3ja9 - Revision history

OCA at 08:41, 7 February 2024

2024-02-07T08:41:54Z

←Older revision		Revision as of 08:41, 7 February 2024
Line 3:		Line 3:
	<SX load='3ja9' size='340' side='right' viewer='molstar' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>		<SX load='3ja9' size='340' side='right' viewer='molstar' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~PCNA (~~[~~https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 22Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja9 OCA], [https://pdbe.org/3ja9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ja9 RCSB], [https://www.ebi.ac.uk/pdbsum/3ja9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja9 OCA], [https://pdbe.org/3ja9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ja9 RCSB], [https://www.ebi.ac.uk/pdbsum/3ja9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>	+	[https://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Translesion synthesis (TLS) is the mechanism by which DNA polymerases replicate through unrepaired DNA lesions. TLS is activated by monoubiquitination of the homotrimeric proliferating cell nuclear antigen (PCNA) at lysine-164, followed by the switch from replicative to specialized polymerases at DNA damage sites. Pol eta belongs to the Y-Family of specialized polymerases that can efficiently bypass UV-induced lesions. Like other members of the Y-Family polymerases, its recruitment to the damaged sites is mediated by the interaction with monoubiquitinated PCNA (Ub-PCNA) via its ubiquitin-binding domain and non-canonical PCNA-interacting motif in the C-terminal region. The structural determinants underlying the direct recognition of Ub-PCNA by Pol eta, or Y-Family polymerases in general, remain largely unknown. Here we report a structure of the Ub-PCNA/Pol eta complex bound to DNA determined by single-particle electron microscopy (EM). The overall obtained structure resembles that of the editing PCNA/PolB complex. Analysis of the map revealed the conformation of ubiquitin that binds the C-terminal domain of Pol eta. Our present study suggests that the Ub-PCNA/Pol eta interaction requires the formation of a structured binding interface, which is dictated by the inherent flexibility of Ub-PCNA.	+
-		+
-	~~Molecular architecture of the Ub-PCNA/Pol eta complex bound to DNA.,Lau WC, Li Y, Zhang Q, Huen MS Sci Rep. 2015 Oct 27;5:15759. doi: 10.1038/srep15759. PMID:26503230<ref>PMID:26503230</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3ja9" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Huen~~, M S.Y~~]]	+	[[Category: Huen MSY]]
-	[[Category: Lau~~, W C.Y~~]]	+	[[Category: Lau WCY]]
-	[[Category: Li, Y]]	+	[[Category: Li Y]]
-	[[Category: Zhang, Q]]	+	[[Category: Zhang Q]]
-	~~[[Category: Dna]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Dna-binding systemic lupus erythematosus]]~~	+
-	~~[[Category: Oncogene]]~~	+
-	~~[[Category: Processivity]]~~	+
-	~~[[Category: Replication~~]]	+

OCA at 13:29, 4 May 2022

2022-05-04T13:29:33Z

←Older revision		Revision as of 13:29, 4 May 2022
Line 3:		Line 3:
	<SX load='3ja9' size='340' side='right' viewer='molstar' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>		<SX load='3ja9' size='340' side='right' viewer='molstar' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja9 OCA], [~~http~~://pdbe.org/3ja9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ja9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ja9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja9 OCA], [https://pdbe.org/3ja9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ja9 RCSB], [https://www.ebi.ac.uk/pdbsum/3ja9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>	+	[[https://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:18, 10 April 2020

2020-04-10T18:18:43Z

←Older revision		Revision as of 18:18, 10 April 2020
Line 3:		Line 3:
	<SX load='3ja9' size='340' side='right' viewer='molstar' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>		<SX load='3ja9' size='340' side='right' viewer='molstar' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja9 OCA], [http://pdbe.org/3ja9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ja9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ja9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja9 OCA], [http://pdbe.org/3ja9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ja9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ja9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:34, 6 March 2020

2020-03-06T17:34:13Z

←Older revision		Revision as of 17:34, 6 March 2020
Line 1:		Line 1:

	==Structure of native human PCNA==		==Structure of native human PCNA==
-	<~~StructureSection~~ load='3ja9' size='340' side='right'caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>	+	<SX load='3ja9' size='340' side='right' viewer='molstar' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>
Line 21:		Line 21:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 07:18, 23 October 2019

2019-10-23T07:18:21Z

←Older revision		Revision as of 07:18, 23 October 2019
Line 1:		Line 1:

	==Structure of native human PCNA==		==Structure of native human PCNA==
-	<StructureSection load='3ja9' size='340' side='right' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>	+	<StructureSection load='3ja9' size='340' side='right'caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Huen, M S.Y]]		[[Category: Huen, M S.Y]]
	[[Category: Lau, W C.Y]]		[[Category: Lau, W C.Y]]

OCA at 09:25, 19 December 2018

2018-12-19T09:25:32Z

←Older revision		Revision as of 09:25, 19 December 2018
Line 1:		Line 1:
		+
	==Structure of native human PCNA==		==Structure of native human PCNA==
	<StructureSection load='3ja9' size='340' side='right' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>		<StructureSection load='3ja9' size='340' side='right' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja9 OCA], [http://pdbe.org/3ja9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ja9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ja9 PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja9 OCA], [http://pdbe.org/3ja9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ja9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ja9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Huen, M S.Y]]		[[Category: Huen, M S.Y]]
	[[Category: Lau, W C.Y]]		[[Category: Lau, W C.Y]]

OCA at 13:42, 9 December 2015

2015-12-09T13:42:07Z

←Older revision		Revision as of 13:42, 9 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of native human PCNA==
		+	<StructureSection load='3ja9' size='340' side='right' caption='[[3ja9]], [[Resolution\|resolution]] 22.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ja9]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JA9 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja9 OCA], [http://pdbe.org/3ja9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ja9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ja9 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Translesion synthesis (TLS) is the mechanism by which DNA polymerases replicate through unrepaired DNA lesions. TLS is activated by monoubiquitination of the homotrimeric proliferating cell nuclear antigen (PCNA) at lysine-164, followed by the switch from replicative to specialized polymerases at DNA damage sites. Pol eta belongs to the Y-Family of specialized polymerases that can efficiently bypass UV-induced lesions. Like other members of the Y-Family polymerases, its recruitment to the damaged sites is mediated by the interaction with monoubiquitinated PCNA (Ub-PCNA) via its ubiquitin-binding domain and non-canonical PCNA-interacting motif in the C-terminal region. The structural determinants underlying the direct recognition of Ub-PCNA by Pol eta, or Y-Family polymerases in general, remain largely unknown. Here we report a structure of the Ub-PCNA/Pol eta complex bound to DNA determined by single-particle electron microscopy (EM). The overall obtained structure resembles that of the editing PCNA/PolB complex. Analysis of the map revealed the conformation of ubiquitin that binds the C-terminal domain of Pol eta. Our present study suggests that the Ub-PCNA/Pol eta interaction requires the formation of a structured binding interface, which is dictated by the inherent flexibility of Ub-PCNA.

-	~~The entry 3ja9 is ON HOLD until Paper Publication~~	+	Molecular architecture of the Ub-PCNA/Pol eta complex bound to DNA.,Lau WC, Li Y, Zhang Q, Huen MS Sci Rep. 2015 Oct 27;5:15759. doi: 10.1038/srep15759. PMID:26503230<ref>PMID:26503230</ref>

-	~~Authors: Lau~~, W.C.Y.~~, Li, Y., Zhang, Q.,~~ Huen, M.S.Y.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Structure of native human PCNA~~	+	<div class="pdbe-citations 3ja9" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Huen, M S.Y]]
		+	[[Category: Lau, W C.Y]]
	[[Category: Li, Y]]		[[Category: Li, Y]]
-	[[Category: Huen, M.S.Y]]
-	[[Category: Lau, W.C.Y]]
	[[Category: Zhang, Q]]		[[Category: Zhang, Q]]
		+	[[Category: Dna]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Dna-binding systemic lupus erythematosus]]
		+	[[Category: Oncogene]]
		+	[[Category: Processivity]]
		+	[[Category: Replication]]

OCA at 07:24, 24 June 2015

2015-06-24T07:24:06Z

←Older revision		Revision as of 07:24, 24 June 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ja9 is ON HOLD	+	The entry 3ja9 is ON HOLD until Paper Publication

	Authors: Lau, W.C.Y., Li, Y., Zhang, Q., Huen, M.S.Y.		Authors: Lau, W.C.Y., Li, Y., Zhang, Q., Huen, M.S.Y.

OCA at 07:21, 3 June 2015

2015-06-03T07:21:15Z

←Older revision		Revision as of 07:21, 3 June 2015
Line 3:		Line 3:
	The entry 3ja9 is ON HOLD		The entry 3ja9 is ON HOLD

-	Authors: ,, ,, ,, ,	+	Authors: Lau, W.C.Y., Li, Y., Zhang, Q., Huen, M.S.Y.

-	Description: ~~NATIVE HUMAN~~ PCNA	+	Description: Structure of native human PCNA
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ,, ,, ~~,, ,~~]]	+	[[Category: Li, Y]]
		+	[[Category: Huen, M.S.Y]]
		+	[[Category: Lau, W.C.Y]]
		+	[[Category: Zhang, Q]]

OCA: Protected "3ja9" [edit=sysop:move=sysop]

2015-05-27T10:41:40Z

Protected "3ja9" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:41, 27 May 2015