3fk7 - Revision history

OCA at 15:30, 1 November 2023

2023-11-01T15:30:09Z

←Older revision		Revision as of 15:30, 1 November 2023
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	<StructureSection load='3fk7' size='340' side='right'caption='[[3fk7]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='3fk7' size='340' side='right'caption='[[3fk7]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FK7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FK7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.06Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vke\|2vke]], [[2o7o\|2o7o]], [[2vkv\|2vkv]], [[2tct\|2tct]], [[3fk6\|3fk6]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tetR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [https://pdbe.org/3fk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [https://www.ebi.ac.uk/pdbsum/3fk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fk7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [https://pdbe.org/3fk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [https://www.ebi.ac.uk/pdbsum/3fk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fk7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.	+	[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.[https://www.uniprot.org/uniprot/TETR4_ECOLX TETR4_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gmeiner, P]]	+	[[Category: Gmeiner P]]
-	[[Category: Hillen, W]]	+	[[Category: Hillen W]]
-	[[Category: Klieber~~, M A~~]]	+	[[Category: Klieber MA]]
-	[[Category: Lochner, S]]	+	[[Category: Lochner S]]
-	[[Category: Muller~~, Y A~~]]	+	[[Category: Muller YA]]
-	[[Category: Scholz, O]]	+	[[Category: Scholz O]]
-	~~[[Category: 4-de-dimethylamino-anhydrotetracycline]]~~	+
-	~~[[Category: Altered inducer specificity]]~~	+
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Bacterial transcription regulation]]~~	+
-	~~[[Category: Dna-binding]]~~	+
-	~~[[Category: Magnesium]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Repressor]]~~	+
-	~~[[Category: Tetracycline repressor]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Transposable element~~]]	+

OCA at 07:39, 10 November 2021

2021-11-10T07:39:41Z

←Older revision		Revision as of 07:39, 10 November 2021
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	==Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc==		==Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc==
-	<StructureSection load='3fk7' size='340' side='right' caption='[[3fk7]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='3fk7' size='340' side='right'caption='[[3fk7]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FK7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FK7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vke\|2vke]], [[2o7o\|2o7o]], [[2vkv\|2vkv]], [[2tct\|2tct]], [[3fk6\|3fk6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vke\|2vke]], [[2o7o\|2o7o]], [[2vkv\|2vkv]], [[2tct\|2tct]], [[3fk6\|3fk6]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tetR ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tetR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [~~http~~://pdbe.org/3fk7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fk7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fk7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [https://pdbe.org/3fk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [https://www.ebi.ac.uk/pdbsum/3fk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fk7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.	+	[[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/3fk7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/3fk7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 3fk7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3fk7" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Gmeiner, P]]		[[Category: Gmeiner, P]]
	[[Category: Hillen, W]]		[[Category: Hillen, W]]

OCA at 06:43, 1 November 2017

2017-11-01T06:43:50Z

←Older revision		Revision as of 06:43, 1 November 2017
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	</div>		</div>
	<div class="pdbe-citations 3fk7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3fk7" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Tetracycline repressor protein\|Tetracycline repressor protein]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
		+	[[Category: Transposable element]]

OCA at 15:46, 5 August 2016

2016-08-05T15:46:47Z

←Older revision		Revision as of 15:46, 5 August 2016
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		+
	==Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc==		==Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc==
	<StructureSection load='3fk7' size='340' side='right' caption='[[3fk7]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='3fk7' size='340' side='right' caption='[[3fk7]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FK7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FK7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vke\|2vke]], [[2o7o\|2o7o]], [[2vkv\|2vkv]], [[2tct\|2tct]], [[3fk6\|3fk6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vke\|2vke]], [[2o7o\|2o7o]], [[2vkv\|2vkv]], [[2tct\|2tct]], [[3fk6\|3fk6]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tetR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tetR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3fk7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [http://pdbe.org/3fk7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3fk7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fk7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fk7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3fk7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Gmeiner, P]]		[[Category: Gmeiner, P]]
	[[Category: Hillen, W]]		[[Category: Hillen, W]]

OCA at 20:01, 25 December 2014

2014-12-25T20:01:41Z

←Older revision		Revision as of 20:01, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FK7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FK7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vke\|2vke]], [[2o7o\|2o7o]], [[2vkv\|2vkv]], [[2tct\|2tct]], [[3fk6\|3fk6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vke\|2vke]], [[2o7o\|2o7o]], [[2vkv\|2vkv]], [[2tct\|2tct]], [[3fk6\|3fk6]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tetR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tetR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3fk7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3fk7 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Gmeiner, P.]]	+	[[Category: Gmeiner, P]]
-	[[Category: Hillen, W.]]	+	[[Category: Hillen, W]]
-	[[Category: Klieber, M A.]]	+	[[Category: Klieber, M A]]
-	[[Category: Lochner, S.]]	+	[[Category: Lochner, S]]
-	[[Category: Muller, Y A.]]	+	[[Category: Muller, Y A]]
-	[[Category: Scholz, O.]]	+	[[Category: Scholz, O]]
	[[Category: 4-de-dimethylamino-anhydrotetracycline]]		[[Category: 4-de-dimethylamino-anhydrotetracycline]]
	[[Category: Altered inducer specificity]]		[[Category: Altered inducer specificity]]

OCA at 12:12, 29 September 2014

2014-09-29T12:12:04Z

←Older revision		Revision as of 12:12, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3fk7.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc==
		+	<StructureSection load='3fk7' size='340' side='right' caption='[[3fk7]], [[Resolution\|resolution]] 2.06Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FK7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vke\|2vke]], [[2o7o\|2o7o]], [[2vkv\|2vkv]], [[2tct\|2tct]], [[3fk6\|3fk6]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tetR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3fk7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/3fk7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterial tetracycline transcription regulation system mediated by the tetracycline repressor (TetR) is widely used to study gene expression in prokaryotes and eukaryotes. To study multiple genes in parallel, a triple mutant TetR(K(64)L(135)I(138)) has been engineered that is selectively induced by the synthetic tetracycline derivative 4-de-dimethylamino-anhydrotetracycline (4-ddma-atc) and no longer by tetracycline, the inducer of wild-type TetR. In the present study, we report the crystal structure of TetR(K(64)L(135)I(138)) in the absence and in complex with 4-ddma-atc at resolutions of 2.1 A. Analysis of the structures in light of the available binding data and previously reported TetR complexes allows for a dissection of the origins of selectivity and specificity. In all crystal structures solved to date, the ligand-binding position, as well as the positioning of the residues lining the binding site, is extremely well conserved, irrespective of the chemical nature of the ligand. Selective recognition of 4-ddma-atc is achieved through fine-tuned hydrogen-bonding constraints introduced by the His64-->Lys substitution, as well as a combination of hydrophobic effect and the removal of unfavorable electrostatic interactions through the introduction of Leu135 and Ile138.

-	~~{{STRUCTURE_3fk7\| PDB=3fk7 \| SCENE= }}~~	+	Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair.,Klieber MA, Scholz O, Lochner S, Gmeiner P, Hillen W, Muller YA FEBS J. 2009 Oct;276(19):5610-21. Epub 2009 Aug 27. PMID:19712110<ref>PMID:19712110</ref>

-	~~===Crystal structure of TetR triple mutant (H64K~~, ~~S135L, S138I) in complex with 4-ddma-atc===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19712110}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3fk7]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA].~~	+

	==See Also==		==See Also==
	*[[Tetracycline repressor protein\|Tetracycline repressor protein]]		*[[Tetracycline repressor protein\|Tetracycline repressor protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019712110<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Gmeiner, P.]]		[[Category: Gmeiner, P.]]

OCA at 20:52, 20 October 2012

2012-10-20T20:52:40Z

←Older revision		Revision as of 20:52, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3fk7]] is a 2 chain structure ~~of [[Tetracycline repressor protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA].	+	[[3fk7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA].

	==See Also==		==See Also==

OCA: Protected "3fk7" [edit=sysop:move=sysop]

2012-07-27T11:54:27Z

Protected "3fk7" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:54, 27 July 2012

OCA at 11:54, 27 July 2012

2012-07-27T11:54:25Z

←Older revision		Revision as of 11:54, 27 July 2012
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-	{{Seed}}
	[[Image:3fk7.png\|left\|200px]]		[[Image:3fk7.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3fk7", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3fk7\| PDB=3fk7 \| SCENE= }}		{{STRUCTURE_3fk7\| PDB=3fk7 \| SCENE= }}

	===Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc===		===Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19712110}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19712110 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_19712110}}		{{ABSTRACT_PUBMED_19712110}}

	==About this Structure==		==About this Structure==
-	~~3FK7~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA].	+	[[3fk7]] is a 2 chain structure of [[Tetracycline repressor protein]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA].
		+
		+	==See Also==
		+	*[[Tetracycline repressor protein\|Tetracycline repressor protein]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19712110~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019712110</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Gmeiner, P.]]		[[Category: Gmeiner, P.]]
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	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
-	[[Category: Transposable element]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 28 14:36:48 2010''

OCA at 12:36, 28 January 2010

2010-01-28T12:36:49Z

←Older revision		Revision as of 12:36, 28 January 2010
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	{{Seed}}		{{Seed}}
-	[[Image:3fk7.~~jpg~~\|left\|200px]]	+	[[Image:3fk7.png\|left\|200px]]

	<!--		<!--
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	==About this Structure==		==About this Structure==
-	3FK7 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA].	+	3FK7 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA].

	==Reference==		==Reference==
Line 44:		Line 44:
	[[Category: Transposable element]]		[[Category: Transposable element]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Nov 4 10~~:55:~~50 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 28 14:36:48 2010''