3hi9 - Revision history

OCA at 07:22, 6 September 2023

2023-09-06T07:22:04Z

←Older revision		Revision as of 07:22, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3hi9' size='340' side='right'caption='[[3hi9]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3hi9' size='340' side='right'caption='[[3hi9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hi9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HI9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hi9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HI9 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1fxl\|1fxl]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">ELAVL1, HUR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi9 OCA], [https://pdbe.org/3hi9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hi9 RCSB], [https://www.ebi.ac.uk/pdbsum/3hi9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hi9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi9 OCA], [https://pdbe.org/3hi9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hi9 RCSB], [https://www.ebi.ac.uk/pdbsum/3hi9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hi9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ELAV1_HUMAN ELAV1_HUMAN]] Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro.<ref>PMID:19029303</ref>	+	[https://www.uniprot.org/uniprot/ELAV1_HUMAN ELAV1_HUMAN] Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro.<ref>PMID:19029303</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Benoit~~, R M~~]]	+	[[Category: Benoit RM]]
-	[[Category: Kallen, J]]	+	[[Category: Kallen J]]
-	~~[[Category: Beta1-alpha1-beta2-beta3-alpha2-beta4 topology]]~~	+
-	~~[[Category: Methylation]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Rna-binding]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 12:48, 23 March 2022

2022-03-23T12:48:35Z

←Older revision		Revision as of 12:48, 23 March 2022
Line 1:		Line 1:

	==The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution==		==The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution==
-	<StructureSection load='3hi9' size='340' side='right' caption='[[3hi9]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3hi9' size='340' side='right'caption='[[3hi9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hi9]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HI9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hi9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HI9 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fxl\|1fxl]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fxl\|1fxl]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ELAVL1, HUR ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ELAVL1, HUR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi9 OCA], [~~http~~://pdbe.org/3hi9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hi9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hi9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hi9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi9 OCA], [https://pdbe.org/3hi9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hi9 RCSB], [https://www.ebi.ac.uk/pdbsum/3hi9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hi9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ELAV1_HUMAN ELAV1_HUMAN]] Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro.<ref>PMID:19029303</ref>	+	[[https://www.uniprot.org/uniprot/ELAV1_HUMAN ELAV1_HUMAN]] Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro.<ref>PMID:19029303</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Benoit, R M]]		[[Category: Benoit, R M]]
	[[Category: Kallen, J]]		[[Category: Kallen, J]]

OCA at 08:08, 12 December 2018

2018-12-12T08:08:24Z

←Older revision		Revision as of 08:08, 12 December 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/3hi9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/3hi9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:01, 4 August 2016

2016-08-04T08:01:48Z

←Older revision		Revision as of 08:01, 4 August 2016
Line 1:		Line 1:
		+
	==The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution==		==The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution==
	<StructureSection load='3hi9' size='340' side='right' caption='[[3hi9]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3hi9' size='340' side='right' caption='[[3hi9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hi9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HI9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hi9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HI9 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fxl\|1fxl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fxl\|1fxl]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ELAVL1, HUR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ELAVL1, HUR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hi9 RCSB], [http://www.ebi.ac.uk/pdbsum/3hi9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi9 OCA], [http://pdbe.org/3hi9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hi9 RCSB], [http://www.ebi.ac.uk/pdbsum/3hi9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hi9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hi9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hi9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Benoit, R M]]		[[Category: Benoit, R M]]
	[[Category: Kallen, J]]		[[Category: Kallen, J]]

OCA at 07:21, 24 December 2014

2014-12-24T07:21:09Z

←Older revision		Revision as of 07:21, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hi9 RCSB], [http://www.ebi.ac.uk/pdbsum/3hi9 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hi9 RCSB], [http://www.ebi.ac.uk/pdbsum/3hi9 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ELAV1_HUMAN ELAV1_HUMAN]] Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro.<ref>PMID:19029303</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:15, 18 December 2014

2014-12-18T06:15:37Z

←Older revision		Revision as of 06:15, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3hi9\| PDB=3hi9 \| SCENE= }}~~	+	==The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution==
-	===The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution===	+	<StructureSection load='3hi9' size='340' side='right' caption='[[3hi9]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20219472}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hi9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HI9 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fxl\|1fxl]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ELAVL1, HUR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hi9 RCSB], [http://www.ebi.ac.uk/pdbsum/3hi9 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/3hi9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hu-antigen R (HuR) is a ubiquitous RNA-binding protein that comprises three RNA recognition motifs (RRMs). The first two tandem RRMs are known to bind to AU-rich elements (AREs) in the 3'-untranslated region of many mRNAs. The third RRM is connected to the second RRM through a basic hinge region that contains a localization signal termed HuR nucleocytoplasmic shuttling. Binding of HuR to the ARE in the 3'-untranslated region of mRNA leads to nuclear export, stabilization, and/or translational de-repression of the mRNA, resulting in upregulation of the encoded protein. Among the various ARE binding proteins known to date, HuR is still the only known ubiquitous antagonist of posttranscriptional gene silencing by AREs. Given the wide repertoire of known and suspected targets of HuR, it is considered to be a central node in the ARE pathway. Here, the x-ray crystal structure of the first RRM of HuR (amino acids 18-99) at 2.0 A resolution is presented. The overall fold consists of two alpha-helices and a four-stranded beta-sheet, with a beta1-alpha1-beta2-beta3-alpha2-beta4 topology and a beta-hairpin between alpha2 and beta4. The asymmetric unit consists of four chains. The large crystal contact interfaces observed between chains A/B and C/D contain hydrophobic residues located at the alpha-helix side of the fold, opposite to the RNA-binding interface. This hydrophobic region structurally resembles the protein-protein interaction site of RRM domains of other proteins. Because the nature of the assumed HuR homodimerization is mechanistically not well understood to date, we used site-directed mutagenesis, analytical size-exclusion chromatography and multiangle light scattering to investigate HuR interactions via the RRM hydrophobic region. Our data indicate that in vitro, HuR RRM1 and RRM1,2 homodimerization involves a disulfide bond at cysteine 13. This homodimerization mode may have a functional significance in redox modulation of HuR activity in response to oxidative stress. Because HuR is involved in many diseases (e.g., cancer, cachexia, and inflammatory bowel disease), the presented structure may provide a basis for rational drug design.

-	~~==About this Structure==~~	+	The x-ray crystal structure of the first RNA recognition motif and site-directed mutagenesis suggest a possible HuR redox sensing mechanism.,Benoit RM, Meisner NC, Kallen J, Graff P, Hemmig R, Cebe R, Ostermeier C, Widmer H, Auer M J Mol Biol. 2010 Apr 16;397(5):1231-44. Epub 2010 Feb 26. PMID:20219472<ref>PMID:20219472</ref>
-	~~[[3hi9]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3HI9 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	<~~ref group="xtra">PMID:020219472<~~/~~ref~~><~~references group="xtra"~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Benoit, R M.]]	+	[[Category: Benoit, R M]]
-	[[Category: Kallen, J.]]	+	[[Category: Kallen, J]]
	[[Category: Beta1-alpha1-beta2-beta3-alpha2-beta4 topology]]		[[Category: Beta1-alpha1-beta2-beta3-alpha2-beta4 topology]]
	[[Category: Methylation]]		[[Category: Methylation]]

OCA: Protected "3hi9" [edit=sysop:move=sysop]

2013-03-13T10:51:17Z

Protected "3hi9" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:51, 13 March 2013

OCA at 10:51, 13 March 2013

2013-03-13T10:51:15Z

←Older revision		Revision as of 10:51, 13 March 2013
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:3hi9.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3hi9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3hi9\| PDB=3hi9 \| SCENE= }}		{{STRUCTURE_3hi9\| PDB=3hi9 \| SCENE= }}
-
	===The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution===		===The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution===
-
-
-	<!--
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	{{ABSTRACT_PUBMED_20219472}}		{{ABSTRACT_PUBMED_20219472}}

	==About this Structure==		==About this Structure==
-	~~3HI9~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA].	+	[[3hi9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20219472~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020219472</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Benoit, R M.]]		[[Category: Benoit, R M.]]
Line 32:		Line 16:
	[[Category: Rna-binding]]		[[Category: Rna-binding]]
	[[Category: Transcription]]		[[Category: Transcription]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 7 10:08:30 2010''

OCA at 07:08, 7 April 2010

2010-04-07T07:08:30Z

←Older revision		Revision as of 07:08, 7 April 2010
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	{{Seed}}		{{Seed}}
-	[[Image:3hi9.~~jpg~~\|left\|200px]]	+	[[Image:3hi9.png\|left\|200px]]

	<!--		<!--
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	[[Category: Transcription]]		[[Category: Transcription]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 31 13~~:41:13 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 7 10:08:30 2010''

OCA at 10:41, 31 March 2010

2010-03-31T10:41:14Z

←Older revision		Revision as of 10:41, 31 March 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3hi9.jpg\|left\|200px]]

-	The ~~entry 3hi9~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3hi9", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3hi9\| PDB=3hi9 \| SCENE= }}

-	~~Authors: Benoit, R.M., Kallen, J~~.	+	===The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution===

-	Description: The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jun 10 17~~:59:~~12 2009~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20219472}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20219472 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20219472}}
		+
		+	==About this Structure==
		+	3HI9 is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI9 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20219472</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Benoit, R M.]]
		+	[[Category: Kallen, J.]]
		+	[[Category: Beta1-alpha1-beta2-beta3-alpha2-beta4 topology]]
		+	[[Category: Methylation]]
		+	[[Category: Phosphoprotein]]
		+	[[Category: Rna-binding]]
		+	[[Category: Transcription]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 31 13:41:13 2010''