3fuo - Revision history

OCA at 06:52, 6 September 2023

2023-09-06T06:52:15Z

←Older revision		Revision as of 06:52, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3fuo' size='340' side='right'caption='[[3fuo]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3fuo' size='340' side='right'caption='[[3fuo]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fuo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FUO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fuo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FUO FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>atx, bna, botA, Botulinum Neurotoxin A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1491~~ "~~Bacillus botulinus~~" ~~van Ermengem 1896])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'~~>[~~https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin~~]~~, with EC number [https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [https://pdbe.org/3fuo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [https://www.ebi.ac.uk/pdbsum/3fuo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fuo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [https://pdbe.org/3fuo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [https://www.ebi.ac.uk/pdbsum/3fuo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fuo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~BXA1_CLOBO BXA1_CLOBO]~~] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.	+	[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus botulinus van ermengem 1896]]~~	+	[[Category: Clostridium botulinum]]
-	~~[[Category: Bontoxilysin~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baldwin~~, M R~~]]	+	[[Category: Baldwin MR]]
-	[[Category: Barbieri~~, J T~~]]	+	[[Category: Barbieri JT]]
-	[[Category: Chen, C]]	+	[[Category: Chen C]]
-	[[Category: Fu, Z]]	+	[[Category: Fu Z]]
-	[[Category: Kim, J ~~J.P]]~~	+	[[Category: Kim J-JP]]
-	~~[[Category: Botulinum neurotoxin]]~~	+
-	~~[[Category: Ganglioside binding]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Metal~~-~~binding]]~~	+
-	~~[[Category: Metalloprotease]]~~	+
-	~~[[Category: Neurotoxin]]~~	+
-	~~[[Category: Pharmaceutical]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Transmembrane]]~~	+
-	~~[[Category: Zinc~~]]	+

OCA at 08:11, 2 March 2022

2022-03-02T08:11:50Z

←Older revision		Revision as of 08:11, 2 March 2022
Line 1:		Line 1:

	==The Crystal structure of receptor binding domain of botulinum neurotoxin serotype A==		==The Crystal structure of receptor binding domain of botulinum neurotoxin serotype A==
-	<StructureSection load='3fuo' size='340' side='right' caption='[[3fuo]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3fuo' size='340' side='right'caption='[[3fuo]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fuo]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FUO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fuo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FUO FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atx, bna, botA, Botulinum Neurotoxin A ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atx, bna, botA, Botulinum Neurotoxin A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [~~http~~://pdbe.org/3fuo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fuo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fuo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [https://pdbe.org/3fuo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [https://www.ebi.ac.uk/pdbsum/3fuo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fuo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BXA1_CLOBO BXA1_CLOBO]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.	+	[[https://www.uniprot.org/uniprot/BXA1_CLOBO BXA1_CLOBO]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]	+	*[[Botulinum neurotoxin 3D structures\|Botulinum neurotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	[[Category: Bacillus botulinus van ermengem 1896]]		[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
		+	[[Category: Large Structures]]
	[[Category: Baldwin, M R]]		[[Category: Baldwin, M R]]
	[[Category: Barbieri, J T]]		[[Category: Barbieri, J T]]

OCA at 07:18, 5 December 2018

2018-12-05T07:18:00Z

←Older revision		Revision as of 07:18, 5 December 2018
Line 1:		Line 1:
		+
	==The Crystal structure of receptor binding domain of botulinum neurotoxin serotype A==		==The Crystal structure of receptor binding domain of botulinum neurotoxin serotype A==
	<StructureSection load='3fuo' size='340' side='right' caption='[[3fuo]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3fuo' size='340' side='right' caption='[[3fuo]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atx, bna, botA, Botulinum Neurotoxin A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atx, bna, botA, Botulinum Neurotoxin A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [http://pdbe.org/3fuo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [http://www.ebi.ac.uk/pdbsum/3fuo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [http://pdbe.org/3fuo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [http://www.ebi.ac.uk/pdbsum/3fuo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fuo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/3fuo_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/3fuo_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 49:		Line 50:
	[[Category: Metalloprotease]]		[[Category: Metalloprotease]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
		+	[[Category: Pharmaceutical]]
	[[Category: Protease]]		[[Category: Protease]]
	[[Category: Secreted]]		[[Category: Secreted]]
	[[Category: Toxin]]		[[Category: Toxin]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]
		+	[[Category: Zinc]]

OCA at 23:12, 9 February 2016

2016-02-09T23:12:48Z

←Older revision		Revision as of 23:12, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3fuo' size='340' side='right' caption='[[3fuo]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3fuo' size='340' side='right' caption='[[3fuo]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum Clostridium botulinum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FUO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FUO FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atx, bna, botA, Botulinum Neurotoxin A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 ~~Clostridium botulinum~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atx, bna, botA, Botulinum Neurotoxin A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [http://www.ebi.ac.uk/pdbsum/3fuo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [http://pdbe.org/3fuo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [http://www.ebi.ac.uk/pdbsum/3fuo PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fuo ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3fuo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
-	[[Category: Clostridium botulinum]]
	[[Category: Baldwin, M R]]		[[Category: Baldwin, M R]]
	[[Category: Barbieri, J T]]		[[Category: Barbieri, J T]]

OCA at 10:10, 25 December 2014

2014-12-25T10:10:05Z

←Older revision		Revision as of 10:10, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [http://www.ebi.ac.uk/pdbsum/3fuo PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [http://www.ebi.ac.uk/pdbsum/3fuo PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BXA1_CLOBO BXA1_CLOBO]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:06, 3 December 2014

2014-12-03T10:06:34Z

←Older revision		Revision as of 10:06, 3 December 2014
Line 1:		Line 1:
-	[[~~Image~~:3fuo.~~png\|left~~\|200px]]	+	==The Crystal structure of receptor binding domain of botulinum neurotoxin serotype A==
		+	<StructureSection load='3fuo' size='340' side='right' caption='[[3fuo]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3fuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FUO FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atx, bna, botA, Botulinum Neurotoxin A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fuo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fuo RCSB], [http://www.ebi.ac.uk/pdbsum/3fuo PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/3fuo_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Botulinum neurotoxin causes rapid flaccid paralysis through the inhibition of acetylcholine release at the neuromuscular junction. The seven BoNT serotypes (A-G) have been proposed to bind motor neurons via ganglioside-protein dual receptors. To date, the structure-function properties of BoNT/F host receptor interactions have not been resolved. Here, we report the crystal structures of the receptor binding domains (HCR) of BoNT/A and BoNT/F and the characterization of the dual receptors for BoNT/F. The overall polypeptide fold of HCR/A is essentially identical to the receptor binding domain of the BoNT/A holotoxin, and the structure of HCR/F is very similar to that of HCR/A, except for two regions implicated in neuronal binding. Solid phase array analysis identified two HCR/F binding glycans: ganglioside GD1a and oligosaccharides containing an N-acetyllactosamine core. Using affinity chromatography, HCR/F bound native synaptic vesicle glycoproteins as part of a protein complex. Deglycosylation of glycoproteins using alpha(1-3,4)-fucosidase, endo-beta-galactosidase, and PNGase F disrupted the interaction with HCR/F, while the binding of HCR/B to its cognate receptor, synaptotagmin I, was unaffected. These data indicate that the HCR/F binds synaptic vesicle glycoproteins through the keratan sulfate moiety of SV2. The interaction of HCR/F with gangliosides was also investigated. HCR/F bound specifically to gangliosides that contain alpha2,3-linked sialic acid on the terminal galactose of a neutral saccharide core (binding order GT1b = GD1a >> GM3; no binding to GD1b and GM1a). Mutations within the putative ganglioside binding pocket of HCR/F decreased binding to gangliosides, synaptic vesicle protein complexes, and primary rat hippocampal neurons. Thus, BoNT/F neuronal discrimination involves the recognition of ganglioside and protein (glycosylated SV2) carbohydrate moieties, providing a structural basis for the high affinity and specificity of BoNT/F for neurons.

-	~~{{STRUCTURE_3fuo\| PDB=3fuo \| SCENE= }}~~	+	Glycosylated SV2 and Gangliosides as Dual Receptors for Botulinum Neurotoxin Serotype F.,Fu Z, Chen C, Barbieri JT, Kim JJ, Baldwin MR Biochemistry. 2009 Jun 23;48(24):5631-41. PMID:19476346<ref>PMID:19476346</ref>

-	~~===The Crystal structure~~ of ~~receptor binding domain of botulinum neurotoxin serotype A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19476346}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3fuo]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_botulinum Clostridium botulinum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019476346<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
-	[[Category: Baldwin, M R.]]	+	[[Category: Baldwin, M R]]
-	[[Category: Barbieri, J T.]]	+	[[Category: Barbieri, J T]]
-	[[Category: Chen, C.]]	+	[[Category: Chen, C]]
-	[[Category: Fu, Z.]]	+	[[Category: Fu, Z]]
-	[[Category: Kim, J J.P.]]	+	[[Category: Kim, J J.P]]
	[[Category: Botulinum neurotoxin]]		[[Category: Botulinum neurotoxin]]
	[[Category: Ganglioside binding]]		[[Category: Ganglioside binding]]

OCA: Protected "3fuo" [edit=sysop:move=sysop]

2013-02-20T14:21:09Z

Protected "3fuo" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:21, 20 February 2013

OCA at 14:21, 20 February 2013

2013-02-20T14:21:07Z

←Older revision		Revision as of 14:21, 20 February 2013
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-	{{Seed}}
	[[Image:3fuo.png\|left\|200px]]		[[Image:3fuo.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3fuo", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3fuo\| PDB=3fuo \| SCENE= }}		{{STRUCTURE_3fuo\| PDB=3fuo \| SCENE= }}

	===The Crystal structure of receptor binding domain of botulinum neurotoxin serotype A===		===The Crystal structure of receptor binding domain of botulinum neurotoxin serotype A===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19476346}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19476346 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19476346}}		{{ABSTRACT_PUBMED_19476346}}

	==About this Structure==		==About this Structure==
-	~~3FUO~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA].	+	[[3fuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19476346~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019476346</ref><references group="xtra"/>
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
Line 38:		Line 26:
	[[Category: Metalloprotease]]		[[Category: Metalloprotease]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
-	[[Category: Pharmaceutical]]
	[[Category: Protease]]		[[Category: Protease]]
	[[Category: Secreted]]		[[Category: Secreted]]
	[[Category: Toxin]]		[[Category: Toxin]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]
-	[[Category: Zinc]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:23:20 2009''

OCA at 05:23, 25 June 2009

2009-06-25T05:23:22Z

←Older revision		Revision as of 05:23, 25 June 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3fuo.~~jpg~~\|left\|200px]]	+	[[Image:3fuo.png\|left\|200px]]

	<!--		<!--
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	[[Category: Zinc]]		[[Category: Zinc]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jun ~~17 10~~:09:36 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:23:20 2009''

OCA at 07:09, 17 June 2009

2009-06-17T07:09:39Z

←Older revision		Revision as of 07:09, 17 June 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3fuo.jpg\|left\|200px]]

-	The ~~entry 3fuo~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3fuo", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3fuo\| PDB=3fuo \| SCENE= }}

-	~~Authors: Fu, Z., Chen, C., Barbieri, J.T., Kim, J.-J.P., Baldwin, M.R.~~	+	===The Crystal structure of receptor binding domain of botulinum neurotoxin serotype A===

-	Description: The Crystal structure of receptor binding domain of botulinum neurotoxin serotype A

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 11 11:~~10:32 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19476346}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19476346 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19476346}}
		+
		+	==About this Structure==
		+	3FUO is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FUO OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19476346</ref><references group="xtra"/>
		+	[[Category: Bontoxilysin]]
		+	[[Category: Clostridium botulinum]]
		+	[[Category: Baldwin, M R.]]
		+	[[Category: Barbieri, J T.]]
		+	[[Category: Chen, C.]]
		+	[[Category: Fu, Z.]]
		+	[[Category: Kim, J J.P.]]
		+	[[Category: Botulinum neurotoxin]]
		+	[[Category: Ganglioside binding]]
		+	[[Category: Hydrolase]]
		+	[[Category: Membrane]]
		+	[[Category: Metal-binding]]
		+	[[Category: Metalloprotease]]
		+	[[Category: Neurotoxin]]
		+	[[Category: Pharmaceutical]]
		+	[[Category: Protease]]
		+	[[Category: Secreted]]
		+	[[Category: Toxin]]
		+	[[Category: Transmembrane]]
		+	[[Category: Zinc]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 17 10:09:36 2009''