3hpx - Revision history

OCA at 07:26, 6 September 2023

2023-09-06T07:26:22Z

←Older revision		Revision as of 07:26, 6 September 2023
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	<StructureSection load='3hpx' size='340' side='right'caption='[[3hpx]], [[Resolution\|resolution]] 2.03Å' scene=''>		<StructureSection load='3hpx' size='340' side='right'caption='[[3hpx]], [[Resolution\|resolution]] 2.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hpx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HPX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hpx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HPX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.03Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1sr9\|1sr9]], [[3fig\|3fig]], [[3hps\|3hps]], [[3hpz\|3hpz]], [[3hq1\|3hq1]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>leuA, MT3813, MTV025.058, Rv3710 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~83332 MYCTU])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/2-isopropylmalate_synthase 2-isopropylmalate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.13 2.3.3.13]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hpx OCA], [https://pdbe.org/3hpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hpx RCSB], [https://www.ebi.ac.uk/pdbsum/3hpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hpx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hpx OCA], [https://pdbe.org/3hpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hpx RCSB], [https://www.ebi.ac.uk/pdbsum/3hpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hpx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LEU1_MYCTU LEU1_MYCTU]] Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity).	+	[https://www.uniprot.org/uniprot/LEU1_MYCTU LEU1_MYCTU] Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 2-isopropylmalate synthase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Baker~~, E N~~]]	+	[[Category: Baker EN]]
-	[[Category: Koon, N]]	+	[[Category: Koon N]]
-	[[Category: Squire~~, C J]]~~	+	[[Category: Squire CJ]]
-	~~[[Category: Active site domain]]~~	+
-	~~[[Category: Amino-acid biosynthesis]]~~	+
-	~~[[Category: Branched-chain amino acid biosynthesis]]~~	+
-	~~[[Category: Leua]]~~	+
-	~~[[Category: Leucine biosynthesis]]~~	+
-	~~[[Category: Mycobacterium tuberculosis]]~~	+
-	~~[[Category: Tim barrel]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Truncation mutant~~]]	+

OCA at 11:54, 30 March 2022

2022-03-30T11:54:24Z

←Older revision		Revision as of 11:54, 30 March 2022
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	==Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)==		==Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)==
-	<StructureSection load='3hpx' size='340' side='right' caption='[[3hpx]], [[Resolution\|resolution]] 2.03Å' scene=''>	+	<StructureSection load='3hpx' size='340' side='right'caption='[[3hpx]], [[Resolution\|resolution]] 2.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hpx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HPX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hpx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HPX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sr9\|1sr9]], [[3fig\|3fig]], [[3hps\|3hps]], [[3hpz\|3hpz]], [[3hq1\|3hq1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1sr9\|1sr9]], [[3fig\|3fig]], [[3hps\|3hps]], [[3hpz\|3hpz]], [[3hq1\|3hq1]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">leuA, MT3813, MTV025.058, Rv3710 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">leuA, MT3813, MTV025.058, Rv3710 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/2-isopropylmalate_synthase 2-isopropylmalate synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.13 2.3.3.13] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/2-isopropylmalate_synthase 2-isopropylmalate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.13 2.3.3.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hpx OCA], [~~http~~://pdbe.org/3hpx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hpx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hpx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hpx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hpx OCA], [https://pdbe.org/3hpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hpx RCSB], [https://www.ebi.ac.uk/pdbsum/3hpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hpx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LEU1_MYCTU LEU1_MYCTU]] Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity).	+	[[https://www.uniprot.org/uniprot/LEU1_MYCTU LEU1_MYCTU]] Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[2-isopropylmalate synthase\|2-isopropylmalate synthase]]	+	*[[2-isopropylmalate synthase 3D structures\|2-isopropylmalate synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: 2-isopropylmalate synthase]]		[[Category: 2-isopropylmalate synthase]]
		+	[[Category: Large Structures]]
	[[Category: Myctu]]		[[Category: Myctu]]
	[[Category: Baker, E N]]		[[Category: Baker, E N]]

OCA at 07:36, 12 December 2018

2018-12-12T07:36:44Z

←Older revision		Revision as of 07:36, 12 December 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/3hpx_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/3hpx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	</div>		</div>
	<div class="pdbe-citations 3hpx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hpx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[2-isopropylmalate synthase\|2-isopropylmalate synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:16, 5 August 2016

2016-08-05T09:16:51Z

←Older revision		Revision as of 09:16, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)==		==Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)==
	<StructureSection load='3hpx' size='340' side='right' caption='[[3hpx]], [[Resolution\|resolution]] 2.03Å' scene=''>		<StructureSection load='3hpx' size='340' side='right' caption='[[3hpx]], [[Resolution\|resolution]] 2.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hpx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HPX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hpx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HPX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sr9\|1sr9]], [[3fig\|3fig]], [[3hps\|3hps]], [[3hpz\|3hpz]], [[3hq1\|3hq1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sr9\|1sr9]], [[3fig\|3fig]], [[3hps\|3hps]], [[3hpz\|3hpz]], [[3hq1\|3hq1]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">leuA, MT3813, MTV025.058, Rv3710 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 ~~Mycobacterium tuberculosis H37Rv~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">leuA, MT3813, MTV025.058, Rv3710 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-isopropylmalate_synthase 2-isopropylmalate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.13 2.3.3.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-isopropylmalate_synthase 2-isopropylmalate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.13 2.3.3.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hpx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hpx RCSB], [http://www.ebi.ac.uk/pdbsum/3hpx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hpx OCA], [http://pdbe.org/3hpx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hpx RCSB], [http://www.ebi.ac.uk/pdbsum/3hpx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hpx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hpx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hpx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: 2-isopropylmalate synthase]]		[[Category: 2-isopropylmalate synthase]]
-	[[Category: ~~Mycobacterium tuberculosis h37rv~~]]	+	[[Category: Myctu]]
	[[Category: Baker, E N]]		[[Category: Baker, E N]]
	[[Category: Koon, N]]		[[Category: Koon, N]]

OCA at 01:06, 25 December 2014

2014-12-25T01:06:09Z

←Older revision		Revision as of 01:06, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hpx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hpx RCSB], [http://www.ebi.ac.uk/pdbsum/3hpx PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hpx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hpx RCSB], [http://www.ebi.ac.uk/pdbsum/3hpx PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LEU1_MYCTU LEU1_MYCTU]] Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 15:48, 18 December 2014

2014-12-18T15:48:18Z

←Older revision		Revision as of 15:48, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3hpx\| PDB=3hpx \| SCENE= }}~~	+	==Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)==
-	===Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)===	+	<StructureSection load='3hpx' size='340' side='right' caption='[[3hpx]], [[Resolution\|resolution]] 2.03Å' scene=''>
-	~~{{ABSTRACT_PUBMED_22352945}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hpx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HPX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sr9\|1sr9]], [[3fig\|3fig]], [[3hps\|3hps]], [[3hpz\|3hpz]], [[3hq1\|3hq1]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">leuA, MT3813, MTV025.058, Rv3710 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium tuberculosis H37Rv])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-isopropylmalate_synthase 2-isopropylmalate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.13 2.3.3.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hpx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hpx RCSB], [http://www.ebi.ac.uk/pdbsum/3hpx PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/3hpx_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	alpha-Isopropylmalate synthase (alpha-IPMS) catalyzes the metal-dependent aldol reaction between alpha-ketoisovalerate (alpha-KIV) and acetyl-coenzyme A (AcCoA) to give alpha-isopropylmalate (alpha-IPM). This reaction is the first committed step in the biosynthesis of leucine in bacteria. alpha-IPMS is homodimeric, with monomers consisting of (beta/alpha)(8) barrel catalytic domains fused to a C-terminal regulatory domain, responsible for binding leucine and providing feedback regulation for leucine biosynthesis. In these studies, we demonstrate that removal of the regulatory domain from the alpha-IPMS enzymes of both Neisseria meningitidis (NmeIPMS) and Mycobacterium tuberculosis (MtuIPMS) results in enzymes that are unable to catalyze the formation of alpha-IPM, although truncated NmeIPMS was still able to slowly hydrolyze AcCoA. The lack of catalytic activity of these truncation variants was confirmed by complementation studies with Escherichia coli cells lacking the alpha-IPMS gene, where transformation with the plasmids encoding the truncated alpha-IPMS enzymes was not able to rescue alpha-IPMS activity. X-ray crystal structures of both truncation variants reveal that both proteins are dimeric and that the catalytic sites of the proteins are intact, although the divalent metal ion that is thought to be responsible for activating substrate alpha-KIV is displaced slightly relative to its position in the substrate-bound, wild-type structure. Isothermal titration calorimetry and WaterLOGSY nuclear magnetic resonance experiments demonstrate that although these truncation variants are not able to catalyze the reaction between alpha-KIV and AcCoA, they are still able to bind the substrate alpha-KIV. It is proposed that the regulatory domain is crucial for ensuring protein dynamics necessary for competent catalysis.

-	~~==Function==~~	+	Removal of the C-Terminal Regulatory Domain of alpha-Isopropylmalate Synthase Disrupts Functional Substrate Binding.,Huisman FH, Koon N, Bulloch EM, Baker HM, Baker EN, Squire CJ, Parker EJ Biochemistry. 2012 Mar 6. PMID:22352945<ref>PMID:22352945</ref>
-	~~[[http://www.uniprot.org/uniprot/LEU1_MYCTU LEU1_MYCTU]] Catalyzes the condensation~~ of the ~~acetyl group~~ of ~~acetyl~~-~~CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity)~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3hpx]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:022352945</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: 2-isopropylmalate synthase]]		[[Category: 2-isopropylmalate synthase]]
	[[Category: Mycobacterium tuberculosis h37rv]]		[[Category: Mycobacterium tuberculosis h37rv]]
-	[[Category: Baker, E N.]]	+	[[Category: Baker, E N]]
-	[[Category: Koon, N.]]	+	[[Category: Koon, N]]
-	[[Category: Squire, C J.]]	+	[[Category: Squire, C J]]
	[[Category: Active site domain]]		[[Category: Active site domain]]
	[[Category: Amino-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]

OCA at 07:34, 20 June 2013

2013-06-20T07:34:07Z

←Older revision		Revision as of 07:34, 20 June 2013
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-	[[Image:3hpx.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3hpx", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3hpx\| PDB=3hpx \| SCENE= }}		{{STRUCTURE_3hpx\| PDB=3hpx \| SCENE= }}
-
	===Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)===		===Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)===
		+	{{ABSTRACT_PUBMED_22352945}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/LEU1_MYCTU LEU1_MYCTU]] Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity).
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22352945}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 22352945 is~~ the ~~PubMed ID number.~~	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_22352945}}~~	+

	==About this Structure==		==About this Structure==
-	[[3hpx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis~~ Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA].	+	[[3hpx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:022352945</ref><references group="xtra"/>	+	<ref group="xtra">PMID:022352945</ref><references group="xtra"/><references/>
	[[Category: 2-isopropylmalate synthase]]		[[Category: 2-isopropylmalate synthase]]
-	[[Category: Mycobacterium tuberculosis]]	+	[[Category: Mycobacterium tuberculosis h37rv]]
	[[Category: Baker, E N.]]		[[Category: Baker, E N.]]
	[[Category: Koon, N.]]		[[Category: Koon, N.]]

OCA: Protected "3hpx" [edit=sysop:move=sysop]

2012-03-14T05:49:59Z

Protected "3hpx" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:49, 14 March 2012

OCA at 05:49, 14 March 2012

2012-03-14T05:49:57Z

←Older revision		Revision as of 05:49, 14 March 2012
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3hpx.png\|left\|200px]]
-	[[Image:3hpx.~~jpg~~\|left\|200px]]	+

	<!--		<!--
Line 12:		Line 11:
	===Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)===		===Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22352945}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22352945 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22352945}}

	==About this Structure==		==About this Structure==
-	~~3HPX~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis_h37rv~~ Mycobacterium tuberculosis ~~h37rv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA].	+	[[3hpx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022352945</ref><references group="xtra"/>
	[[Category: 2-isopropylmalate synthase]]		[[Category: 2-isopropylmalate synthase]]
-	[[Category: Mycobacterium tuberculosis ~~h37rv~~]]	+	[[Category: Mycobacterium tuberculosis]]
	[[Category: Baker, E N.]]		[[Category: Baker, E N.]]
	[[Category: Koon, N.]]		[[Category: Koon, N.]]
Line 29:		Line 37:
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Truncation mutant]]		[[Category: Truncation mutant]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 20 09:46:57 2010''

OCA at 06:46, 20 May 2010

2010-05-20T06:46:59Z

←Older revision		Revision as of 06:46, 20 May 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3hpx.jpg\|left\|200px]]

-	The ~~entry 3hpx~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3hpx", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3hpx\| PDB=3hpx \| SCENE= }}

-	~~Authors~~: ~~Koon, N., Squire, C.J., Baker, E.N.~~	+	===Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)===

-	Description: Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jun 17~~ 09:47:~~45 2009~~''	+	==About this Structure==
		+	3HPX is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HPX OCA].
		+	[[Category: 2-isopropylmalate synthase]]
		+	[[Category: Mycobacterium tuberculosis h37rv]]
		+	[[Category: Baker, E N.]]
		+	[[Category: Koon, N.]]
		+	[[Category: Squire, C J.]]
		+	[[Category: Active site domain]]
		+	[[Category: Amino-acid biosynthesis]]
		+	[[Category: Branched-chain amino acid biosynthesis]]
		+	[[Category: Leua]]
		+	[[Category: Leucine biosynthesis]]
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Tim barrel]]
		+	[[Category: Transferase]]
		+	[[Category: Truncation mutant]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 20 09:46:57 2010''