein]]		[[Category: Flavoprotein]]

OCA: Protected "3eao" [edit=sysop:move=sysop]

2011-03-14T23:25:04Z

Protected "3eao" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:25, 14 March 2011