3fil - Revision history

OCA at 15:29, 1 November 2023

2023-11-01T15:29:50Z

←Older revision		Revision as of 15:29, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3fil' size='340' side='right'caption='[[3fil]], [[Resolution\|resolution]] 0.88Å' scene=''>		<StructureSection load='3fil' size='340' side='right'caption='[[3fil]], [[Resolution\|resolution]] 0.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fil]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Strsg Strsg~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FIL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fil]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FIL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.88Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~FME~~:~~N-FORMYLMETHIONINE~~'>~~FME~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;~~'>~~[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]~~</~~div~~>~~</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 STRSG])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [https://pdbe.org/3fil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [https://www.ebi.ac.uk/pdbsum/3fil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fil ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [https://pdbe.org/3fil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [https://www.ebi.ac.uk/pdbsum/3fil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fil ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPG2_STRSG SPG2_STRSG]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Strsg~~]]	+	[[Category: Streptococcus sp. 'group G']]
-	[[Category: Heinemann, U]]	+	[[Category: Heinemann U]]
-	[[Category: Max~~, K E.A]]~~	+	[[Category: Max KEA]]
-	~~[[Category: Alpha helix]]~~	+
-	~~[[Category: Beta sheet]]~~	+
-	~~[[Category: Cell wall]]~~	+
-	~~[[Category: Dimerization]]~~	+
-	~~[[Category: Igg-binding protein]]~~	+
-	~~[[Category: Improved hydrophobic packing of core residue]]~~	+
-	~~[[Category: Peptidoglycan-anchor]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Secreted~~]]	+

OCA at 07:39, 10 November 2021

2021-11-10T07:39:31Z

←Older revision		Revision as of 07:39, 10 November 2021
Line 1:		Line 1:

	==Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution==		==Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution==
-	<StructureSection load='3fil' size='340' side='right' caption='[[3fil]], [[Resolution\|resolution]] 0.88Å' scene=''>	+	<StructureSection load='3fil' size='340' side='right'caption='[[3fil]], [[Resolution\|resolution]] 0.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fil]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Strsg Strsg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FIL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fil]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Strsg Strsg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FIL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 STRSG])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 STRSG])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [~~http~~://pdbe.org/3fil PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fil PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fil ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [https://pdbe.org/3fil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [https://www.ebi.ac.uk/pdbsum/3fil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fil ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/3fil_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/3fil_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 3fil" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3fil" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Protein G\|Protein G]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Strsg]]		[[Category: Strsg]]
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]

OCA at 06:43, 1 November 2017

2017-11-01T06:43:17Z

←Older revision		Revision as of 06:43, 1 November 2017
Line 1:		Line 1:
		+
	==Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution==		==Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution==
	<StructureSection load='3fil' size='340' side='right' caption='[[3fil]], [[Resolution\|resolution]] 0.88Å' scene=''>		<StructureSection load='3fil' size='340' side='right' caption='[[3fil]], [[Resolution\|resolution]] 0.88Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 STRSG])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 STRSG])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [http://pdbe.org/3fil PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [http://www.ebi.ac.uk/pdbsum/3fil PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [http://pdbe.org/3fil PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [http://www.ebi.ac.uk/pdbsum/3fil PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fil ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 3fil" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3fil" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Protein G\|Protein G]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:44, 10 February 2016

2016-02-10T04:44:22Z

←Older revision		Revision as of 04:44, 10 February 2016
Line 2:		Line 2:
	<StructureSection load='3fil' size='340' side='right' caption='[[3fil]], [[Resolution\|resolution]] 0.88Å' scene=''>		<StructureSection load='3fil' size='340' side='right' caption='[[3fil]], [[Resolution\|resolution]] 0.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fil]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_sp._'group_g' Streptococcus sp. 'group g'~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FIL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fil]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strsg Strsg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FIL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 ~~Streptococcus sp. 'group G'~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 STRSG])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [http://www.ebi.ac.uk/pdbsum/3fil PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [http://pdbe.org/3fil PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [http://www.ebi.ac.uk/pdbsum/3fil PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fil ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3fil" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptococcus sp. 'group g'~~]]	+	[[Category: Strsg]]
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]
	[[Category: Max, K E.A]]		[[Category: Max, K E.A]]

OCA at 22:56, 3 January 2015

2015-01-03T22:56:40Z

←Older revision		Revision as of 22:56, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3fil]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._'group_g' Streptococcus sp. 'group g']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FIL FirstGlance]. <br>		<table><tr><td colspan='2'>[[3fil]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._'group_g' Streptococcus sp. 'group g']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FIL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 Streptococcus sp. 'group G'])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 Streptococcus sp. 'group G'])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [http://www.ebi.ac.uk/pdbsum/3fil PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [http://www.ebi.ac.uk/pdbsum/3fil PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 25:
	Dimer formation of a stabilized Gbeta1 variant: a structural and energetic analysis.,Thoms S, Max KE, Wunderlich M, Jacso T, Lilie H, Reif B, Heinemann U, Schmid FX J Mol Biol. 2009 Sep 4;391(5):918-32. Epub 2009 Jun 13. PMID:19527728<ref>PMID:19527728</ref>		Dimer formation of a stabilized Gbeta1 variant: a structural and energetic analysis.,Thoms S, Max KE, Wunderlich M, Jacso T, Lilie H, Reif B, Heinemann U, Schmid FX J Mol Biol. 2009 Sep 4;391(5):918-32. Epub 2009 Jun 13. PMID:19527728<ref>PMID:19527728</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Protein G\|Protein G]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Streptococcus sp. 'group g']]		[[Category: Streptococcus sp. 'group g']]
-	[[Category: Heinemann, U.]]	+	[[Category: Heinemann, U]]
-	[[Category: Max, K E.A.]]	+	[[Category: Max, K E.A]]
	[[Category: Alpha helix]]		[[Category: Alpha helix]]
	[[Category: Beta sheet]]		[[Category: Beta sheet]]

OCA: Protected "3fil" [edit=sysop:move=sysop]

2014-05-21T09:51:39Z

Protected "3fil" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:51, 21 May 2014

OCA at 09:51, 21 May 2014

2014-05-21T09:51:35Z

←Older revision		Revision as of 09:51, 21 May 2014
Line 1:		Line 1:
-	~~{{Seed}}~~	+	==Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution==
-	[[~~Image:~~3fil.~~png~~\|~~left~~\|200px]]	+	<StructureSection load='3fil' size='340' side='right' caption='[[3fil]], [[Resolution\|resolution]] 0.88Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3fil]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._'group_g' Streptococcus sp. 'group g']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FIL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2on8\|2on8]], [[2onq\|2onq]], [[1pga\|1pga]], [[1gb4\|1gb4]], [[1fcc\|1fcc]], [[2qmt\|2qmt]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 Streptococcus sp. 'group G'])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fil RCSB], [http://www.ebi.ac.uk/pdbsum/3fil PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/3fil_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In previous work, a strongly stabilized variant of the beta1 domain of streptococcal protein G (Gbeta1) was obtained by an in vitro selection method. This variant, termed Gbeta1-M2, contains the four substitutions E15V, T16L, T18I, and N37L. Here we elucidated the molecular basis of the observed strong stabilizations. The contributions of these four residues were analyzed individually and in various combinations, additional selections with focused Gbeta1 gene libraries were performed, and the crystal structure of Gbeta1-M2 was determined. All single substitutions (E15V, T16L, T18I, and N37L) stabilize wild-type Gbeta1 by contributions of between 1.6 and 6.0 kJ mol(-1) (at 70 degrees C). Hydrophobic residues at positions 16 and 37 provide the major contribution to stabilization by enlarging the hydrophobic core of Gbeta1. They also increase the tendency to form dimers, as shown by dependence on the concentration of apparent molecular mass in analytical ultracentrifugation, by concentration-dependent stability, and by a strongly increased van't Hoff enthalpy of unfolding. The 0.88-A crystal structure of Gbeta1-M2 and NMR measurements in solution provide the explanation for the observed dimer formation. It involves a head-to-head arrangement of two Gbeta1-M2 molecules via six intermolecular hydrogen bonds between the two beta strands 2 and 2' and an adjacent self-complementary hydrophobic surface area, which is created by the T16L and N37L substitutions and a large 120 degrees rotation of the Tyr33 side chain. This removal of hydrophilic groups and the malleability of the created hydrophobic surface provide the basis for the dimer formation of stabilized Gbeta1 variants.

-	~~<!--~~	+	Dimer formation of a stabilized Gbeta1 variant: a structural and energetic analysis.,Thoms S, Max KE, Wunderlich M, Jacso T, Lilie H, Reif B, Heinemann U, Schmid FX J Mol Biol. 2009 Sep 4;391(5):918-32. Epub 2009 Jun 13. PMID:19527728<ref>PMID:19527728</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3fil"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3fil\| PDB=3fil \| SCENE= }}~~	+

-	~~===Structural and energetic determinants for hyperstable variants~~ of ~~GB1 obtained from in-vitro evolution===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_19527728}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 19527728 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+	[[Category: Streptococcus sp. 'group g']]
-	~~{{ABSTRACT_PUBMED_19527728}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~3FIL is a 2 chains structure of sequences from [http:~~/~~/en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:19527728<~~/~~ref~~><~~references group="xtra"~~/>	+
-	[[Category: ~~Bacteria~~]]	+
	[[Category: Heinemann, U.]]		[[Category: Heinemann, U.]]
	[[Category: Max, K E.A.]]		[[Category: Max, K E.A.]]
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	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Secreted]]		[[Category: Secreted]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 16:30:24 2009''

OCA at 13:30, 3 September 2009

2009-09-03T13:30:26Z

←Older revision		Revision as of 13:30, 3 September 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3fil.~~jpg~~\|left\|200px]]	+	[[Image:3fil.png\|left\|200px]]

	<!--		<!--
Line 37:		Line 37:
	[[Category: Secreted]]		[[Category: Secreted]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Aug 19 13~~:11:58 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 16:30:24 2009''

OCA at 10:12, 19 August 2009

2009-08-19T10:12:03Z

←Older revision		Revision as of 10:12, 19 August 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3fil.jpg\|left\|200px]]

-	The ~~entry 3fil~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3fil", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3fil\| PDB=3fil \| SCENE= }}

-	~~Authors: Max, K.E.A., Heinemann, U.~~	+	===Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution===

-	Description: Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 14 12~~:54:19 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19527728}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19527728 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19527728}}
		+
		+	==About this Structure==
		+	3FIL is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIL OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19527728</ref><references group="xtra"/>
		+	[[Category: Bacteria]]
		+	[[Category: Heinemann, U.]]
		+	[[Category: Max, K E.A.]]
		+	[[Category: Alpha helix]]
		+	[[Category: Beta sheet]]
		+	[[Category: Cell wall]]
		+	[[Category: Dimerization]]
		+	[[Category: Igg-binding protein]]
		+	[[Category: Improved hydrophobic packing of core residue]]
		+	[[Category: Peptidoglycan-anchor]]
		+	[[Category: Protein binding]]
		+	[[Category: Secreted]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 19 13:11:58 2009''

OCA at 10:54, 14 January 2009

2009-01-14T10:54:19Z

←Older revision		Revision as of 10:54, 14 January 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3fil is ON HOLD	+	The entry 3fil is ON HOLD until Paper Publication

	Authors: Max, K.E.A., Heinemann, U.		Authors: Max, K.E.A., Heinemann, U.
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	Description: Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution		Description: Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Dec 31 09~~:48:19 ~~2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 14 12:54:19 2009''