3gni - Revision history

OCA at 07:08, 6 September 2023

2023-09-06T07:08:40Z

←Older revision		Revision as of 07:08, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3gni' size='340' side='right'caption='[[3gni]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='3gni' size='340' side='right'caption='[[3gni]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gni]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GNI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gni]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GNI FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ATP:ADENOSINE~~-~~5-TRIPHOSPHATE'>ATP</scene>~~, ~~<scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1upk\|1upk]], [[1upl\|1upl]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><td class="sblockDat">CAB39, MO25, CGI-66 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), STRADA, LYK5, STRAD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gni OCA], [https://pdbe.org/3gni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gni RCSB], [https://www.ebi.ac.uk/pdbsum/3gni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gni ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gni OCA], [https://pdbe.org/3gni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gni RCSB], [https://www.ebi.ac.uk/pdbsum/3gni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gni ProSAT]</span></td></tr>
	</table>		</table>
-	== Disease ==
-	[[https://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Note=A homozygous 7-kb deletion involving STRADA is a cause of a syndrome characterized by polyhydramnios, megalencephaly and symptomatic epilepsy.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CAB39_HUMAN CAB39_HUMAN]] Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1. [[https://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.<ref>PMID:12805220</ref> <ref>PMID:14517248</ref> <ref>PMID:19892943</ref>	+	[https://www.uniprot.org/uniprot/CAB39_HUMAN CAB39_HUMAN] Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Aalten, D M.F van]]~~	+	[[Category: Alessi DR]]
-	[[Category: Alessi~~, D R~~]]	+	[[Category: Goldie S]]
-	[[Category: Goldie, S]]	+	[[Category: Zeqiraj E]]
-	[[Category: Zeqiraj, E]]	+	[[Category: Van Aalten DMF]]
-	[[Category: ~~Adaptor protein]]~~	+
-	~~[[Category: Alpha helical repeat protein]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Kinase fold]]~~	+
-	~~[[Category: Metal binding protein-transferase complex]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Pseudokinase]]~~	+
-	~~[[Category: Signaling protein-signaling protein complex]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:49, 16 March 2022

2022-03-16T07:49:48Z

←Older revision		Revision as of 07:49, 16 March 2022
Line 1:		Line 1:

	==Structure of STRAD and MO25==		==Structure of STRAD and MO25==
-	<StructureSection load='3gni' size='340' side='right' caption='[[3gni]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='3gni' size='340' side='right'caption='[[3gni]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gni]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3GNI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gni]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GNI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1upk\|1upk]], [[1upl\|1upl]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1upk\|1upk]], [[1upl\|1upl]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAB39, MO25, CGI-66 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), STRADA, LYK5, STRAD ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAB39, MO25, CGI-66 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), STRADA, LYK5, STRAD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3gni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gni OCA], [~~http~~://pdbe.org/3gni PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3gni RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3gni PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3gni ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gni OCA], [https://pdbe.org/3gni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gni RCSB], [https://www.ebi.ac.uk/pdbsum/3gni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gni ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Note=A homozygous 7-kb deletion involving STRADA is a cause of a syndrome characterized by polyhydramnios, megalencephaly and symptomatic epilepsy.	+	[[https://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Note=A homozygous 7-kb deletion involving STRADA is a cause of a syndrome characterized by polyhydramnios, megalencephaly and symptomatic epilepsy.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CAB39_HUMAN CAB39_HUMAN]] Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1. [[~~http~~://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.<ref>PMID:12805220</ref> <ref>PMID:14517248</ref> <ref>PMID:19892943</ref>	+	[[https://www.uniprot.org/uniprot/CAB39_HUMAN CAB39_HUMAN]] Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1. [[https://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.<ref>PMID:12805220</ref> <ref>PMID:14517248</ref> <ref>PMID:19892943</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Aalten, D M.F van]]		[[Category: Aalten, D M.F van]]
	[[Category: Alessi, D R]]		[[Category: Alessi, D R]]

OCA at 05:37, 7 December 2018

2018-12-07T05:37:48Z

←Older revision		Revision as of 05:37, 7 December 2018
Line 17:		Line 17:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/3gni_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/3gni_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:34, 5 August 2016

2016-08-05T13:34:36Z

←Older revision		Revision as of 13:34, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of STRAD and MO25==		==Structure of STRAD and MO25==
	<StructureSection load='3gni' size='340' side='right' caption='[[3gni]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='3gni' size='340' side='right' caption='[[3gni]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gni]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GNI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gni]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GNI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1upk\|1upk]], [[1upl\|1upl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1upk\|1upk]], [[1upl\|1upl]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAB39, MO25, CGI-66 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), STRADA, LYK5, STRAD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAB39, MO25, CGI-66 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), STRADA, LYK5, STRAD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gni OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gni RCSB], [http://www.ebi.ac.uk/pdbsum/3gni PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gni OCA], [http://pdbe.org/3gni PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gni RCSB], [http://www.ebi.ac.uk/pdbsum/3gni PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gni ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 20:		Line 21:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gni ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 30:		Line 31:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3gni" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Aalten, D M.F van]]		[[Category: Aalten, D M.F van]]
	[[Category: Alessi, D R]]		[[Category: Alessi, D R]]

OCA at 15:11, 18 December 2014

2014-12-18T15:11:28Z

←Older revision		Revision as of 15:11, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3gni~~\| ~~PDB~~=3gni \| ~~SCENE~~= }}	+	==Structure of STRAD and MO25==
-	===~~Structure of STRAD and~~ MO25===	+	<StructureSection load='3gni' size='340' side='right' caption='[[3gni]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19513107}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3gni]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GNI FirstGlance]. <br>
-	==Disease==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1upk\|1upk]], [[1upl\|1upl]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAB39, MO25, CGI-66 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), STRADA, LYK5, STRAD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gni OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gni RCSB], [http://www.ebi.ac.uk/pdbsum/3gni PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
	[[http://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Note=A homozygous 7-kb deletion involving STRADA is a cause of a syndrome characterized by polyhydramnios, megalencephaly and symptomatic epilepsy.		[[http://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Note=A homozygous 7-kb deletion involving STRADA is a cause of a syndrome characterized by polyhydramnios, megalencephaly and symptomatic epilepsy.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAB39_HUMAN CAB39_HUMAN]] Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1. [[http://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.<ref>PMID:12805220</ref> <ref>PMID:14517248</ref> <ref>PMID:19892943</ref>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/3gni_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pseudokinases lack essential residues for kinase activity, yet are emerging as important regulators of signal transduction networks. The pseudokinase STRAD activates the LKB1 tumour suppressor by forming a heterotrimeric complex with LKB1 and the scaffolding protein MO25. Here, we describe the structure of STRADalpha in complex with MO25alpha. The structure reveals an intricate web of interactions between STRADalpha and MO25alpha involving the alphaC-helix of STRADalpha, reminiscent of the mechanism by which CDK2 interacts with cyclin A. Surprisingly, STRADalpha binds ATP and displays a closed conformation and an ordered activation loop, typical of active protein kinases. Inactivity is accounted for by nonconservative substitution of almost all essential catalytic residues. We demonstrate that binding of ATP enhances the affinity of STRADalpha for MO25alpha, and conversely, binding of MO25alpha promotes interaction of STRADalpha with ATP. Mutagenesis studies reveal that association of STRADalpha with either ATP or MO25alpha is essential for LKB1 activation. We conclude that ATP and MO25alpha cooperate to maintain STRADalpha in an "active" closed conformation required for LKB1 activation. It has recently been demonstrated that a mutation in human STRADalpha that truncates a C-terminal region of the pseudokinase domain leads to the polyhydramnios, megalencephaly, symptomatic epilepsy (PMSE) syndrome. We demonstrate this mutation destabilizes STRADalpha and prevents association with LKB1. In summary, our findings describe one of the first structures of a genuinely inactive pseudokinase. The ability of STRADalpha to activate LKB1 is dependent on a closed "active" conformation, aided by ATP and MO25alpha binding. Thus, the function of STRADalpha is mediated through an active kinase conformation rather than kinase activity. It is possible that other pseudokinases exert their function through nucleotide binding and active conformations.

-	~~==Function==~~	+	ATP and MO25alpha regulate the conformational state of the STRADalpha pseudokinase and activation of the LKB1 tumour suppressor.,Zeqiraj E, Filippi BM, Goldie S, Navratilova I, Boudeau J, Deak M, Alessi DR, van Aalten DM PLoS Biol. 2009 Jun 9;7(6):e1000126. Epub 2009 Jun 9. PMID:19513107<ref>PMID:19513107</ref>
-	~~[[http://www.uniprot.org/uniprot/CAB39_HUMAN CAB39_HUMAN]] Component of a complex that binds~~ and ~~activates STK11/LKB1. In~~ the ~~complex, required to stabilize~~ the ~~interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta)~~ and ~~STK11/~~LKB1. ~~[[http://www~~.~~uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Pseudokinase which, in complex with CAB39/MO25~~ (~~CAB39/MO25alpha or CAB39L/MO25beta~~)~~, binds to and activates STK11/LKB1~~. ~~Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation~~.~~<ref>~~PMID:~~12805220</ref>~~<ref>PMID:~~14517248~~</ref>~~<ref>PMID:19892943</ref>~~	+
-		+
-	~~==About this Structure==~~	+
-	[[3gni]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA].	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:019513107</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Aalten, D M.F van.]]	+	[[Category: Aalten, D M.F van]]
-	[[Category: Alessi, D R.]]	+	[[Category: Alessi, D R]]
-	[[Category: Goldie, S.]]	+	[[Category: Goldie, S]]
-	[[Category: Zeqiraj, E.]]	+	[[Category: Zeqiraj, E]]
	[[Category: Adaptor protein]]		[[Category: Adaptor protein]]
	[[Category: Alpha helical repeat protein]]		[[Category: Alpha helical repeat protein]]

OCA at 23:12, 24 March 2013

2013-03-24T23:12:48Z

←Older revision		Revision as of 23:12, 24 March 2013
Line 1:		Line 1:
-	[[Image:3gni.png\|left\|200px]]
-
	{{STRUCTURE_3gni\| PDB=3gni \| SCENE= }}		{{STRUCTURE_3gni\| PDB=3gni \| SCENE= }}
-
	===Structure of STRAD and MO25===		===Structure of STRAD and MO25===
		+	{{ABSTRACT_PUBMED_19513107}}

-	~~{{ABSTRACT_PUBMED_19513107}}~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Note=A homozygous 7-kb deletion involving STRADA is a cause of a syndrome characterized by polyhydramnios, megalencephaly and symptomatic epilepsy.
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/CAB39_HUMAN CAB39_HUMAN]] Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1. [[http://www.uniprot.org/uniprot/STRAA_HUMAN STRAA_HUMAN]] Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.<ref>PMID:12805220</ref><ref>PMID:14517248</ref><ref>PMID:19892943</ref>

	==About this Structure==		==About this Structure==
Line 11:		Line 13:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:019513107</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019513107</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Aalten, D M.F van.]]		[[Category: Aalten, D M.F van.]]

OCA: Protected "3gni" [edit=sysop:move=sysop]

2013-02-27T15:47:17Z

Protected "3gni" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:47, 27 February 2013

OCA at 15:47, 27 February 2013

2013-02-27T15:47:14Z

←Older revision		Revision as of 15:47, 27 February 2013
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-	{{Seed}}
	[[Image:3gni.png\|left\|200px]]		[[Image:3gni.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3gni", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_3gni\| PDB=3gni \| SCENE= }}		{{STRUCTURE_3gni\| PDB=3gni \| SCENE= }}

	===Structure of STRAD and MO25===		===Structure of STRAD and MO25===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19513107}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19513107 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_19513107}}		{{ABSTRACT_PUBMED_19513107}}

	==About this Structure==		==About this Structure==
-	~~3GNI~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA].	+	[[3gni]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19513107~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019513107</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Aalten, D M.F van.]]		[[Category: Aalten, D M.F van.]]
Line 31:		Line 19:
	[[Category: Adaptor protein]]		[[Category: Adaptor protein]]
	[[Category: Alpha helical repeat protein]]		[[Category: Alpha helical repeat protein]]
-	[[Category: Alternative splicing]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
-	[[Category: Chromosomal rearrangement]]
-	[[Category: Cytoplasm]]
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Kinase fold]]		[[Category: Kinase fold]]
-	[[Category: Metal binding protein/transferase complex]]	+	[[Category: Metal binding protein-transferase complex]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
	[[Category: Nucleus]]		[[Category: Nucleus]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
-	[[Category: Polymorphism]]
	[[Category: Pseudokinase]]		[[Category: Pseudokinase]]
-	[[Category: Signaling protein/signaling protein complex]]	+	[[Category: Signaling protein-signaling protein complex]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 09:20:41 2009''

OCA at 06:20, 25 June 2009

2009-06-25T06:20:43Z

←Older revision		Revision as of 06:20, 25 June 2009
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	===Structure of STRAD and MO25===		===Structure of STRAD and MO25===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19513107}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19513107 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19513107}}

	==About this Structure==		==About this Structure==
	3GNI is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA].		3GNI is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GNI OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19513107</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Aalten, D M.F van.]]		[[Category: Aalten, D M.F van.]]
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	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jun ~~24 20~~:34:31 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 09:20:41 2009''

OCA at 17:29, 24 June 2009

2009-06-24T17:29:54Z

←Older revision		Revision as of 17:29, 24 June 2009
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	{{Seed}}		{{Seed}}
-	[[Image:3gni.~~jpg~~\|left\|200px]]	+	[[Image:3gni.png\|left\|200px]]

	<!--		<!--
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	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun ~~17 11~~:30:05 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 24 20:34:31 2009''