3fef - Revision history

OCA at 00:31, 28 December 2023

2023-12-28T00:31:27Z

←Older revision		Revision as of 00:31, 28 December 2023
Line 1:		Line 1:

	==Crystal structure of putative glucosidase lplD from bacillus subtilis==		==Crystal structure of putative glucosidase lplD from bacillus subtilis==
-	<StructureSection load='3fef' size='340' side='right'caption='[[3fef]]' scene=''>	+	<StructureSection load='3fef' size='340' side='right'caption='[[3fef]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FEF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fef]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FEF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [https://pdbe.org/3fef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [https://www.ebi.ac.uk/pdbsum/3fef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fef ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [https://pdbe.org/3fef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [https://www.ebi.ac.uk/pdbsum/3fef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fef ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LPLD_BACSU LPLD_BACSU] Alpha-galacturonidase able to catalyze the hydrolysis of the chromogenic substrate p-nitrophenyl-alpha-D-galacturonic acid (pNPalphaGalUA), and of the probable natural substrate alpha-1,4-di-galacturonate (GalUA(2)). Can neither hydrolyze pNPbetaGalUA, nor the stereoisomeric pNPalphaGlcUA. Does not display alpha- or beta-glucosidase activity as it fails to hydrolyze melibiose, raffinose, lactose and the chromogenic analogs, pNPalphaGal and pNPbetaGal. Can not use the following compounds as substrates: pNP-N-acetyl-alpha- and beta-D-galactosaminide, pNP-N-acetyl-alpha- and beta-D-glucosaminide, pNP-alpha-L- and beta-L-arabinopyranoside, pNP-alpha- and beta-D-glucuronide, pNP-alpha- and beta-D-glucopyranoside, pNP-alpha- and beta-D-glucopyranoside 6-phosphate, pNP-alpha-D-galactopyranoside 6-phosphate and oNP-beta-D-galactopyranoside 6-phosphate.<ref>PMID:23416295</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fef ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fef ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Almo SC]]		[[Category: Almo SC]]

OCA at 07:59, 2 March 2022

2022-03-02T07:59:14Z

←Older revision		Revision as of 07:59, 2 March 2022
Line 1:		Line 1:

	==Crystal structure of putative glucosidase lplD from bacillus subtilis==		==Crystal structure of putative glucosidase lplD from bacillus subtilis==
-	<StructureSection load='3fef' size='340' side='right' caption='[[3fef]]~~, [[Resolution\|resolution]] 2.20Å~~' scene=''>	+	<StructureSection load='3fef' size='340' side='right'caption='[[3fef]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[3fef]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FEF FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FEF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [https://pdbe.org/3fef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [https://www.ebi.ac.uk/pdbsum/3fef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fef ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU07130, lplD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galacturan_1,4-alpha-galacturonidase Galacturan 1,4-alpha-galacturonidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.67 3.2.1.67] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [~~http~~://pdbe.org/3fef PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fef PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fef ProSAT], [~~http~~://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[http://www.uniprot.org/uniprot/LPLD_BACSU LPLD_BACSU]] Alpha-galacturonidase able to catalyze the hydrolysis of the chromogenic substrate p-nitrophenyl-alpha-D-galacturonic acid (pNPalphaGalUA), and of the probable natural substrate alpha-1,4-di-galacturonate (GalUA(2)). Can neither hydrolyze pNPbetaGalUA, nor the stereoisomeric pNPalphaGlcUA. Does not display alpha- or beta-glucosidase activity as it fails to hydrolyze melibiose, raffinose, lactose and the chromogenic analogs, pNPalphaGal and pNPbetaGal. Can not use the following compounds as substrates: pNP-N-acetyl-alpha- and beta-D-galactosaminide, pNP-N-acetyl-alpha- and beta-D-glucosaminide, pNP-alpha-L- and beta-L-arabinopyranoside, pNP-alpha- and beta-D-glucuronide, pNP-alpha- and beta-D-glucopyranoside, pNP-alpha- and beta-D-glucopyranoside 6-phosphate, pNP-alpha-D-galactopyranoside 6-phosphate and oNP-beta-D-galactopyranoside 6-phosphate.<ref>PMID:23416295</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/3fef_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/3fef_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 22:		Line 16:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fef ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fef ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio subtilis ehrenberg 1835]]~~	+	[[Category: Large Structures]]
-	~~[[Category: Galacturan 1,4-alpha-galacturonidase~~]]	+	[[Category: Almo SC]]
-	[[Category: Almo~~, S C~~]]	+	[[Category: Burley SK]]
-	[[Category: Burley~~, S K]]~~	+	[[Category: Rajashankar KR]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Ramagopal UA]]
-	[[Category: Rajashankar~~, K R~~]]	+	[[Category: Toro R]]
-	[[Category: Ramagopal~~, U A~~]]	+
-	[[Category: Toro, R]]	+
-	~~[[Category: Glycosidase]]~~	+
-	~~[[Category: Gulosidase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lpld]]~~	+
-	~~[[Category: Manganese]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Nad]]~~	+
-	~~[[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]]~~	+
-	~~[[Category: PSI, Protein structure initiative]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 06:40, 1 November 2017

2017-11-01T06:40:09Z

←Older revision		Revision as of 06:40, 1 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of putative glucosidase lplD from bacillus subtilis==		==Crystal structure of putative glucosidase lplD from bacillus subtilis==
	<StructureSection load='3fef' size='340' side='right' caption='[[3fef]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3fef' size='340' side='right' caption='[[3fef]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fef]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FEF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fef]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FEF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU07130, lplD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "~~Bacillus globigii~~" ~~Migula 1900~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU07130, lplD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galacturan_1,4-alpha-galacturonidase Galacturan 1,4-alpha-galacturonidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.67 3.2.1.67] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galacturan_1,4-alpha-galacturonidase Galacturan 1,4-alpha-galacturonidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.67 3.2.1.67] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [http://pdbe.org/3fef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [http://www.ebi.ac.uk/pdbsum/3fef PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [http://pdbe.org/3fef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [http://www.ebi.ac.uk/pdbsum/3fef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fef ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Galacturan 1,4-alpha-galacturonidase]]		[[Category: Galacturan 1,4-alpha-galacturonidase]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]

OCA at 16:52, 8 February 2016

2016-02-08T16:52:23Z

←Older revision		Revision as of 16:52, 8 February 2016
Line 7:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU07130, lplD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU07130, lplD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galacturan_1,4-alpha-galacturonidase Galacturan 1,4-alpha-galacturonidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.67 3.2.1.67] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galacturan_1,4-alpha-galacturonidase Galacturan 1,4-alpha-galacturonidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.67 3.2.1.67] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [http://www.ebi.ac.uk/pdbsum/3fef PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [http://pdbe.org/3fef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [http://www.ebi.ac.uk/pdbsum/3fef PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fef ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	== References ==		== References ==

OCA at 00:48, 25 December 2014

2014-12-25T00:48:13Z

←Older revision		Revision as of 00:48, 25 December 2014
Line 2:		Line 2:
	<StructureSection load='3fef' size='340' side='right' caption='[[3fef]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3fef' size='340' side='right' caption='[[3fef]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fef]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FEF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fef]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FEF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU07130, lplD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galacturan_1,4-alpha-galacturonidase Galacturan 1,4-alpha-galacturonidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.67 3.2.1.67] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galacturan_1,4-alpha-galacturonidase Galacturan 1,4-alpha-galacturonidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.67 3.2.1.67] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [http://www.ebi.ac.uk/pdbsum/3fef PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [http://www.ebi.ac.uk/pdbsum/3fef PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LPLD_BACSU LPLD_BACSU]] Alpha-galacturonidase able to catalyze the hydrolysis of the chromogenic substrate p-nitrophenyl-alpha-D-galacturonic acid (pNPalphaGalUA), and of the probable natural substrate alpha-1,4-di-galacturonate (GalUA(2)). Can neither hydrolyze pNPbetaGalUA, nor the stereoisomeric pNPalphaGlcUA. Does not display alpha- or beta-glucosidase activity as it fails to hydrolyze melibiose, raffinose, lactose and the chromogenic analogs, pNPalphaGal and pNPbetaGal. Can not use the following compounds as substrates: pNP-N-acetyl-alpha- and beta-D-galactosaminide, pNP-N-acetyl-alpha- and beta-D-glucosaminide, pNP-alpha-L- and beta-L-arabinopyranoside, pNP-alpha- and beta-D-glucuronide, pNP-alpha- and beta-D-glucopyranoside, pNP-alpha- and beta-D-glucopyranoside 6-phosphate, pNP-alpha-D-galactopyranoside 6-phosphate and oNP-beta-D-galactopyranoside 6-phosphate.<ref>PMID:23416295</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 21:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Galacturan 1,4-alpha-galacturonidase]]		[[Category: Galacturan 1,4-alpha-galacturonidase]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]

OCA at 12:43, 6 November 2014

2014-11-06T12:43:29Z

←Older revision		Revision as of 12:43, 6 November 2014
Line 1:		Line 1:
-	~~[[Image:3fef.png\|left\|200px]]~~	+	==Crystal structure of putative glucosidase lplD from bacillus subtilis==
-		+	<StructureSection load='3fef' size='340' side='right' caption='[[3fef]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~{{STRUCTURE_3fef\| PDB=3fef \| SCENE= }}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3fef]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FEF FirstGlance]. <br>
-	===Crystal structure of putative glucosidase lplD from bacillus subtilis===	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galacturan_1,4-alpha-galacturonidase Galacturan 1,4-alpha-galacturonidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.67 3.2.1.67] </span></td></tr>
-	==~~About this Structure~~==	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fef RCSB], [http://www.ebi.ac.uk/pdbsum/3fef PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/3fef TOPSAN]</span></td></tr>
-	[[3fef]] is a 4 chain structure ~~with sequence~~ from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. ~~Full crystallographic information is available from~~ [http://oca.weizmann.ac.il/oca-bin/~~ocashort~~?id=~~3FEF~~ OCA].	+	</table>
-	[[Category: ~~Bacillus subtilis~~]]	+	== Evolutionary Conservation ==
-	[[Category: Almo, S C.]]	+	[[Image:Consurf_key_small.gif\|200px\|right]]
-	[[Category: Burley, S K.]]	+	Check<jmol>
-	[[Category: ~~NYSGXRC, New York SGX Research Center for~~ Structural ~~Genomics.~~]]	+	<jmolCheckbox>
-	[[Category: Rajashankar, K R.]]	+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/3fef_consurf.spt"</scriptWhenChecked>
-	[[Category: Ramagopal, U A.]]	+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-	[[Category: Toro, R.]]	+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Galacturan 1,4-alpha-galacturonidase]]
		+	[[Category: Almo, S C]]
		+	[[Category: Burley, S K]]
		+	[[Category: Structural genomic]]
		+	[[Category: Rajashankar, K R]]
		+	[[Category: Ramagopal, U A]]
		+	[[Category: Toro, R]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
	[[Category: Gulosidase]]		[[Category: Gulosidase]]
Line 22:		Line 34:
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: Nad]]		[[Category: Nad]]
-	[[Category: New ~~york sgx research center~~ for ~~structural genomic]]~~	+	[[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]]
-	~~[[Category: Nysgxrc~~]]	+	[[Category: PSI, Protein structure initiative]]
-	[[Category: Protein structure initiative]]	+
-	~~[[Category: Psi-2]]~~	+
-	~~[[Category: Structural genomic~~]]	+
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA: Protected "3fef" [edit=sysop:move=sysop]

2013-02-13T10:51:25Z

Protected "3fef" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:51, 13 February 2013

OCA at 10:51, 13 February 2013

2013-02-13T10:51:21Z

←Older revision		Revision as of 10:51, 13 February 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3fef.png\|left\|200px]]
-	[[Image:3fef.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3fef", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3fef\| PDB=3fef \| SCENE= }}		{{STRUCTURE_3fef\| PDB=3fef \| SCENE= }}

Line 14:		Line 7:

	==About this Structure==		==About this Structure==
-	~~3FEF~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA].	+	[[3fef]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA].
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Almo, S C.]]		[[Category: Almo, S C.]]
Line 35:		Line 28:
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Unknown function]]		[[Category: Unknown function]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 31 10:38:44 2008''

OCA at 08:38, 31 December 2008

2008-12-31T08:38:47Z

←Older revision		Revision as of 08:38, 31 December 2008
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3fef.jpg\|left\|200px]]

-	The ~~entry 3fef~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3fef", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3fef\| PDB=3fef \| SCENE= }}

-	~~Authors: Ramagopal, U.A., Rajashankar, K.R., Toro, R., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomics (NYSGXRC)~~	+	===Crystal structure of putative glucosidase lplD from bacillus subtilis===

-	Description: Crystal structure of putative glucosidase lplD from bacillus subtilis

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 14:42:42 2008''	+	==About this Structure==
		+	3FEF is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FEF OCA].
		+	[[Category: Bacillus subtilis]]
		+	[[Category: Almo, S C.]]
		+	[[Category: Burley, S K.]]
		+	[[Category: NYSGXRC, New York SGX Research Center for Structural Genomics.]]
		+	[[Category: Rajashankar, K R.]]
		+	[[Category: Ramagopal, U A.]]
		+	[[Category: Toro, R.]]
		+	[[Category: Glycosidase]]
		+	[[Category: Gulosidase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lpld]]
		+	[[Category: Manganese]]
		+	[[Category: Metal-binding]]
		+	[[Category: Nad]]
		+	[[Category: New york sgx research center for structural genomic]]
		+	[[Category: Nysgxrc]]
		+	[[Category: Protein structure initiative]]
		+	[[Category: Psi-2]]
		+	[[Category: Structural genomic]]
		+	[[Category: Unknown function]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 31 10:38:44 2008''

OCA: New page: '''Unreleased structure''' The entry 3fef is ON HOLD Authors: Ramagopal, U.A., Rajashankar, K.R., Toro, R., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomic...

2008-12-10T12:42:43Z

New page: '''Unreleased structure''' The entry 3fef is ON HOLD Authors: Ramagopal, U.A., Rajashankar, K.R., Toro, R., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomic...

New page

'''Unreleased structure'''

The entry 3fef is ON HOLD

Authors: Ramagopal, U.A., Rajashankar, K.R., Toro, R., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomics (NYSGXRC)

Description: Crystal structure of putative glucosidase lplD from bacillus subtilis

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 14:42:42 2008''