3gmw - Revision history

OCA at 07:49, 16 March 2022

2022-03-16T07:49:14Z

←Older revision		Revision as of 07:49, 16 March 2022
Line 1:		Line 1:

	==Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase==		==Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase==
-	<StructureSection load='3gmw' size='340' side='right' caption='[[3gmw]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3gmw' size='340' side='right'caption='[[3gmw]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gmw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"actinomyces_exfoliatus"_waksman_and_curtis_1916 "actinomyces exfoliatus" waksman and curtis 1916] and [~~http~~://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3GMW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gmw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_exfoliatus"_waksman_and_curtis_1916 "actinomyces exfoliatus" waksman and curtis 1916] and [https://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GMW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gmu\|3gmu]], [[3gmv\|3gmv]], [[3gmx\|3gmx]], [[3gmy\|3gmy]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3gmu\|3gmu]], [[3gmv\|3gmv]], [[3gmx\|3gmx]], [[3gmy\|3gmy]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amp, bla ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=299586 Escherichia sp. Sflu5]), bliA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 "Actinomyces exfoliatus" Waksman and Curtis 1916])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amp, bla ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=299586 Escherichia sp. Sflu5]), bliA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 "Actinomyces exfoliatus" Waksman and Curtis 1916])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3gmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gmw OCA], [~~http~~://pdbe.org/3gmw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3gmw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3gmw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3gmw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gmw OCA], [https://pdbe.org/3gmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gmw RCSB], [https://www.ebi.ac.uk/pdbsum/3gmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gmw ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/3gmw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/3gmw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3gmw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3gmw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
		+	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 38:
	[[Category: Actinomyces exfoliatus waksman and curtis 1916]]		[[Category: Actinomyces exfoliatus waksman and curtis 1916]]
	[[Category: Escherichia sp. sflu5]]		[[Category: Escherichia sp. sflu5]]
		+	[[Category: Large Structures]]
	[[Category: Gretes, M]]		[[Category: Gretes, M]]
	[[Category: Lim, D C]]		[[Category: Lim, D C]]

OCA at 07:08, 1 November 2017

2017-11-01T07:08:07Z

←Older revision		Revision as of 07:08, 1 November 2017
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3gmw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3gmw" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]
-	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
	== References ==		== References ==
	<references/>		<references/>
Line 43:		Line 39:
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
		+	[[Category: Plasmid]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Protein-protein complex]]		[[Category: Protein-protein complex]]

OCA at 19:03, 11 August 2016

2016-08-11T19:03:55Z

←Older revision		Revision as of 19:03, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase==		==Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase==
	<StructureSection load='3gmw' size='340' side='right' caption='[[3gmw]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3gmw' size='340' side='right' caption='[[3gmw]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gmw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_sp._sflu5 Escherichia sp. sflu5~~] and [http://en.wikipedia.org/wiki/~~Streptomyces_exfoliatus Streptomyces exfoliatus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GMW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gmw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_exfoliatus"_waksman_and_curtis_1916 "actinomyces exfoliatus" waksman and curtis 1916] and [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GMW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gmu\|3gmu]], [[3gmv\|3gmv]], [[3gmx\|3gmx]], [[3gmy\|3gmy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gmu\|3gmu]], [[3gmv\|3gmv]], [[3gmx\|3gmx]], [[3gmy\|3gmy]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amp, bla ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=299586 Escherichia sp. Sflu5]), bliA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 ~~Streptomyces~~ exfoliatus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amp, bla ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=299586 Escherichia sp. Sflu5]), bliA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 "Actinomyces exfoliatus" Waksman and Curtis 1916])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gmw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gmw RCSB], [http://www.ebi.ac.uk/pdbsum/3gmw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gmw OCA], [http://pdbe.org/3gmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gmw RCSB], [http://www.ebi.ac.uk/pdbsum/3gmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gmw ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gmw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3gmw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actinomyces exfoliatus waksman and curtis 1916]]
	[[Category: Escherichia sp. sflu5]]		[[Category: Escherichia sp. sflu5]]
-	[[Category: Streptomyces exfoliatus]]
	[[Category: Gretes, M]]		[[Category: Gretes, M]]
	[[Category: Lim, D C]]		[[Category: Lim, D C]]

OCA at 09:23, 20 January 2015

2015-01-20T09:23:22Z

←Older revision		Revision as of 09:23, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3gmw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GMW FirstGlance]. <br>		<table><tr><td colspan='2'>[[3gmw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GMW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gmu\|3gmu]], [[3gmv\|3gmv]], [[3gmx\|3gmx]], [[3gmy\|3gmy]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gmu\|3gmu]], [[3gmv\|3gmv]], [[3gmx\|3gmx]], [[3gmy\|3gmy]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amp, bla ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=299586 Escherichia sp. Sflu5]), bliA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 Streptomyces exfoliatus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amp, bla ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=299586 Escherichia sp. Sflu5]), bliA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 Streptomyces exfoliatus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gmw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gmw RCSB], [http://www.ebi.ac.uk/pdbsum/3gmw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gmw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gmw RCSB], [http://www.ebi.ac.uk/pdbsum/3gmw PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Escherichia sp. sflu5]]		[[Category: Escherichia sp. sflu5]]
	[[Category: Streptomyces exfoliatus]]		[[Category: Streptomyces exfoliatus]]
-	[[Category: Gretes, M.]]	+	[[Category: Gretes, M]]
-	[[Category: Lim, D C.]]	+	[[Category: Lim, D C]]
-	[[Category: Strynadka, N C.J.]]	+	[[Category: Strynadka, N C.J]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Protein-protein complex]]		[[Category: Protein-protein complex]]

OCA at 13:11, 29 September 2014

2014-09-29T13:11:08Z

←Older revision		Revision as of 13:11, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3gmw.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase==
		+	<StructureSection load='3gmw' size='340' side='right' caption='[[3gmw]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3gmw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GMW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gmu\|3gmu]], [[3gmv\|3gmv]], [[3gmx\|3gmx]], [[3gmy\|3gmy]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amp, bla ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=299586 Escherichia sp. Sflu5]), bliA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 Streptomyces exfoliatus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gmw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gmw RCSB], [http://www.ebi.ac.uk/pdbsum/3gmw PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/3gmw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Beta-lactamase inhibitory protein (BLIP) binds a variety of beta-lactamase enzymes with wide-ranging specificity. Its binding mechanism and interface interactions are a well-established model system for the characterization of protein-protein interactions. Published studies have examined the binding of BLIP to diverse target beta-lactamases (e.g., TEM-1, SME-1, and SHV-1). However, apart from point mutations of amino acid residues, variability on the inhibitor side of this enzyme-inhibitor interface has remained unexplored. Thus, we present crystal structures of two likely BLIP relatives: (1) BLIP-I (solved alone and in complex with TEM-1), which has beta-lactamase inhibitory activity very similar to that of BLIP; and (2) beta-lactamase-inhibitory-protein-like protein (BLP) (in two apo forms, including an ultra-high-resolution structure), which is unable to inhibit any tested beta-lactamase. Despite categorical differences in species of origin and function, BLIP-I and BLP share nearly identical backbone conformations, even at loop regions differing in BLIP. We describe interacting residues and provide a comparative structural analysis of the interactions formed at the interface of BLIP-I.TEM-1 versus those formed at the interface of BLIP.TEM-1. Along with initial attempts to functionally characterize BLP, we examine its amino acid residues that structurally correspond to BLIP/BLIP-I binding hotspots to explain its inability to bind and inhibit TEM-1. We conclude that the BLIP family fold is a robust and flexible scaffold that permits the formation of high-affinity protein-protein interactions while remaining highly selective. Comparison of the two naturally occurring, distinct binding interfaces built upon this scaffold (BLIP and BLIP-I) shows that there is substantial variation possible in the subnanomolar binding interaction with TEM-1. The corresponding (non-TEM-1-binding) BLP surface shows that numerous favorable backbone-backbone/backbone-side-chain interactions with a protein partner can be negated by the presence of a few, strongly unfavorable interactions, especially electrostatic repulsions.

-	~~{{STRUCTURE_3gmw\| PDB=3gmw \| SCENE= }}~~	+	Insights into positive and negative requirements for protein-protein interactions by crystallographic analysis of the beta-lactamase inhibitory proteins BLIP, BLIP-I, and BLP.,Gretes M, Lim DC, de Castro L, Jensen SE, Kang SG, Lee KJ, Strynadka NC J Mol Biol. 2009 Jun 5;389(2):289-305. Epub 2009 Mar 28. PMID:19332077<ref>PMID:19332077</ref>

-	~~===Crystal Structure~~ of ~~Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19332077}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3gmw]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_sp~~.~~_sflu5 Escherichia sp. sflu5] and [http:~~/~~/en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA].~~	+

	==See Also==		==See Also==
	*[[Beta-lactamase\|Beta-lactamase]]		*[[Beta-lactamase\|Beta-lactamase]]
	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]		*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019332077<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia sp. sflu5]]		[[Category: Escherichia sp. sflu5]]
	[[Category: Streptomyces exfoliatus]]		[[Category: Streptomyces exfoliatus]]

OCA at 17:30, 20 October 2012

2012-10-20T17:30:03Z

←Older revision		Revision as of 17:30, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3gmw]] is a 4 chain structure ~~of [[Beta-lactamase]] and [[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA].	+	[[3gmw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA].

	==See Also==		==See Also==

OCA: Protected "3gmw" [edit=sysop:move=sysop]

2012-07-27T01:05:20Z

Protected "3gmw" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:05, 27 July 2012

OCA at 01:05, 27 July 2012

2012-07-27T01:05:18Z

←Older revision		Revision as of 01:05, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3gmw.png\|left\|200px]]		[[Image:3gmw.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3gmw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3gmw\| PDB=3gmw \| SCENE= }}		{{STRUCTURE_3gmw\| PDB=3gmw \| SCENE= }}

	===Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase===		===Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19332077}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19332077 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19332077}}		{{ABSTRACT_PUBMED_19332077}}

	==About this Structure==		==About this Structure==
-	~~3GMW~~ is a 4 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA].	+	[[3gmw]] is a 4 chain structure of [[Beta-lactamase]] and [[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA].
		+
		+	==See Also==
		+	*[[Beta-lactamase\|Beta-lactamase]]
		+	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19332077~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019332077</ref><references group="xtra"/>
	[[Category: Escherichia sp. sflu5]]		[[Category: Escherichia sp. sflu5]]
	[[Category: Streptomyces exfoliatus]]		[[Category: Streptomyces exfoliatus]]
Line 31:		Line 23:
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-	[[Category: Plasmid]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Protein-protein complex]]		[[Category: Protein-protein complex]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 15 09:26:47 2009''

OCA at 06:26, 15 April 2009

2009-04-15T06:26:47Z

←Older revision		Revision as of 06:26, 15 April 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3gmw.png\|left\|200px]]

-	The ~~entry 3gmw~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3gmw", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3gmw\| PDB=3gmw \| SCENE= }}

-	~~Authors: Lim, Daniel C., Gretes, Michael, Strynadka, Natalie C.J.~~	+	===Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase===

-	Description: Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Apr ~~9 12~~:17:36 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19332077}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19332077 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19332077}}
		+
		+	==About this Structure==
		+	3GMW is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19332077</ref><references group="xtra"/>
		+	[[Category: Escherichia sp. sflu5]]
		+	[[Category: Streptomyces exfoliatus]]
		+	[[Category: Gretes, M.]]
		+	[[Category: Lim, D C.]]
		+	[[Category: Strynadka, N C.J.]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Hydrolase]]
		+	[[Category: Plasmid]]
		+	[[Category: Protein binding]]
		+	[[Category: Protein-protein complex]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 15 09:26:47 2009''

OCA at 09:17, 9 April 2009

2009-04-09T09:17:36Z

←Older revision		Revision as of 09:17, 9 April 2009
Line 1:		Line 1:
-	~~{{Seed}}~~	+	'''Unreleased structure'''
-	~~[[Image:3gmw.jpg\|left\|200px]]~~	+

-	~~<!--~~	+	The entry 3gmw is ON HOLD
-	The ~~line below this paragraph, containing "STRUCTURE_3gmw", creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	~~-->~~	+
-	~~{{STRUCTURE_3gmw\| PDB=~~3gmw ~~\| SCENE= }}~~	+

-	~~===Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase===~~	+	Authors: Lim, Daniel C., Gretes, Michael, Strynadka, Natalie C.J.

		+	Description: Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Complex with TEM-1 Beta-Lactamase

-	~~==About this Structure==~~	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 9 12:17:36 2009''
-	3GMW is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_sp._sflu5 Escherichia sp. sflu5] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMW OCA].	+
-	~~[[Category: Escherichia sp. sflu5]]~~	+
-	~~[[Category: Streptomyces exfoliatus]]~~	+
-	~~[[Category: Gretes, M.]]~~	+
-	~~[[Category: Lim, D C.]]~~	+
-	~~[[Category: Strynadka, N C.J.]]~~	+
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Plasmid]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-		+
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr ~~2 16~~:15:03 2009''	+