3i9z - Revision history

OCA at 10:04, 21 February 2024

2024-02-21T10:04:30Z

←Older revision		Revision as of 10:04, 21 February 2024
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	<StructureSection load='3i9z' size='340' side='right'caption='[[3i9z]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3i9z' size='340' side='right'caption='[[3i9z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i9z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I9Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i9z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I9Z FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2qif\|2qif]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">BSU33510, copZ, COPZ_BACSU, yvgY ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [https://pdbe.org/3i9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [https://www.ebi.ac.uk/pdbsum/3i9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i9z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [https://pdbe.org/3i9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [https://www.ebi.ac.uk/pdbsum/3i9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i9z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/COPZ_BACSU COPZ_BACSU]] Chaperone that serves for the intracellular sequestration and transport of Cu(+). Delivers Cu(+) to the copper-transporting ATPase CopA.	+	[https://www.uniprot.org/uniprot/COPZ_BACSU COPZ_BACSU] Chaperone that serves for the intracellular sequestration and transport of Cu(+). Delivers Cu(+) to the copper-transporting ATPase CopA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i9z ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i9z ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Multinuclear Cu(I) clusters are common in nature, but little is known about their formation or transfer between proteins. CopZ and CopA from Bacillus subtilis, which are involved in a copper-efflux pathway, both readily accommodate multinuclear Cu(I) clusters. Using the luminescence properties of a multinuclear Cu(I)-bound form of the two N-terminal soluble domains of CopA (CopAab) we have investigated the thermodynamic and kinetic properties of cluster formation and loss. We demonstrate that Cu(I)-bound forms of dimeric CopZ containing more than one Cu(I) per CopZ monomer can transfer Cu(I) to apo-CopAab, leading to the formation of luminescent dimeric CopAab. Kinetic studies demonstrated that transfer is a first-order process and that the rate-determining steps for transfer from CopZ to CopAab and vice versa are different processes. The rate of formation of luminescent CopAab via transfer of Cu(I) from CopZ was more rapid than that observed when Cu(I) was added 'directly' from solution or in complex with a cysteine variant of CopZ, indicating that transfer occurs via a transient protein-protein complex. Such a complex would probably require the interaction of at least one domain of CopAab with the CopZ dimer. Insight into how such a complex might form is provided by the high resolution crystal structure of Cu3(CopZ)3, a thus far unique trimeric form of CopZ containing a trinuclear Cu(I) cluster. Modelling studies showed that one of the CopZ monomers can be substituted for either domain of CopAab, resulting in a heterotrimer, thus providing a model for a 'trapped' copper exchange complex.
-
-	Mechanistic insights into Cu(I) cluster transfer between the chaperone CopZ and its cognate Cu(I)-transporting P-type ATPase, CopA.,Singleton C, Hearnshaw S, Zhou L, Le Brun NE, Hemmings AM Biochem J. 2009 Dec 10;424(3):347-56. PMID:19751213<ref>PMID:19751213</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3i9z" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Brun, N E.Le~~]]	+	[[Category: Hearnshaw SJ]]
-	[[Category: ~~Hearnshaw, S J~~]]	+	[[Category: Hemmings AM]]
-	[[Category: ~~Hemmings, A M~~]]	+	[[Category: Le Brun NE]]
-	[[Category: Zhou, L]]	+	[[Category: Zhou L]]
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Copper]]~~	+
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Metal-binding~~]]	+

OCA at 11:01, 13 April 2022

2022-04-13T11:01:51Z

←Older revision		Revision as of 11:01, 13 April 2022
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	==Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster==		==Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster==
-	<StructureSection load='3i9z' size='340' side='right' caption='[[3i9z]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3i9z' size='340' side='right'caption='[[3i9z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i9z]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3I9Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i9z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I9Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qif\|2qif]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2qif\|2qif]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU33510, copZ, COPZ_BACSU, yvgY ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU33510, copZ, COPZ_BACSU, yvgY ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [~~http~~://pdbe.org/3i9z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3i9z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3i9z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [https://pdbe.org/3i9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [https://www.ebi.ac.uk/pdbsum/3i9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i9z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/COPZ_BACSU COPZ_BACSU]] Chaperone that serves for the intracellular sequestration and transport of Cu(+). Delivers Cu(+) to the copper-transporting ATPase CopA.	+	[[https://www.uniprot.org/uniprot/COPZ_BACSU COPZ_BACSU]] Chaperone that serves for the intracellular sequestration and transport of Cu(+). Delivers Cu(+) to the copper-transporting ATPase CopA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/3i9z_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/3i9z_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Vibrio subtilis ehrenberg 1835]]		[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Brun, N E.Le]]		[[Category: Brun, N E.Le]]
	[[Category: Hearnshaw, S J]]		[[Category: Hearnshaw, S J]]

OCA at 08:01, 1 November 2017

2017-11-01T08:01:00Z

←Older revision		Revision as of 08:01, 1 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster==		==Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster==
	<StructureSection load='3i9z' size='340' side='right' caption='[[3i9z]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3i9z' size='340' side='right' caption='[[3i9z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i9z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I9Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i9z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I9Z FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qif\|2qif]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qif\|2qif]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU33510, copZ, COPZ_BACSU, yvgY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "~~Bacillus globigii~~" ~~Migula 1900~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU33510, copZ, COPZ_BACSU, yvgY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [http://pdbe.org/3i9z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [http://www.ebi.ac.uk/pdbsum/3i9z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [http://pdbe.org/3i9z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [http://www.ebi.ac.uk/pdbsum/3i9z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3i9z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Brun, N E.Le]]		[[Category: Brun, N E.Le]]
	[[Category: Hearnshaw, S J]]		[[Category: Hearnshaw, S J]]
Line 39:		Line 40:
	[[Category: Zhou, L]]		[[Category: Zhou, L]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
		+	[[Category: Copper]]
		+	[[Category: Cytoplasm]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]

OCA at 15:28, 9 February 2016

2016-02-09T15:28:46Z

←Older revision		Revision as of 15:28, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3i9z' size='340' side='right' caption='[[3i9z]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3i9z' size='340' side='right' caption='[[3i9z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i9z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I9Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i9z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I9Z FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qif\|2qif]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qif\|2qif]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU33510, copZ, COPZ_BACSU, yvgY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus ~~subtilis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU33510, copZ, COPZ_BACSU, yvgY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [http://www.ebi.ac.uk/pdbsum/3i9z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [http://pdbe.org/3i9z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [http://www.ebi.ac.uk/pdbsum/3i9z PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i9z ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3i9z" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Brun, N E.Le]]		[[Category: Brun, N E.Le]]
	[[Category: Hearnshaw, S J]]		[[Category: Hearnshaw, S J]]

OCA at 07:44, 25 December 2014

2014-12-25T07:44:06Z

←Older revision		Revision as of 07:44, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [http://www.ebi.ac.uk/pdbsum/3i9z PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [http://www.ebi.ac.uk/pdbsum/3i9z PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/COPZ_BACSU COPZ_BACSU]] Chaperone that serves for the intracellular sequestration and transport of Cu(+). Delivers Cu(+) to the copper-transporting ATPase CopA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:16, 18 December 2014

2014-12-18T06:16:10Z

←Older revision		Revision as of 06:16, 18 December 2014
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-	~~{{STRUCTURE_3i9z~~\| ~~PDB~~=3i9z \| ~~SCENE~~= }}	+	==Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster==
-	===~~Crystal~~ structure of a ~~metallochaperone~~ with a trinuclear Cu(I) cluster~~===~~	+	<StructureSection load='3i9z' size='340' side='right' caption='[[3i9z]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19751213}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3i9z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I9Z FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qif\|2qif]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU33510, copZ, COPZ_BACSU, yvgY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i9z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i9z RCSB], [http://www.ebi.ac.uk/pdbsum/3i9z PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/3i9z_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multinuclear Cu(I) clusters are common in nature, but little is known about their formation or transfer between proteins. CopZ and CopA from Bacillus subtilis, which are involved in a copper-efflux pathway, both readily accommodate multinuclear Cu(I) clusters. Using the luminescence properties of a multinuclear Cu(I)-bound form of the two N-terminal soluble domains of CopA (CopAab) we have investigated the thermodynamic and kinetic properties of cluster formation and loss. We demonstrate that Cu(I)-bound forms of dimeric CopZ containing more than one Cu(I) per CopZ monomer can transfer Cu(I) to apo-CopAab, leading to the formation of luminescent dimeric CopAab. Kinetic studies demonstrated that transfer is a first-order process and that the rate-determining steps for transfer from CopZ to CopAab and vice versa are different processes. The rate of formation of luminescent CopAab via transfer of Cu(I) from CopZ was more rapid than that observed when Cu(I) was added 'directly' from solution or in complex with a cysteine variant of CopZ, indicating that transfer occurs via a transient protein-protein complex. Such a complex would probably require the interaction of at least one domain of CopAab with the CopZ dimer. Insight into how such a complex might form is provided by the high resolution crystal structure of Cu3(CopZ)3, a thus far unique trimeric form of CopZ containing a trinuclear Cu(I) cluster. Modelling studies showed that one of the CopZ monomers can be substituted for either domain of CopAab, resulting in a heterotrimer, thus providing a model for a 'trapped' copper exchange complex.

-	~~==About this Structure==~~	+	Mechanistic insights into Cu(I) cluster transfer between the chaperone CopZ and its cognate Cu(I)-transporting P-type ATPase, CopA.,Singleton C, Hearnshaw S, Zhou L, Le Brun NE, Hemmings AM Biochem J. 2009 Dec 10;424(3):347-56. PMID:19751213<ref>PMID:19751213</ref>
-	~~[[3i9z]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis Bacillus subtilis]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	<~~ref group="xtra">PMID:019751213<~~/~~ref~~><~~references group="xtra"~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
-	[[Category: Brun, N E.Le.]]	+	[[Category: Brun, N E.Le]]
-	[[Category: Hearnshaw, S J.]]	+	[[Category: Hearnshaw, S J]]
-	[[Category: Hemmings, A M.]]	+	[[Category: Hemmings, A M]]
-	[[Category: Zhou, L.]]	+	[[Category: Zhou, L]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]

OCA: Protected "3i9z" [edit=sysop:move=sysop]

2013-03-13T10:51:56Z

Protected "3i9z" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:51, 13 March 2013

OCA at 10:51, 13 March 2013

2013-03-13T10:51:53Z

←Older revision		Revision as of 10:51, 13 March 2013
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-	{{Seed}}
-	[[Image:3i9z.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3i9z", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3i9z\| PDB=3i9z \| SCENE= }}		{{STRUCTURE_3i9z\| PDB=3i9z \| SCENE= }}
-
	===Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster===		===Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19751213}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19751213 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19751213}}		{{ABSTRACT_PUBMED_19751213}}

	==About this Structure==		==About this Structure==
-	~~3I9Z~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA].	+	[[3i9z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19751213~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019751213</ref><references group="xtra"/>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Brun, N E.Le.]]		[[Category: Brun, N E.Le.]]
Line 30:		Line 14:
	[[Category: Zhou, L.]]		[[Category: Zhou, L.]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
-	[[Category: Copper]]
-	[[Category: Cytoplasm]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 9 14:06:49 2009''

OCA at 12:06, 9 December 2009

2009-12-09T12:06:50Z

←Older revision		Revision as of 12:06, 9 December 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3i9z.~~jpg~~\|left\|200px]]	+	[[Image:3i9z.png\|left\|200px]]

	<!--		<!--
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	[[Category: Metal-binding]]		[[Category: Metal-binding]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov 18 19~~:35:35 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 9 14:06:49 2009''

OCA at 17:35, 18 November 2009

2009-11-18T17:35:36Z

←Older revision		Revision as of 17:35, 18 November 2009
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3i9z.jpg\|left\|200px]]

-	The ~~entry 3i9z~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3i9z", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3i9z\| PDB=3i9z \| SCENE= }}

-	~~Authors: Hearnshaw, S.J., Zhou, L., Le Brun, N.E., Hemmings, A.M.~~	+	===Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster===

-	Description: Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 5 09~~:49:08 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19751213}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19751213 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19751213}}
		+
		+	==About this Structure==
		+	3I9Z is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I9Z OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19751213</ref><references group="xtra"/>
		+	[[Category: Bacillus subtilis]]
		+	[[Category: Brun, N E.Le.]]
		+	[[Category: Hearnshaw, S J.]]
		+	[[Category: Hemmings, A M.]]
		+	[[Category: Zhou, L.]]
		+	[[Category: Chaperone]]
		+	[[Category: Copper]]
		+	[[Category: Cytoplasm]]
		+	[[Category: Metal-binding]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 18 19:35:35 2009''