3fot - Revision history

OCA at 09:50, 21 February 2024

2024-02-21T09:50:11Z

←Older revision		Revision as of 09:50, 21 February 2024
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	<StructureSection load='3fot' size='340' side='right'caption='[[3fot]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3fot' size='340' side='right'caption='[[3fot]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Fussp Fussp~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FOT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FOT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TRI3 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=5514 FUSSP])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fot OCA], [https://pdbe.org/3fot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fot RCSB], [https://www.ebi.ac.uk/pdbsum/3fot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fot ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fot OCA], [https://pdbe.org/3fot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fot RCSB], [https://www.ebi.ac.uk/pdbsum/3fot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fot ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRI3_FUSSP TRI3_FUSSP] 15-O-acetyltransferase; part of the core gene cluster that mediates the biosynthesis of trichothecenes, a very large family of chemically related bicyclic sesquiterpene compounds acting as mycotoxins, including T2-toxin (PubMed:8593041, PubMed:19319932). The biosynthesis of trichothecenes begins with the cyclization of farnesyl diphosphate to trichodiene and is catalyzed by the trichodiene synthase TRI5 (PubMed:3800398). Trichodiene undergoes a series of oxygenations catalyzed by the cytochrome P450 monooxygenase TRI4 (PubMed:7651333). TRI4 controls the addition of four oxygens at C-2, C-3, C-11, and the C-12, C-13-epoxide to form the intermediate isotrichotriol (PubMed:16917519). Isotrichotriol then undergoes a non-enzymatic isomerization and cyclization to form isotrichodermol (PubMed:2317042). During this process, the oxygen at the C-2 position becomes the pyran ring oxygen and the hydroxyl group at C-11 is lost (PubMed:2317042). More complex type A trichothecenes are built by modifying isotrichodermol through a series of paired hydroxylation and acetylation or acylation steps (PubMed:11352533). Isotrichodermol is converted to isotrichodermin by the acetyltransferase TRI101 (PubMed:10583973). TRI101 encodes a C-3 transacetylase that acts as a self-protection or resistance factor during biosynthesis and that the presence of a free C-3 hydroxyl group is a key component of Fusarium trichothecene phytotoxicity (PubMed:10583973). A second hydroxyl group is added to C-15 by the trichothecene C-15 hydroxylase TRI11, producing 15-decalonectrin, which is then acetylated by TRI3, producing calonectrin (PubMed:9435078, PubMed:8593041, PubMed:19319932). A third hydroxyl group is added at C-4 by the cytochrome P450 monooxygenase TRI13, converting calonectrin to 3,15-diacetoxyspirpenol, which is subsequently acetylated by the acetyltransferase TRI7 (PubMed:12135578, PubMed:11352533). A fourth hydroxyl group is added to C-8 by the cytochrome P450 monooxygenase TRI1, followed by the addition of an isovaleryl moiety by TRI16 (PubMed:12620849, PubMed:14532047). Finally, the acetyl group is removed from the C-3 position by the trichothecene C-3 esterase TRI8 to produce T-2 toxin (PubMed:12039755).<ref>PMID:10583973</ref> <ref>PMID:11352533</ref> <ref>PMID:12039755</ref> <ref>PMID:12135578</ref> <ref>PMID:12620849</ref> <ref>PMID:14532047</ref> <ref>PMID:16917519</ref> <ref>PMID:19319932</ref> <ref>PMID:2317042</ref> <ref>PMID:3800398</ref> <ref>PMID:7651333</ref> <ref>PMID:8593041</ref> <ref>PMID:9435078</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fot ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fot ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Fusarium head blight is a devastating disease of cereal crops whose worldwide incidence is increasing and at present there is no satisfactory way of combating this pathogen or its associated toxins. There is a wide variety of trichothecene mycotoxins and they all contain a 12,13-epoxytrichothecene skeleton but differ in their substitutions. Indeed, there is considerable variation in the toxin profile across the numerous Fusarium species that has been ascribed to differences in the presence or absence of biosynthetic enzymes and their relative activity. This article addresses the source of differences in acetylation at the C15 position of the trichothecene molecule. Here, we present the in vitro structural and biochemical characterization of TRI3, a 15-O-trichothecene acetyltransferase isolated from F. sporotrichioides and the "in vivo" characterization of Deltatri3 mutants of deoxynivalenol (DON) producing F. graminearum strains. A kinetic analysis shows that TRI3 is an efficient enzyme with the native substrate, 15-decalonectrin, but is inactive with either DON or nivalenol. The structure of TRI3 complexed with 15-decalonectrin provides an explanation for this specificity and shows that Tri3 and Tri101 (3-O-trichothecene acetyltransferase) are evolutionarily related. The active site residues are conserved across all sequences for TRI3 orthologs, suggesting that differences in acetylation at C15 are not due to differences in Tri3. The tri3 deletion mutant shows that acetylation at C15 is required for DON biosynthesis even though DON lacks a C15 acetyl group. The enzyme(s) responsible for deacetylation at the 15 position of the trichothecene mycotoxins have not been identified.
-
-	Structural and functional characterization of TRI3 trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides.,Garvey GS, McCormick SP, Alexander NJ, Rayment I Protein Sci. 2009 Apr;18(4):747-61. PMID:19319932<ref>PMID:19319932</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3fot" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Fussp~~]]	+	[[Category: Fusarium sporotrichioides]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alexander~~, N J~~]]	+	[[Category: Alexander NJ]]
-	[[Category: Garvey~~, G S~~]]	+	[[Category: Garvey GS]]
-	[[Category: McCormick~~, S P~~]]	+	[[Category: McCormick SP]]
-	[[Category: Rayment, I]]	+	[[Category: Rayment I]]
-	~~[[Category: Acetyltransferase]]~~	+
-	~~[[Category: Bahd superfamily]]~~	+
-	~~[[Category: Coenzyme some]]~~	+
-	~~[[Category: Deoxynivalenol]]~~	+
-	~~[[Category: Fusarium graminearum]]~~	+
-	~~[[Category: Fusarium head blight]]~~	+
-	~~[[Category: Fusarium sporotrichioide]]~~	+
-	~~[[Category: T-2 toxin]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Trichothecene mycotoxin~~]]	+

OCA at 08:06, 2 March 2022

2022-03-02T08:06:55Z

←Older revision		Revision as of 08:06, 2 March 2022
Line 1:		Line 1:

	==Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides==		==Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides==
-	<StructureSection load='3fot' size='340' side='right' caption='[[3fot]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='3fot' size='340' side='right'caption='[[3fot]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fot]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Fussp Fussp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FOT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fussp Fussp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FOT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TRI3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 FUSSP])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TRI3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 FUSSP])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fot OCA], [~~http~~://pdbe.org/3fot PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fot RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fot PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fot ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fot OCA], [https://pdbe.org/3fot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fot RCSB], [https://www.ebi.ac.uk/pdbsum/3fot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fot ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</StructureSection>		</StructureSection>
	[[Category: Fussp]]		[[Category: Fussp]]
		+	[[Category: Large Structures]]
	[[Category: Alexander, N J]]		[[Category: Alexander, N J]]
	[[Category: Garvey, G S]]		[[Category: Garvey, G S]]

OCA at 07:59, 5 December 2018

2018-12-05T07:59:57Z

←Older revision		Revision as of 07:59, 5 December 2018
Line 1:		Line 1:
		+
	==Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides==		==Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides==
	<StructureSection load='3fot' size='340' side='right' caption='[[3fot]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3fot' size='340' side='right' caption='[[3fot]], [[Resolution\|resolution]] 1.75Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TRI3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 FUSSP])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TRI3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 FUSSP])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fot OCA], [http://pdbe.org/3fot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fot RCSB], [http://www.ebi.ac.uk/pdbsum/3fot PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fot OCA], [http://pdbe.org/3fot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fot RCSB], [http://www.ebi.ac.uk/pdbsum/3fot PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fot ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/3fot_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/3fot_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:46, 8 February 2016

2016-02-08T20:46:41Z

←Older revision		Revision as of 20:46, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3fot' size='340' side='right' caption='[[3fot]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3fot' size='340' side='right' caption='[[3fot]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Fusarium_sporotrichioides Fusarium sporotrichioides~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FOT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fussp Fussp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FOT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TRI3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 ~~Fusarium sporotrichioides~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TRI3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 FUSSP])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fot RCSB], [http://www.ebi.ac.uk/pdbsum/3fot PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fot OCA], [http://pdbe.org/3fot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fot RCSB], [http://www.ebi.ac.uk/pdbsum/3fot PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fot ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3fot" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Fusarium sporotrichioides~~]]	+	[[Category: Fussp]]
	[[Category: Alexander, N J]]		[[Category: Alexander, N J]]
	[[Category: Garvey, G S]]		[[Category: Garvey, G S]]

OCA at 10:20, 3 December 2014

2014-12-03T10:20:40Z

←Older revision		Revision as of 10:20, 3 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3fot.~~png~~\|~~left~~\|200px]]	+	==Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides==
		+	<StructureSection load='3fot' size='340' side='right' caption='[[3fot]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3fot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FOT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TRI3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 Fusarium sporotrichioides])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fot RCSB], [http://www.ebi.ac.uk/pdbsum/3fot PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/3fot_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fusarium head blight is a devastating disease of cereal crops whose worldwide incidence is increasing and at present there is no satisfactory way of combating this pathogen or its associated toxins. There is a wide variety of trichothecene mycotoxins and they all contain a 12,13-epoxytrichothecene skeleton but differ in their substitutions. Indeed, there is considerable variation in the toxin profile across the numerous Fusarium species that has been ascribed to differences in the presence or absence of biosynthetic enzymes and their relative activity. This article addresses the source of differences in acetylation at the C15 position of the trichothecene molecule. Here, we present the in vitro structural and biochemical characterization of TRI3, a 15-O-trichothecene acetyltransferase isolated from F. sporotrichioides and the "in vivo" characterization of Deltatri3 mutants of deoxynivalenol (DON) producing F. graminearum strains. A kinetic analysis shows that TRI3 is an efficient enzyme with the native substrate, 15-decalonectrin, but is inactive with either DON or nivalenol. The structure of TRI3 complexed with 15-decalonectrin provides an explanation for this specificity and shows that Tri3 and Tri101 (3-O-trichothecene acetyltransferase) are evolutionarily related. The active site residues are conserved across all sequences for TRI3 orthologs, suggesting that differences in acetylation at C15 are not due to differences in Tri3. The tri3 deletion mutant shows that acetylation at C15 is required for DON biosynthesis even though DON lacks a C15 acetyl group. The enzyme(s) responsible for deacetylation at the 15 position of the trichothecene mycotoxins have not been identified.

-	~~{{STRUCTURE_3fot\| PDB=3fot \| SCENE= }}~~	+	Structural and functional characterization of TRI3 trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides.,Garvey GS, McCormick SP, Alexander NJ, Rayment I Protein Sci. 2009 Apr;18(4):747-61. PMID:19319932<ref>PMID:19319932</ref>

-	~~===Structural and Functional Characterization~~ of ~~TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19319932}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3fot]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019319932<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Fusarium sporotrichioides]]		[[Category: Fusarium sporotrichioides]]
-	[[Category: Alexander, N J.]]	+	[[Category: Alexander, N J]]
-	[[Category: Garvey, G S.]]	+	[[Category: Garvey, G S]]
-	[[Category: McCormick, S P.]]	+	[[Category: McCormick, S P]]
-	[[Category: Rayment, I.]]	+	[[Category: Rayment, I]]
	[[Category: Acetyltransferase]]		[[Category: Acetyltransferase]]
	[[Category: Bahd superfamily]]		[[Category: Bahd superfamily]]

OCA: Protected "3fot" [edit=sysop:move=sysop]

2013-02-20T14:37:47Z

Protected "3fot" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:37, 20 February 2013

OCA at 14:37, 20 February 2013

2013-02-20T14:37:44Z

←Older revision		Revision as of 14:37, 20 February 2013
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-	{{Seed}}
	[[Image:3fot.png\|left\|200px]]		[[Image:3fot.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3fot", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3fot\| PDB=3fot \| SCENE= }}		{{STRUCTURE_3fot\| PDB=3fot \| SCENE= }}

	===Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides===		===Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19319932}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19319932 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19319932}}		{{ABSTRACT_PUBMED_19319932}}

	==About this Structure==		==About this Structure==
-	~~3FOT~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA].	+	[[3fot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19319932~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019319932</ref><references group="xtra"/>
	[[Category: Fusarium sporotrichioides]]		[[Category: Fusarium sporotrichioides]]
	[[Category: Alexander, N J.]]		[[Category: Alexander, N J.]]
Line 39:		Line 27:
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Trichothecene mycotoxin]]		[[Category: Trichothecene mycotoxin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 13 10:00:23 2009''

OCA at 07:00, 13 May 2009

2009-05-13T07:00:25Z

←Older revision		Revision as of 07:00, 13 May 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3fot.~~jpg~~\|left\|200px]]	+	[[Image:3fot.png\|left\|200px]]

	<!--		<!--
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	===Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides===		===Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19319932}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19319932 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19319932}}

	==About this Structure==		==About this Structure==
	3FOT is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA].		3FOT is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19319932</ref><references group="xtra"/>
	[[Category: Fusarium sporotrichioides]]		[[Category: Fusarium sporotrichioides]]
	[[Category: Alexander, N J.]]		[[Category: Alexander, N J.]]
Line 31:		Line 40:
	[[Category: Trichothecene mycotoxin]]		[[Category: Trichothecene mycotoxin]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May ~~6 11~~:29:37 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 13 10:00:23 2009''

OCA at 08:29, 6 May 2009

2009-05-06T08:29:40Z

←Older revision		Revision as of 08:29, 6 May 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3fot.jpg\|left\|200px]]

-	The ~~entry 3fot~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3fot", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3fot\| PDB=3fot \| SCENE= }}

-	~~Authors: Garvey, G.S., Rayment, I., McCormick, S.P., Alexander, N.J.~~	+	===Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides===

-	Description: Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 14 12~~:55:24 2009''	+	==About this Structure==
		+	3FOT is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOT OCA].
		+	[[Category: Fusarium sporotrichioides]]
		+	[[Category: Alexander, N J.]]
		+	[[Category: Garvey, G S.]]
		+	[[Category: McCormick, S P.]]
		+	[[Category: Rayment, I.]]
		+	[[Category: Acetyltransferase]]
		+	[[Category: Bahd superfamily]]
		+	[[Category: Coenzyme some]]
		+	[[Category: Deoxynivalenol]]
		+	[[Category: Fusarium graminearum]]
		+	[[Category: Fusarium head blight]]
		+	[[Category: Fusarium sporotrichioide]]
		+	[[Category: T-2 toxin]]
		+	[[Category: Transferase]]
		+	[[Category: Trichothecene mycotoxin]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 11:29:37 2009''

OCA: New page: '''Unreleased structure''' The entry 3fot is ON HOLD Authors: Garvey, G.S., Rayment, I., McCormick, S.P., Alexander, N.J. Description: Structural and Functional Characterization of TRI...

2009-01-14T10:55:25Z

New page: '''Unreleased structure''' The entry 3fot is ON HOLD Authors: Garvey, G.S., Rayment, I., McCormick, S.P., Alexander, N.J. Description: Structural and Functional Characterization of TRI...

New page

'''Unreleased structure'''

The entry 3fot is ON HOLD

Authors: Garvey, G.S., Rayment, I., McCormick, S.P., Alexander, N.J.

Description: Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 14 12:55:24 2009''