3esk - Revision history

OCA at 06:32, 6 September 2023

2023-09-06T06:32:27Z

←Older revision		Revision as of 06:32, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3esk' size='340' side='right'caption='[[3esk]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='3esk' size='340' side='right'caption='[[3esk]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3esk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ESK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3esk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ESK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.05Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[1elr\|1elr]], [[1elw\|1elw]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">STIP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3esk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esk OCA], [https://pdbe.org/3esk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3esk RCSB], [https://www.ebi.ac.uk/pdbsum/3esk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3esk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3esk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esk OCA], [https://pdbe.org/3esk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3esk RCSB], [https://www.ebi.ac.uk/pdbsum/3esk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3esk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/STIP1_HUMAN STIP1_HUMAN]] Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). [[https://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN]] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>	+	[https://www.uniprot.org/uniprot/STIP1_HUMAN STIP1_HUMAN] Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kajander, T]]	+	[[Category: Kajander T]]
-	[[Category: Regan, L]]	+	[[Category: Regan L]]
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Hsc70]]~~	+
-	~~[[Category: Hsp90]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Stress response]]~~	+
-	~~[[Category: Tetratricopeptide repeat]]~~	+
-	~~[[Category: Tpr repeat]]~~	+
-	~~[[Category: Tpr2a~~]]	+

OCA at 09:15, 23 February 2022

2022-02-23T09:15:05Z

←Older revision		Revision as of 09:15, 23 February 2022
Line 1:		Line 1:

	==Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand==		==Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand==
-	<StructureSection load='3esk' size='340' side='right' caption='[[3esk]], [[Resolution\|resolution]] 2.05Å' scene=''>	+	<StructureSection load='3esk' size='340' side='right'caption='[[3esk]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3esk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ESK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3esk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ESK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1elr\|1elr]], [[1elw\|1elw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1elr\|1elr]], [[1elw\|1elw]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STIP1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STIP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3esk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esk OCA], [~~http~~://pdbe.org/3esk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3esk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3esk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3esk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3esk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esk OCA], [https://pdbe.org/3esk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3esk RCSB], [https://www.ebi.ac.uk/pdbsum/3esk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3esk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/STIP1_HUMAN STIP1_HUMAN]] Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). [[~~http~~://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN]] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>	+	[[https://www.uniprot.org/uniprot/STIP1_HUMAN STIP1_HUMAN]] Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). [[https://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN]] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Kajander, T]]		[[Category: Kajander, T]]
	[[Category: Regan, L]]		[[Category: Regan, L]]

OCA at 21:36, 2 December 2018

2018-12-02T21:36:01Z

←Older revision		Revision as of 21:36, 2 December 2018
Line 1:		Line 1:
		+
	==Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand==		==Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand==
	<StructureSection load='3esk' size='340' side='right' caption='[[3esk]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='3esk' size='340' side='right' caption='[[3esk]], [[Resolution\|resolution]] 2.05Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1elr\|1elr]], [[1elw\|1elw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1elr\|1elr]], [[1elw\|1elw]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3esk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esk OCA], [http://pdbe.org/3esk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3esk RCSB], [http://www.ebi.ac.uk/pdbsum/3esk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3esk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esk OCA], [http://pdbe.org/3esk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3esk RCSB], [http://www.ebi.ac.uk/pdbsum/3esk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3esk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/es/3esk_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/es/3esk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 3esk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3esk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[HOP protein\|HOP protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:40, 7 February 2016

2016-02-07T12:40:08Z

←Older revision		Revision as of 12:40, 7 February 2016
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1elr\|1elr]], [[1elw\|1elw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1elr\|1elr]], [[1elw\|1elw]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3esk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3esk RCSB], [http://www.ebi.ac.uk/pdbsum/3esk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3esk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esk OCA], [http://pdbe.org/3esk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3esk RCSB], [http://www.ebi.ac.uk/pdbsum/3esk PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3esk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3esk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:10, 24 December 2014

2014-12-24T15:10:38Z

←Older revision		Revision as of 15:10, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3esk\| PDB=3esk \| SCENE= }}~~	+	==Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand==
-	===Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand===	+	<StructureSection load='3esk' size='340' side='right' caption='[[3esk]], [[Resolution\|resolution]] 2.05Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19586912}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3esk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ESK FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1elr\|1elr]], [[1elw\|1elw]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3esk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3esk RCSB], [http://www.ebi.ac.uk/pdbsum/3esk PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/STIP1_HUMAN STIP1_HUMAN]] Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). [[http://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN]] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>		[[http://www.uniprot.org/uniprot/STIP1_HUMAN STIP1_HUMAN]] Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). [[http://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN]] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/es/3esk_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Hsp-organizing protein (HOP) binds to the C termini of the chaperones Hsp70 and Hsp90, thus bringing them together so that substrate proteins can be passed from Hsp70 to Hsp90. Because Hsp90 is essential for the correct folding and maturation of many oncogenic proteins, it has become a significant target for anti-cancer drug design. HOP binds to Hsp70 and Hsp90 via two independent tetratricopeptide (TPR) domains, TPR1 and TPR2A, respectively. We have analyzed ligand binding using Poisson-Boltzmann continuum electrostatic calculations, free energy perturbation, molecular dynamics simulations, and site-directed mutagenesis to delineate the contribution of different interactions to the affinity and specificity of the TPR-peptide interactions. We found that continuum electrostatic calculations could be used to guide protein design by removing unfavorable interactions to increase binding affinity, with an 80-fold increase in affinity for TPR2A. Contributions at buried charged residues, however, were better predicted by free energy perturbation calculations. We suggest using a combination of the two approaches for increasing the accuracy of results, with free energy perturbation calculations used only at selected buried residues of the ligand binding pocket. Finally we present the crystal structure of TPR2A in complex with its non-cognate Hsp70 ligand, which provides insight on the origins of specificity in TPR domain-peptide recognition.

-	~~==About this Structure==~~	+	Electrostatic interactions of Hsp-organizing protein tetratricopeptide domains with Hsp70 and Hsp90: computational analysis and protein engineering.,Kajander T, Sachs JN, Goldman A, Regan L J Biol Chem. 2009 Sep 11;284(37):25364-74. Epub 2009 Jul 7. PMID:19586912<ref>PMID:19586912</ref>
-	~~[[3esk]] is a 2 chain structure~~ with ~~sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Human Human]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3ESK OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:019586912</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Human]]		[[Category: Human]]
-	[[Category: Kajander, T.]]	+	[[Category: Kajander, T]]
-	[[Category: Regan, L.]]	+	[[Category: Regan, L]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
	[[Category: Hsc70]]		[[Category: Hsc70]]

OCA at 07:04, 2 April 2014

2014-04-02T07:04:13Z

←Older revision		Revision as of 07:04, 2 April 2014
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-	[[Image:3esk.png\|left\|200px]]
-
	{{STRUCTURE_3esk\| PDB=3esk \| SCENE= }}		{{STRUCTURE_3esk\| PDB=3esk \| SCENE= }}
-
	===Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand===		===Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand===
		+	{{ABSTRACT_PUBMED_19586912}}

-	~~{{ABSTRACT_PUBMED_19586912}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/STIP1_HUMAN STIP1_HUMAN]] Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). [[http://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN]] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>

	==About this Structure==		==About this Structure==
-	[[3esk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA].	+	[[3esk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:019586912</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019586912</ref><references group="xtra"/><references/>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Kajander, T.]]		[[Category: Kajander, T.]]
	[[Category: Regan, L.]]		[[Category: Regan, L.]]

OCA at 13:34, 6 February 2013

2013-02-06T13:34:55Z

←Older revision		Revision as of 13:34, 6 February 2013
Line 1:		Line 1:
	[[Image:3esk.png\|left\|200px]]		[[Image:3esk.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3esk", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3esk\| PDB=3esk \| SCENE= }}		{{STRUCTURE_3esk\| PDB=3esk \| SCENE= }}

	===Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand===		===Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19586912}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19586912 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_19586912}}		{{ABSTRACT_PUBMED_19586912}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19586912~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019586912</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Kajander, T.]]		[[Category: Kajander, T.]]
	[[Category: Regan, L.]]		[[Category: Regan, L.]]
		+	[[Category: Chaperone]]
		+	[[Category: Hsc70]]
		+	[[Category: Hsp90]]
		+	[[Category: Nucleus]]
		+	[[Category: Stress response]]
		+	[[Category: Tetratricopeptide repeat]]
		+	[[Category: Tpr repeat]]
		+	[[Category: Tpr2a]]

OCA: Protected "3esk" [edit=sysop:move=sysop]

2011-01-12T04:37:32Z

Protected "3esk" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:37, 12 January 2011

OCA at 04:37, 12 January 2011

2011-01-12T04:37:31Z

←Older revision		Revision as of 04:37, 12 January 2011
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3esk.png\|left\|200px]]
-	[[Image:3esk.~~jpg~~\|left\|200px]]	+

	<!--		<!--
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	===Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand===		===Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19586912}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19586912 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19586912}}

	==About this Structure==		==About this Structure==
-	~~3ESK~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA].	+	[[3esk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19586912</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Kajander, T.]]		[[Category: Kajander, T.]]
	[[Category: Regan, L.]]		[[Category: Regan, L.]]
-	[[Category: Chaperone]]
-	[[Category: Cytoplasm]]
-	[[Category: Hsc70]]
-	[[Category: Hsp90]]
-	[[Category: Nucleus]]
-	[[Category: Stress response]]
-	[[Category: Tetratricopeptide repeat]]
-	[[Category: Tpr repeat]]
-	[[Category: Tpr2a]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 8 12:40:46 2009''

OCA at 09:40, 8 July 2009

2009-07-08T09:40:49Z

←Older revision		Revision as of 09:40, 8 July 2009
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3esk.jpg\|left\|200px]]

-	The ~~entry 3esk~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3esk", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3esk\| PDB=3esk \| SCENE= }}

-	~~Authors: Kajander, T., Regan, L.~~	+	===Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand===

-	Description: Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Oct 15 14~~:06:~~47 2008~~''	+	==About this Structure==
		+	3ESK is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESK OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Kajander, T.]]
		+	[[Category: Regan, L.]]
		+	[[Category: Chaperone]]
		+	[[Category: Cytoplasm]]
		+	[[Category: Hsc70]]
		+	[[Category: Hsp90]]
		+	[[Category: Nucleus]]
		+	[[Category: Stress response]]
		+	[[Category: Tetratricopeptide repeat]]
		+	[[Category: Tpr repeat]]
		+	[[Category: Tpr2a]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 8 12:40:46 2009''