3i0q - Revision history

OCA at 07:36, 6 September 2023

2023-09-06T07:36:05Z

←Older revision		Revision as of 07:36, 6 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_serogroup_1 Legionella pneumophila serogroup 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_serogroup_1 Legionella pneumophila serogroup 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>~~, ~~<scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3i0o\|3i0o]], [[3i1a\|3i1a]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">aph, aph9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66976 Legionella pneumophila serogroup 1])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [https://pdbe.org/3i0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [https://www.ebi.ac.uk/pdbsum/3i0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i0q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [https://pdbe.org/3i0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [https://www.ebi.ac.uk/pdbsum/3i0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i0q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O06916_LEGPN O06916_LEGPN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 38:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Legionella pneumophila serogroup 1]]		[[Category: Legionella pneumophila serogroup 1]]
-	[[Category: Berghuis~~, A M~~]]	+	[[Category: Berghuis AM]]
-	[[Category: Fong~~, D H~~]]	+	[[Category: Fong DH]]
-	[[Category: Hwang, J Y]]	+	[[Category: Hwang J-Y]]
-	[[Category: Lemke~~, C T~~]]	+	[[Category: Lemke CT]]
-	[[Category: Xiong, B]]	+	[[Category: Xiong B]]
-	~~[[Category: Aminoglycoside phosphotransferase]]~~	+
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Protein kinase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:19, 6 April 2022

2022-04-06T11:19:37Z

←Older revision		Revision as of 11:19, 6 April 2022
Line 1:		Line 1:

	==Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia==		==Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia==
-	<StructureSection load='3i0q' size='340' side='right' caption='[[3i0q]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='3i0q' size='340' side='right'caption='[[3i0q]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Legionella_pneumophila_serogroup_1 Legionella pneumophila serogroup 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_serogroup_1 Legionella pneumophila serogroup 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i0o\|3i0o]], [[3i1a\|3i1a]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i0o\|3i0o]], [[3i1a\|3i1a]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66976 Legionella pneumophila serogroup 1])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66976 Legionella pneumophila serogroup 1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [~~http~~://pdbe.org/3i0q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3i0q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3i0q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [https://pdbe.org/3i0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [https://www.ebi.ac.uk/pdbsum/3i0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i0q ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Phosphotransferase\|Phosphotransferase]]	+	*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Legionella pneumophila serogroup 1]]		[[Category: Legionella pneumophila serogroup 1]]
	[[Category: Berghuis, A M]]		[[Category: Berghuis, A M]]

OCA at 07:46, 12 December 2018

2018-12-12T07:46:18Z

←Older revision		Revision as of 07:46, 12 December 2018
Line 1:		Line 1:
		+
	==Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia==		==Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia==
	<StructureSection load='3i0q' size='340' side='right' caption='[[3i0q]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3i0q' size='340' side='right' caption='[[3i0q]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i0o\|3i0o]], [[3i1a\|3i1a]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i0o\|3i0o]], [[3i1a\|3i1a]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66976 Legionella pneumophila serogroup 1])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66976 Legionella pneumophila serogroup 1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [http://pdbe.org/3i0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [http://www.ebi.ac.uk/pdbsum/3i0q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [http://pdbe.org/3i0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [http://www.ebi.ac.uk/pdbsum/3i0q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3i0q ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/3i0q_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/3i0q_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 02:06, 7 February 2016

2016-02-07T02:06:55Z

←Older revision		Revision as of 02:06, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3i0q' size='340' side='right' caption='[[3i0q]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3i0q' size='340' side='right' caption='[[3i0q]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Legionella_pneumophila~~ Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_serogroup_1 Legionella pneumophila serogroup 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i0o\|3i0o]], [[3i1a\|3i1a]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i0o\|3i0o]], [[3i1a\|3i1a]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~446~~ Legionella pneumophila])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66976 Legionella pneumophila serogroup 1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [http://www.ebi.ac.uk/pdbsum/3i0q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [http://pdbe.org/3i0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [http://www.ebi.ac.uk/pdbsum/3i0q PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i0q ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3i0q" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Legionella pneumophila]]	+	[[Category: Legionella pneumophila serogroup 1]]
	[[Category: Berghuis, A M]]		[[Category: Berghuis, A M]]
	[[Category: Fong, D H]]		[[Category: Fong, D H]]

OCA at 07:52, 20 January 2015

2015-01-20T07:52:08Z

←Older revision		Revision as of 07:52, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i0o\|3i0o]], [[3i1a\|3i1a]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i0o\|3i0o]], [[3i1a\|3i1a]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 Legionella pneumophila])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 Legionella pneumophila])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [http://www.ebi.ac.uk/pdbsum/3i0q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [http://www.ebi.ac.uk/pdbsum/3i0q PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Legionella pneumophila]]		[[Category: Legionella pneumophila]]
-	[[Category: Berghuis, A M.]]	+	[[Category: Berghuis, A M]]
-	[[Category: Fong, D H.]]	+	[[Category: Fong, D H]]
-	[[Category: Hwang, J Y.]]	+	[[Category: Hwang, J Y]]
-	[[Category: Lemke, C T.]]	+	[[Category: Lemke, C T]]
-	[[Category: Xiong, B.]]	+	[[Category: Xiong, B]]
	[[Category: Aminoglycoside phosphotransferase]]		[[Category: Aminoglycoside phosphotransferase]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Protein kinase]]		[[Category: Protein kinase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 10:56, 29 September 2014

2014-09-29T10:56:28Z

←Older revision		Revision as of 10:56, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3i0q.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia==
		+	<StructureSection load='3i0q' size='340' side='right' caption='[[3i0q]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3i0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I0Q FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i0o\|3i0o]], [[3i1a\|3i1a]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 Legionella pneumophila])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i0q RCSB], [http://www.ebi.ac.uk/pdbsum/3i0q PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/3i0q_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aminoglycoside phosphotransferases (APHs) constitute a diverse group of enzymes that are often the underlying cause of aminoglycoside resistance in the clinical setting. Several APHs have been extensively characterized, including the elucidation of the three-dimensional structure of two APH(3') isozymes and an APH(2'') enzyme. Although many APHs are plasmid-encoded and are capable of inactivating numerous 2-deoxystreptmaine aminoglycosides with multiple regiospecificity, APH(9)-Ia, isolated from Legionella pneumophila, is an unusual enzyme among the APH family for its chromosomal origin and its specificity for a single non-2-deoxystreptamine aminoglycoside substrate, spectinomycin. We describe here the crystal structures of APH(9)-Ia in its apo form, its binary complex with the nucleotide, AMP, and its ternary complex bound with ADP and spectinomycin. The structures reveal that APH(9)-Ia adopts the bilobal protein kinase-fold, analogous to the APH(3') and APH(2'') enzymes. However, APH(9)-Ia differs significantly from the other two types of APH enzymes in its substrate binding area and that it undergoes a conformation change upon ligand binding. Moreover, kinetic assay experiments indicate that APH(9)-Ia has stringent substrate specificity as it is unable to phosphorylate substrates of choline kinase or methylthioribose kinase despite high structural resemblance. The crystal structures of APH(9)-Ia demonstrate and expand our understanding of the diversity of the APH family, which in turn will facilitate the development of new antibiotics and inhibitors.

-	~~{{STRUCTURE_3i0q\| PDB=3i0q \| SCENE= }}~~	+	Structure of the antibiotic resistance factor spectinomycin phosphotransferase from Legionella pneumophila.,Fong DH, Lemke CT, Hwang J, Xiong B, Berghuis AM J Biol Chem. 2010 Mar 26;285(13):9545-55. Epub 2010 Jan 19. PMID:20089863<ref>PMID:20089863</ref>

-	~~===Crystal Structure~~ of the ~~AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20089863}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3i0q]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Legionella_pneumophila Legionella pneumophila]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA].~~	+

	==See Also==		==See Also==
	*[[Phosphotransferase\|Phosphotransferase]]		*[[Phosphotransferase\|Phosphotransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020089863<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Legionella pneumophila]]		[[Category: Legionella pneumophila]]
	[[Category: Berghuis, A M.]]		[[Category: Berghuis, A M.]]

OCA at 05:18, 20 October 2012

2012-10-20T05:18:05Z

←Older revision		Revision as of 05:18, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3i0q]] is a 1 chain structure ~~of [[Phosphotransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA].	+	[[3i0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA].

	==See Also==		==See Also==

OCA: Protected "3i0q" [edit=sysop:move=sysop]

2012-07-25T09:36:25Z

Protected "3i0q" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:36, 25 July 2012

OCA at 09:36, 25 July 2012

2012-07-25T09:36:24Z

←Older revision		Revision as of 09:36, 25 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3i0q.png\|left\|200px]]		[[Image:3i0q.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3i0q", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3i0q\| PDB=3i0q \| SCENE= }}		{{STRUCTURE_3i0q\| PDB=3i0q \| SCENE= }}

	===Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia===		===Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20089863}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20089863 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20089863}}		{{ABSTRACT_PUBMED_20089863}}

	==About this Structure==		==About this Structure==
-	~~3I0Q~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA].	+	[[3i0q]] is a 1 chain structure of [[Phosphotransferase]] with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA].
		+
		+	==See Also==
		+	*[[Phosphotransferase\|Phosphotransferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20089863~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020089863</ref><references group="xtra"/>
	[[Category: Legionella pneumophila]]		[[Category: Legionella pneumophila]]
	[[Category: Berghuis, A M.]]		[[Category: Berghuis, A M.]]
Line 34:		Line 25:
	[[Category: Protein kinase]]		[[Category: Protein kinase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 7 10:12:56 2010''

OCA at 07:12, 7 April 2010

2010-04-07T07:12:57Z

←Older revision		Revision as of 07:12, 7 April 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3i0q.~~jpg~~\|left\|200px]]	+	[[Image:3i0q.png\|left\|200px]]

	<!--		<!--
Line 12:		Line 12:
	===Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia===		===Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20089863}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20089863 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20089863}}

	==About this Structure==		==About this Structure==
	3I0Q is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA].		3I0Q is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0Q OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20089863</ref><references group="xtra"/>
	[[Category: Legionella pneumophila]]		[[Category: Legionella pneumophila]]
	[[Category: Berghuis, A M.]]		[[Category: Berghuis, A M.]]
Line 24:		Line 33:
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Protein kinase]]		[[Category: Protein kinase]]
		+	[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 20 15~~:48:10 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 7 10:12:56 2010''