3ft0 - Revision history

OCA at 15:32, 1 November 2023

2023-11-01T15:32:10Z

←Older revision		Revision as of 15:32, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3ft0' size='340' side='right'caption='[[3ft0]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3ft0' size='340' side='right'caption='[[3ft0]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ft0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ft0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FT0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[3fs9\|3fs9]]~~, [[~~3fsa~~\|~~3fsa]], [[3fsv\|3fsv]], [[3fsw\|3fsw]], [[3fsz\|3fsz~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>azu, PA4922 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~287~~ "~~Bacillus aeruginosus~~" (~~Schroeter 1872) Trevisan 1885]~~)</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ft0 OCA], [https://pdbe.org/3ft0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ft0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ft0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ft0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ft0 OCA], [https://pdbe.org/3ft0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ft0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ft0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ft0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.	+	[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Banfield, M J~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Cupredoxin-fold]]~~	+	[[Category: Banfield MJ]]
-	~~[[Category: Metal binding]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Protein:protein interaction~~]]	+

OCA at 08:10, 2 March 2022

2022-03-02T08:10:10Z

←Older revision		Revision as of 08:10, 2 March 2022
Line 1:		Line 1:

	==Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced==		==Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced==
-	<StructureSection load='3ft0' size='340' side='right' caption='[[3ft0]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3ft0' size='340' side='right'caption='[[3ft0]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ft0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ft0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FT0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fs9\|3fs9]], [[3fsa\|3fsa]], [[3fsv\|3fsv]], [[3fsw\|3fsw]], [[3fsz\|3fsz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fs9\|3fs9]], [[3fsa\|3fsa]], [[3fsv\|3fsv]], [[3fsw\|3fsw]], [[3fsz\|3fsz]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ft0 OCA], [~~http~~://pdbe.org/3ft0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ft0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ft0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ft0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ft0 OCA], [https://pdbe.org/3ft0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ft0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ft0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ft0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.	+	[[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[Azurin\|Azurin]]	+	*[[Azurin 3D structures\|Azurin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Banfield, M J]]		[[Category: Banfield, M J]]
	[[Category: Cupredoxin-fold]]		[[Category: Cupredoxin-fold]]

OCA at 07:55, 5 December 2018

2018-12-05T07:55:17Z

←Older revision		Revision as of 07:55, 5 December 2018
Line 1:		Line 1:
		+
	==Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced==		==Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced==
	<StructureSection load='3ft0' size='340' side='right' caption='[[3ft0]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3ft0' size='340' side='right' caption='[[3ft0]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fs9\|3fs9]], [[3fsa\|3fsa]], [[3fsv\|3fsv]], [[3fsw\|3fsw]], [[3fsz\|3fsz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fs9\|3fs9]], [[3fsa\|3fsa]], [[3fsv\|3fsv]], [[3fsw\|3fsw]], [[3fsz\|3fsz]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ft0 OCA], [http://pdbe.org/3ft0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ft0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ft0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ft0 OCA], [http://pdbe.org/3ft0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ft0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ft0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ft0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/3ft0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/3ft0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:19, 9 February 2016

2016-02-09T08:19:09Z

←Older revision		Revision as of 08:19, 9 February 2016
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fs9\|3fs9]], [[3fsa\|3fsa]], [[3fsv\|3fsv]], [[3fsw\|3fsw]], [[3fsz\|3fsz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fs9\|3fs9]], [[3fsa\|3fsa]], [[3fsv\|3fsv]], [[3fsw\|3fsw]], [[3fsz\|3fsz]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ft0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ft0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ft0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ft0 OCA], [http://pdbe.org/3ft0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ft0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ft0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ft0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ft0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 05:39, 25 December 2014

2014-12-25T05:39:30Z

←Older revision		Revision as of 05:39, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3ft0\| PDB=3ft0 \| SCENE= }}~~	+	==Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced==
-	===Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced===	+	<StructureSection load='3ft0' size='340' side='right' caption='[[3ft0]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19299503}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3ft0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FT0 FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fs9\|3fs9]], [[3fsa\|3fsa]], [[3fsv\|3fsv]], [[3fsw\|3fsw]], [[3fsz\|3fsz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ft0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ft0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ft0 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.		[[http://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/3ft0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The C-terminal copper-binding loop in the beta-barrel fold of the cupredoxin azurin has been replaced with a range of sequences containing alanine, glycine, and valine residues to assess the importance of amino acid composition and the length of this region. The introduction of 2 and 4 alanines between the coordinating Cys, His, and Met results in loop structures matching those in naturally occurring proteins with the same loop lengths. A loop with 4 alanines between the Cys and His and 3 between the His and Met ligands has a structure identical to that of the WT protein, whose loop is the same length. Loop structure is dictated by length and not sequence allowing the properties of the main surface patch for interactions with partners, to which the loop is a major contributor, to be optimized. Loops with 2 amino acids between the ligands using glycine, alanine, and valine residues have been compared. An empirical relationship is found between copper site protection by the loop and reduction potential. A loop adorned with 4 methyl groups is sufficient to protect the copper ion, enabling most sequences to adequately perform this task. The mutant with 3 alanine residues between the ligands forms a strand-swapped dimer in the crystal structure, an arrangement that has not, to our knowledge, been seen previously for this family of proteins. Cupredoxins function as redox shuttles and are required to be monomeric; therefore, none have evolved with a metal-binding loop of this length.
		+
		+	Metal-binding loop length and not sequence dictates structure.,Sato K, Li C, Salard I, Thompson AJ, Banfield MJ, Dennison C Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5616-21. Epub 2009 Mar 19. PMID:19299503<ref>PMID:19299503</ref>

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3ft0]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:019299503</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Azurin\|Azurin]]
-	[[Category: Banfield, M J.]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Banfield, M J]]
	[[Category: Cupredoxin-fold]]		[[Category: Cupredoxin-fold]]
	[[Category: Metal binding]]		[[Category: Metal binding]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Protein:protein interaction]]		[[Category: Protein:protein interaction]]

OCA at 07:48, 2 April 2014

2014-04-02T07:48:06Z

←Older revision		Revision as of 07:48, 2 April 2014
Line 1:		Line 1:
-	[[Image:3ft0.png\|left\|200px]]
-
	{{STRUCTURE_3ft0\| PDB=3ft0 \| SCENE= }}		{{STRUCTURE_3ft0\| PDB=3ft0 \| SCENE= }}
-
	===Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced===		===Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced===
		+	{{ABSTRACT_PUBMED_19299503}}

-	~~{{ABSTRACT_PUBMED_019299503}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.

	==About this Structure==		==About this Structure==
-	[[3ft0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA].	+	[[3ft0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA].
-	~~[[Category~~: ~~Pseudomonas aeruginosa]]~~	+
		+	==Reference==
		+	<ref group="xtra">PMID:019299503</ref><references group="xtra"/><references/>
	[[Category: Banfield, M J.]]		[[Category: Banfield, M J.]]
	[[Category: Cupredoxin-fold]]		[[Category: Cupredoxin-fold]]

OCA: Protected "3ft0" [edit=sysop:move=sysop]

2013-02-20T14:31:07Z

Protected "3ft0" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:31, 20 February 2013

OCA at 14:30, 20 February 2013

2013-02-20T14:30:57Z

←Older revision		Revision as of 14:30, 20 February 2013
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-	~~{{Seed}}~~	+	[[Image:3ft0.png\|left\|200px]]
-	[[Image:3ft0.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ft0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ft0\| PDB=3ft0 \| SCENE= }}		{{STRUCTURE_3ft0\| PDB=3ft0 \| SCENE= }}

	===Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced===		===Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced===

		+	{{ABSTRACT_PUBMED_019299503}}

	==About this Structure==		==About this Structure==
-	~~3FT0~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA].	+	[[3ft0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA].
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Banfield, M J.]]		[[Category: Banfield, M J.]]
-	[[Category: Copper]]
	[[Category: Cupredoxin-fold]]		[[Category: Cupredoxin-fold]]
	[[Category: Metal binding]]		[[Category: Metal binding]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Protein:protein interaction]]		[[Category: Protein:protein interaction]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 11 11:27:38 2009''

OCA at 09:27, 11 March 2009

2009-03-11T09:27:38Z

←Older revision		Revision as of 09:27, 11 March 2009
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3ft0.jpg\|left\|200px]]

-	The ~~entry 3ft0~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ft0", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ft0\| PDB=3ft0 \| SCENE= }}

-	~~Authors: Sato~~, ~~K., Li, C., Salard, I., Thompson, A.J., Banfield, M.J., Dennison, C.~~	+	===Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced===

-	Description: Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb~~ 11 12:35:36 2009''	+	==About this Structure==
		+	3FT0 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FT0 OCA].
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Banfield, M J.]]
		+	[[Category: Copper]]
		+	[[Category: Cupredoxin-fold]]
		+	[[Category: Metal binding]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Protein:protein interaction]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 11 11:27:38 2009''

OCA at 10:35, 11 February 2009

2009-02-11T10:35:36Z

←Older revision		Revision as of 10:35, 11 February 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ft0 is ON HOLD	+	The entry 3ft0 is ON HOLD until Paper Publication

	Authors: Sato, K., Li, C., Salard, I., Thompson, A.J., Banfield, M.J., Dennison, C.		Authors: Sato, K., Li, C., Salard, I., Thompson, A.J., Banfield, M.J., Dennison, C.
Line 7:		Line 7:
	Description: Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced		Description: Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 4 11:24:23 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 11 12:35:36 2009''